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Particle elasticity influences polymeric artificial antigen presenting cell effectiveness in vivo via CD8+T cell activation,macrophage uptake,and the protein corona

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Nano Research 2024年第10期17卷 9052-9064页

作者： Savannah E.Est-Witte Sydney R.Shannon Dennis H.Gong Kaitlyn G.Calabresi jawaun j.Harris Kaitlyn Storm Edwin j.yoo Ariel Isser Vivek P.jani Natalie K.Livingston Mary O.Omotoso Kelly Rhodes Elana Ben-Akiva Randall A.Meyer Zoe T.Hsieh Simone Sidoli Stephany Y.Tzeng jonathan P.Schneck jordan j.Green Translational Tissue Engineering Center Institute for NanobiotechnologyDepartment of Biomedical EngineeringJohns Hopkins School of Medicine400 N BroadwayBaltimoreMD 21231USA Institute for Cell Engineering Department of PathologyJohns Hopkins School of Medicine733 N BroadwayBaltimoreMD 21205USA Department of Biochemistry Albert Einstein College of MedicineThe BronxNew York CityNY 10461USA

Adoptive cell therapy(ACT)is an immunotherapy strategy for cancer that has seen widespread clinical *** ACT,patient-derived lymphocytes are stimulated with the antigen of interest ex vivo,proliferated,then returned to the patient to initiate an antigen-specific antitumor *** effective,this process is resource-intensive and logistically impossible for many *** artificial antigen presenting cells(aAPCs)offer a potential“off-the-shelf”alternative to ex vivo *** particulate aAPCs perform well in vitro,they have had limited success in vivo due to poor bioavailability after *** to bioavailability include rapid clearance,unfavorable biodistribution,and inadequate interactions with CD8+T cells at sites of *** properties such as elasticity have been shown to vastly impact the bioavailability and particle-cell interactions,but this has yet to be investigated in the context of aAPCs for in vivo T-cell *** literature likewise indicates that biomaterial properties,especially elasticity,can modulate T-cell activation in *** the goal of creating a more biomimetic,next-generation particulate aAPC,we developed a poly(ethylene)glycol hydrogel particle platform with tunable elasticity to investigate the impact of elasticity on antigen-specific T cell activation for in vivo adoptive *** this knowledge,we were able to gain more precise control over in vivo T cell activation and investigate possible mechanisms including the effects of aAPC elasticity on T cell binding,macrophage uptake,and the protein corona.

关键词： adoptive cell therapy artificial antigen presenting cells particle drug delivery elasticity protein corona poly(ethylene glycol)

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Leading Approaches to Vascularize Kidney Constructs in Tissue Engineering

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Engineering 2022年第12期19卷 117-127页

作者： Diana S.Lim john D.jackson Anthony Atala james j.yoo Wake Forest Institute for Regenerative Medicine Wake Forest University School of MedicineMedical Center BoulevardNC 27157USA

There is an unprecedented need for new treatments for renal failure,as the incidence of this disease is increasing disproportionately to advancements in *** treatments are limited by the availability of viable organs,for which there is a worldwide *** treatment modalities also require a substantial amount of infrastructure,significantly limiting the access to care in most *** tissue engineering approaches promise to develop alternative solutions that address many of the inadequacies in current *** many advancements have been made—primarily in the past decade—in biofabrication and whole-organ tissue engineering,many challenges *** major hindrance to the progress of current tissue engineering approaches is establishing successful vascularization of developed engineered tissue *** review focuses on the recent advancements that address the vascular challenge,including the biofabrication of vasculature,whole-organ engineering through decellularization and recellularization approaches,microscale organogenesis,and vascularization using organoids in the context of kidney tissue *** also highlight the specific challenges that remain in developing successful strategies capable of clinical translation.

关键词： Kidney Vascularization Tissue engineering Biofabrication Organoids

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Adenosine-treated bioprinted muscle constructs prolong cell survival and improve tissue formation

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Bio-Design and Manufacturing 2021年第3期4卷 441-451页

作者： Dongxu Ke Adam M.jorgensen Sang j.Lee james j.yoo Sean V.Murphy Wake Forest Institute for Regenerative Medicine Wake Forest School of MedicineWinston-SalemNCUSA Novaprint Therapeutics Suzhou Co. LtdSuzhouJiangsuChina

It is crucial to maintaining the viability of biofabricated human-sized tissues to ensure their successful survival and function after *** is a purine nucleoside that has the function to suppress cellular metabolism and has been previously proposed as a method to prolong cell viability under *** this study,we optimized the dose concentration of adenosine for incorporation into bioprinted constructs to preserve long-term cell viability in *** results showed that muscle cells(C2C12)containing 6,7,or 8 mM adenosine maintained high cell viability for 20 days under hypoxic conditions(0.1%O2),whereas cells without adenosine treatment showed 100%cell death after 11 *** 20 days under hypoxic conditions,muscle cells treated with adenosine proliferated and differentiated when transferred to normoxic *** these adenosine concentrations,6 mM was picked as the optimized adenosine concentration for further investigations due to its most effective results on improving cell *** bioprinted muscle constructs containing adenosine(6 mM)maintained high cell viability for 11 days under hypoxic conditions,while the control constructs without adenosine had no live *** in vivo validation,the bioprinted constructs with adenosine implanted under the dorsal subcutaneous space in mice,showed the enhanced formation of muscle tissue with minimal central necrosis and apoptosis,when compared to the constructs without *** positive in vitro and in vivo results demonstrate that the use of adenosine is an effective approach to preventing the challenge of hypoxia-induced necrosis in bioprinted tissues for clinical translation.

关键词： Bioprinting Hypoxia Adenosine Cell viability In vivo

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Re nanoclusters anchored on nanosheet supports: Formation of Re-O-matrix bonding and evaluation as all-pH-range hydrogen evolution reaction (HER) electrocatalysts

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journal of Energy Chemistry 2022年第6期31卷 185-193,I0006页

作者： Shiyu Xu Hao Li jeongbok Lee N.Clament Sagaya Selvam Baotao Kang jin Yong Lee Pil j.yoo School of Chemical Engineering Sungkyunkwan UniversitySuwon 16419Republic of Korea Department of Chemistry Sungkyunkwan UniversitySuwon 16419Republic of Korea

Although the water splitting-based generation of hydrogen as an energy carrier can help to mitigate the global problems of energy shortage and climate change,the practical implementation of this strategy is hindered by the absence of inexpensive high-performance electrocatalysts for the hydrogen evolution reaction (HER).Re-based HER electrocatalysts exhibit predictable high performance within the entire pH range but suffer from arduous formation (i.e.,vulnerability to oxidation) and uncontrollable aggregation,which strongly discourages the maximisation of active site exposure required for activity *** overcome these limitations,we herein hydrothermally synthesise Re nanoclusters uniformly distributed on nanosheet supports,such as reduced graphene oxide nanosheets (Re NCs@rGO),revealing that this hybrid features abundant exposed active sites and high oxidation *** obtained electrocatalysts were elaborately characterized by microscopic and spectroscopic ***,density functional theory calculations confirm the optimised synthesis of Re NCs@rGO and indicate the crucial role of Re–O–C junction formation in securing *** effective suppression of Re nanocluster detachment/dissolution under HER conditions endows Re NCs@rGO with high electron conductivity and electrochemical stability,resulting in a durability superior to that of commercial Pt/C and an activity similar to that of this *** a result,Re NCs@rGO exhibited remarkably small HER overpotentials of 110,130,and 93 m V to deliver a current density of 10 mA cm^(-2) in 0.5 M H_(2)SO_(4),1 M PBS,and 1 M KOH,***,Re NCs@rGO is a promising alternative to conventional Pt-group-metal catalysts and should find applications in next-generation high-performance water splitting systems.

关键词： Hydrogen evolution reaction(HER) Hydrothermal synthesis Re nanoclusters Nanosheet supports Electrocatalysts

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Electrochemical determination of the degree of atomic surface roughness in Pt-Ni alloy nanocatalysts for oxygen reduction reaction

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Carbon Energy 2021年第2期3卷 375-383页

作者： Tae-Yeol jeon Seung-Ho Yu Sung j.yoo Hee-Young Park Sang-Kyung Kim Beamline Department Pohang Accelerator Laboratory(PAL)PohangRepublic of Korea Department of Chemical and Biological Engineering Korea UniversitySeoulRepublic of Korea Fuel Cell Center Korea Institute of Science and Technology(KIST)SeoulRepublic of Korea Fuel Cell Research Center Korea Institute of Energy Research(KIER)DaejeonRepublic of Korea

Pt-Ni alloy nanocrystals with Pt-enriched shells were prepared by selective etching of surface Ni using sulfuric acid and *** changes in the electronic and geometric structure of the alloy nanoparticles at the surface were elucidated from the electrochemical surface area,the potential of zero total charge(PZTC),and relative surface roughness,which were determined from CO-and CO_(2)-displacement experiments before and after 3000 potential cycles under oxygen reduction reaction *** the highest activity and durability were achieved in hydroquinone-treated Pt–Ni,sulfuric acidtreated one showed the lower activity and durability despite its higher surface Pt concentration and alloying *** PZTC and QCO_(2)/QCO ratio(desorption charge of reductively adsorbed CO_(2) normalized by COad-stripping charge)depend on surface *** particular,QCO_(2)/QCO ratio change better reflects the roughness on an atomic scale,and PZTC is also affected by the electronic modification of Pt atoms in surface *** this study,a comparative study is presented to find a relationship between surface structure and electrochemical properties,which reveals that surface roughness plays a critical role to improve the electrochemical performance of Pt-Ni alloy catalysts with Pt-rich surfaces.

关键词： electrocatalyst fuel cell oxygen reduction reaction Pt-Ni surface roughness

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Skin bioprinting:the future of burn wound reconstruction?

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Burns & Trauma 2019年第1期7卷 25-36页

作者： Mathew Varkey Dafydd O.Visscher Paul P.Mvan Zuijlen Anthony Atala james j.yoo Wake Forest Institute for Regenerative Medicine Wake Forest School of MedicineMedical Center BoulevardWinston-SalemNC 27101USA Department of Plastic Reconstructive and Hand SurgeryAmsterdam University Medical Center1081HVAmsterdamThe Netherlands Amsterdam Movement Sciences AmsterdamThe Netherlands Burn Center Red Cross Hospital1942LEBeverwijkThe Netherlands Association of Dutch Burn Centres 1942LEBeverwijkThe Netherlands

Burns are a significant cause of trauma,and over the years,the focus of patient care has shifted from just survival to facilitation of improved functional ***,burn treatment,especially in the case of extensive burn injuries,involves surgical excision of injured skin and reconstruction of the burn injury with the aid of skin *** skin substitutes do not contain all skin cell types and do not facilitate recapitulation of native skin ***-dimensional(3D)bioprinting for reconstruction of burn injuries involves layer-by-layer deposition of cells along with scaffolding materials over the injured *** bioprinting can be done either in situ or in *** these approaches are similar except for the site of printing and tissue *** are technological and regulatory challenges that need to be overcome for clinical translation of bioprinted skin for burn ***,the use of bioprinting for skin reconstruction following burns is promising;bioprinting will enable accurate placement of cell types and precise and reproducible fabrication of constructs to replace the injured or damaged ***,3D bioprinting is a very transformative technology,and its use for wound reconstruction will lead to a paradigm shift in patient *** this review,we aim to introduce bioprinting,the different stages involved,in vitro and in vivo skin bioprinting,and the various clinical and regulatory challenges in adoption of this technology.

关键词： Bioprinting Skin Burns Reconstruction

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Bioreactor design and validation for manufacturing strategies in tissue engineering

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Bio-Design and Manufacturing 2022年第1期5卷 43-63页

作者： Diana Lim Eric S.Renteria Drake S.Sime Young Min ju ji Hyun Kim Tracy Criswell Thomas D.Shupe Anthony Atala Frank C.Marini Metin N.Gurcan Shay Soker joshua Hunsberger james j.yoo Wake Forest Institute for Regenerative Medicine Wake Forest School of MedicineWinston-SalemNC 27157USA Center for Biomedical Informatics Wake Forest School of MedicineWinston-SalemNC 27157USA RegenMed Development Organization(ReMDO) Winston SalemNC 27106USA

The fields of regenerative medicine and tissue engineering offer new therapeutic options to restore,maintain or improve tissue function following disease or *** maximize the biological function of a tissue-engineered clinical product,specific conditions must be maintained within a bioreactor to allow the maturation of the product in preparation for ***,the bioreactor should be designed to mimic the mechanical,electrochemical and biochemical environment that the product will be exposed to in ***-time monitoring of the functional capacity of tissue-engineered products during manufacturing is a critical component of the quality management *** present review provides a brief overview of bioreactor engineering *** addition,strategies for bioreactor automation,in-line product monitoring and quality assurance are discussed.

关键词： Bioreactor Monitoring sensors and automation Manufacturing Tissue engineering Regenerative medicine

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基于胰腺神经内分泌肿瘤病人术前MRI特征预测术后复发的研究

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国际医学放射学杂志 2022年第3期 370-371页

作者： S.Han j.H.Kim j.yoo S.jang 于子川

摘要目的探讨胰腺神经内分泌肿瘤(PNET)的MRI特征对预测术后复发及其时机的价值。方法回顾性研究2000—2018年间99例接受MRI检查并进行手术的PNET

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静脉内LI-RADS肿瘤的CT和肝胆MRI

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国际医学放射学杂志 2022年第2期 229-230页

作者： j.S.Bae j.M.Lee S.K.jeon j.yoo S.j.Park j.H.yoon 严莹

摘要 CT和/或MRI对静脉内肿瘤肝脏影像报告和数据系统（LR-TIV）分类的诊断性能尚未得到评价。目的以病理结果为参考标准，评估LR-TIV分类对CT和肝胆对比剂

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组织工程中肾脏血管化构建的领先方法（英文）

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Engineering 2022年第12期 117-127+6页

作者： Diana S.Lim john D.jackson Anthony Atala james j.yoo Wake Forest Institute for Regenerative Medicine Wake Forest University School of MedicineMedical Center Boulevard

由于肾衰竭发病率升高的速度远超治疗方法的进展速度,因而对这种疾病新的治疗方法的需求也是以前所未有的速度不断增加。目前的治疗受制于可供应的活器官,而世界范围内均缺乏这种器官。这些治疗模式还需要大量的基础结构,这也极大地限...

由于肾衰竭发病率升高的速度远超治疗方法的进展速度,因而对这种疾病新的治疗方法的需求也是以前所未有的速度不断增加。目前的治疗受制于可供应的活器官,而世界范围内均缺乏这种器官。这些治疗模式还需要大量的基础结构,这也极大地限制了大多数国家的患者获得医疗服务的机会。通过肾脏组织工程方法有望开发出替代的解决方案,解决目前治疗中仍存在的许多不足之处。尽管在生物制造和全器官组织工程方面已经取得了许多进展——主要是在过去的十年中取得的,但仍然存在许多挑战。当前组织工程方法进展中的一个主要障碍是如何对已形成的工程化组织构建体实施成功的血管化。本文主要涉及近年来解决血管问题取得的进展,包括脉管系统的生物制造、通过脱细胞和再细胞化方法实施全器官工程、微尺度器官形成,以及在肾脏组织工程背景下以类器官取得血管化。我们还着重探讨了在制定成功的临床转化策略中仍然存在的具体问题。

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