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Synthetic and Systems Biotechnology 2020年第4期5卷 252-267页

作者： Stephanie D.cole Aleksandr E.Miklos Abel c.chiao Zachary Z.Sun Matthew W.Lux US Army combat capabilities Development command chemical Biological center 8567 Ricketts Point RoadAberdeen Proving GroundMD21010USA Division of Biology and Biological Engineering California Institute of TechnologyPasadenaCAUSA Synvitrobio Inc. San FranciscoCAUSA 8567 Ricketts Point Road Bldg E3549 Room B140APGMD21010USA

cell-free systems that mimic essential cell functions,such as gene expression,have dramatically expanded in recent years,both in terms of applications and widespread *** we provide a review of cell-extract methods,with a specific focus on prokaryotic ***,we describe the diversity of Escherichia coli genetic strains available and their corresponding *** then trace the history of cell-extract methodology over the past 20 years,showing key improvements that lower the entry level for new ***,we survey the rise of new prokaryotic cell-free systems,with associated methods,and the opportunities ***,we use this historical perspective to comment on the role of methodology improvements and highlight where further improvements may be possible.

关键词： cell-free systems cell-free expression cell-free extract Synthetic biology Methods

A functional tacrolimus-releasing nerve wrap for enhancing nerve regeneration following surgical nerve repair

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Neural Regeneration Research 2025年第1期20卷 291-304页

作者： Simeon c.Daeschler Katelyn j.W.So Konstantin Feinberg Marina Manoraj.j.nny cheung j.nnifer Zhang Kaveh Mirmoeini j.aul Santerre Tessa Gordon Gregory HBorschel SickKids Research Institute Neuroscience and Mental Health ProgramTorontoONCanada Institute of Biomaterials and Biomedical Engineering University of TorontoTorontoONCanada Indiana University School of Medicine IndianapolisINUSA Division of Plastic and Reconstructive Surgery the Hospital for Sick ChildrenTorontoONCanada Translational Biology and Engineering Program Ted Rogers Centre for Heart ResearchInstitute of Biomedical EngineeringFaculty of DentistryUniversity of TorontoTorontoONCanada

Axonal regeneration following surgical nerve repair is slow and often incomplete,resulting in poor functional recovery which sometimes contributes to lifelong ***,there are no FDA-approved therapies available to promote nerve *** accelerates axonal regeneration,but systemic side effects presently outweigh its potential benefits for peripheral nerve *** authors describe herein a biodegradable polyurethane-based drug delivery system for the sustained local release of tacrolimus at the nerve repair site,with suitable properties for scalable production and clinical application,aiming to promote nerve regeneration and functional recovery with minimal systemic drug *** is encapsulated into co-axially electrospun polycarbonate-urethane nanofibers to generate an implantable nerve wrap that releases therapeutic doses of bioactive tacrolimus over 31 *** and drug loading are adj.stable for applications in small and large caliber nerves,and the wrap degrades within 120 days into biocompatible *** released from the nerve wrap promotes axon elongation in vitro and accelerates nerve regeneration and functional recovery in preclinical nerve repair models while off-target systemic drug exposure is reduced by 80%compared with systemic *** its surgical suitability and preclinical efficacy and safety,this system may provide a readily translatable approach to support axonal regeneration and recovery in patients undergoing nerve surgery.

关键词： biodegradable local drug delivery nerve inj.ry nerve regeneration nerve wrap tacrolimus

controversies in fecal incontinence

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World j.urnal of Gastroenterology 2025年第3期31卷 113-119页

作者： Andreia Albuquerque Satish S c Rao School of Medicine and Biomedical Sciences Fernando Pessoa UniversityGondomar 4420-096PortoPortugal Precancerous Lesions and Early cancer Management Research Group RISE@cI-IPO(Health Research Network) Portuguese Oncology Institute of PortoPorto 4200-072Portugal Division of Gastroenterology and Hepatology Department of Internal MedicineAugusta UniversityAugustaGA 30912United States

Fecal incontinence is a common condition that can significantly impact patients’quality of *** anal sphincter inj.ry and anorectal surgeries are common *** ultrasound and anorectal manometry are important diagnostic tools for evaluating *** are various treatment options,including diet,lifestyle modifications,drugs,biofeedback therapy,tibial and sacral nerve neuromodulation therapy,and *** this editorial,we will discuss current controversies and novel approaches to fecal *** for asymptomatic anal sphincter defects after obstetric anal sphincter inj.ry and in patients with inflammatory bowel disease is not generally recommended,but may be helpful in selected *** Garg incontinence score is a new score that includes the assessment of solid,liquid,flatus,mucous,stress and urge fecal *** tests such as translumbosacral anorectal magnetic stimulation and novel therapies such as translumbosacral neuromodulation therapy are promising diagnostic and treatment options,for both fecal incontinence and *** biofeedback therapy can overcome some limitations of the office-based *** muscle-derived cell implantation of the external anal sphincter has been further studied as a possible treatment *** neuromodulation may be useful in scleroderma,congenital fecal incontinence and inflammatory bowel disease but merits further study.

关键词： Fecal incontinence Endoanal ultrasound Anal sphincter defects Biofeedback Neuropathy Sacral neuromodulation

HYERS—ULAM—RASSIAS STABILITY OF QUADRATIc FUNcTIONAL EQUATIONS IN BANAcH MODULES OVER A c^＊—ALGEBRA

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chinese Annals of Mathematics,Series B 2003年第2期24卷 261-266页

作者： c. PARK j. PARK j. SHIN Department of Mathematics Chungnam National University DaeJeon 305-764 South Korea.Department of Mathematics Chungnam National University DaeJeon 305-764 South Korea.Department of Mathematics Chungnam National University DaeJeon 305-764 South Korea.

The authors prove the Hyers-Ulam-Rassias stability of the quadratic mapping in Banachmodules over a unital c*-algebra, and prove the Hyers-Ulam-Rassias stability of the quadraticmapping in Banach modules over a unital... 详细信息

关键词： Stability B-quadratic Functional equation Banach module over Banach algebra

High-performance isotope-labeling liquid chromatography mass spectrometry for investigating the effect of drinking Goj. tea on urine metabolome profiling

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Science china chemistry 2014年第5期57卷 678-685页

作者： TSENG chiao-Li LI Liang Department of chemistry University of Alberta

Urine is a human biofluid that is widely used for metabolomics research on disease biomarker ***,the metabolome profiles generated from comparative groups of individuals should mainly consist of the endogenous human metabolites that reflect the healthy states of the ***,external factors,such as diet,may alter the urine metabolome profile by either introducing a significant amount or variety of exogenous metabolites to urine or inducing changes of the metabolome ***,strict control of the external factors during the sample collection process is critical for urine metabolomics aimed at discovery of disease *** this work,we describe a study to determine the effect of drinking Goj. tea,which is considered a nutritional supplement drink in some regions of the world,on urine metabolome *** purpose of this work is not to determine the nutritional values of Goj. tea,but to investigate whether drinking a moderate amount of Goj. tea 1-3 h(short-term effect)or 12 h(longer-term effect)before urine collection can cause significant variations of urine metabolome profiles.A highly sensitive dansylation isotope labeling liquid chromatography mass spectrometry(Lc-MS)method was used to determine the urine metabolomes before and after drinking Goj. *** the studies of the short term(cts of drinking Goj. tea,it is clear that the consumption of a moderate amount of Goj. tea does not affect the urine metabolome *** for 12 h should be sufficient to remove any potential interference of Goj. metabolites from the human urine metabolome profile.

关键词： metabolomics urine metabolites mass spectrometry liquid chromatography isotope labeling Goj. diet

Molecular level simulations on multi-component systems --a morphology prediction method

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Frontiers of chemical Science and Engineering 2013年第1期7卷 49-54页

作者： c. ScHMIDT j. ULRIcH center for Engineering Science Thermal Process Engineering Martin Luther University Halle-Wittenberg D-06099 Halle (Saale) Germany

The crystal morphology grown from a solution composed of an organic solvent, solute and additive can be predicted reliably by a computational method. Modeling the supersaturated solution as liquid phase is achieved by employing commercial software. The molecular composition of this solution is a required input parameter. The face specific diffusion coefficient of the solid （crystal surface） and liquid （solution） system is determined using the molecular dynamics procedure. The obtained diffusion coefficient is related to the specific face growth rate via the attachment energy of the pure morphology. The significant improvements are achieved in the morphology prediction because the investigation on the face growth rates in a complex growth environment （as multi-component solutions with additives） can be carried out based on the diffusion coefficients.

关键词： crystallization morphology molecular dynamics solution

Electro-osmotic flow of a second-grade fluid in a porous microchannel subj.ct to an Ac electric field

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j.urnal of Hydrodynamics 2013年第2期25卷 309-316页

作者： MISRA j. c. cHANDRA S. Department of Mathematics Institute of Technical Education and Research Siksha O Anusandhan University Department of Physics Sabang S. K. Mahavidyalaya Vidyasagar University

Studies on electro-osmotic flows of various types of fluids in microchannel are of great importance owing to their multifold applications in the transport of liquids, particularly when the ionized liquid flows with respect to a charged surface in the presence of an external electric field. In the case of viscoelastic fluids, the volumetric flow rate differs significantly from that of Newtonian fluids, even when the flow takes place under the same pressure gradient and the same electric field. With this end in view, this paper is devoted to a study concerning the flow pattern of an electro-osmotic flow in a porous microchannel, which is under the action of an alternating electric field. The influence of various rheological and electro-osmotic parameters, e.g., the Reynolds number, Debye-Huckel parameter, shape factor and fluid viscoelasticity on the kinematics of the fluid, has been investigated for a secondgrade viscoelastic fluid. The problem is first treated by using analytical methods, but the quantitative estimates are obtained numerically with the help of the software MATHEMATIcA. The results presented here are applicable to the cases where the channel height is much greater than the thickness of the electrical double layer comprising the Stern and diffuse layers. The study reveals that a larger value of the Debye-Huckel parameter creates sharper profile near the wall and also that the velocity of electro-osmotic flow increases as the permeability of the porous microchannel is enhanced. The study further shows that the electro-osmotic flow dominates at lower values of Reynolds number. The results presented here will be quite useful to validate the observations of experimental investigations on the characteristics of electro-osmotic flows and also the results of complex numerical models that are necessary to deal with more realistic situations, where electro-osmotic flows come into the picture, as in blood flow in the micro-circulatory system subj.ct to an elect

关键词： electrical double layer Debye length second-grade fluid Ionic energy

Sodium lanthanide fluoride core-shell nanocrystals： A general perspective on epitaxial shell growth

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Nano Research 2013年第8期6卷 547-561页

作者： Noah j. j. j.hnson Frank c. j. M. van Veggel Department of chemistry University ofVictoria P.O. Box3065 Victoria British Columbia Canada V8W 3V6

Understanding the structural characteristics and growth mechanism（s） are essen-tial for generating core-shell nano-heterostructures with distinctive properties. Especially in lanthanide-based nanocrystals, rational design of the core-shell composition can be utilized to enhance/tune the optical properties of the final nanostructure, or can be used to integrate multiple functional applications （e.g., luminescent/magnetic）. In this article, we review the progress in our current understanding of the epitaxial shell growth in sodium lanthanide fluoride （NaLnF4） nanocrystals. In order to understand epitaxial shell growth the core nanocrystals have to be uniform, and to date the synthesis of high quality near uniform size/shape dispersion controlled synthesis of lanthanide-based nanocrystals has been achieved mainly with this class of nanocrystals. The progress in core-shell synthesis and the epitaxial shell growth mechanism in this class of nanocrystals （NaLnF4） are reviewed, and a general perspective is provided on the core-shell morphology based on different characterization techniques. While there has been tremendous progress in studying the impact of core-shell structures in various functional applications, this review also highlights, in our view, the still limited understanding of ways to control the core-shell morphology and it emphasizes some important, unanswered questions that remain to be addressed to maximize their performance.

关键词： upconvertingnanocrystals core-shell lanthanides epitaxy

Molecular Frame Photoemission： Probe of the Photoionization Dynamics for Molecules in the Gas Phase

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chinese j.urnal of chemical Physics 2009年第2期22卷 178-186页

作者： D. Dowek Y. j. Picard P. Billaud c. Elkharrat j. c. Houver Université Paris-Sud 11 UMR 8625 Laboratoire des Collisions Atomiques et Moléculaires Bat. 351 Université Paris-Sud 91405 Orsay France cNRS UMR 8625 Laboratoire des Collisions Atomiques et Moléculaires Bat. 351 Université Paris-5ud 91405 Orsay France

Molecular frame photoemission is a very sensitive probe of the photoionization （PI） dynamics of molecules. This paper reports a comparative study of non-resonant and resonant photoionization of D2 induced by VUV circularly polarized synchrotron radiation at SOLEIL at the level of the molecular frame photoelectron angular distributions （MFPADs）. We use the vector correlation method which combines imaging and time-of-flight resolved electron-ion coincidence techniques, and a generalized formalism for the expression of the Ⅰ（χ, θe, Фe） MFPADs, where χ is the orientation of the molecular axis with respect to the light quantization axis and （θe, Фe） the electron emission direction in the molecular frame. Selected MFPADs for a molecule aligned parallel or perpendicular to linearly polarized light, or perpendicular to the propagation axis of circularly polarized light, are presented for dissociative photoionization （DPI） of D2 at two photon excitation energies, hv=19 eV, where direct PI is the only channel opened, and hv=32.5 eV, i.e. in the region involving resonant excitation of Q1 and Q2 doubly excited state series. We discuss in particular the properties of the circular dichroism characterizing photoemission in the molecular frame for direct and resonant PI. In the latter case, a remarkable behavior is observed which may be attributed to the interference occurring between undistinguishable autoionization decay channels.

关键词： Dissociative photoionization Molecular frame photoemission circular dichroism in photoemission Autoionization dynamics Synchrotron radiation Vector correlation