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The Roles of pH in the Modification of Wild-Type Recombinant Phlebia radiata Manganese Peroxidase 3 Activities and Stability of Secondary Structures

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American journal of Molecular Biology 2022年第4期12卷 158-180页

作者： Usenobong F. Ufot Imeh j. Okop Mfoniso P. Uko Imekan I. Akpan Khasim cali Monday I. Akpanabiatu Department of Biochemistry Faculty of Biological Sciences Akwa Ibom State University Uyo Nigeria Department of Chemistry Faculty of Biological Sciences Akwa Ibom State University Uyo Nigeria Department of Microbiology Faculty of Biological Sciences Akwa Ibom State University Uyo Nigeria Department of Zoology Faculty of Biological Sciences Akwa Ibom State University Uyo Nigeria Department of Chemical Engineering and Analytical Science University of Manchester Manchester UK Department of Biochemistry Faculty of Sciences University of Uyo Uyo Nigeria

This investigation is aimed at understanding the specific role of pH and calcium ions on the activity and stability of wild-type recombinant Phlebia radiata manganese peroxidase 3 (rPr-MnP3). The pH-dependent cycle of reactions for rPr-MnP3 was evaluated by investigating time-dependent changes in the activity and electronic absorption spectrum of rPr-MnP3.The rPr-MnP3 had maximum efficacy (kcat/Km) for Mn (II) oxidation at pH 5.0 and 3.0 for oxidation of ABTS. Raising the pH of a solution of resting rPr-MnP3 from pH 6.7 (form XH) to pH 8.6 (form X−), a rapid alkaline transition occurs. Leaving the X− form of the enzyme at pH 8.6, it slowly becomes converted to a third form of the enzyme Y−, which returned to the original XH form of the enzyme at pH 6.7. Recovery of form XH from form Y− occurred through an intermediate Z form. The pH inactivation of rPr-MnP3 followed first-order kinetics. The rate of formation of XH from Z is pH-dependent and biphasic in nature, with measured rate constants (k) = 0.25 min−1, and half-life (T1/2) = 2.8 min. The pH-dependent properties observed may be indicative of a greater degree of conformational flexibility at rPr-MnP3 active site due to disruption of the haem-linked hydrogen-bonding network in the distal haem pocket. Calcium ions were observed to significantly stabilised the enzyme’s spectral features and reduce the loss of activity during the alkaline pH transition. Calcium ions enhance the recovery of the initial activity but cannot prevent the final time-dependent irreversible denaturation and aggregation.

关键词： Phlebia radiata Manganese Peroxidase pH-Dependence Inactivation Conformation

Energy modeling and data structure framework for Sustainable Human-Building Ecosystems （SHBE）- a review

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Frontiers in Energy 2018年第2期12卷 314-332页

作者： Suraj TALELE Caleb TRAYLOR Laura ARPAN cali CURLEY Chien-Fei CHEN julia DAY Richard FEIOCK Mirsad HADZIKADIC William j. TOLONE Stan INGMAN Dale YEATTS Omer T. KARAGUZEL Khee Poh LAM Carol MENASSA Svetlana PEVNITSKAYA Thomas SPIEGELHALTER Wei YAN Yimin ZHU Yong X. TAO Department of Mechanical and Energy Engineering University of North Texas Denton Texas 76203 USA College of Communication & Information Florida State University Tallahassee FL 32306 USA School of Public and Environmental Affairs Indiana University-Purdue University Indianapolis Indianapolis 1N 46202 USA Department of Sociology University of Tennessee Knoxville Knoxville TN 37996 USA Human Ecology Department of Construction Management Washington State University Pullman WA 99164 USA Reubin O'D. Askew School of Public Administration and Policy Florida State University Tallahassee FL 32306 USA College of Computing and Informatics University of North Carolina Charlotte Charlotte NC 28223 USA Department of Gerontology University of North Texas Denton TX 76203 USA Department of Gerontology and Department of Sociology University of North Texas Denton TX 76203 USA CMU School of Architecture Carnegie Mellon University Pittsburgh PA 15213 USA School of Design & Environment National University of Singapore Singapore 117566 Singapore Department of Civil and Environmental Engineering University of Michigan Ann Arbor MI 48109 USA Department of Economics Florida State University Tallahassee FL 32306 USA Department of Architecture Florida Intemational University Miami FL 33199 USA College of Architecture Texas A & M University College Station TX 77843 USA Bert S. Turner Department of Construction Management Louisiana State University Baton Rogue LA 70803 USA College of Engineering and Computing Nova Southeastern University Davie FL 33314 USA

This paper contributes an inclusive review of scientific studies in the field of sustainable human building ecosystems （SHBEs）. Reducing energy consumption by making buildings more energy efficient has been touted as an easily attainable approach to promoting carbon-neutral energy societies. Yet, despite significant progress in research and technology development, for new buildings, as energy codes are getting more stringent, more and more technologies, e.g., LED lighting, VRF systems, smart plugs, occupancy-based controls, are used. Nevertheless, the adoption of energy efficient measures in buildings is still limited in the larger context of the developing countries and middle income/low-income population. The objective of Sustainable Human Building Ecosystem Research Coordination Network （SHBE-RCN） is to expand synergistic investigative podium in order to subdue barriers in engineering, architectural design, social and economic perspectives that hinder wider application, adoption and subsequent performance of sustainable building solutions by recognizing the essential role of human behaviors within building-scale ecosystems. Expected long-term outcomes of SHBE-RCN are collaborative ideas for transformative technologies, designs and methods of adoption for future design, construction and operation of sustainable buildings.

关键词： sustainability building energy modeling（BEM） occupant behaviors （OB） sustainable ecosystems System for the Observation of Populous Heterogeneous Information （SOPHI）

Has global deforestation accelerated due to the COVID-19 pandemic?

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journal of Forestry Research 2023年第4期34卷 1153-1165页

作者： jonnathan Céspedes janelle M.Sylvester Lisset Pérez-Marulanda Paula Paz-Garcia Louis Reymondin Mehran Khodadadi jhon j.Tello Augusto Castro-Nunez The Alliance of Bioversity International and International Center for Tropical Agriculture(CIAT) Km 17 Recta Cali-PalmiraCaliColombia Department of Geosciences and Natural Resource Management University of CopenhagenCopenhagenDenmark Leibniz Centre for Agricultural Landscape Research(ZALF) MünchebergGermany

As the COVID-19 pandemic unfolded,questions arose as to whether the pandemic would amplify or pacify tropical deforestation.Early reports warned of increased deforestation rates;however,these studies were limited to a few months in 2020 or to selected regions.To better understand how the pandemic infl uenced tropical deforestation globally,this study used historical deforestation data(2004–2019)from the Terra-i pantropical land cover change monitoring system to project expected deforestation trends for 2020,which were used to determine whether observed deforestation deviated from expected trajectories after the fi rst COVID-19 cases were reported.Time series analyses were conducted at the regional level for the Americas,Africa and Asia and at the country level for Brazil,Colombia,Peru,the Democratic Republic of Congo and Indonesia.Our results suggest that the pandemic did not alter the course of deforestation trends in some countries(e.g.,Brazil,Indonesia),while it did in others(e.g.,Peru).We posit the importance of monitoring the long-term eff ects of the pandemic on deforestation trends as countries prioritize economic recovery in the aftermath of the pandemic.

关键词： Deforestation COVID-19 Time series Terra-i Drivers of deforestation monitoring

Systematic study of the dependence of magnetic and structural properties of Nd2Fe14B powders on the average particle size

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journal of Rare Earths 2020年第9期38卷 961-968,I0002页

作者： j.F.Durán Perdomo G.A.Pérez Alcázar H.D.Colorado j.A.Tabares L.E.Zamora j.j.S.Garitaonandia Department of Physics University of ValleA.A.25360 CaliColombia Applied Physics II Department Basque Country University48080 BilbaoSpain

A systematic study of the magnetic and structural properties dependence on the particle size was realized.For this,commercial NdFeB powder was separated into five different mean particle sizes using sieves.Besides,from the original powder,eleven samples were also produced by mechanical milling assisted by surfactant,using various milling times.A total of sixteen samples were studied by scanning electron microscopy(SEM),X-ray diffraction(XRD),vibrating sample magnetometry(VSM),and Mdssbauer spectrometry(MS).The particle sizes of the samples vary from the micrometer to the nanometer scale.The crystallite size decreases with decreasing particle size.XRD result indicates that the Nd2Fe14B phase is found in all the samples,and the presence of this phase is also corroborated by MS using six sextets for fitting their spectra,with an additional singlet corresponding to the Nd1.1Fe4B4 phase.The mean hyperfine magnetic field increases with increasing particle size because the magnetic dipolar interaction between the magnetic moment of the particles increases with particle size.From the VSM measurements the magnetic energy density(BH)max values were calculated for different particle sizes,and their maximum value of 34.45 MGOe is obtained for the sample with the particle size of 60μm.

关键词： Hard magnetic material XRD Mossbauer spectrometry Magnetization Particle size Rare earths

Fragile X Messenger Ribonucleoprotein 1(FMR1), a novel inhibitor of osteoblast/osteocyte differentiation, regulates bone formation, mass, and strength in young and aged male and female mice

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Bone Research 2023年第2期11卷 384-397页

作者： Padmini Deosthale julián Balanta-Melo Amy Creecy Chongshan Liu Alejandro Marcial Laura Morales julita Cridlin Sylvia Robertson Chiebuka Okpara David j.Sanchez Mahdi Ayoubi joaquín N.Lugo Christopher j.Hernandez joseph M.Wallace Lilian I.Plotkin Department of Anatomy Cell Biology&PhysiologyIndiana University School of MedicineIndianapolisIN46202USA Roudebush Veterans Administration Medical Center IndianapolisIN46202USA Indiana Center for Musculoskeletal Health IndianapolisIN46202USA Universidad del Valle School of Dentistry Cali 760043Colombia Department of Biomedical Engineering Indiana University-Purdue University Indianapolis46202 IndianapolisIN46202USA Sibley School of Mechanical and Aerospace Engineering Cornell UniversityIthacaNY 14853USA Department of Materials Science and Engineering Cornell UniversityIthacaNY14853USA Department of Psychology and Neuroscience Baylor UniversityWacoTX76798USA

Fragile X Messenger Ribonucleoprotein 1(FMR1)gene mutations lead to fragile X syndrome,cognitive disorders,and,in some individuals,scoliosis and craniofacial abnormalities.Four-month-old(mo)male mice with deletion of the FMR1 gene exhibit a mild increase in cortical and cancellous femoral bone mass.However,consequences of absence of FMR1 in bone of young/aged male/female mice and the cellular basis of the skeletal phenotype remain unknown.We found that absence of FMR1 results in improved bone properties with higher bone mineral density in both sexes and in 2-and 9-mo mice.The cancellous bone mass is higher only in females,whereas,cortical bone mass is higher in 2-and 9-mo males,but higher in 2-and lower in 9-mo female FMR1-knockout mice.Furthermore,male bones show higher biomechanical properties at 2mo,and females at both ages.Absence of FMR1 increases osteoblast/mineralization/bone formation and osteocyte dendricity/gene expression in vivo/ex vivo/in vitro,without affecting osteoclasts in vivo/ex vivo.Thus,FMR1 is a novel osteoblast/osteocyte differentiation inhibitor,and its absence leads to age-,site-and sex-dependent higher bone mass/strength.

关键词： female Messenger agile

Butterfly Optimized Feature Selection with Fuzzy C-Means Classifier for Thyroid Prediction

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Intelligent Automation & Soft Computing 2023年第3期35卷 2909-2924页

作者： S.j.K.jagadeesh Kumar P.Parthasarathi Mehedi Masud jehad F.Al-Amri Mohamed Abouhawwash Department of Computer Science and Engineering Kathir College of EngineeringCoimbatore641062India Department of Computer science and Engineering Bannari Amman Institute of TechnologySathyamangalam638401TamilnaduIndia Department of Computer Science College of Computers and Information TechnologyTaif UniversityTaif21944Saudi Arabia Department of Information Technology College of Computers and Information TechnologyTaif UniversityP.O.BOX 11099Taif21944Saudi Arabia Department of Mathematics Faculty of ScienceMansoura UniversityMansoura 35516Egypt Department of Computational Mathematics Scienceand Engineering(CMSE)Michigan State UniversityEast LansingMI48824USA

The main task of thyroid hormones is controlling the metabolism rate of humans,the development of neurons,and the signiﬁcant growth of reproductive activities.In medical science,thyroid disorder will lead to creating thyroiditis and thyroid cancer.The two main thyroid disorders are hyperthyroidism and hypothyroidism.Many research works focus on the prediction of thyroid disorder.To improve the accuracy in the classiﬁcation of thyroid disorder this paper pro-poses optimization-based feature selection by using differential evolution with the Butterﬂy optimization algorithm(DE-BOA).For the classiﬁer fuzzy C-means algorithm(FCM)is used.The proposed DEBOA-FCM is evaluated with para-metric metric measures of sensitivity,speciﬁcity,and accuracy.In this work,the thyroid disease dataset collected from the machine learning University of cali-fornia Irvine(UCI)database was used.The accuracy rate for the Differential Evo-lutionary algorithm got 0.884,the Butterﬂy optimization algorithm got 0.906,Fuzzy C-Means algorithm got 0.899 and DEBOA+Focused Concept Miner(FCM)proposed work 0.943.

关键词： Fuzzy butterﬂy differential evolution thyroid hyperthyroid

53例肾脏混合性上皮和间质肿瘤的临床病理研究

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临床与实验病理学杂志 2017年第1期33卷 83-83页

作者： caliò A Eble j N Grignon D j 魏建国刘勇浙江省绍兴市人民医院病理科绍兴312000 江西省人民医院病理科南昌330006

肾脏混合性上皮和间质肿瘤是一种罕见的复杂肿瘤，作者分析53例该肿瘤（女性46例、中位年龄49岁）的大体观察、组织形态学及免疫组化特征。大体观察最常见的表现是实性和囊性（47％）。最常见的间质类型为少细胞的纤维性间质和富于细胞... 详细信息

肾脏混合性上皮和间质肿瘤是一种罕见的复杂肿瘤，作者分析53例该肿瘤（女性46例、中位年龄49岁）的大体观察、组织形态学及免疫组化特征。大体观察最常见的表现是实性和囊性（47％）。最常见的间质类型为少细胞的纤维性间质和富于细胞的梭形细胞间质，其次是伴平滑肌分化的间质、水肿样间质和出现脂肪组织。

关键词：间质肿瘤混合性临床病理上皮肾脏梭形细胞组织形态学平滑肌分化

Seroprevalence of viral and bacterial pathogens among malaria patients in an endemic area of southern Venezuela

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Infectious Diseases of Poverty 2023年第2期12卷 130-131页

作者： David A.Forero-Peña Fhabián S.Carrión-Nessi Mary Lopez-Perez Marisol Sandoval-de Mora Iván D.Amaya Ángel F.Gamardo Melynar Chavero Luisamy Figuera María V.Marcano Natasha A.Camejo-Ávila Mariana Hidalgo Cariagne j.Arenas Myriam Arévalo-Herrera Sócrates Herrera Biomedical Research and Therapeutic Vaccines Institute Ciudad Bolivar Venezuela Internal Medicine Department “Ruiz y Páez” University Hospital Complex Ciudad Bolivar Venezuela “Dr. Francisco Battistini Casalta” Health Sciences School University of Oriente – Bolivar Nucleus Ciudad Bolivar Venezuela Department of Immunology and Microbiology Faculty of Health and Medical Sciences Centre for Medical Parasitology University of Copenhagen Copenhagen Denmark Immunoparasitology Laboratory Microbiology and Cell Biology Centre Venezuelan Institute for Scientific Research Miranda Venezuela Caucaseco Scientific Research Centre Cali Colombia

Background Malaria remains a leading public health problem worldwide.Co-infections with other pathogens complicate its diagnosis and may modify the disease’s clinical course and management.Similarities in malaria clinical presentation with other infections and overlapping endemicity result in underdiagnosis of co-infections and increased mortality.Thus,the aim of this study was to determine the seroprevalence of viral and bacterial pathogens among diagnosed malaria patients in malaria-endemic areas in Venezuela.Methods A cross-sectional study was conducted on malaria patients attending three reference medical centres in Ciudad Bolivar,Venezuela.Clinical evaluation and laboratory tests for dengue virus(DENV),chikungunya virus(CHIKV),viral hepatitis[hepatitis A virus(HAV),hepatitis B virus(HBV),and hepatitis C virus(HCV)],and leptospirosis(LEP)were performed by enzyme-linked immunosorbent assays.Previous exposure to these pathogens was defined by the presence of specific immunoglobulin(Ig)G,and co-infection or recent exposure(CoRE)was determined by the presence of specific IgM alone or IgM+IgG.Data analysis considered descriptive statistics.Parameter distribution was statistically evaluated using Kolmogorov–Smirnov test and the necessary comparison tests.Odds ratio(OR)for complications was determined according to CoRE presence with a 95%confidence interval(CI).Results A total of 161 malaria patients were studied,66%infected with Plasmodium vivax,27%with P.falciparum,and 7.5%harboured P.vivax/P.falciparum mixed infection.Previous exposure to DENV(60%)and CHIKV(25%)was frequent.CoRE was confirmed in 55 of the 161 malaria patients(34%)and were more frequent in P.falciparum(49%)than in P.vivax(29%)and mixed malaria patients(25%)(OR=2.43,95%CI:1.39–4.25,P=0.018).The most frequent CoRE was DENV(15%),followed by HAV(12%),HBV(6.2%),CHIKV(5.5%),and LEP(3.7%);HCV CoRE was absent.Complicated malaria was significantly more frequent in patients with CoRE(56%)than those without CoRE(36%;OR=2.31,95%CI:1.18–4.92,P=0.013).Conclusio

关键词： Malaria Seroprevalence Co-infection Chikungunya Dengue Hepatitis Venezuela

Effect of cobalt substitution on structure and magnetic properties of Nd0.4Zr0.6Fe10-xCoxSi2（x =0-3） alloys and their ribbons

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journal of Rare Earths 2019年第10期37卷 1096-1101页

作者： M.Gjoka C.Sarafidis G.Giannopoulos D.Niarchos G.Hadjipanayis j.A.Tabares G.A.Pérez Alcázar L.E.Zamora Institute of Nanoscience and Nanotechnology NCSR Demokritos15310AthensGreece Department of Physics and Astronomy University of DelawareNewarkDEUSA Department of Physics Aristotle University of Thessaloniki54124ThessalonikiGreece Departamento de Física Universidad del ValleA.A.25360CaliColombia

This study reports the stabilization of the RFe12-type based compounds where part of R and Fe are substituted with Zr and Co and Si, respectively, in order to examine whether these rare-earth-lean materials are suitable for applications as permanent magnets. Structural and magnetic characterization of the family of alloys with the general formula Nd0.4 Zr0.6 Fe10-xCoxSi2(x = 0 -3) and their melt-spun ribbons were carried out using X-ray diffraction and M€ossbauer spectroscopy. The ThMn12-type structure is obtained for all samples as the majority phase with a minority a-Fe(CoSi) phase(less than 5 wt%) as it was estimated by XRD for x = 1 and 2. The Curie temperature increases linearly with Co substitution from 561 K for x = 0 to 712 K for x = 3. The saturation magnetization decreases slightly from 130.5(x =1)to 129.1 A·m2/kg(x=3), while the anisotropy field is following the same trend.

关键词： Permanent magnets X-ray diffraction M€ossbauer spectroscopy Rare-earth-lean magnetic materials Magnetic properties Melt-spun ribbons