Co-incorporation of Chinese milk vetch(Astragalus sinicus L.)and chemical fertilizers alters microbial functional genes supporting short-time scale positive nitrogen priming effects in paddy soils

作     者：Limin WANG Chunmei HE Dongfeng HUANG Juhua YU Cailing LIU Qinghua LI Yibin HUANG Juan LI Fei WANG Limin WANG;Chunmei HE;Dongfeng HUANG;Juhua YU;Cailing LIU;Qinghua LI;Yibin HUANG;Juan LI;Fei WANG

作者机构：Institute of ResourcesEnvironment and Soil FertilizerSoil and Fertilizer InstituteFujian Academy of Agricultural SciencesFuzhou 350012(China) School of Resources and Environment EngineeringAnhui UniversityHefei 230601(China) 

出 版 物：《Pedosphere》 (土壤圈（英文版）)

年 卷 期：2024年第34卷第3期

页      面：567-576页

核心收录：

学科分类：07[理学] 0713[理学-生态学] 

基　　金：supported by the Youth Talent Program of Fujian Academy of Agricultural Sciences,China(No.YC2019006) the External Cooperation Program of Fujian Academy of Agricultural Sciences,China(No.DWHZ-2022-20) the Foundation of Fujian Academy of Agricultural Sciences,China(No.CXTD2021012-2) the Special Fund of Fundamental Scientific Research at Nonprofit Research Institutions in Fujian,China(No.2022R1025001) the Special Fund of Fundamental Scientific Research at Nonprofit Research Institutions in Fujian,China(No.2022R1025001) the Natural Science Foundation of Fujian Province,China(No.2020J011358) the Modern Agro-Industry Technology Research System-Green Manure,China(No.CARS-22-G-01) “5511”Collaborative Innovation Engineering,China(No.XTCXGC2021009) 

主　　题：green manure gross priming effect incorporation practice microbial mechanism net priming effect rice field soil 

摘      要：Nitrogen(N)priming is a microbially mediated biochemical process as affected by different incorporation ***,little information is known about the microbial mechanisms driving the response of N priming to co-operation of Chinese milk vetch(CMV,Astragalus sinicus L.)and different rates of chemical fertilizers in paddy soils in South ***,an anaerobic incubation experiment was conducted to study N priming effects(PE)and their relationships with soil microbial functional genes after CMV incorporation alone(M),co-incorporation of CMV with 100%(normal dosage)chemical fertilizers(MC100),and co-incorporation of CMV with 80% chemical fertilizers(MC80).Co-incorporation of CMV and chemical fertilizers enhanced the short-time scale(the first 20 d of incubation)positive PE of N,while no significant differences existed among the three treatments on day 60 or 90 of incubation(P0.05).Compared with the M treatment,gross priming effect(GPE)in the MC100 and MC80 treatments significantly increased by 34.0% and 31.3%,respectively,and net priming effect(NPE)increased by 47.7% and 47.8%,respectively,during the first 20 d of incubation(P0.05).This was likely attributed to soil nutrient availability and added substrate *** MC100 and MC80 treatments increased the gdhA gene abundance by 5.0% and 9.8%,increased the gdh2 gene abundance by 12.7% and 45.7%,and increased the nasB gene abundance by 9.5% and 41.4%,respectively,in comparison with the M treatment on day 20 of *** analyses indicated that soil microbial functional genes involved in N mineralization(gdhA and gdh2),assimilatory nitrate reduction(nasB),and nitrification(amoB)were significantly correlated with N priming under different incorporation practices during the incubation period(P0.05).Thus,co-incorporation of CMV and chemical fertilizers can regulate soil microbial community functional gene structure,which may accelerate mineralization and assimilatory nitrate reduction and inhibit