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Investigation of human organoid retina with digital holographic transmission matrix measurements

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Light(Advanced Manufacturing) 2022年第2期3卷 33-47页

作者： Nektarios Koukourakis Felix wagner Stefan Rothe Mike O.Karl jürgen w.czarske Laboratory for Measurement and Sensorsystem Technique TU DresdenDresdenGermany Competence Center for Biomedical Laser Systems(BIOLAS) TU DresdenDresdenGermany Center for Regenerative Therapies Dresden(CRTD) TU DresdenDresdenGermany German Center for Neurodegenerative Diseases(DZNE)Dresden DresdenGermany Faculty of Physics TU DresdenDresdenGermany Excellence Cluster Physics of Life(PoL) TU DresdenDresdenGermany

Advanced manufacturing of retinal organoid samples from human induced pluripotent stem cells represents a promising way to study the development of retinal *** retina is an epithelium composed of different cell layers with unique optical properties and detects light by photoreceptor neurons for visual *** are still many challenges in detecting early and distinct cellular changes in retinal *** this paper,we study the capability of the optical transmission matrix,which fully describes the transition of a light field propagating through a scattering *** its rich information content,the transmission matrix is commonly just used for light delivery through scattering *** holography is employed to measure the complex light-field information of the transmitted *** demonstrate that singular value decomposition of the transmission matrix allows to discriminate phantom tissues with varying scattering *** apply these findings to retinal organoid *** of the protonophore carbonyl cyanide m-chloro-phenylhydrazone(CCCP),a known inducer of retinal damage in animals,caused cell death and structural changes in human retinal organoids,which resulted in distinct changes in the transmission *** data indicate that the analysis of the transmission matrix can distinguish pathologic changes of the retina towards the development of imaging-based biomarkers.

关键词： Transmission matrix Digital holography Retina Organoids Neurodegeneration Cell death

Ultra-thin 3D lensless fiber endoscopy using diffractive optical elements and deep neural networks

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Light(Advanced Manufacturing) 2021年第4期2卷 57-66页

作者： Robert Kuschmierz Elias Scharf David F.Ortegón-González Tom Glosemeyer jürgen w.czarske Laboratory of Measurement and Sensor System Technique TU DresdenHelmholtzstrasse 1801069 DresdenGermany Competence center for Biomedical Computational Laser Systems(BIOLAS) TU DresdenGermany Institute of Applied Physics School of SciencesTU DresdenGermany Excellence Cluster Physics of Life TU DresdenGermany

Minimally invasive endoscopes are indispensable in *** fiber bundles(CFBs)enable ultrathin lensless ***,the propagation of light through a CFB suffers from phase distortions and aberrations that can cause images to be *** correction of such aberrations has been demonstrated using various techniques for wavefront control,especially using spatial light modulators(SLMs).This study investigates a novel aberration correction without SLM for the creation of an efficient and compact *** memory effect of CFBs enables a paradigm shift in the use of static diffractive optical elements(DOEs)instead of dynamic modulation with *** introduce DOEs produced by 2-photon polymerization lithography for phase conjugation on a CFB for focusing,raster scanning,and ***,a DOE with random patterns is used to encode the three-dimensional(3D)object information in a 2D speckle pattern that propagates along the ultra-thin *** networks decode the speckles to retrieve the 3D object information using single-shot *** DOE methods have compact low-cost concepts in common,and both pave the way for minimally invasive 3D endomicroscopy with benefits for optical imaging in biomedicine.

关键词： correction speckle elements

Compressive holographic sensing simplifies quantitative phase imaging

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Light(Science & Applications) 2023年第6期12卷 946-948页

作者： jiawei Sun juergen w.czarske Competence Center for Biomedical Computational Laser Systems(BIOLAS) TU DresdenDresdenGermany Laboratory of Measurement and Sensor System Technique(MST) TU DresdenHelmholtzstrasse 1801069 DresdenGermany Cluster of Excellence Physics of Life TU DresdenDresdenGermany Institute of Applied Physics TU DresdenDresdenGermany

Quantitative phase imaging (QPI) has emerged as method for investigating biological specimen and technical objects. However, conventional methods often suffer from shortcomings in image quality, such as the twin image artifact. A novel computational framework for QPI is presented with high quality inline holographic imaging from a single intensity image. This paradigm shift is promising for advanced QPI of cells and tissues.

关键词： holographic phase shortcomings

Lensless fiber endomicroscopy in biomedicine

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PhotoniX 2024年第1期5卷 402-410页

作者： jiawei Sun Robert Kuschmierz Ori Katz Nektarios Koukourakis juergen w.czarske Competence Center for Biomedical Computational Laser Systems(BIOLAS) TU DresdenDresden 01069Germany Shanghai Artificial Intelligence Laboratory Longwen Road 129Xuhui DistrictShanghai 200232China Laboratory of Measurement and Sensor System Technique(MST) TU DresdenHelmholtzstrasse 18Dresden 01069Germany Else Kroner Fresenius Center for digital Heath TU DresdenDresden 01062Germany Institute of Applied Physics The Hebrew University of JerusalemJerusalemIsrael Cluster of Excellence Physics of Life TU DresdenDresden 01062Germany Institute of Applied Physics TU DresdenDresden 01062Germany

Lensless fiber endomicroscopy,an emergent paradigm shift for minimally-invasive microscopic optical imaging and targeted light delivery,holds transformative potential,especially in *** holographic detection and physical or computational wavefront correction,it enables three-dimensional imaging in an unprecedentedly small footprint,which is crucial for various applications such as brain *** perspective reviews the recent breakthroughs,highlighting potential emerging applications,and pinpointing gaps between innovation and real-world *** the research in this realm accelerates,the novel breakthroughs and existing frontiers highlighted in this perspective can be used as guidelines for researchers joining this exciting domain.

关键词： Lensless endomicroscope Biomedical imaging Multimode fibers Multicore fibers Translation to real-world applications

Quantitative phase imaging through an ultra-thin lensless fiber endoscope

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Light(Science & Applications) 2022年第8期11卷 1843-1852页

作者： jiawei Sun jiachen wu Song wu Ruchi Goswami Salvatore Girardo Liangcai Cao jochen Guck Nektarios Koukourakis juergen w.czarske Laboratory of Measurement and Sensor System Technique(MST) TU DresdenHelmholtzstrasse 1801069DresdenGermany Competence Center for Biomedical Computational Laser Systems(BIOLAS) TU DresdenDresdenGermany State Key Laboratory of Precision Measurement Technology and Instruments Department of Precision InstrumentsTsinghua University100084BeijingChina Institute for Integrative Nanosciences IFW DresdenHelmholtzstraße 2001069DresdenGermany Max Planck Institute for the Science of Light&Max-Planck-Zentrum für Physik und Medizin 91058ErlangenGermany Cluster of Excellence Physics of Life TU DresdenDresdenGermany Institute of Applied Physics TU DresdenDresdenGermany

Quantitative phase imaging(QPI)is a label-free technique providing both morphology and quantitative biophysical information in ***,applying such a powerful technique to in vivo pathological diagnosis remains ***-core fiber bundles(MCFs)enable ultra-thin probes for in vivo imaging,but current MCF imaging techniques are limited to amplitude imaging *** demonstrate a computational lensless microendoscope that uses an ultra-thin bare MCF to perform quantitative phase imaging with microscale lateral resolution and nanoscale axial sensitivity of the optical path *** incident complex light field at the measurement side is precisely reconstructed from the far-field speckle pattern at the detection side,enabling digital refocusing in a multi-layer sample without any mechanical *** accuracy of the quantitative phase reconstruction is validated by imaging the phase target and hydrogel beads through the *** the proposed imaging modality,three-dimensional imaging of human cancer cells is achieved through the ultra-thin fiber endoscope,promising widespread clinical applications.

关键词： fiber phase thin