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Using oligonucleotide suspension arrays for laboratory identification of bacteria responsible for bacteremia

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j.urnal of Zhej.ang University-Science B(Biomedicine & Biotechnology) 2008年第4期9卷 291-298页

作者： Xiao-li HOU Han-liang j.ANG Qing-yi CAO Li-ying ZHAO Barbara j. chang Zhi CHEN Institute of Infectious Diseases the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China Department of Microbiology and Immunology School of Biomedical Biomolecular and Chemical Sciences University of Western Australia Nedlands WA 6009 Australia

The aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in Chinese hospitals. The multiplexed array, based on the QIAGEN LiquiChip Workstation, included 15 oligonucleotide probes which were covalently bound to different bead sets. PCR amplicons of a variable region of the bacterial 23S rRNA genes were hybridized to the bead-bound probes. Thirty-eight strains belonging to 15 species were correctly identified on the basis of their corresponding species-specific hybridization profiles. The results show that the suspension array, in a single assay, can differentiate isolates over a wide range of strains and species, and suggest the potential utility of suspension array system to clinical laboratory diagnosis.

关键词： Oligonucleotide array Bacteremia 23S rRNA Multiplexed detection

Generalized polynomial chaos expansion by reanalysis using static condensation based on substructuring

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Applied Mathematics and Mechanics(English Edition) 2024年第5期45卷 819-836页

作者： D.LEE S.chang j.LEE Department of Automotive Engineering Hanyang UniversitySeoul 04763Republic of Korea School of Mechanical Engineering Chungnam National UniversityDaejeon 34134Republic of Korea Department of Mechanical Robotics and Energy EngineeringDongguk UniversitySeoul 04620Republic of Korea

This paper presents a new computational method for forward uncertainty quantification(UQ)analyses on large-scale structural systems in the presence of arbitrary and dependent random *** method consists of a generalized polynomial chaos expansion(GPCE)for statistical moment and reliability analyses associated with the stochastic output and a static reanalysis method to generate the input-output data *** the reanalysis,we employ substructuring for a structure to isolate its local regions that vary due to random *** allows for avoiding repeated computations of invariant substructures while generating the input-output data *** substructuring with static condensation further improves the computational efficiency of the reanalysis without losing ***,the GPCE with the static reanalysis method can achieve significant computational saving,thus mitigating the curse of dimensionality to some degree for UQ under high-dimensional *** numerical results obtained from a simple structure indicate that the proposed method for UQ produces accurate solutions more efficiently than the GPCE using full finite element analyses(FEAs).We also demonstrate the efficiency and scalability of the proposed method by executing UQ for a large-scale wing-box structure under ten-dimensional(all-dependent)random inputs.

关键词： forward uncertainty quantification(UQ) generalized polynomial chaos expansion(GPCE) static reanalysis method static condensation substructuring

Using Microgripper in Development of Automatic Adhesive Glue Transferring and Binding Microassembly System

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Engineering（科研） 2010年第1期2卷 1-11页

作者： R. j. chang C. C. CHEN 不详

A system using microgripper for gluing and adhesive bonding in automatic microassembly was designed, implemented, and tested. The development of system is guided by axiomatic design principle. With a compliant PU microgripper, regional-edge-statistics (RES) algorithm, and PD controller, a visual-servoing system was implemented for gripping micro obj.ct, gluing adhesive, and operating adhesive bonding. The RES algorithm estimated and tracked a gripper’s centroid to implement a visual-servoing control in the microassembly operation. The main specifications of the system are: gripping range of 60~80μm, working space of 7mm×5.74mm×15mm, system bandwidth of 15Hz. In the performance test, a copper rod with diameter 60μm was automatically gripped and transported for transferring glue and bonding. The 60μm copper rod was dipped into a glue container and moved, pressed and bonding to a copper rod of 380μm. The amount of binding glue was estimated about 5.7nl.

关键词： Micro Gripper Adhesive Bonding Microassembly Visual Servo

Wide Absorption Spectrum Measuring Methods by DFB-LDs in Water Vapor Detection System

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Photonic Sensors 2014年第3期4卷 230-235页

作者： Y. N. LIU j. chang j. LIAN Q. WANG G. P. LV W. j. WANG Z. LIU X. Z. LIU S. S. ZHANG Z. L. WANG S. j.ANG C. G. ZHU W. WEI B.N. SUN School of Information Science and Engineering and Shandong Provincial Key Laboratory of Laser Technology andApplication Shandong University Jinan 250100 China Institute of Automation Shandong Academy of Sciences Jinan 250014 China

Two measuring methods of the wide absorption spectrum by distributed feedback laser diodes （DFB-LDs） are presented in detecting the water vapor absorption line. One is the subsection scanning method, and it takes advantage of the wide spectrum tuning range by the temperature modulation and fast spectrum tuning speed by current modulation. Specifically, this method is realized by dividing a target spectral region into several sections which correspond to the specific temperature of DFB-LD, scanning every section by current modulation for hundreds times, and averaging the data to raise the signal to noise ratio （SNR）, then combining all sections to get the whole spectrum. An accuracy of 10ppmv had been obtained in the measurement of water vapor with a 10-cm path length by this method. Another is data fitting method, based on the absorption line-shape function; the absorption line can be described by fitting with partial measured data. The fitting absorption line was fitted well with the measured data, and the square of correlation coefficient （R-square） was no less than 0.99.

关键词： DFB-LD wide spectrum subsection scanning data fitting water vapor detection

Effect of heat treatment on microstructure and mechanical properties of selective laser-melted PH13-8Mo stainless steel

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j.urnal of Iron and Steel Research International 2024年第4期31卷 945-955页

作者： Yuan Zhang Xiang Liu chang-j.n Wang chang Liu School of Material Science and Engineering Kunming University of Science and TechnologyKunming 650093YunnanChina Central Iron&Steel Research Institute Co. Ltd.Beijing 100081China

PH13-8Mo stainless steel powder with high sphericity and well fluidity was prepared by the plasma rotating electrode *** formed parts with ultra-high purity were manufactured by selective laser *** tensile and impact mechanical properties of the printed parts under three heat treatment regimes were compared to those without heat *** microstructure,grain orientation and phase composition were characterized by electron-backscatter diffraction,X-ray diffraction and transmission electron *** characterization results reveal that the addition of heat treatment makes the grains enlarge,and after different heat treatments,the average grain size from 1.51μm in the printed state increases to 2.78,3.09 and 2.06μm,*** formed parts are mainly composed of martensite and retained ***,the NiAl and M23C precipitates form,which are the maj.r strengthening phases of PH13-8Mo stainless *** optimal heat treatment process is 925℃×1 h water cooling(WC)+0℃×2 h air cooling(AC)+540℃×4 h *** this heat treatment process,PH13-8Mo formed parts have the optimal comprehensive mechanical properties:the tensile strength,yield strength and impact energy KU2 are 1492 MPa,1432 MPa and 63 j.*** strengthening and toughening via heat treatment are mainly attributed to dislocation strengthening,the formation of NiAl and M23Co precipitates and the change of volume fraction of the retained austenite.

关键词： High-strength stainless steel-Selective laser melting-Heat treatment Microstructure Mechanical property

On the Late Hydrothermal Tungsten Deposits of Haiyangshan Mountain, Kuangsi

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地质学报 1949年第Z1期 135-144页

作者： C. K.KUNG and j. H. chang (Geological Survey of China)

INTRODUCTIONThe Haiyangshan Mountain trending in the NNE-SSW direction is situated between the Hsiangan and Kuanyang cites in NE Kuangsi. The principal mines are located on the E slope at about 200 m above Sanyutsun, a village nearly 25 km NW of Kuanyang. The tungsten mineralization in the region is confined chiefly to the quartz veins which were formed by the ore-bearing solutions along the fractures in the granodiorite shortly after its consolidation.

关键词： Kuangsi On the Late Hydrothermal Tungsten Deposits of Haiyangshan Mountain

Addressable structured light system using metasurface optics and an individually addressable VCSEL array

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Photonics Research 2024年第6期12卷 1129-1137页

作者： CHENYANG WU XUANLUN HUANG YIPENG j. TINGYU CHENG j.AXING WANG NAN CHI SHAOHUA YU CONNIE j.chang-HASNAIN School of Information Science and Technology Fudan UniversityShanghai 200433China Berxel Photonics Co. Ltd.Shenzhen 518071China Peng Cheng Laboratory Shenzhen 518055China Chinese University of Hong Kong Shenzhen 518172China

Structured-light(SL)based 3D sensors have been widely used in many *** SL is the most widely deployed among all SL sensors due to its light weight,compact size,fast video rate,and low *** transmitter(known as the dot proj.ctor)consists of a randomly patterned vertical-cavity surface-emitting laser(VCSEL)array multiplicated by a diffractive optical element(DOE)with a fixed repeated *** that the separation of any two speckles is only one known and fixed number(albeit random),there are no other known scales to cal-ibrate or ***,typical SL sensors require extensive in-factory calibrations,and the depth resolution is limited to 1 mm at-60 cm *** this paper,to the best of our knowledge,we propose a novel dot proj.ctor and a new addressable SL(ASL)3D sensor by using a regularly spaced,individually addressable VCSEL array,multiplicated by a metasurface-DOE(MDOE)into a random pattern of the *** turning on or off the VCSELs in the array provides multiple known distances between neighboring speckles,which is used as a“built-in caliper”to achieve higher accuracy of *** as a precise“vernier caliper,”the addressable VCSEL array enables fine control over speckle positions and high detection *** experimentally dem-onstrated that the proposed method can result in sub-hundred-micron level *** new concept opens new possibilities for applications such as 3D computation,facial recognition,and wearable devices.

关键词： speckle light surface

Cell replacement with stem cell-derived retinal ganglion cells from different protocols

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Neural Regeneration Research 2024年第4期19卷 807-810页

作者： Ziming Luo Kun-Che chang Spencer Center for Vision Research Byers Eye InstituteStanford University School of Medicine Department of Ophthalmology Louis J.Fox Center for Vision RestorationUniversity of Pittsburgh School of Medicine Department of Neurobiology Center of NeuroscienceUniversity of Pittsburgh School of Medicine

Glaucoma,characterized by a degenerative loss of retinal ganglion cells,is the second leading cause of blindness *** is currently no cure for vision loss in glaucoma because retinal ganglion cells do not regenerate and are not replaced after *** stem cell-derived retinal ganglion cell transplant is a potential therapeutic strategy for retinal ganglion cell degenerative *** this review,we first discuss a 2D protocol for retinal ganglion cell differentiation from human stem cell culture,including a rapid protocol that can generate retinal ganglion cells in less than two weeks and focus on their transplantation ***,we discuss using 3D retinal organoids for retinal ganglion cell transplantation,comparing cell suspensions and *** review provides insight into current knowledge on human stem cell-derived retinal ganglion cell differentiation and transplantation,with an impact on the field of regenerative medicine and especially retinal ganglion cell degenerative diseases such as glaucoma and other optic neuropathies.

关键词： cell clumps cell suspension cell transplantation differentiation direct-induced protocol glaucoma optic neuropathy regenerative medicine retinal ganglion cell retinal organoids stem cells

THE SYNTHESIS OF PROTECTIVE FRAGMENTS OF α-HUMAN ATRIAL NATRIURETIC POLYPEPTIDE (α-hANP)

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Science in China,Ser.B 1988年第4期31卷 444-456页

作者：蔡孟深彭师奇程铁明戴敦华王少农李金祥张京莉丛阳梁伟升王融杨生生陈双环吴小东张艾卓 j.K.chang Department of Organic Chemistry Beij.ng Medical University

In this paper the synthesis of α-human atrial natriuretic polypeptide fragments (positions, 1-4, 5-10, 11-16, 17-22, 23-28) by solution method is reported. In the synthesis of some peptides, the re-sults from mixed anhydride procedure and active ester procedure are compared. In the synthesis of otherpeptides, the effects of reaction temperature, basicity of catalyst, and reaction time on the specific rota-tions and yields of these compounds are observed. The sequence of amino acids of all the protective poly-peptides is confirmed by FAB-MS.

关键词： Gly Cys HUMAN ATRIAL NATRIURETIC POLYPEPTIDE THE SYNTHESIS OF PROTECTIVE FRAGMENTS OF hANP BOC