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Helicobacter pylori vacA s1a and s1b alleles from clinical isolates from different regions of Chile show a distinct geographic distribution

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World journal of Gastroenterology 2005年第40期11卷 6366-6372页

作者： MI Díaz A Valdivia P Martínez jL Palacios P Harris j Novales E Garrido D Valderrama C Shilling A Kirberg E Hebel j fierro R Bravo F Siegel G Leon G Klapp A Venegas Departamento de Genética Molecular y Microbiología. Pontificia Universidad Católica de Chile. Santiago Departamento de Pediatría Centro de Diagnóstico Terapéutico Los Angeles Unidad de Gastroenterología Anatomía Patológica Centro de Endoscopía Quirúrgica Hospital Base de Linares Hospital Regional de Valdivia Instituto de Bioquímica Hospital Regional de Punta Arenas

AIM: To establish the most common vacA alleles in Helicobacter pylori(H pylori) strains isolated from Chilean patients and its relationship with gastritis and gastroduodenal ulcers.METHODS: Two hundred and forty five H pylori clinical isolates were obtained from 79 biopsies from Chilean infected patients suffering from gastrointestinal diseases. An average of 2-3 strains per patient was isolated and the vacA genotype was analyzed by PCR and 3% agarose electrophoresis. Some genotypes were checked by DNA sequencing.RESULTS: The most prevalent vacA genotype inChilean patients was s1b m1 (76%), followed by s1a m1 (21%). In contrast, the s2 m2 genotype was scarcely represented (3%).The s1b m1 genotype was found most frequently linked to gastropathies (P＜0.05) rather than ulcers. Ulcers were found more commonly in male and older patients. Curiously, patients living in cities located North and far South of Santiago, the capital and largest Chilean city, carried almost exclusively strains with the s1b m1 genotype. In contrast, patients from Santiago and cities located South of Santiago carried strains with either one or both s1a m1 and s1b m1 genotypes.Regarding the s2 m2 genotype, comparison with GenBank sequences revealed that Chilean s2 sequence was identical to those of Australian, American, and Colombian strains but quite different from those of Alaska and India.CONCLUSION: Differences in geographic distribution of the s and m vaccA alleles in Chile and a relationship of s1b m1 genotype with gastritis were found. Sequence data in part support a hispanic origin for the vacA genotype.Asymmetric distribution of genotypes s1b m1 and s2 m2recedes H Pyloristrain distribution in Spain and Portugal.

关键词：地理分布智利幽门螺杆菌单离体

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美国的医疗事故

美国的医疗事故

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法庭科学最新技术研讨培训班

作者： Robert j.fierro FACOG MSHA Richmond Virginia MD

雅典的Solon(公元前约638-559)提出了公民可以有控告其他人错误行为的权利。美国的法律系统是从英国法律系统和法国其他有重要影响意义事件派生出来的,因此,自从殖民时代以后,美国的法律允许病人控告他的医生。在开始讨论美国医生的医...

雅典的Solon(公元前约638-559)提出了公民可以有控告其他人错误行为的权利。美国的法律系统是从英国法律系统和法国其他有重要影响意义事件派生出来的,因此,自从殖民时代以后,美国的法律允许病人控告他的医生。在开始讨论美国医生的医疗事故状况之前,有必要简单地回顾一下美国的法律系统。美国有50 个州,每个州都有他们自己的立法机关和司法系统,州法律由自己的立法机关制定,本州的案件由本

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强奸和性骚扰

强奸和性骚扰

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法庭科学最新技术研讨培训班

作者： Robert j.fierro,MD FACOG MSHA Richmond Virginia

＜正＞对于强奸和性骚扰的理解存在一些误区,强奸或性骚扰的受害者都是受到陌生人暴力被迫发生性行为的妇女。事实上性骚扰有多种形式,可以使暴力的也可能是非暴力的,受害人可能是男性或女性,可能是孩子或成年人,而罪犯可能是陌生人,也...

＜正＞对于强奸和性骚扰的理解存在一些误区,强奸或性骚扰的受害者都是受到陌生人暴力被迫发生性行为的妇女。事实上性骚扰有多种形式,可以使暴力的也可能是非暴力的,受害人可能是男性或女性,可能是孩子或成年人,而罪犯可能是陌生人,也可能是亲属、朋友、有地位的人或配偶。性骚扰在法律上的定义应当为:未经同意或以暴力进行的性行为。被害人（男或女）在暴力或其他诸如威胁、恐吓等形式下被迫发生性行为。

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污秽地区绝缘子串的在线监测

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新疆电力 1999年第2期 50-56页

作者： j. L. fierro-chavez 韩小地

位于墨西哥东北部地区４００ｋＶ线路的外绝缘，由于污秽积累，已我次闪络。为保证线路安全云霞我种措施。目前已研制出测试绝缘子泄漏电流及表面电阻的系统以帮助这些线路进行维护，该系统已安装在几基杆塔上，作为监测污秽绝缘子串表... 详细信息

位于墨西哥东北部地区４００ｋＶ线路的外绝缘，由于污秽积累，已我次闪络。为保证线路安全云霞我种措施。目前已研制出测试绝缘子泄漏电流及表面电阻的系统以帮助这些线路进行维护，该系统已安装在几基杆塔上，作为监测污秽绝缘子串表面状况的诊断工具，本文叙述了该系统的开发、实验室测试及现场安装。

关键词：绝缘子串在线监测污秽地区输电线路

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Clinical Factors Associated with Hepatocellular Iron Deposition in End-stage Liver Disease

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journal of Clinical and Translational Hepatology 2020年第3期8卷 231-239页

作者： Amelia fierro-Fine Leana Guerin Hasan Hicsasmaz Kyle E.Brown Department of Pathology University of Iowa Roy J.and Lucille A.Carver College of MedicineIowa CityIAUSA ObjectRelational LLP Iowa CityIAUSA Division of Gastroenterology-Hepatology University of Iowa Roy J.and Lucille A.Carver College of MedicineIowa CityIAUSA Program in Free Radical and Radiation Biology University of Iowa Roy J.and Lucille A.Carver College of MedicineIowa CityIAUSA Iowa City Veterans Administration Medical Center Iowa CityIAUSA

Background and Aims: Hepatocellular iron accumulation in patients with chronic liver disease has been linked to adverse outcomes. The objective of this study was to identify clinical factors associated with hemosiderosis. Methods: A total of 103 consecutive liver transplant recipients were identified, in whom liver biopsy had been performed prior to transplantation. Laboratory and clinical data at biopsy and transplant were abstracted from the medical records and hepatocyte iron was graded in the biopsy and explant. The association of change in iron score from biopsy to transplant, with the time interval between these two events, was examined using linear mixed model analysis for repeated measures. Results: Most subjects had advanced fibrosis (F3-F4) at liver biopsy, which was performed on average about 2.5 years before transplant. Over 80% of patients had no or 1+ hepatocyte iron at biopsy;iron increased between biopsy and transplant in about 40%. The only demographic or clinical feature that correlated with increased iron was the presence of a transjugular intrahepatic portosystemic shunt. Increased iron at transplant was associated with higher serum iron and transferrin saturation at biopsy, and with lower hemoglobin level, greater mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration, higher ferritin and model for end-stage liver disease score at transplant.Conclusions: The development of hemosiderosis in end-stage liver disease is associated with lower hemoglobin levels and alterations in red blood cell indices that are suggestive of hemolysis. These observations suggest that extravascular hemolysis may play a role in the development of secondary iron overload.

关键词： Erythrocyte indices End-stage liver disease Hemolysis Hemosiderosis Humans Iron

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Participation of Auxin Transport in the Early Response of the Arabidopsis Root System to Inoculation with Azospirillum brasilense

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Phyton-International journal of Experimental Botany 2022年第11期91卷 2383-2401页

作者： Elizabeth Carrillo-Flores jonanci Arreola-Rivera DenníMariana Pazos-Solís Moisés Bocanegra-Mondragón Grisel fierro-Romero MaElena Mellado-Rojas Elda Beltrán-Peña Laboratorio de Transducción de Señales Edificio B3Instituto de Investigaciones Químico-Biológicas de la Universidad Michoacana de San Nicolás de HidalgoCiudad UniversitariaMorelia58030México Tecnológico de Monterrey School of Engineering and SciencesCampus QuerétaroSan PabloQuerétaro76130México School of the Environment Florida A&M UniversityTallahassee32307USA

The potential of Plant Growth Promoting Rhizobacteria(PGPR)has been demonstrated in the case of plant inoculation with bacteria of the genus Azospirillum which improves yield.A.brasilense produces a wide variety of molecules,including the natural auxin indole-3-acetic acid(IAA),as well as other phytoregulators.However,several studies have suggested that auxin induces changes in plant development during their interaction with the bacteria.The effects of A.brasilense Sp245 on the development of Arabidopsis thaliana root were investigated to help explain the molecular basis of the interaction.The results obtained showed a decrease in primary root length from the first day and remained so throughout the exposure,accompanied by a stimulation of initiation and maturation of lateral root primordia and an increase of lateral roots.An enhanced auxin response was evident in the vascular tissue and lateral root meristems of inoculated plants.However,after five days of bacterization,the response disappeared in the primary root meristems.The role of polar auxin transport(PAT)in auxins relocation involved the PGP1,AXR4-1,and BEN2 proteins,which apparently mediated A.brasilense-induced root branching of Arabidopsis seedlings.

关键词： Auxin Azopirillum brasilense Arabidopsis auxin transport lateral roots

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Pronuclear formation by ICSI using chemically activated ovine oocytes and zona pellucida bound sperm

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journal of Animal Science and Biotechnology 2017年第2期8卷 370-377页

作者： j.E.Hernández-Pichardo Y.Ducolomb S.Romo M.E.Kjelland R.fierro F.Casillas M.Betancourt División de Ciencias Biológicas y de la Salud Departamento de Producción Agrícola y AnimalUniversidad Autónoma Metropolitana-XochimilcoCiudad de MexicoMexico Division de Ciencias Biologicas y de la Salud Departamento de Ciencias de la SaludUniversidad Autónoma Metropolitana-IztapalapaSan Rafael Atlixco 186CP 09340 Ciudad de MexicoMexico Departamento de Ciencias Pecuarias Facultad de EstudiosSuperiores CuautitlanUNAMCiudad de MexicoEstado de MexicoMexico Conservation Genetics&BiotechLLCValley CityNDUSA Division de Ciencias Biologicas y de la Salud Departamento de Biologia de la ReproduccionUniversidad Autonoma Metropolitana-lztapalapaCiudad de MexicoMexico Doctorado en Biologia Experimental Universidad Autonoma Metropolitana-lztapalapaCiudad de MexicoMexico

Background： In order to improve ICSI, appropiate sperm selection and oocyte activation is necessary. The objective of the present study was to determine the efficiency of fertilization using ICSI with chemically activated ovine oocytes and sperm selected by swim up（SU） or swim up ＋ zona pellucida（SU ＋ ZP） binding.Results： Experiment 1, 4–20 replicates with total 821 in vitro matured oocytes were chemically activated with ethanol, calcium ionophore or ionomycin, to determine oocyte activation（precense of one PN）. Treatments showed similar results（54, 47, 42 %, respectively） but statistically differents（P 0.05）.Conclusions： Chemical activation induces higher ovine oocyte activation than mechanical activation. Ethanol slightly displays higher oocyte activation than calcium ionophore and ionomicine. Sperm selection with SU ＋ ZP increased AR/A and AR/D rates in comparison with SU in fresh and frozen-thawed sperm. According to this, in terms of fertilization rates, chemical activation after ICSI increased oocyte PN formation compared to mechanical activation. Also, fresh sperm treated with SU and SU ＋ ZP were significantly different than frozen-thawed sperm,but between sperm treatments no significant differences were obtained.

关键词： Ethanol ICSI Oocyte activation Ovine Pronucleus Zona pellucida

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Developing targets for radiation transport experiments at the Omega laser facility

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High Power Laser Science and Engineering 2017年第3期5卷 1-4页

作者： D.Capelli C.A.Charsley-Groffman R.B.Randolph D.W.Schmidt T.Cardenas F.fierro G.Rivera C.Hamilton j.D.Hager H.M.johns N.E.Lanier j.L.Kline Los Alamos National Laboratory

Targets have been developed to measure supersonic radiation transport in aerogel foams using absorption spectroscopy.The target consists of an aerogel foam uniformly doped with either titanium or scandium inserted into an undoped aerogel foam package. This creates a localized doped foam region to provide spatial resolution for the measurement.Development and characterization of the foams is a key challenge in addition to machining and assembling the two foams so they mate without gaps. The foam package is inserted into a beryllium sleeve and mounted on a gold hohlraum.The target is mounted to a holder created using additive manufacturing and mounted on a stalk. The manufacturing of the components, along with assembly and metrology of the target are described here.

关键词： absorption spectroscopy doped foams radiation transport

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Catalytic Performance of La0.6Ca0.4Fe1-xNixO3Perovskites in DME Oxidation

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Modern Research in Catalysis 2015年第4期4卷 97-106页

作者： Gina Pecchi Ruddy Morales Daniela Salinas Eduardo j. Delgado josé Luis García fierro Facultad Ciencias Químicas Universidad de Concepción Concepción Chile Instituo Catálisisy Petroleoquímica CSIC Cantoblanco Madrid Spain

Substituted La0.6Ca0.4Fe1-xNixO3 (0.0 ≤ x ≤ 1.0) perovskite-type oxides prepared by the amorphous citrate method have been investigated as catalysts in the dimethyl ether (DME) oxidation reaction. The samples with an x ≤ 0.3 composition show an orthorhombic structure, while the intermediate compositions with 0.5 ≤ x ≤ 0.9 show a less crystalline orthorhombic perovskite structure with segregated phases that increase upon increasing the Ni content. The progressive substitution of Fe3+ by Ni2+ in La0.6Ca0.4FeO3 entails the progressive formation of oxygen vacancies and Fe4+ species for Ni substitution degrees of x ≤ 0.5. For larger degrees of substitution with x > 0.7, segregated phases and a progressive loss of the perovskite structure are detected. The highest catalytic performance for the total combustion of DME is obtained from equimolar substitution in the B-site position (Fe0.5Ni0.5), in which a large extent of Fe4+ species and oxygen vacancies are present.

关键词： DME Perovskites Oxidation Iron Nickel

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Assemblage-based biomonitoring of freshwater ecosystem health via multimetric indices:A critical review and suggestions for improving their applicability

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Water Biology and Security 2022年第3期1卷 28-42页

作者： Robert L.Vadas jr. Robert M.Hughes Yeon jae Bae Min jeong Baek Orestes Carlos Bello Gonzáles Marcos Callisto Débora ReisdeCarvalho Kai Chen Maria T.Ferreira Pablo fierro jon S.Harding Dana M.Infante C.j.Kleynhans Diego R.Macedo Isabela Martins Norman Mercado Silva Nabor Moya Susan j.Nichols Paulo S.Pompeu Renata Ruaro Deborah R.O.Silva R.jan Stevenson Bianca de Freitas Terra Christa Thirion Douglas Ticiani Lizhu Wang Chris O.Yoder 2909 Boulevard Rd SEOlympiaWA98501-3971USA Amnis Opes Institute 2895 SE GlennCorvallisOR97333USA Department of Fisheries Wildlife&Conservation SciencesOregon State UniversityCorvallisOR97331USA Division of Environmental Science&Ecological Engineering Korea University145 Anam-roSeongbuk-guSeoul02841South Korea National Institute of Biological Resources Ministry of Environment42 Hwangyeong-roSeo-guIncheon22689South Korea Centro de Investigacion en Biodiversidad y Conservacion Universidad Autonoma del Estado de Morelos1001Col.ChamilpaC.P62209CuernavacaMorelosMexico Departamento de Genética Ecologia e EvoluçaoInstituto de Ciências BiologicaUniversidade Federal de Minas GeraisAvenida Ant^onio Carlos 6627CEP31270-901Belo HorizonteMGBrazil Departamento de Ecologia e Conservaçao Universidade Federal de Lavras37200-900LavrasMGBrazil State Key Laboratory of Marine Resource Utiliization in South China Sea Hainan UniversityHaikou570228China Forest Research Centre&Associate Laboratory TERRA University of LisbonTapada da Ajuda1349-017LisbonPortugal Instituto de Ciencias Marinas y Limnologicas Universidad Austral de ChileValdiviaChile School of Biological Sciences University of CanterburyPrivate Bag 4800Christchurch8140New Zealand Department of Fisheries&Wildlife Michigan State University1405 South Harrison Road#318East LansingMI48823USA Department of Water Affairs and Forestry(retired) 8th Avenue 771Wonderboom South0084PretoriaSouth Africa Departamento de Geografia Instituto de GeociênciasUniversidade Federal de Minas GeraisAvenida Antônio Carlos 6627CEP31270-901Belo HorizonteMGBrazil Instituto Guaicuy 109 Rua BrasopolisBelo HorizonteMG31150-170Brazil Centro de Investigación en Biodiversidad y Conservación Universidad Autónoma del Estado de MorelosAvenida Universidad 1001Col.ChamilpaC.P.62209CuernavacaMorelosMexico Instituto Experimental de Biología Universidad San Francisco Xavier de ChuquisacaSucreBolivia Centre for Applied Water Science Un

Freshwater biota are more comprehensive and direct indicators of biological impacts,and more meaningful to the public than water quality or physical habitat surrogates.Freshwater biotic data and the multiple biological indicators developed from them offer a much richer array of data for assessing the impacts of pollution controls than a limited set of physical or chemical measures.In recent decades,assemblage-based assessments by ecologists,environmental scientists,and water quality agencies have been employed globally for determining the condition of,and threats to,freshwater ecosystems.A key step in this advance has been the development of multimetric indices(MMIs)or indices of biotic integrity(IBIs)based on quantitative assessments of algae,macrophyte,macroinvertebrate,fish or riparian bird assemblages.In Europe,where biological assemblages are mandated for assessing freshwater ecosystem health,many indices are multimetric.However,the proliferation of MMIs globally has not always occurred through the application of rigorous study designs and monitoring protocols,nor have they always effectively incorporated functional metrics,stressor assessments,and statistical analyses.Therefore,in this review,we discuss eleven major concerns with the development and application(including logistical limitations)of multimetric indicators based on freshwater biota to encourage more rigorous and widely applicable(transferable)MMI use and implementation.Specifically,our concerns focus on reference conditions;sampling effort,methods,and season;trophic guild definition;metric comprehensiveness,options,screening and scoring;and MMI validation.MMIs could also benefit from increased attention to ecological mechanisms and metric development,to further improve our understanding of anthropogenic impacts as well as rehabilitation effects on freshwater ecosystems globally.Paying closer attention to study designs,ecological mechanisms and metric development should further improve our understanding of anthropogenic impacts and better facili

关键词： IBI MMI Fish Macroinvertebrates Algae Streams Rivers Lakes Wetlands Biological monitoring

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