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第9届中国中西医结合学会妇产科专业委员会第二次学术会议

作者： Bin Zhang Nan Chu Xue-Min Qiu Wei Tang Hans-jürgen Gober Da-jin Li Ling Wang Obstetrics and Gynecology Hospital of Fudan University Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases The Academy of Integrative Medicine of Fudan University Hepato-Biliary-Pancreatic Surgery Division Department of Surgery Graduate School of Medicine the University of Tokyo Department of Pharmacy Kepler University Clinic Neuromed Campus Laboratory for Reproductive Immunology Hospital & Institute of Obstetrics and Gynecology IBS Fudan University Shanghai Medical College

Women with advanced maternal age experience more incidences of sterility, miscarriage and embryo aneuploidy. Recent studies have revealed that age-related aneuploidy is mainly caused by the reduction of cohesin complexesin oocyte. Kuntai Capsule is a kind of Traditional Chinese medicine that was firstly used to retrieve the menopausal syndrome. Several studies have indicated its effect to improve the ovarian reserve and oocyte quality in patients undergoing in vitro fertilization-embryo transfer. To evaluate whether Kuntai Capsule could affect cohesion levels and improve the ovarian function of aged mice, 9 month-old C57 bl/6 j mice were divided into different groups and treated with saline, 17β-estradiol, and different concentrations of Kuntai Capsule for 4 weeks. HE staining and anti-VASA staining were used to detect the amounts of follicles. The apoptosis of follicles was measured by anti-γH2 AX staining and TUNEL assay. The density of cohesin subunits-REC8, SMC1β and SMC3 in oocytes were evaluated by immunofluorescent staining. Our findings revealed that administration of Kuntai Capsule increases the total number as well as the number of primordial and primary follicles in a dose-dependent manner. Outcome fromanti-γH2 AX staining and TUNEL assay demonstrated that Kuntai Capsule and E2 partly suppress the apoptosis of follicles. Furthermore, it showed an increased trend of the meiotic-specific cohesin subunits REC8 and SMC1βafter administration with E2 and Kuntai Capsule, and no obvious change in the level of cohesin subunit common to mitotic *** present results revealed that supplement of Kuntai Capsule in aged mice could enhance the number of follicles, suppress apoptosis of oocytes and have a trend to elevate the meiotic-specific cohesin subunits REC8 and SMC1βin oocytes, which implies a beneficial effect on the ovarian function in terms of the quantity and quality of follicles.

关键词： follicular development cohesin oocyte Kuntai Capsule

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恩施渔塘坝富硒碳质岩中硒的赋存状态

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地球化学 2004年第6期33卷 634-640页

作者：朱建明李社红左维 I.Sykorova 苏宏灿郑宝山 j.Pesek 中国科学院地球化学研究所环境地球化学国家重点实验室 Institute of Rock Structure and Mechanics ASCR VHoleovi.kách41 18209 Prague 8Czech Republic 湖北省恩施州卫生防疫站 Faculty of Science Charles University of Prague Albertov 6 Praha 212843 Czech Republic

在渔塘坝富硒碳质岩中发现了自然硒及其系列硒矿物的组合。这些硒矿物有自然硒、方硒铜矿、硒铜蓝、水硒铁石、硒银矿、含硒黄铁矿、含硒黄铜矿和难以确定名称的Fe-Se矿物。其中自然硒、方硒铜矿和硒铜蓝的矿物组合主要分布在断层面附... 详细信息

在渔塘坝富硒碳质岩中发现了自然硒及其系列硒矿物的组合。这些硒矿物有自然硒、方硒铜矿、硒铜蓝、水硒铁石、硒银矿、含硒黄铁矿、含硒黄铜矿和难以确定名称的Fe-Se矿物。其中自然硒、方硒铜矿和硒铜蓝的矿物组合主要分布在断层面附近的碳质硅质岩中,自然硒与方硒铜矿的矿物组合主要分布在高碳质泥岩中,水硒铁石及未确定的Fe-Se矿物主要赋存于露头处的富硒碳质岩中。硒矿物中除自然硒较普遍地存在外,其他矿物并不是均匀地分布于富硒碳质岩中,而是局部性地出现。Cu-Se矿物的大量发育表明铜可能在硒的表生迁移转化中起着某种重要的作用。

关键词：富硒碳质岩硒矿物赋存状态渔塘坝

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基于16S rRNA基因测序研究拉克替醇对小鼠肠道菌群的影响

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中华消化杂志 2018年第6期38卷 386-393页

作者：王亚楠张宏董一凡钱家鸣李景南中国医学科学院北京协和医学院北京协和医院消化内科 100730 中国医学科学院北京协和医学院动物中心 100730

目的探讨益生菌、不同浓度拉克替醇及两者合用对小鼠肠道菌群的影响。方法将50只C57bl/6j小鼠分为空白对照组、益生菌组、标准浓度拉克替醇组、高浓度拉克替醇组、益生菌联合拉克替醇组，每组10只。分别予不干预、1×109 CFU/d益生菌、6... 详细信息

目的探讨益生菌、不同浓度拉克替醇及两者合用对小鼠肠道菌群的影响。方法将50只C57bl/6j小鼠分为空白对照组、益生菌组、标准浓度拉克替醇组、高浓度拉克替醇组、益生菌联合拉克替醇组，每组10只。分别予不干预、1×109 CFU/d益生菌、6.6 g·kg－1·d－1拉克替醇、10.0 g·kg－1·d－1拉克替醇、5×108 CFU/d益生菌联合3.3 g·kg－1·d－1拉克替醇灌胃2周。分别收集灌胃前、灌胃1周、灌胃2周后的粪便标本，以及灌胃2周后的肠黏膜组织，送检16S rRNA基因测序。采用α多样性分析、主成分分析和分类学分析菌群变化。结果灌胃前和灌胃1周后，α多样性分析显示5组小鼠粪便菌群差异均无统计学意义（P＝0.552、0.062）；α多样性分析与主成分分析显示，标准浓度拉克替醇和高浓度拉克替醇组肠黏膜菌群差异均无统计学意义（P＝0.270、0.085）。灌胃1周和2周后，标准浓度和高浓度拉克替醇组分别与空白对照组、益生菌组比较，粪便标本Akkermansia菌属均增加（灌胃1周0.114 3比0.003 9、0.013 1，0.071 3比0.003 9、0.013 1，P〈0.01、P＝0.001和P＝0.001、0.005；灌胃2周0.094 0比0.030 5、0.018 9，0.142 4比0.030 5、0.018 9，P＝0.044、0.016和0.001、〈0.01）；益生菌联合拉克替醇组分别与标准浓度和高浓度拉克替醇组比较，粪便标本拟杆菌属均增加（灌胃1周0.115 9比0.037 5、0.041 6，P＝0.013、0.015；灌胃2周0.058 0比0.023 2、0.014 4，P＝0.047、0.009）。益生菌联合拉克替醇组较早（灌胃1周后）出现毛螺旋菌科相应菌属的增加，灌胃2周后，与空白对照组、标准浓度拉克替醇组和高浓度拉克替醇组相比，益生菌组毛螺旋菌科相应菌属增加（P均〈0.05）。标准浓度拉克替醇组与益生菌组比较，肠黏膜Akkermansia菌属增加（0.018 0比0.001 8，P＝0.012），高浓度拉克替醇组分别与空白对照组、益生菌组相比，肠黏膜Akkermansia菌属亦增加（0.037 0比0.010 0、0.001 8，P＝0.002、〈0.01）。益生菌联合拉克替醇组与空白对照组、标准浓度拉克替醇组、高浓度拉克替醇组相比，以及益生菌组与高浓度拉克替醇组相比，肠黏膜脱铁杆菌科Mucispirillum属均增加（0.040 0比0.014 8、0.013 7、0.009 9，0.019 6比0.009 9；P＝0.041、0.040、0.018、0.011）。结论补充益生菌、拉克替醇或两者联用对小鼠肠道菌群均有明显调节作用，外源性益生菌不易在肠道定植。拉克替醇可明显促进粪便和肠道黏膜中Akkermansia菌属增殖。

关键词：有益菌种拉克替醇肠道菌群 Akkermansia 小鼠

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Technical Standards for Lotus Root-Redswamp Crayfish Integrated Farming

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Agricultural Science & Technology 2015年第7期16卷 1477-1479页

作者：周学金张家宏韩光明寇祥明王守红毕建花朱凌宇徐荣王桂良扬州市农业委员会江苏扬州225001 江苏省生态农业工程技术研究中心江苏扬州225009 江苏里下河地区农业科学研究所江苏扬州225007

Lotus root-redswamp crayfish integrated farming is a kind of ecologicalgrow mode on the basis of paddy field, which is an innovated mode for agricultural structure adjustment and special agriculture with high efficiency. In order to regulate producing technology of this mode, the essay puts forward some technical require- ments, stressing on cultivar selection, field works, farming technology and product quality, which aims at providing technical support for agricultural production.

关键词： Lotus root Redswamp crayfish Integrated farming Standard

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Correction to:EGFR signaling augments TLR4 cell surface expression and function in macrophages via regulation of Rab5a activation

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Protein & Cell 2020年第8期11卷 618-619页

作者： jing Tang Bowei Zhou Melanie j.Scott Linsong Chen Dengming Lai Erica K.Fan Yuehua Li Qiang Wu Timothy R.Billiar Mark A.Wilson Ping Wang jie Fan The Department of Anesthesiology Affiliated hospital of Guangdong Medical UniversityZhanjiang 524000China Department of Surgery University of Pittsburgh School of MedicinePittsburghPA 15213USA Research and Development Veterans Affairs Pittsburgh Healthcare SystemPittsburghPA 15240USA Department of Anesthesiology Nanfang HospitalSouthern Medical UniversityGuangzhou 510515China Department of Thoracic Surgery Shanghai Pulmonary HospitalTongji University School of MedicineShanghai 200433China Department of Cardiovascular Surgery The Children's Hospital of Zhejiang University School of MedicineHangzhou 310052China University of Pittsburgh The Graduate School of Public Health PittsburghPA 15213USA Laboratory of Tropical Biomedicine and Biotechnology School of Tropical Medicine and Laboratory MedicineHainan Medical UniversityHaikou 571199China The Feinstein Institute for Medical Research ManhassetNY 11030USA McGowan Institute for Regenerative Medicine University of PittsburghPittsburghPA 15219USA Key Laboratory of Organ Injury/Protection and Translational Medicine of Zhanjiang Zhanjiang 524000China

Figure *** activation promotes TLR4 phosphorylation and cell surface expression of TLR4 in response to LPS.(A and B)BMDM were treated with LPS(1μg/mL)for 6,12,or 24 h in the presence or absence of pretreatment of PD or TAPI-1.(A)Flow cytometry analysis of cell surface TLR4 intensity in BMDM.(B)Flow cytometry analysis of cell surface TLR4 intensity in BMDM.(C and D)WT(C57bl76)mice were treated with LPS(10 mg/kg,i.p.).In some groups,mice were pretreated with erlotinib(100 mg/kg,gavage administration)at 30 min prior to LPS i.p.

关键词： activation surface phosphorylation

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High-level expression of whiG——A key gene for Streptomyces differentiation in Escherichia coli

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Science China(Life Sciences) 1996年第3期39卷 284-290页

作者：谭华荣田宇清杨海花吴畏董可宁 K. F. Chater and M. j. Buttner Institute of Microbiology Chinese Academy of Sciences Beijing 100080 China john Innes Institute Norwich England)

Six nucleotides located in the region of translation start site of whiG were changed. whiG was amplified by PCR technique. Reformed sequences were determined. This gene was directly subcloned into expression vector pET11c containing strong T7 promoter, and the recombinant plasmid was introduced into E. coli bl21(DE3), which could be induced by IPTG to produce T7 RNA polymerase. The SDS-PAGE result showed that whiG highly expressed in E. coli bl21(DE3), and the yield of whiG product was about 20% of insoluble proteins in cell. whiG product (σwhiG) was further identified by Western blot hybridization after making its antibody. whiG gene was subcloned into Streptomyces plasmid pIj6021, and then it was introduced into sporulation deficient mutant C71 from Streptomyces coelicolor. The result showed that C71 could restore sporulation and σwhiG has biological functions.

关键词： Streptomyces differentiation σ^(whiG) high expression.

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Characterization of a mesenchymal stem cell line that differentiates to bone and provides niches supporting mouse and human hematopoietic stem cells

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Stem Cell Discovery 2012年第1期2卷 5-14页

作者： Sonal R. Tuljapurkar john D. jackson Susan K. Brusnahan Barbara j. O’Kane john G. Sharp 不详

Identification of mouse cell lines with properties of primary multipotential mesenchymal stromal cells (msC) is required to facilitate the use of mouse models for evaluation of mechanisms in bone formation, hematopoiesis and cellular therapies for regenerative medicine. Primary murine msC vary between strains, are difficult to grow in vitro and have inconsistent properties. The main aim of the study was to establish OMA-AD cells as an appropriate model system to conduct studies on msC, bone formation and hematopoiesis. OMA-AD cells were isolated by differential trypsinization of C57bl/6j mouse bone marrow (BM) cells. The cells were then repassaged, cloned and characterized. OMA-AD cells were immortal and non-tumorigenic, differentiated readily to all mesenchymal cell types including bone, supported mouse and human hematopoiesis and were immunosuppressive. Our results demonstrated that OMA-AD cells possessed the properties of primary msC. In addition, these cells grew readily and consistently, thereby facilitating future studies of bone formation, hematopoiesis and mesenchymal cells for regenerative medicine.

关键词： Bone Cell Therapy Hematopoiesis msC Regenerative Medicine Stem Cells

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A novel supplement with yeast b-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice

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journal of Integrative Medicine 2021年第5期19卷 439-450页

作者： Victor Abou Nehmi Gilson Masahiro Murata Ruan Carlos Macêdo de Moraes Gabriely Cristina AlvesLima Danielle Araujo De Miranda Katrin Radloff Raquel Galvao Figuerêdo Costa joyce de Cassia Rosade jesus jéssica Alves De Freitas Nayara Izabel Viana Ruan Pimenta Katia Ramos Moreira Leite joséPinhata Otoch Ana Flávia Marcal Pessoa Laboratory of Medical Investigation(LIM-26) Department of SurgeryUniversity of Sao Paulo Medical SchoolSao PauloSP 01246903Brazil Laboratory of Medical Investigation(LIM-29) Clinic Medical DepartmentUniversity of Sao Paulo Medical SchoolSao PauloSP 01246903Brazil Department of Physiology Escola Paulista de Medicina/Universidade Federal de Sao PauloSP 04023062Brazil Max Rubner-Institut Federal Research Institute of Nutrition and FoodKarlsruheBW 76131Germany Cancer and Inflammation Program Center for Cancer ResearchNational Cancer InstituteNational Institutes of HealthBethesdaMD 20892United States Laboratory of Medical Investigation(LIM-55) Urology DepartmentUniversity of Sao Paulo Medical SchoolSao PauloSP 01246903Brazil D'Or Institute for Research and Education RJ 22281-100Brazil Program in Anesthesiology Surgical Sciencesand Perioperative MedicineUniversity of Sao PauloSao PauloSP 01246903Brazil Brazilian Academic Consortium for Integrative Health(CABSIN) Natural Products CommitteeSao PauloSP 05449-070Brazil

Objective: To evaluate the synergic effects of a novel oral supplement formulation, containing prebiotics,yeast b-glucans, minerals and silymarin(Silybum marianum), on lipid and glycidic metabolism, inflammatory and mitochondrial proteins of the liver, in control and high-fat diet-induced obese ***: After an acclimation period, 32 male C57 bl/6 mice were divided into the following groups:nonfat diet(NFD) vehicle, NFD supplemented, high-fat diet(HFD) vehicle and HFD supplemented. The vehicle and experimental formulation were administered orally by gavage once a day during the last four weeks of the diet(28 consecutive days). We then evaluated energy homeostasis, inflammation, and mitochondrial protein expression in these groups of ***: After four weeks of supplementation, study groups experienced reduced glycemia, dyslipidemia,fat, and hepatic fibrosis levels. Additionally, proliferator-activated receptor-α, AMP-activated protein kinase-1α, peroxisome proliferator-activated receptor γ co-activator-1α, and mitochondrial transcription factor A expression levels were augmented;however, levels of inhibitor of nuclear factor-κB kinase subunit a and p65 nuclear factor-κB expression, and oxidative markers were reduced. Notably, the cortisol/C-reactive protein ratio, a well-characterized marker of the hypothalamic–pituitary–adrenal axis immune interface status, was found to be modulated by the ***: We discovered that the novel supplement was able to modify different antioxidant, metabolic and inflammatory pathways, improving the energy homeostasis and inflammatory status, and consequently alleviated hepatic steatosis.

关键词： Supplement Silymarin Prebiotic Obese Mice Steatosis Inflammation Mitochondrial biogenesis

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小麦品种川麦107对条锈病成株抗性的QTL定位

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中国农业科学 2010年第4期43卷 706-712页

作者：朱华忠王忠伟伍玲 Ravi P Singh j Huerta-Espino 何中虎胡嘉陈放夏先春四川省农业科学院作物研究所中国农业科学院作物科学研究所/国家小麦改良中心 Internationl Maize and Wheat Improvement Center(CIMMYT) Mexico City6-64106600Mexico CIMMYT中国办事处四川大学生命科学学院

【目的】发掘川麦107中的条锈病成株抗性基因及与其紧密连锁的分子标记,为小麦持久抗性育种提供基因和分子标记辅助选择工具。【方法】在墨西哥国际玉米小麦改良中心(CIMMYT)Toluca试验站、中国成都和雅安3个环境下,对川麦107/Avocet-Yr... 详细信息

【目的】发掘川麦107中的条锈病成株抗性基因及与其紧密连锁的分子标记,为小麦持久抗性育种提供基因和分子标记辅助选择工具。【方法】在墨西哥国际玉米小麦改良中心(CIMMYT)Toluca试验站、中国成都和雅安3个环境下,对川麦107/Avocet-YrA组合的F5代重组自交系(recombinant inbred line,RIL)群体进行了小麦条锈病成株抗性鉴定,在此基础上利用SSR标记和复合区间作图法对成株抗性基因进行定位。【结果】在1B染色体长臂远端的Xcwem32和Xgwm818位点之间(连锁距离3.9cM)检测到1个主效QTL,暂定名为***-1bl。该主效QTL在3个试验环境下分别解释19.3%、16.9%和27.4%的表型变异。【结论】***-1bl是川麦107中对条锈病有持久抗性的成株抗性QTL。

关键词：小麦条锈病成株抗性 QTL 川麦107

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剔除CCR5基因供者小鼠细胞加重急性移植物抗宿主疾病

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首都医科大学学报 2006年第4期27卷 488-491页

作者：王昭付丽黄达永 William j Murphy 首都医科大学附属北京友谊医院血液内科美国国立卫生研究院骨髓移植研究所

目的评价供者CCR5在经过强化预处理的骨髓移植动物模型受者体内的作用,为异基因造血干细胞移植的临床应用提供科学依据。方法经过致死剂量照射的BALB/C小鼠接受异基因C57bl/6小鼠骨髓移植。根据回输的细胞不同分为4组:1)B6 CCR5 KO组,... 详细信息

目的评价供者CCR5在经过强化预处理的骨髓移植动物模型受者体内的作用,为异基因造血干细胞移植的临床应用提供科学依据。方法经过致死剂量照射的BALB/C小鼠接受异基因C57bl/6小鼠骨髓移植。根据回输的细胞不同分为4组:1)B6 CCR5 KO组,受者接受C57bl/6 CCR5-/-小鼠骨髓和脾脏细胞;2)B6 WT组,受者接受野生型C57bl/6小鼠骨髓和脾脏细胞;3)B6 CCR5 KO BMC组,受者只接受C57bl/6 CCR5-/-小鼠骨髓细胞;4)B6 WT BMC组,受者只接受野生型C57bl/6小鼠骨髓细胞。结果与B6 WT组比较,B6 CCR5 KO组小鼠以更快的速度死于急性移植物抗宿主疾病(GVHD);其受者体内的CD8+T细胞大量增生;T细胞恢复后产生更多的干扰素-γ(INF-γ)和肿瘤坏死因子-α(TNF-α),T细胞有丝分裂原刀豆素水平处于较高水平,进一步促进T细胞增生。组织学检测提示移植剔除CCR5基因受者细胞的小鼠肾脏出现病理损伤,肝脏存在更为严重的病理变化。结论剔除CCR5基因的异基因骨髓移植使GVHD发病率增加,供者CD8+T细胞在受者体内增生加重肝肾损害。提示CCR5在异基因骨髓移植中起着重要作用。

关键词： CCR5 移植物抗宿主疾病异基因造血干细胞移植 CD8^+T细胞

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