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Taxonomic dependency and spatial heterogeneity in assembly mechanisms of bacteria across complex coastal waters

Ecological Processes 2024年第1期13卷 80-97页

作者： Huizhen Yan Dandan Lin Gaoke Gu Yujie huang Xuya Hu Zhenhao Yu Dandi Hou Demin Zhang Barbara j.Campbell Kai Wang State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products School of Marine SciencesNingbo University Key Laboratory of Marine Biotechnology of Zhejiang Province School of Marine Sciences Ningbo University Department of Biological Sciences Clemson University

Background Understanding community assembly mechanisms across taxa and space is fundamental for microbial ecology. However, the variability and determinants of assembly processes over taxa and space remain unclear. Here, we investigated taxonomic dependency and spatial heterogeneity in bacterial assembly mechanisms across coastal waters in the East China Sea using neutral and null models with customized visualization *** Overall, bacterial assembly mechanisms varied across broad taxonomic groups(phyla and proteobacterial classes) and space at the regional scale. A determinism–stochasticity balanced mechanism governed total bacterial assembly, while taxonomic dependency existed in assembly mechanisms and ecological processes. Among community ecological features, niche breadth and negative-to-positive cohesion ratio were strongly associated with the determinism-to-stochasticity ratio of bacterial groups. Bacterial assembly mechanisms commonly exhibited spatial heterogeneity, the extent and determinants of which varied across taxonomic groups. Spatial assembly of total bacteria was directly driven by many environmental factors and potential interactions between taxa, but not directly by geographic factors. Overall, the bacterial groups with higher spatial heterogeneity in assembly mechanisms were more related to environmental and/or geographic factors(except Bacteroidetes), while those with lower heterogeneity were more related to ecological *** Our results confirm the pervasiveness of taxonomic dependency and spatial heterogeneity in bacterial assembly, providing a finer understanding about regulation across complex coastal waters.

关键词： Bacterioplankton Assembly mechanism Niche breadth Null model Environmental gradient Biogeography

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Optimizing theπ-Bridge of Non-fullerene Acceptors to Suppress Dark Current in NIR Organic Photodetectors

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Chemical Research in Chinese Universities 2024年第4期40卷 712-721页

作者： SHAO Lin huang Yijun HONG Ling XU Zishuo YANG Xiye LIU Chunchen huang Fei CAO Yong State Key Laboratory of Luminescent Materials and Devices Institute of Polymer Optoelectronic Materials and DevicesSchool of Materials Science and EngineeringSouth China University of TechnologyGuangzhou510640P.R.China Lumidar Technology Co. Ltd.Guangzhou510530P.R.China

Recently,the rapid development of non-fullerene acceptors(NFAs)has laid the foundation for performance improvements in near-infrared(NIR)organic photodetectors(OPDs).However,reducing the bandgap of NFAs to achieve strong absorption in the shorter-wave region usually leads to increased dark current density(j_(d))and decreased responsivity(R),severely limiting the detectivity(D*)of *** date,it remains challenging to manipulate the j_(d) of NIR-OPDs through rational structure engineering of ***,three NIR-NFAs,namely bis(2-decyltetradecyl)4,4′-(2′,7′-di-tert-butylspiro[cyclopenta[2,1-b:3,4-b′]dithiophene-4,9′-fluorene]-2,6-diyl)bis(6-(((Z)-1-(dicyanomethylene)-5,6-difluoro-3-oxo-1,3-dihydro-2H-inden-2-ylidene)methyl)thieno[3,4-b]thiophene-2-carboxylate)(TSIC-4F),bis(2-decyltetradecyl)6,6′-(2′,7′-di-tert-butylspiro[cyclopenta[2,1-b:3,4-b′]dithiophene-4,9′-fluorene]-2,6-diyl)bis(4-(((Z)-1-(dicyanomethylene)-5,6-difluoro-3-oxo-1,3-dihydro-2H-inden-2-ylidene)methyl)thieno[3,4-b]thiophene-2-carboxylate)(STIC-4F),and 2,2′-((2Z,2′Z)-(((2′,7′-di-tert-butylspiro[cyclopenta[2,1-b:3,4-b′]dithiophene-4,9′-fluorene]-2,6-diyl)bis(2,3-bis(5-(2-butyloctyl)thiophen-2-yl)thieno[3,4-b]pyrazine-7,5-diyl))bis(metha-neylylidene))bis(5,6-difluoro-3-oxo-2,3-dihydro-1H-indene-2,1-diylidene))dimalononitrile(TPIC-4F),were designed using the thieno[3,4-b]thiophene(TT)and thieno[3,4-b]pyrazine(TPy)derivatives as theπ-*** to the intramolecular S-S and S-N interactions,STIC-4F and TPIC-4F exhibited smaller backbone distortions than TSIC-4F.A significantly red-shifted absorption with a peak at 1015 nm was observed in TPIC-4F film,larger than that(ca.960 nm)for TSIC-4F and STIC-4F ***,OPDs operating in a photovoltaic mode were successfully fabricated,and TPIC-4F-based OPDs achieved the lowest j_(d) of 3.18×10^(-8) A/cm^(2) at-0.1 ***,although TPIC-4F-based OPDs exhibited the lowest R,higher shot-noise-limited specific detectivity(D_(sh)*)in 1000-1200 nm co

关键词： π-Bridge Near-infrared Non-fullerene acceptor Dark current Organic photodetector

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A ratiometric fluorescent probe for hypoxanthine detection in aquatic products based on the enzyme mimics and fluorescence of cobalt-doped carbon nitride

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Food Science and Human Wellness 2024年第2期13卷 879-884页

作者： Xin Wang Chengyi Hong Zhengzhong Lin Zhiyong huang College of Ocean Food and Biological Engineering Jimei UniversityXiamen 361021China

A ratiometric fluorescent probe for hypoxanthine(Hx)detection was established based on the mimic enzyme and fluorescence characteristics of cobalt-doped graphite-phase carbon nitride(Co doped g-C_(3)N_(4)).In addition to emitting strong fluorescence,the peroxidase activity of Co doped g-C_(3)N_(4)can catalyze the reaction of O-phenylenediamine and H_(2)O_(2)to produce diallyl phthalate which can emit yellow fluorescence at 570 *** the decomposition of Hx by xanthine oxidase,Hx can be indirectly detected by the generating hydrogen peroxide based on the measurement of fluorescent ratio I(F_(570)/F_(370)).The linear range was 1.7-272.2 mg/kg(R^(2)=0.997),and the detection limit was 1.52 mg/kg(3σ/K,n=9).The established method was applied to Hx detection in bass,grass carp,and shrimp,and the data were verified by *** result shows that the established probe is sensitive,accurate,and reliable,and can be used for Hx detection in aquatic products.

关键词： Co doped g-C_(3)N_(4) Ratiometric fluorescent probe Hypoxanthine Freshness Aquatic products

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Construction of 2D/2D Ti_(3)C_(2)T_(x)MXene/CdS heterojunction with photothermal effect for efficient photocatalytic hydrogen production

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journal of Materials Science & Technology 2024年第4期171卷 1-9页

作者： T.Y.huang Z.Yang S.Y.Yang Z.H.Dai Y.j.Liu j.H.Liao G.Y.Zhong Z.j.Xie Y.P.Fang S.S.Zhang Key Laboratory for Biobased Materials and Energy of Ministry of Education Guangdong Laboratory for Lingnan Modern AgricultureCollege of Materials and EnergySouth China Agricultural UniversityGuangzhou 510643China School of Physics and Optoelectronics South China University of TechnologyGuangzhou 510640China School of Chemical Engineering and Energy Technology Guangdong Provincial Key Laboratory of Distributed Energy SystemsDongguan University of TechnologyDongguan 523808China

The insensitivity of semiconductors to visible and infrared light is a key constraint on the utilization of light energy in photocatalytic *** photocatalysts with full-spectrum absorption through surface engineering is an effective approach to fully harnessing light energy in semiconductor ***,a novel stable Ti_(3)C_(2)T_(x)MXene/CdS heterojunction catalyst is obtained by in-situ epitaxial growth of two-dimensional(2D)CdS nanosheets on 2D MXene interface via a solvothermal *** exceptional light absorption properties of MXene confer outstanding full-spectrum driven photocat-alytic hydrogen evolution capability upon the heterogeneous *** unique 2D/2D structure effectively mitigated the recombination of photogenerated carriers,enhancing the photocatalytic performance of the ***,the composite catalyst exhibits a significantly higher surface temperature of 80.4℃under visible light irradiation at an intensity of 0.1 W/cm^(2),which is 1.84 times higher than that of *** irradiation of visible and near infrared light,the composite catalyst with photothermal ef-fect demonstrates a remarkable hydrogen evolution rate of 65.4 mmol g^(-1)h^(-1),which is 7.2 times higher than that of CdS *** study introduces a novel approach for constructing full-spectrum absorption catalysts and expands the application of the photothermal effect in photocatalytic hydrogen evolution research.

关键词： Two-dimensional structure Heterostructure Photothermal effect Photocatalyst Hydrogen evolution

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Experimental study of face passive failure features of a shallow shield tunnel in coastal backfill sand

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Frontiers of Structural and Civil Engineering 2024年第2期18卷 252-271页

作者： Weifeng QIAN Ming huang Bingnan WANG Chaoshui XU Yanfeng HU School of Civil Engineering Fuzhou UniversityFuzhou 350108China School of Civil Environment and Mining EngineeringUniversity of AdelaideAdelaide 5005Australia China First Highway Xiamen Engineering Co. Ltd.Xiamen 361000China

Face passive failure can severely damage existing structures and underground utilities during shallow shield tunneling,especially in coastal backfill *** this work,a series of laboratory model tests were developed and conducted to investigate such failure,for tunnels located at burial depth ratios for which C/D=0.5,0.8,1,and *** pressures,the evolution of failure processes,the failure modes,and the distribution of velocity fields were examined through model tests and numerical *** support pressure in the tests first rose rapidly to the elastic limit and then gradually increased to the maximum value in all *** maximum support pressure decreased slightly in cases where C/D=0.8,1,and 1.3,but the rebound was insignificant where C/D=*** addition,the configuration of the failure mode with C/D=0.5 showed a wedge-shaped arch,which was determined by the outcropping shear *** configuration of failure modes was composed of an arch and the inverted trapezoid when C/D=0.8,1,and 1.3,in which the mode was divided into lower and upper failure zones.

关键词： tunnel face stability passive failure model test support pressure failure mode

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Copper-Free Resin-Based Braking Materials:A New Approach for Substituting Copper with Fly-Ash Cenospheres in Composites

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Chinese journal of Mechanical Engineering 2024年第2期37卷 401-412页

作者： Kaikui Zheng Youxi Lin Shanmin You Zhiying Ren jianmeng huang School of Mechanical Engineering and Automation Fuzhou UniversityFuzhou 350108China National United Engineering Laboratory for Advanced Bearing Tribology Henan University of Science and TechnologyLuoyang 471023China

Copper particles emitted from braking have become a significant source of environmental ***,copper plays a crucial role in resin-based braking *** high-performance braking materials without copper has become a significant *** this paper,the resin-based braking materials were filled with flyash cenospheres to develop copper-free braking *** effects of fly-ash cenospheres on the physical properties,mechanical and friction and wear properties of braking materials were ***,the wear mechanism of copper-free resin-based braking materials filled with fly-ash cenospheres was *** results indicate that the inclusion of fly-ash cenospheres in the braking materials improved their thermal stability,hardness and impact strength,reduced their density,effectively increased the friction coefficient at medium and high temperatures,and enhanced the heat-fade resistance of the braking *** inclusion of fly-ash cenospheres contributed to the formation of surface friction film during the friction process of the braking materials,and facilitated the transition of form from abrasive wear to adhesive *** 100-350℃,the friction coefficient of the optimal formulation is in the range of 0.57-0.61,and the wear rate is in the range(0.29-0.65)×10^(-7) cm^(3)·N^(-1)·m^(-1),demonstrating excellent resistance to heat-fade and stability in friction *** research proposes the use of fly-ash cenospheres as a substitute for environmentally harmful and expensive copper in brake materials,which not only improves the performance of braking materials but also reduces their costs.

关键词： Fly-ash cenospheres Braking materials Friction and wear Heat-fade resistance Wear form

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Co-incorporation of Chinese milk vetch(Astragalus sinicus L.)and chemical fertilizers alters microbial functional genes supporting short-time scale positive nitrogen priming effects in paddy soils

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Pedosphere 2024年第3期34卷 567-576页

作者： Limin WANG Chunmei HE Dongfeng huang juhua YU Cailing LIU Qinghua LI Yibin huang juan LI Fei WANG Institute of Resources Environment and Soil FertilizerSoil and Fertilizer InstituteFujian Academy of Agricultural SciencesFuzhou 350012(China) School of Resources and Environment Engineering Anhui UniversityHefei 230601(China)

Nitrogen(N)priming is a microbially mediated biochemical process as affected by different incorporation ***,little information is known about the microbial mechanisms driving the response of N priming to co-operation of Chinese milk vetch(CMV,Astragalus sinicus L.)and different rates of chemical fertilizers in paddy soils in South ***,an anaerobic incubation experiment was conducted to study N priming effects(PE)and their relationships with soil microbial functional genes after CMV incorporation alone(M),co-incorporation of CMV with 100%(normal dosage)chemical fertilizers(MC100),and co-incorporation of CMV with 80% chemical fertilizers(MC80).Co-incorporation of CMV and chemical fertilizers enhanced the short-time scale(the first 20 d of incubation)positive PE of N,while no significant differences existed among the three treatments on day 60 or 90 of incubation(P>0.05).Compared with the M treatment,gross priming effect(GPE)in the MC100 and MC80 treatments significantly increased by 34.0% and 31.3%,respectively,and net priming effect(NPE)increased by 47.7% and 47.8%,respectively,during the first 20 d of incubation(P<0.05).This was likely attributed to soil nutrient availability and added substrate *** MC100 and MC80 treatments increased the gdhA gene abundance by 5.0% and 9.8%,increased the gdh2 gene abundance by 12.7% and 45.7%,and increased the nasB gene abundance by 9.5% and 41.4%,respectively,in comparison with the M treatment on day 20 of *** analyses indicated that soil microbial functional genes involved in N mineralization(gdhA and gdh2),assimilatory nitrate reduction(nasB),and nitrification(amoB)were significantly correlated with N priming under different incorporation practices during the incubation period(P<0.05).Thus,co-incorporation of CMV and chemical fertilizers can regulate soil microbial community functional gene structure,which may accelerate mineralization and assimilatory nitrate reduction and inhibit

关键词： green manure gross priming effect incorporation practice microbial mechanism net priming effect rice field soil

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ON MONOTONE TRAVELING WAVES FOR NICHOLSON'S BLOWFLIES EQUATION WITH DEGENERATE p-LAPLACIAN DIFFUSION

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Acta Mathematica Scientia 2024年第4期44卷 1550-1571页

作者： Rui huang Yong WANG Zhuo YIN School of Mathematical Sciences South China Normal UniversityGuangzhou 510631China

We study the existence and stability of monotone traveling wave solutions of Nicholson's blowflies equation with degenerate p-Laplacian *** prove the existence and nonexistence of non-decreasing smooth traveling wave solutions by phase plane analysis ***,we show the existence and regularity of an original solution via a compactness ***,we prove the stability and exponential convergence rate of traveling waves by an approximated weighted energy method.

关键词： degenerate diffusion p-Laplacian traveling waves stability

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Antibacterial and cytotoxic metabolites produced by Streptomyces tanashiensis BYF-112 isolated from Odontotermes formosanus

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Chinese journal of Natural Medicines 2024年第9期22卷 822-830页

作者： WU j. SONG T. ZHANG L. huang Z. huang F. YIN C. ZHANG S. LIU X. ZHANG Y. School of Life Science Anhui Agricultural University Hefei 230036 China School of Pharmacy Anhui Medical University Hefei 230036 China

Chemical investigations of the termite-associated Streptomyces tanashiensis BYF-112 resulted in the discovery of four novel alkaloid derivatives: vegfrecines A and B (1 and 2), exfoliazone A (3), and venezueline H (7), in addition to nine known metabolites (4−6, 8−13). The structures of these compounds were elucidated through comprehensive spectroscopic analysis and comparison with existing literature data. Antibacterial assays revealed that viridomycin A (11) exhibited potent antibacterial activity against Staphylococcus aureus, with a zone of inhibition (ZOI) of 12.67 mm, in comparison to a ZOI of 17.67 mm for the positive control gentamicin sulfate. Viridomycin A (11) showed moderate activity against Micrococcus tetragenus and Pseudomonas syringae pv. actinidae, with ZOI values of 15.50 and 14.33 mm, respectively, which were inferior to those of gentamicin sulfate (34.67 and 24.00 mm). Viridomycin F (12) also exhibited moderate antibacterial effects against S. aureus, M. tetragenus, and P. syringae pv. actinidae, with ZOI values of 8.33, 16.50, and 10.83 mm, respectively. Cytotoxicity assays demonstrated that viridobruunine A (5), exfoliazone (6), viridomycin A (11), and X-14881E (13) exhibited significant cytotoxicity against human malignant melanoma (A375), ovarian cancer (SKOV-3), and gastric cancer (MGC-803) cell lines, with IC50 values ranging from 4.61 to 19.28 μmol·L−1. Furthermore, bioinformatic analysis of the complete genome of S. tanashiensis suggested a putative biosynthetic gene cluster (BGC) responsible for the production of compounds 1−12. These findings indicate that the secondary metabolites of insect-associated S. tanashiensis BYF-112 hold promise as potential sources of novel antibacterial and anticancer agents. © 2024 China Pharmaceutical University

关键词： Alkaloid Antibacterial activity Biosynthetic pathway Cytotoxic activity Odontotermes formosanus Streptomyces tanashiensis

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Multichannel microneedle dry electrode patches for minimally invasive transdermal recording of electrophysiological signals

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Microsystems & Nanoengineering 2024年第3期10卷 119-136页

作者： Zhengjie Liu Xingyuan Xu Shuang huang Xinshuo huang Zhibo Liu Chuanjie Yao Mengyi He jiayi Chen Hui-jiuan Chen jing Liu Xi Xie State Key Laboratory of Optoelectronic Materials and Technologies School of Electronics and Information TechnologyGuangdong Province Key Laboratory of Display Material and TechnologySun Yat-Sen UniversityGuangzhouChina School of Biomedical Engineering Sun Yat-Sen UniversityGuangzhouChina The First Affiliated Hospital of Sun Yat-Sen University GuangzhouChina

The collection of multiple-channel electrophysiological signals enables a comprehensive understanding of the spatial distribution and temporal features of electrophysiological *** approach can help to distinguish the traits and patterns of different ailments to enhance diagnostic *** array electrodes,which can penetrate skin without pain,can lessen the impedance between the electrodes and skin;however,current microneedle methods are limited to single channels and cannot achieve multichannel collection in small ***,a multichannel(32 channels)microneedle dry electrode patch device was developed via a dimensionality reduction fabrication and integration approach and supported by a self-developed circuit system to record weak electrophysiological signals,including electroencephalography(EEG),electrocardiogram(ECG),and electromyography(EMG)*** microneedles reduced the electrode–skin contact impedance by penetrating the nonconducting stratum corneum in a painless *** multichannel microneedle array(MMA)enabled painless transdermal recording of multichannel electrophysiological signals from the subcutaneous space,with high temporal and spatial resolution,reaching the level of a single microneedle in terms of signal *** MMA demonstrated the detection of the spatial distribution of ECG,EMG and EEG signals in live rabbit models,and the microneedle electrode(MNE)achieved better signal quality in the transcutaneous detection of EEG signals than did the conventional flat dry electrode *** work offers a promising opportunity to develop advanced tools for neural interface technology and electrophysiological recording.

关键词： needle rabbit electrode

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