Using oligonucleotide suspension arrays for laboratory identification of bacteria responsible for bacteremia

作     者：Xiao-li HOU Han-liang JIANG Qing-yi CAO Li-ying ZHAO Barbara J. CHANG Zhi CHEN 

作者机构：Institute of Infectious Diseases the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China Department of Microbiology and Immunology School of Biomedical Biomolecular and Chemical Sciences University of Western Australia Nedlands WA 6009 Australia 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2008年第9卷第4期

页      面：291-298页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：Project (Nos. 2003C13015 and 021103128) supported by Scienceand Technology Department of Zhejiang Province  China 

主　　题：Oligonucleotide array Bacteremia 23S rRNA Multiplexed detection 

摘      要：The aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in Chinese hospitals. The multiplexed array, based on the QIAGEN LiquiChip Workstation, included 15 oligonucleotide probes which were covalently bound to different bead sets. PCR amplicons of a variable region of the bacterial 23S rRNA genes were hybridized to the bead-bound probes. Thirty-eight strains belonging to 15 species were correctly identified on the basis of their corresponding species-specific hybridization profiles. The results show that the suspension array, in a single assay, can differentiate isolates over a wide range of strains and species, and suggest the potential utility of suspension array system to clinical laboratory diagnosis.