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Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model

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Bio-Design and Manufacturing 2024年第3期7卷 307-319页

作者： Sen Wang Siqi Yao Na Pei Luge Bai Zhiyan Hao Dichen Li jiankang He j.miguel oliveira Xiaoyan Xue Ling Wang Xinggang Mao State Key Laboratory for Manufacturing System Engineering Xi’an Jiaotong UniversityXi’an 710054China School of Mechanical Engineering Xi’an Jiaotong UniversityXi’an 710054China NMPA Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices Xi’an Jiaotong UniversityXi’an 710054China 3B’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3B’s-PT Government Associate Laboratory 4806-909 Braga/Guimar˜aesPortugal Department of Pharmacology School of PharmacyFourth Military Medical UniversityXi’an 710032China Department of Neurosurgery Xijing HospitalFourth Military Medical UniversityXi’an 710032China

Hypoxia is a typical feature of the tumor microenvironment,one of the most critical factors affecting cell behavior and tumor ***,the lack of tumor models able to precisely emulate natural brain tumor tissue has impeded the study of the effects of hypoxia on the progression and growth of tumor *** study reports a three-dimensional(3D)brain tumor model obtained by encapsulating U87MG(U87)cells in a hydrogel containing type I *** also documents the effect of various oxygen concentrations(1%,7%,and 21%)in the culture environment on U87 cell morphology,proliferation,viability,cell cycle,apoptosis rate,and ***,it compares two-dimensional(2D)and 3D *** comparison purposes,cells cultured in flat culture dishes were used as the control(2D model).Cells cultured in the 3D model proliferated more slowly but had a higher apoptosis rate and proportion of cells in the resting phase(G0 phase)/gap I phase(G1 phase)than those cultured in the 2D ***,the two models yielded significantly different cell ***,hypoxia(e.g.,1%O2)affected cell morphology,slowed cell growth,reduced cell viability,and increased the apoptosis rate in the 3D *** results indicate that the constructed 3D model is effective for investigating the effects of biological and chemical factors on cell morphology and function,and can be more representative of the tumor microenvironment than 2D culture *** developed 3D glioblastoma tumor model is equally applicable to other studies in pharmacology and pathology.

关键词： Hypoxia Glioma Three-dimensional glioma model In vitro

Translation through collaboration:practice applied in BAMOS project in in vivo testing of innovative osteochondral scaffolds

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Biomaterials Translational 2022年第2期3卷 102-104页

作者： Ricardo Donate Maryam Tamaddon Viviana Ribeiro Mario Monzón j.miguel oliveira Chaozong Liu Departamento de Ingeniería Mecánica Grupo de Investigación en Fabricación Integrada y AvanzadaUniversidad de Las Palmas de Gran CanariaLas PalmasSpain Division of Surgery&Interventional Science University College LondonRoyal National Orthopaedic HospitalStanmoreUK B’s Research Group I3B’s-Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoBarcoGuimarãesPortugal ICVS/3B’s-PT Government Associated Laboratory BragaGuimarãesPortugal.

Osteoarthritis is the most common chronic degenerative joint disease,recognized by the World Health Organization as a public health problem that affects millions of people *** project Biomaterials and Additive Manufacturing:Osteochondral Scaffold(BAMOS)innovation applied to osteoarthritis,funded under the frame of the Horizon 2020 Research and Innovation Staff Exchanges(RISE)program,aims to delay or avoid the use of joint replacements by developing novel cost-effective osteochondral scaffold technology for early intervention of *** multidisciplinary consortium of BAMOS,formed by international leading research centres,collaborates through research and innovation staff *** project covers all the stages of the development before the clinical trials:design of scaffolds,biomaterials development,processability under additive manufacturing,in vitro test,and in vivo *** paper reports the translational practice adopted in the project in in vivo assessment of the osteochondral scaffolds developed.

关键词： bone cartilage in vivo evaluation regenerative medicine tissue engineering

Highly sensitive ratiometric fluorescent fiber matrices for oxygen sensing with micrometer spatial resolution

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Bio-Design and Manufacturing 2024年第3期7卷 292-306页

作者： Giuliana Grasso Valentina Onesto Stefania Forciniti Eliana D’Amone Francesco Colella Lara Pierantoni Valeria Famà Giuseppe Gigli Rui L.Reis j.miguel oliveira Loretta L.del Mercato nstitute of Nanotechnology National Research Council(CNR-NANOTEC)c/o Campus Ecoteknevia Monteroni73100 LecceItaly Department of Mathematics and Physics“Ennio De Giorgi” University of Salentoc/o Campus Ecoteknevia Monteroni73100 LecceItaly 3B’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3B’s-PT Government Associate Laboratory 4806-909 Braga/GuimarãesPortugal Department of Experimental Medicine University of Salentoc/o Campus Ecoteknevia Monteroni73100 LecceItaly

Oxygen(O_(2))-sensing matrices are promising tools for the live monitoring of extracellular O_(2) consumption levels in long-term cell *** this study,ratiometric O_(2)-sensing membranes were prepared by electrospinning,an easy,low-cost,scalable,and robust method for fabricating ***(ε-caprolactone)and poly(dimethyl)siloxane polymers were blended with tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II)dichloride,which was used as the O_(2)-sensing probe,and rhodamine B isothiocyanate,which was used as the reference *** functionalized scaffolds were morphologically characterized by scanning electron microscopy,and their physicochemical profiles were obtained by Fourier transform infrared spectroscopy,thermogravimetric analysis,and water contact angle *** sensing capabilities were investigated by confocal laser scanning microscopy,performing photobleaching,reversibility,and calibration curve studies toward different dissolved O_(2)(DO)*** sensing nanofibers showed a high response to changes in DO concentrations in the physiological-pathological range from 0.5%to 20%and good stability under ratiometric *** addition,the sensing systems were highly biocompatible for cell growth promoting adhesiveness and growth of three cancer cell lines,namely metastatic melanoma cell line SK-MEL2,breast cancer cell line MCF-7,and pancreatic ductal adenocarcinoma cell line Panc-1,thus recreating a suitable biological environment in *** O_(2)-sensing biomaterials can potentially measure alterations in cell metabolism caused by changes in ambient O_(2)content during drug testing/validation and tissue regeneration processes.

关键词： Electrospinning Ruthenium(II)dichloride Oxygen sensors Ratiometric imaging Fluorescence

Osteochondral scaffolds for early treatment of cartilage defects in osteoarthritic joints:from bench to clinic

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Biomaterials Translational 2020年第1期1卷 3-17页

作者： Maryam Tamaddon Helena Gilja Ling Wang jmiguel oliveira Xiaodan Sun Rongwei Tan Chaozong Liu Institute of Orthopaedic&Musculoskeletal Science University College LondonRoyal National Orthopaedic HospitalLondonUK State Key Laboratory for Manufacturing System Engineering School of Mechanical EngineeringXi’an Jiaotong UniversityXi’anShaanxi ProvinceChina B’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAveParkPortugal ICVS/3B’s-PT Government Associate Laboratory Braga/GuimarãesPortugal The Discoveries Centre for Regenerative and Precision Medicine Headquarters at University of MinhoAveparkPortugal School of Materials Science and Engineering Tsinghua UniversityBeijingChina Shenzhen Lando Biomaterials Co.Ltd.Merchants Guangming Science Park ShenzhenGuangdong ProvinceChina

Osteoarthritis is a degenerative joint disease,typified by the loss in the quality of cartilage and bone at the interface of a synovial joint,resulting in pain,stiffness and reduced *** current surgical treatment for advanced stages of the disease is joint replacement,where the non-surgical therapeutic options or less invasive surgical treatments are no longer *** are major surgical procedures which have a substantial impact on patients’quality of life and lifetime risk of requiring revision *** using regenerative methods such as tissue engineering methods have been established and are promising for the early treatment of cartilage degeneration in osteoarthritis *** this approach,3-dimensional scaffolds(with or without cells)are employed to provide support for tissue ***,none of the currently available tissue engineering and regenerative medicine products promotes satisfactory durable regeneration of large cartilage ***,we discuss the current regenerative treatment options for cartilage and osteochondral(cartilage and underlying subchondral bone)defects in the articulating *** further identify the main hurdles in osteochondral scaffold development for achieving satisfactory and durable regeneration of osteochondral *** evolution of the osteochondral scaffolds–from monophasic to multiphasic constructs–is overviewed and the osteochondral scaffolds that have progressed to clinical trials are examined with respect to their clinical performances and their potential impact on the clinical *** of an osteochondral scaffold which bridges the gap between small defect treatment and joint replacement is still a grand *** scaffold could be used for early treatment of cartilage and osteochondral defects at early stage of osteoarthritis and could either negate or delay the need for joint replacements.

关键词： cartilage injury osteoarthritis osteochondral scaffold regenerative medicine subchondral defect tissue engineering

Deficit Irrigation as a Strategy to Save Water:Physiology and Potential Application to Horticulture

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journal of Integrative Plant Biology 2007年第10期49卷 1421-1434页

作者： j.miguel Costa Maria F.Ortuo M.Manuela Chaves InstitutoSuperior de Agronomia Universidade Técnica de LisboaTapada da Ajuda1349-017 LisboaPortugal Laboratório de Ecofisiologia MolecularInstituto de Tecnologia Química e BiológicaApartado 1272780-901 OeirasPortugal Instituto

Water is an increasingly scarce resource worldwide and irrigated agriculture remains one of the largest and most inefficient users of this resource. Low water use efficiency （WUE） together with an increased competition for water resources with other sectors （e.g. tourism or industry） are forcing growers to adopt new irrigation and cultivation practices that use water more judiciously. In areas with dry and hot climates, drip irrigation and protected cultivation have improved WUE mainly by reducing runoff and evapotranspiration losses. However, complementary approaches are still needed to increase WUE in irrigated agriculture. Deficit irrigation strategies like regulated deficit irrigation or partial root drying have emerged as potential ways to increase water savings in agriculture by allowing crops to withstand mild water stress with no or only marginal decreases of yield and quality. Grapevine and several fruit tree crops seem to be well adapted to deficit irrigation, but other crops like vegetables tend not to cope so well due to losses in yield and quality. This paper aims at providing an overview of the physiological basis of deficit irrigation strategies and their potential for horticulture by describing the major consequences of their use to vegetative growth, yield and quality of different crops （fruits, vegetables and ornamentals）.

关键词： deficit irrigation horticulture partial rootzone drying regulated deficit irrigation water saving.

Enzymatic and chemo-enzymatic strategies to produce highly valuable chiral amines from biomass with ω-transaminases on 2D zeolites

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National Science Review 2022年第9期9卷 10-18页

作者： j.miguel Carceller Karen S.Arias Maria j.Climent Sara Iborra Avelino Corma Instituto de Tecnologia Química (UPV-CSIC) Universitat Politècnica de València

Amino transaminases(ATAs) have been supported on a 2D ITQ-2 zeolite through electrostatic interactions, resulting in a highly stable active biocatalyst to obtain a variety of valuable chiral amines starting from prochiral ketones derived from biomass. We have extended the biocatalyst applications by designing a chemo-enzymatic process that allows, as the first step, prochiral ketones to be obtained from biomass-derived compounds through an aldol condensation–reduction step using a bifunctional metal/base catalyst. The prochiral ketone is subsequently converted into the chiral amine using the immobilized ATA. We show that it is feasible to couple both steps in a semi-continuous process to produce industrially relevant chiral amines with yields of >95% and ～100% enantiomer excess.

关键词： amino transaminase ITQ-2 zeolite chemo-enzymatic process biomass semi-continuous process Pd/MgO

Rat cardiomyocyte H9c2(2-1)-based sulforhodamine B assay as a promising in vitro method to assess the biological component of effluent toxicity

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journal of Environmental Sciences 2020年第10期32卷 163-170页

作者： Elsa T Rodrigues Susana F Nascimento Maria joao Moreno Paulo j oliveira miguel A Pardal University of Coimbra Centre for Functional EcologyDepartment of Life SciencesCalcada Martim de FreitasCoimbra 3000-456Portugal University of Coimbra Coimbra Chemistry CenterDepartment of ChemistryCoimbra 3004-535Portugal Centre for Neuroscience and Cell Biology Uniuersity of CoimbraUC BiotechBiocant ParkCantanhede 3060-197Portugal

The treatment of wastewaters is crucial to maintain the ecological status of receiving waters,and thereby guarantee the protection of aquatic life and human *** quality evaluation is conventionally based on physicochemical parameters,but increasing attention has been paid to integrate physicochemical and biological ***,the regulatory use of fish in biological testing methods has been subject to various ethical and cost concerns,and in vitro cell-based assays have thus become an important topic of ***,the present study intends:(a) to evaluate the efficiency of two different sample pre-concentration techniques (lyophilisation and solid phase extraction) to assess the toxicity of municipal effluents on rat cardiomyoblast H9c2(2-1) cells,and (b) maximizing the use of the effluent sample collected,to estimate the environmental condition of the receiving *** gathered results demonstrate that the H9c2(2-1) sulforhodamine B-based assay is an appropriate in vitro method to assess biological effluent toxicity,and the best results were attained by lyophilising the sample as *** to its response,the H9c2(2-1) cell line might be a possible alternative in vitro model for fish lethal testing to assess the toxicity of municipal *** physicochemical status of the sample suggests a high potential for eutrophication,and iron exceeded the permissible level for wastewater discharge,possibly due to the addition of ferric chloride for wastewater *** general,the levels of carbamazepine and sulfamethoxazole are higher than those reported for other countries,and both surpassed the aquatic protective values for long-term exposure.

关键词： Municipal wastewater treatment plant effluent Lyophilisation Solid phase extraction Cell-based assays H9c2(2-1)cell line SRB assay

Apples to oranges:environmentally derived,dynamic regulation of serotonin neuron subpopulations in adulthood?

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Neural Regeneration Research 2025年第9期20卷 2596-2597页

作者： Christopher j.O’Connell Matthew j.Robson Department of Genetics College of MedicineHarvard UniversityBostonMAUSA james L.Winkle College of Pharmacy Division of Pharmaceutical SciencesUniversity of CincinnatiCincinnatiOHUSA Neuroscience Graduate Program College of MedicineUniversity of CincinnatiCincinnatiOHUSA

Traumatic brain injury(TBI)is a public health problem with an undue economic burden that impacts nearly every age,ethnic,and gender group across the globe(Capizzi et al.,2020).TBIs are often sustained during a dynamic range of exposures to energetic environmental forces and as such outcomes are typically heterogeneous regarding severity and pathology(Capizzi et al.,2020).

关键词： sustained orange dynamic

Properties of Collagen/Sodium Alginate Hydrogels for Bioprinting of Skin Models

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journal of Bionic Engineering 2023年第1期20卷 105-118页

作者： Tian jiao Qin Lian Weilong Lian Yonghui Wang Dichen Li Rui L.Reis joaquim miguel oliveira State Key Laboratory for Manufacturing System Engineering Xi’an Jiaotong UniversityXi’an710049China Shaanxi Ketao-AM Technology Co. Ltd.Xi’an710100China B’s Research Group I3Bs—Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoBarco4805-017GuimarãesPortugal ICVS/3B’s–PT Government Associate Laboratory Barco4805-017GuimarãesPortugal

3D printing technology has great potential for the reconstruction of human ***,the reconstructed skin has some differences from natural skin,largely because the hydrogel used does not have the appropriate biological and physical properties to allow healing and *** study examines the swelling,degradability,microstructure and biological properties of Collagen/Sodium Alginate(Col/SA)hydrogels of differing compositions for the purposes of skin *** the content of sodium alginate causes the hydrogel to exhibit stronger mechanical and swelling properties,a faster degradation ratio,smaller pore size,and less favorable biological *** optimal 1%collagen hydrogel was used to print bi-layer skin in which fibroblasts and keratinocytes showed improved spreading and proliferation as compared to other developed *** Col/SA hydrogels presented suitable tunability and properties to be used as a bioink for bioprinting of skin aiming at finding applications as 3D models for wound healing research.

关键词： Bioink Bioprinting-Collagen sodium alginate hydrogel-Skin Wound healing Bionic