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replication of natural surface topographies to generate advanced cell culture substrates

Bioactive materials 2023年第10期28卷 337-347页

作者： N.O.monteiro j.F.Fangueiro r.l.reis N.m.Neves 3B’s research Group I3Bs-Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAveParkParque de Ciencia e Tecnologi1Zona Industrial da Gandr14805-017BarcoGuimaraesPortugal ICVS/3B’s-PT Government Associate laboratory Brag1Guimar˜aesPortugal

Surface topographies of cell culture substrates can be used to generate in vitro cell culture environments similar to the in vivo cell niches. In vivo, the physical properties of the extracellular matrix (ECm), such as its topography, provide physical cues that play an important role in modulating cell function. mimicking these properties remains a challenge to provide in vitro realistic environments for cells. Artificially generated substrates’ topographies were used extensively to explore this important surface cue. more recently, the replication of natural surface topographies has been enabling to exploration of characteristics such as hierarchy and size scales relevant for cells as advanced biomimetic substrates. These substrates offer more realistic and mimetic environments regarding the topographies found in vivo. This review will highlight the use of natural surface topographies as a template to generate substrates for in-vitro cell culture. This review starts with an analysis of the main cell functions that can be regulated by the substrate’s surface topography through cell-substrate interactions. Then, we will discuss research works wherein substrates for cell biology decorated with natural surface topographies were used and investigated regarding their influence on cellular performance. At the end of this review, we will highlight the advantages and challenges of the use of natural surface topographies as a template for the generation of advanced substrates for cell culture.

关键词： Biomimetic topography Cell culture substrates Natural surface topographies

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Small extrachromosomal circular DNA in amyotrophic lateral sclerosis matter

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Neural regeneration research 2025年第5期20卷 1411-1413页

作者： marcos j.Arauzo-Bravo Daniela Gerovska matthias Schwab Alexandra Kretz Computational Biology and Systems Biomedicine Biodonostia Health Research InstituteSan SebastianSpain Basque Foundation for Science IKERBASQUEBilbaoSpainMax Planck Institute for Molecular BiomedicineComputational Biology and Bioinformatics GroupMünsterNorth Rhine-WestphaliaGermany Department of Cell Biology and Histology Faculty of Medicine and NursingUniversity of Basque Country(UPV/EHU)LeioaSpain Department of Neurology Jena University HospitalJenaThuringiaGermany

Comprehensive studies identify motor neuron spectrum disorders including amyotrophic lateral sclerosis(AlS)as globally rising fatal disorders with the highest prevalence in aging populations,influenced by ethnicity and ancestry(GBD 2016 motor Neuron Disease Colla borators,2018).While~10% of diagnoses involve a family history(fAlS),most cases are considered sporadic(sAlS).However,population-based studies suggest that even cases without a common index mutation impart heritability(ryan et al.,2019),indicating a crucial role of rare and as yet unknown genetic denominators.

关键词： amyotrophic sclerosis

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Assessment of mercury(II) bioavailability using a bioluminescent bacterial biosensor: Practical and theoretical challenges

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journal of Environmental Sciences 2010年第8期22卷 1137-1143页

作者： Paulo r G Barrocas William m landing robert j m Hudson Department of Sanitation and Environmental Health National School of Public Health Oswaldo Cruz Foundation Department of Oceanography Florida State University. Tallahassee Department of Natural resources and Environmental Sciences University of Illinois

Critical methodological challenges in the microbial biosensor approach to assessing Hg（II） bioavailability were evaluated from the perspective of analytical chemists. The main challenge stems from the fact that the chemical speciation of Hg（II） in natural waters exerts a major control on its bioavailability, yet its natural complexation equilibria are extensively altered during conventional bioassays. New data, obtained using a bioluminescent Hg（II）-biosensor, that illustrate these challenges are presented and potential solutions proposed.

关键词： mercury bioavailability biosensor chemical speciation bioluminescent

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Atrazine removal from water by activated charcoal cloths

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International Soil and Water Conservation research 2020年第2期8卷 205-212页

作者： javier m.Gonzalez lynnette r.murphy Chad j.Penn Veera m.Boddu laura l.Sanders National Soil Erosion research laboratory USDA-ARSWest LafayetteIN47907USA Earth Science Department Northeastern Illinois UniversityChicagoIL60625USA O’Neill School of Public and Environmental Affairs University of IndianaBloomingtonIN47405USA Plant Polymer research Unit National Center for Agriculture Utilization ResearchUSDA-ARSPeoriaIL61604USA

Pollution control structures may be adapted to enhance the removal of nutrients and pesticides from water. Charcoal-like material is known to sorb organic compounds in solution, including atrazine, a herbicide used to control broadleaf weeds, which persists in soils and can migrate from soils to water bodies, ultimately affecting water quality. The primary goal of this study was to investigate if activated charcoal cloths (single-weave, SW;double-weave, DW;and knitted, KT) are more efficient to remove atrazine from aqueous solutions than a cloth without activated charcoal (control) currently used in pollution control structures. The approach consisted of sorption kinetics, flow-through, and desorption/degradation experiments using 50 and 1000μg l-1 atrazine solutions. results showed that within 30 min of contact time, the control sorbed from 22.6 to 36.1% of atrazine in solution;whereas the activated charcoal cloths sorbed from 76 to 99% of atrazine in solution (SW > KT > DW). The flow-through ex-periments showed that the SW sorbed 88.2, 76.1, and 52.2%of atrazine at the contact time of 0.75, 5, and 10 min, respectively. After 28 days of incubation, previously sorbed atrazine on the SW cloth did not degrade and rbed. The results showed that activated charcoal cloths are a practical alternative for improving atrazine removal in water in pollution control structures.

关键词： Atrazine Activated charcoal Water quality Pesticide Cloth

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Nonlinear elastic constitutive relations of residually stressed composites with stiff curved fibres

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Applied mathematics and mechanics(English Edition) 2022年第10期43卷 1515-1530页

作者： m.H.B.m.Shariff j.merodio r.Bustamante Department of mathematics Khalifa University of Science and TechnologyAbu Dhabi127788United Arab Emirates Departamento de matemática Aplicada a las TIC ETS de Ingeniería de Sistemas InformaticosUniversidad Politecnica de MadridMadrid28031Spain Departamento de Ingeniería mecánica Universidad de ChileBeauchef 851Santiago CentroSantiago8370448Chile

mechanical models of residually stressed fibre-reinforced solids,which do not resist bending,have been developed in the ***,in some residually stressed fibre-reinforced elastic solids,resistance to fibre bending is significant,and the mechanical behavior of such solids should be ***,in this paper,we model the mechanical aspect of residually stressed elastic solids with bending stiffness due to fibre curvature,which up to the authors’knowledge has not been mechanically modeled in the *** proposed constitutive equation involves a nonsymmetric stress and a couple-stress *** invariants are used in the constitutive equation,where each spectral invariant has an intelligible physical meaning,and hence they are useful in experiment and analysis.A prototype strain energy function is ***,we use this prototype to give results for some cylindrical boundary value problems.

关键词： fibre-reinforced solid residual stress bending stiffness spectral invariant second gradient finite elasticity

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lattice expansion and smaller CuO_(x)CeO_(2-δ)particles formation by magnesium interaction for low temperature CO oxidation

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journal of rare Earths 2021年第4期39卷 434-439页

作者： j.m.Waikar r.K.more N.r.lavande P.m.more Department of Chemistry Institute of Chemical TechnologyMatungaMumbai400019India

A series of magnesia doped CuOxCeO_(2-δ)catalysts were prepared by co-precipitation followed by impregnation method and investigated for CO *** manuscript is devoted to explaining the role of mgO for the formation of active species on the CuOxCeO_(2-δ)*** improvement in catalytic activity is ascribed to the formation of various active species due to the interaction of magnesia with CuOxCeO_(2-δ).The catalysts were characterized by PXrD,N_(2)adsorption,H_(2)-TPr,XPS,SEm,EDS and HrTEm *** mg doped catalyst shows lattice expansion of ceria due to the formation of smaller Ce^(3+)species with oxygen *** mg-O bond also takes part in CO activation and oxidation,which results in the increase of CO *** mg doped CuOxCeO_(2-δ)catalyst shows improvement in low temperature activity compared with the CuOxCeO_(2-δ).

关键词： Adsorbed oxygen lattice expansion low temperature oxidation Ce^(3+) Cu^(+) rare earths

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Science missions Using CubeSats

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空间科学学报 2020年第4期40卷 443-461页

作者： SEYEDABADI m E FAlANGA m AZAm m BArESI N FlÉrON r jANTArACHOTE V jUArEZ OrTIZ V A jUlca YAYA j j lANGEr m mANUTHASNA S mArTINOD N mUGHAl m r NOmAN m PArK j PImNOO A PrAKS j rEYNErI l SANNA A ŞIŞmAN TÇ SOmE j UlAmBAYAr T YU Xiaozhou DONG Xiaolong BAlDIS l Asia-Pacific Space Cooperation Organization 100070 BeijingChina International Space Science Institute-Beijing 100190 BeijingChina International Space Science Institute 3012 BernSwitzerland Bangladesh Space research and remote Sensing Organization 1207 DhakaBangladesh japan Aerospace Exploration Agency Institute of Space Astronautical Science252-5210 TokyoJapan Technical University of Denmark 2800 LyngbyDenmark Department of Electrical Engineering Faculty of EngineeringPrince of Songkla University90112 SongkhlaThailand National Commission of Aerospace research and Development 15046 San IsidroPeru Technical University of munich 80333 MunichGermany mahanakorn University of Technology 10530 BangkokThailand Swiss Federal Institute of Technology lausanne 1015 LausanneSwitzerland Institute of Space Technology 44000 IslamabadPakistan Korea Astronomy and Space Science Institute 34055 DaejeonSouth Korea Geo-Informatics and Space Technology Development Agency 10210 BangkokThailand Department of Electronics and Nanoengineering Aalto University02150 EspooFinland Department of Electronics and Telecommunications Polytechnic of Turin10129 TurinItaly Department of Physics University of Cagliari09042 MonserratoItaly Department of Astronautical Engineering University of Turkish Aeronautical Association06790 AnkaraTurkey Nanosatellite Development laboratory National University of Mongolia210646 UlaanbaatarMongolia Dalian University of Technology 116024 DalianChina National Space Science Center 100190 BeijingChina

As the role of missions and experiments carried out in outer space becomes more and more essential in our understanding of many earthly problems,such as resource management,environmental problems,and disaster management,as well as space science questions,thanks to their lower cost and faster development process CubeSats can benefit humanity and therefore,young scientists and engineers have been motivated to research and develop new CubeSat *** very long after their inception,CubeSats have evolved to become accepted platforms for scientific and commercial *** last couple of years showed that they are a feasible tool for conducting scientific experiments,not only in the Earth orbit but also in the interplanetary *** many countries,a CubeSat mission could prompt the community and young teams around the world to build the national capacity to launch and operate national space *** paper presents an overview of the key scientific and engineering gateways opened up to the younger scientific community by the advent and adaptation of new technology into CubeSat *** role of cooperation and the opportunities for capacity-building and education are also ***,the present article also aims to provide useful recommendations to scientists,early-career researchers,engineers,students,and anyone who intends to explore the potential and opportunities offered by CubeSats and CubeSats-based missions.

关键词： CubeSat Space science Small satellites for space science Education Earth observation

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Prediction of strength and deformability of an interlocked blocky rock mass using UDEC

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journal of rock mechanics and Geotechnical Engineering 2017年第3期9卷 149-160页

作者： Y.m.Alshkane A.m.marshall l.r.Stace Department of Civil Engineering University of Sulaimani Sulaimani Kurdistan Region Iraq Department of Civil Engineering University of Nottingham University Park Nottingham NG7 2RD UK

The accurate prediction of strength and deformability characteristics of rock mass is a challenging *** practice,properties of a rock mass are often estimated from available empirical relationships based on the uniaxial compressive strength(UCS).However,UCS does not always give a good indication of in situ rock mass strength and *** aim of this paper is to present a methodology to predict the strength and deformability of a jointed rock mass using UDEC(universal distinct element code).In the study,the rock mass is modelled as an assemblage of deformable blocks that can yield as an intact material and/or slide along predefined joints within the rock mass.A range of numerical simulations of uniaxial and triaxial tests was conducted on rock mass samples in order to predict the equivalent mechanical properties for the rock mass under different loading ***,results are compared with the deformability parameters obtained by analytical methods.

关键词： Strengt Deformability ock mass Universal distinct element code(UDEC)

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Impact of land Degradation on Soil microbial Biomass and Activity in Northeast Brazil

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Pedosphere 2012年第1期22卷 88-95页

作者： j.S.NUNES A.S.F.ArAUjO l.A.P.l.NUNES l.m.lImA r.F.V.carNEIrO A.A.C.SAlVIANO S.m.TSAI Soil Quality lab. Agricultural Science CenterFederal University of PiauíTeresinaPI(Brazil) Center of Nuclear Energy in Agriculture University of Sao PauloPiracicabaSP(Brazil)

land degradation causes great changes in the soil biological *** process of degradation may decrease soil microbial biomass and consequently decrease soil microbial *** study was conducted out during 2009 and 2010 at the four sites of land under native vegetation(NV),moderately degraded land(lDl),highly degraded land(HDl) and land under restoration for four years(rl) to evaluate changes in soil microbial biomass and activity in lands with different degradation levels in comparison with both land under native vegetation and land under restoration in Northeast *** samples were collected at 0-10 cm *** organic carbon(SOC),soil microbial biomass C(mBC) and N(mBN),soil respiration(Sr),and hydrolysis of fluorescein diacetate(FDA) and dehydrogenase(DHA) activities were *** two years of evaluation,soil mBC,mBN,FDA and DHA had higher values in the NV,followed by the *** decreases of soil microbial biomass and enzyme activities in the degraded lands were approximately 8-10 times as large as those found in the ***,after land restoration,the mBC and mBN increased approximately 5-fold and 2-fold,respectively,compared with the *** results showed that land degradation produced a strong decrease in soil microbial ***,land restoration may promote short-and long-term increases in soil microbial biomass.

关键词： land restoration microorganisms soil quality soil respiration

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Down-and up-conversion processes in Nd^(3+)/Yb^(3+)co-doped sodium calcium silicate glasses with concomitant Yb^(2+)assessment

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journal of rare Earths 2023年第3期41卷 342-348,I0001页

作者： r.F.muniz V.S.Zanuto m.S.Gibin j.V.Gunha A.Novatski j.H.rohling A.N.medina m.l.Baesso Departament of Science State University of MaringáAv.Reitor Zeferino Vaz s/nGoioerê87360-000PRBrazil Departament of Physics State University of MaringáAv.Colombo 5790Maringá87020-900PRBrazil Departament of Physics State University of Ponta GrossaAv.Carlos Cavalcanti4748Ponta Grossa 84030-900PRBrazil

Novel Nd^(3+)/Yb^(3+)co-doped sodium calcium silicate glasses were prepared by melting quenching method:Spectroscopic study was carried out as a function of doping content by fixing sensitizer(Nd^(3+))concentration to 0.2 mol%and adjusting activator(Yb^(3+))from 0 to 1.0 mol%.The energy transfer(ET)mechanisms between Nd^(3+)and Yb^(3+)are discussed based on their energy levels and excitation powerdependence emission *** show that the presence of Yb^(2+)might be considered for the Nd^(3+)-free and co-doped *** ET was confirmed by the down-conversion NIr emission spectra of the doped and co-doped samples under excitation at 808 *** mechanisms observed seem to involve only one VIS absorbed photon for each NIr emitted via direct energy transfer between^(4)F_(3/2)of Nd^(2+)and^(2)F_(5/2)of Yb^(3+)compensated by phonon assistance due to energy gap between these *** efficiency of ET increases with the ytterbium content up to almost 90%for the sample with 1 mol%of Yb2O3,which was evaluated by lifetime ***-conversion photoluminescence by exciting trivalent ions of neodymium(808 nm)and ytterbium(975 nm)is *** observed up-converted emission bands are related to the 4f-4f transitions of Nd^(3+)and the spin-forbidden 5d-4f transition of Yb^(2+).Nd^(3+)upconversion emission is observed under 975 nm excitation,presenting an almost quadratic emission dependence with power excitation,which suggests that two laser photons participate in the up-conversion(UC)process,showing that ET occurs by a phonon-assisted energy transfer and cooperative energy transfer.

关键词： Solar cells Energy transfer Glassy systems rare earths

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