检索结果-南通市图书馆

An improved CRISPRi system in Pichia pastoris

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2023年第3期8卷 479-485页

作者： Shujing Qiao Fan Bai Peng Cai Yongjin j.Zhou Lun Yao Division of Biotechnology Dalian Institute of Chemical PhysicsChinese Academy of Sciences457 Zhongshan RoadDalian116023PR China CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023PR China Dalian Key Laboratory of Energy Biotechnology Dalian Institute of Chemical PhysicsCAS457 Zhongshan RoadDalian116023PR China University of Chinese Academy of Sciences Beijing100049China

CRISPR interference(CRISPRi)has been developed and widely used for gene repression in various *** we report an improved CRISPRi system in Pichia pastoris by fusing dCas9 with endogenous transcriptional repressor *** CRISPRi system shows strong repression of eGFP,with the highest efficiency of 85%.Repression of native genes is demonstrated by targeting AOX1 ***1 is efficiently repressed and the mutant strains show much slower growth in methanol *** of gRNA expression and processing on CRISPRi efficiency is also *** is found that gRNA processing by HH/HDV ribozymes or Csy4 endoribonuclease generating clean gRNA is critical to achieve strong repression,and Csy4 cleavage shows higher repression ***,gRNA expression using native tRNA transcription and processing systems results in relatively weaker repression of *** expression of two gRNAs targeting promoters of eGFP and AOX1 in an array together with Cys4 recognition sites,both genes can be repressed ***4-mediated gRNA array processing is further applied to repress fatty acyl-CoA synthetase genes(FAA1 and FAA2).Both genes are efficiently repressed,demonstrating that Cys4 endoribonuclease has the ability to cleave gRNAs array and can be can be used for multiplexed gene repression in ***.

关键词： Pichia pastoris CRISPRi HH/HDV Csy4 gRNA array

Hyperparameter Optimization Based Deep Belief Network for Clean Buses Using Solar Energy Model

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Intelligent Automation & Soft Computing 2023年第7期37卷 1091-1109页

作者： Shekaina justin Wafaa Saleh Tasneem Al Ghamdi j.Shermina College of Engineering Princess Nourah Bint Abdulrahman UniversityRiyadh 84428Kingdom of Saudi Arabia Transport Engineering School of Engineering and The Built Environment Edinburgh Napier UniversityEdinburgh EH105DUK Department of Computing Muscat CollegeUniversity of SterlingUK

Renewable energy has become a solution to the world’s energy concerns in recent ***(PV)technology is the fastest technique to convert solar radiation into ***-powered buses,metros,and cars use PV *** technologies are always *** in the parameters that need to be analysed and examined include PV capabilities,vehicle power requirements,utility patterns,acceleration and deceleration rates,and storage module type and capacity,among *** is intermit-tent and *** forecasting and modelling of PV sys-tem output power are key to managing storage,delivery,and smart *** unparalleled data granularity,a data-driven system could better anticipate solar *** learning(DL)models have gained popularity due to their capacity to handle complex datasets and increase computing *** article introduces the Galactic Swarm Optimization with Deep Belief Network(GSODBN-PPGF)*** GSODBN-PPGF model predicts PV power *** GSODBN-PPGF model normalises data using data *** is used to forecast PV power *** GSO algorithm boosts the DBN model’s predicted ***-PPGF projected 0.002 after 40 h but observed *** GSODBN-PPGF model validation is compared to existing *** showed that the GSODBN-PPGF model outperformed recent *** shows that the proposed model is better at forecasting than other models and can be used to predict the PV power output for the next day.

关键词： Photovoltaic systems solar energy power generation prediction model deep learning

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

明天的世界

明天的世界

EFFECT OF THE HEAT OF REACTION DURING THE ALUMINOTHERMIC PROCESS OF ILMENITE

作者： j.·莫菲(j. Morphy)原

来源：南通市图书馆图书评论

学校读者我要写书评

暂无评论

Acta Metallurgica Sinica(English Letters) 2005年第2期18卷 173-176页

作者： Y.Z.Lan j.Liu R.W.Smith MaterialsDepartment YunnanUniversityKunming650091China MetallurgicalandMaterialsDepartment NevadaUniversityofRenoNV89503NevadaUSA

The influence of the heat of reaction during the aluminothermic process for the production of ferrotitanium was investigated. The effect of the variation of the heat of reaction on FeTi and Ti recovery and the topographical analysis of slag produced showed that FeTi and Ti recovery increased and Al content in the alloy decreased with the increase of the absolute value of ΔH when │ΔH│j/kg; however, when │ΔH│>2584.24K·j/kg, FeTi and Ti recovery decreased with an increase of the absolute value of ΔH. Therefore, the heat of reaction in the aluminothermic process of ilmenite should be strictly controlled to -2584.24K·j/kg.

关键词： ilmenite Al reduction the heat of reaction

维普期刊数据库

Urethane Modified Hydrophobic Compact Wood Pulp Paper for Oil Spill Cleanup: A Preliminary Study

在线全文

学校读者我要写书评

暂无评论

journal of Renewable Materials 2020年第10期8卷 1257-1268页

作者： Gustavo de Souza Ricardo K.Kramer Antonio j.F.Carvalho Department of Materials Engineering Sao Carlos School of Engineering(EESC)University of São Paulo(USP)São PauloBrazil

Oil spills and oil/water wastewater are among the great concerns regarding oil *** technologies face many limitations and in some cases are responsible for causing secondary pollution,therefore there is as seek for environmental friendly ***,from which celluloses are highlighted,are being employed for oil/water separation or oil absorbents ***,these membranes are obtained by freeze drying of CNF(cellulose nano-fibrils)suspensions followed by chemical modification for hydrophobization,which involves expensive process as chemical vapor deposition and expensive reactants as sylanes,turning these processes hardly ***,we produced a natural porous structure paper from eucalyptus pulp fibers modified by a dipping and heating process in a blocked diisocyanate *** the surface treatment,contact angle with water reached 144°and water absorption reduced seven times,keeping the good oil *** chemical modification process is simple to be performed and use a very low quantity of reactant estimated to be less than 0.1 wt%based on *** good mechanical properties of the material allows its use in non usual conditions which can be of great importance depending on the environmental conditions.

关键词： Cellulose oil removal hydrophobization water repellency blocked isocyanates

Phase Control of Transient Optical Properties of Double Coupled Quantum-Dot Nanostructure via Gaussian Laser Beams

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Physics Letters 2018年第2期35卷 29-33页

作者： j.Shiri F.Shahi M.R.Mehmannavaz L.Shahrassai Young Researchers and Elite Club Tabriz BranchIslamic Azad University Department of Atomic and Molecular Physics University of Tabriz Young Researchers and Elite Club Mashhad BranchIslamic Azad University

We theoretically analyze the transient properties of a probe field absorption and dispersion in a coupled semiconductor double-quantum-dot *** show that in the presence of the Gaussian laser beams,absorption and dispersion of the probe field can be dramatically influenced by the relative phase between applied fields and intensity of the Gaussian laser *** and steady-state behaviors of the probe field absorption and dispersion are discussed to estimate the required switching *** estimated range is between 5-8 ps for subluminal to superluminal light propagation.

关键词： Phase Control of Transient Optical Properties of Double Coupled Quantum-Dot Nanostructure via Gaussian Laser Beams QDs

维普期刊数据库

The use of electro-deoxidation in molten salts to reduce the energy consumption of solar grade silicon and increase the output of PV solar cells

在线全文

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2015年第6期25卷 583-590页

作者： Paul R.Coxon Michael Coto Eimutis juzeliunas Derek j.Fray Department of Materials Science & Metallurgy University of Cambridge Institute of Chemistry Centre for Physical Sciences & Technology 3. Klaip?da University

Solar photovoltaics,based upon silicon,are the most popular form of solar cell with efficiencies around 20%.These efficiencies can be further increased by employing light trapping schemes to minimise optical losses through scattering and reflection which enhances the amount of light absorbed and number of photo-carriers *** approaches employ antireflection coatings（ARCs） or texturise the surface of the silicon disks,so that the structure consists of an array of needles which can absorb most of the ***,these structures are created by leaching the silicon with hydrofluoric-based acids or by reactive ion etching（RIE） *** paper reviews some of the methods for improving the energy efficiency of silicon production,and describes the use of electro-deoxidation of SiO;layers,on silicon,in molten calcium chloride to form nanoporous black silicon（b-Si） *** coating b-Si surface with TiO;,a common ARC,extremely black surfaces with negligible reflectance of about 0.1%,are produced,which can have applications for low-cost high efficiency solar cells.

关键词： Black silicon Solar cell photovoltaics Silicon molten salt Electrochemistry

Does sexual dimorphism vary by population? Laryngeal and ear anatomy in cricket frogs

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Current Zoology 2019年第3期65卷 343-352页

作者： Blinda E.MCCLELLAND Michael j.RYAN Walter WILCZYNSKI Department of Integrative Biology University of Texas at Austin Austin TX 78712 USA Neuroscience Institute and Center for Behavioral Neuroscience Georgia State University Atlanta GA 30303 USA

Acoustic communication in many anuran species can show the effects of both natural and sexual selection. This is reflected in the sexually dimorphic an atomy of the lary nx and ear structures, as well as the allometric relationship of these morphological traits to head or body size. In this study, we examined laryngeal and ear structures of cricket frogs Acris crepitans not only as sexually dimorphic characteristics, but also as they differ across populations in environmentally different habitats. We used 2-way ANOVA to determine whether the volumetric or linear measurements of these structures differed by sex and population. Females have significantly larger body, head, and ear sizes, but significantly smaller larynges than males. Furthermore, females as well as males show larger body and head sizes, ears, and larynges in a dryer open habitat. An ANCOVA analysis shows that males, but not females, differ in laryngeal size across populations beyond the allometric changes attributable to head size alone indicating that males have a greater degree of laryngeal population variation. In contrast, our covariate analysis found that in both sexes many of the ear differences are non-sigrdficant once head size is accounted for, suggesting that most of the population-level ear variation is due to allometric effects of body size. We conclude that although both sexes show size differences in the larynx related to selection for larger body size in dry, open habitats, selection on males for larger larynx size related to the production of lower frequency calls in those habitats does not result in correlated changes in the female larynx. The results suggest that in anurans, selection for changes in body and head size affects both sexes equally, male calls and the vocal structures responsible for them can further diversify without concordant changes in females.

关键词： acoustic communication allometry amphibians population variation sex differences

Magnesium:pathophysiological mechanisms and potential therapeutic roles in intracerebral hemorrhage

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2019年第7期14卷 1116-1121页

作者： jason j.Chang Rocco Armonda Nitin Goyal Adam S.Arthur Department of Critical Care Medicine MedStar Washington Hospital Center Department of Neurology Georgetown University School of Medicine Department of Neurosurgery Georgetown University School of Medicine Department of Neurology University of Tennessee Health Science Center Semmes Murphey Clinic Department of Neurosurgery University of Tennessee Health Science Center

Intracerebral hemorrhage(ICH) remains the second-most common form of stroke with high morbidity and *** can be divided into two pathophysiological stages:an acute primary phase,including hematoma volume expansion,and a subacute secondary phase consisting of blood-brain barrier disruption and perihematomal edema *** date,all major trials for ICH have targeted the primary phase with therapies designed to reduce hematoma expansion through blood pressure control,surgical evacuation,and ***,none of these trials has resulted in improved clinical *** is a ubiquitous element that also plays roles in vasodilation,hemostasis,and blood-brain barrier *** models have highlighted potential therapeutic roles for magnesium in neurological diseases specifically targeting these pathophysiological *** studies have also demonstrated inverse associations between admission magnesium levels and hematoma volume,hematoma expansion,and clinical outcome in patients with *** associations,coupled with the multifactorial role of magnesium that targets both primary and secondary phases of ICH,suggest that magnesium may be a viable target of study in future ICH studies.

关键词： intracerebral hemorrhage stroke,magnesium vasodilation hemostasis blood-brain barrier perihematomal edema

维普期刊数据库