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Single-cell omics: experimental workflow, data analyses and applications

Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin Dong Bin Duan Feiyang Xing jun Wu Minmin Xiao Fangqing Zhao jing-Dong j.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

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Safe mining practices under wide spans in underground non-caving mines——Case studies

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International journal of Mining Science and Technology 2019年第4期29卷 535-540页

作者： j.j.Ran Kinross Gold Corporation

Mining under wide span is of concern as it increases the probability of back caving causing personnel injury and equipment damage in underground mines in open stoping and underhand drift-and-fill *** restricting personnel access to well supported lateral development is commonly practiced,it is not always possible to implement this requirement due to various factors such as ore loss control,drilling equipment limitations,availability of remote operating capacity and consideration of *** with rules implemented to limit personnel entry into openings with wide spans,the hazards of equipment damage and back caving still *** the years,different practices have been reported and adopted to minimize risks associated with exposure to large spans in various underground *** from these practices are beneficial to current and future mines with challenges of safe extraction of thick deposits in a non-caving *** paper briefly summarizes practices in mining wide orebodies using the open stoping method without personnel access and underhand mining using the drift-andfill method with personnel exposure in the industry and presents cases from Kinross mines where the hanging pillar design was tested,and stope backs were naturally and artificially supported for extraction under wide spans using the open stoping method.

关键词： Mine safety Wide span Back stability Underhand mining Hanging pillar

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Gas Line Pressure Fluctuation Analysis of a Gas-Liquid Reactor

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journal of Thermal Science 2005年第3期14卷 267-271页

作者： j.j.j. CHEN j.C. ZHAO Chemical & Materials Engineering Department The University of Auckland Private Bag 92019 Auckland New Zealand ProfessorChemical & Materials Engineering Department The University of Auckland Private Bag 92019 Auckland New Zealand

To ensure efficient operation of metallurgical gas-liquid reactors, the gas bubbles must be uniformly distributed. For high temperature metallurgical reactors, it is impractical and unsafe to carry out visual observations. An air-water model was used to study the relationship between the bubble flow patterns and the pressure fluctuation signals. The fluctuation signals captured in the time domain were transformed into the frequency domain. Various parameters obtained from the transformed data were analysed for their suitability for delineating the bubble flow patterns observed. These parameters and the flow patterns were found to be well-correlated using the gas flow number.

关键词： gas liquid reactors bubble distribution signal analysis

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Computational study on influence of RF shielding box on inductance of coil

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Plasma Science and Technology 2019年第6期21卷 23-28页

作者： S j KIM j j LEE Y S LEE j H KIM S j YOU Applied Physics lab for PLasma Engineering(APPLE) Department of Physics Chungnam National University Daejeon 34134 Republic of Korea Korea Research Institute of Standards and Science(KRISS) Daejeon 34113 Republic of Korea

Despite the fact that a radio frequency (RF) shielding box affects a coil inductance used in matching network,RF engineers have used a coil inductance measured in open space on designing matching networks since it is difficult to precisely measure the coil inductance within the RF shielding *** this work,we investigate the influences of the RF shielding box on the coil inductance via a 3D full electromagnetic wave *** results shows that the coil inductance decreases from-6.0% to-11.9% compared with its ideal inductance depending on coil positions within the RF shielding *** inductive and capacitive coupling between the coil and surfaces of the RF shielding box contribute to the reduction of the coil *** expect that these results would be useful for those who design RF matching networks.

关键词： 3D full EM wave simulation RF matching component RF shield inductive and capacitive coupling

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Formation of High-quality Advanced High-k Oxide Layers at Low Temperature by Excimer UV Lamp-assisted Photo-CVD and Sol-gel Processing

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Chemical Research in Chinese Universities 2004年第4期20卷 396-402页

作者： YU j. j. Electronic & Electrical Engineering University College London

We have successfully demonstrated that high quality and high dielectric constant layers can be fabricated by low temperature photo-induced or -assisted processing. Ta_2O_5 and ZrO_2 have been deposited at t<400 ℃ by means of a UV photo-CVD technique and HfO_2 by photo-assisted sol-gel processing with the aid of excimer lamps. The UV annealing of as-grown layers was found to significantly improve their electrical properties. Low leakage current densities on the order of 10 -8 A/cm 2 at 1 MV/cm for deposited ultrathin Ta_2O_5 films and ca.10 -6 A/cm 2 for the photo-CVD ZrO_2 layers and photo-irradiated sol-gel HfO_2 layers have been readily achieved. The improvement in the leakage properties of these layers is attributed to the UV-generated active oxygen species O( 1D) which strongly oxidize any suboxides to form more stoichiometric oxides on removing certain defects, oxygen vacancies and impurities present in the as-prepared layers. The photo-CVD Ta_2O_5 films deposited across 10.16-cm Si wafers exhibit a high thickness uniformity with a variation of less than \{±2.0%\} being obtained for ultrathin ca.10 nm thick films. The lamp technology can in principle be extended to larger area wafers, providing a promising low temperature route to the fabrication of a range of high quality thin films for future ULSI technology.

关键词： Excimer lamp High-kdielectrics Thin film Chemical vapour deposition(CVD) Sol-gel processing

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Indian plate paleogeography, subduction and horizontal underthrusting below Tibet: paradoxes, controversies and opportunities

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National Science Review 2022年第8期9卷 175-193页

作者： Douwe j.j.van Hinsbergen Department of Earth Sciences Utrecht University

The India–Asia collision zone is the archetype to calibrate geological responses to continent–continent collision, but hosts a paradox: there is no orogen-wide geological record of oceanic subduction after initial collision around 60–55 Ma, yet thousands of kilometers of post-collisional subduction occurred before the arrival of unsubductable continental lithosphere that currently horizontally underlies Tibet. Kinematically restoring incipient horizontal underthrusting accurately predicts geologically estimated diachronous slab break-off, unlocking the Miocene of Himalaya–Tibet as a natural laboratory for unsubductable lithosphere convergence. Additionally, three endmember paleogeographic scenarios exist with different predictions for the nature of post-collisional subducted lithosphere but each is defended and challenged based on similar data types. This paper attempts at breaking through this impasse by identifying how the three paleogeographic scenarios each challenge paradigms in geodynamics, orogenesis, magmatism or paleogeographic reconstruction and identify opportunities for methodological advances in paleomagnetism, sediment provenance analysis, and seismology to conclusively constrain Greater Indian paleogeography.

关键词： collision orogenesis subduction reconstruction Himalaya Tibet

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"反介入"战役及制胜要素——《反介入战争》第四章(三)

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兵器 2025年第1期 48-54页

作者： [美]萨姆·j·坦格里迪吴志丹(译) 不详

二战英国战役的胜利与纳粹失败的海狮行动被认为是二战转折点的英国战役,研究成果颇为丰硕。在纳粹对不列颠群岛发动空袭前,尽管德国海军在对英国海军和入侵挪威的战斗中不时会有损失,但德国陆军的“闪电战”从未失败过。

关键词：不列颠群岛英国海军研究成果纳粹转折点战役二战

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Application of CCD Cameras to Investigations of Mixing on Boundaries of a Thermal Plasma jet

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Plasma Science and Technology 2007年第6期9卷 743-746页

作者： j.HLINA j.SONSKY j.SLECHTA Institute of Thermomechanics Academy of Sciences of the Czech RepublicDolejskova 5182 00 Praha 8Czech Republic

Mixing of a thermal plasma jet with the surrounding atmosphere was studied using two CCD cameras （PCO SensiCam） situated detecting simultaneously the radiation of argon and nitrogen. The evaluation of image differences between two records showed that the location of regions on plasma jet boundaries characterised by stronger nitrogen radiation changes with the plasma flow rate. Close-to-laminar flow results in a small mixing rate and consequently low nitrogen optical emission on plasma jet boundaries. The increase of the flow rate leads to the formation of a relatively thick and stable layer on the boundaries characterised by strong nitrogen radiation. Further enhancement of the flow rate results in the formation of unstable regions of excited nitrogen molecules moving along the jet.

关键词： thermal plasma jet mixing CCD camera

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Design,fabrication,characterization and reliability study of CMOS-MEMS Lorentz-force magnetometers

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Microsystems & Nanoengineering 2022年第5期8卷 17-41页

作者： j.j.Valle j.M.Sánchez-Chiva D.Fernández j.Madrenas Department of Electronic Engineering Universitat Politècnica de CatalunyaJordi Girona 1 i 3Edifici C408034 BarcelonaSpain Sorbonne Université CNRSLaboratoire de Recherche en Informatique(LIP6)UMR76064 place Jussieu75005 ParisFrance Institut de Física d’Altes Energies(IFAE) The Barcelona Institute of Science and Technology(BIST)Edifici Cn.Facultat Ciències NordUniversitat Autónoma de Barcelona08193 Bellaterra(Barcelona)Spain

This article presents several design techniques to fabricate micro-electro-mechanical systems(MEMS)using standard complementary metal-oxide semiconductor(CMOS)*** were applied to fabricate high yield CMOS-MEMS shielded Lorentz-force magnetometers(LFM).The multilayered metals and oxides of the back-end-of-line(BEOL),normally used for electronic routing,comprise the structural part of the *** most important fabrication challenges,modeling approaches and design solutions are *** that predict the Q factor,sensitivity,Brownian noise and resonant frequency as a function of temperature,gas pressure and design parameters are presented and validated in characterization tests.A number of the fabricated magnetometers were packaged into Quad Flat No-leads(QFN)*** show this process can achieve yields above 95%when the proper design techniques are *** CMOS not being a process for MEMS manufacturing,estimated performance(sensitivity and noise level)is similar or superior to current commercial magnetometers and others built with MEMS ***,typical offsets present in Lorentz-force magnetometers were prevented with a shielding electrode,whose efficiency is ***,several reliability test results are presented,which demonstrate the robustness against high temperatures,magnetic fields and acceleration shocks.

关键词： Lorentz magnetometer characterization

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Bulk and surface damages in complementary bipolar junction transistors produced by high dose irradiation

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Chinese Physics B 2018年第1期27卷 430-437页

作者： j Assaf Atomic Energy Commission Damascus Syria

Two complementary types NPN and PNP of bipolar junction transistors （BjTs） were exposed to higll dose of neutrons and gamma rays. The change in the base and collector currents, minority carriers lifetime, and current gain factor/3 with respect to the dose were analyzed. The contributions of the base current according to the defect types were also reported. It was declared that the radiation effect of neutrons was almost similar between the two transistor types, this effect at high dose may decrease the value of/3 to less than one. The Messenger-Spratt equation was used to describe the experimental results in this case. However, the experimental data demonstrated that the effect of gamma rays was generally higher on NPN than PNP transistors. This is mainly attributed to the difference in the behavior of the trapped positive charges in the SiO2 layers. Meanwhile, this difference tends to be small for high gamma dose.

关键词： bipolar junction transistors radiation effects surface damage bulk damage

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