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Evaluation of total protein,peroxidase,and nutrients measured by pXRF for the determination of tissue rejuvenation/reinvigoration of Eucalyptus microcorys

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林业研究:英文版 2023年第5期34卷 1563-1576页

作者： júlio c.zar tannure faria caius Ribeiro-Kumara William Macedo Delarmelina Filipe Aiura Namorato Dione Richer Momolli Anderson cleiton josé Enéas Ricardo Konzen Dulcinéia de carvalho Gilvano Ebling Brondani Department of Forestry and Wood Sciences Federal University of Espírito SantoJerônimo MonteiroEspírito SantoBrazil Department of Forest Sciences University of HelsinkiHelsinkiFinland Federal Institute of Education Science and Technology of Espírito SantoIbatibaEspírito SantoBrazil Department of Soil Science Federal University of LavrasLavrasMinas GeraisBrazil Department of Forest Engineering Federal University of Santa MariaSanta MariaRio Grande Do SulBrazil Department of Forestry Sciences Federal University of LavrasLavrasMinas GeraisBrazil Department Interdisciplinary Federal University of Rio Grande Do SulImbéRio Grande Do SulBrazil

Ontogenetic aging of tissues and the gradual decrease of adventitious rooting are known challenges for the clonal propagation of woody species,hampering clonal forestry *** study examined possible signatures of tissue rejuvenation/reinvigoration in different propagated materials of Eucalyptus microcorys by analyzing the total protein profile,peroxidase activity,macro-and micronutrient contents,and adventitious rooting of mini *** analyses were performed on *** shoots which were successfully obtained by seminal and grafting propagation,micropropagation with epicormic shoots,and indirect *** four mature trees used in the propagation,tissues from the one with the best propagation results were investigated for signs of tissue rejuvenation and/or *** individuals from each technique were randomly selected and transferred to a semi-hydroponic"channel"*** four weeks in the seedbed,the total protein,peroxidase activity,nutrient content and rooting of the mini cuttings were ***-PAGE enabled the differentiation of leaf samples obtained by grafting from the other propagation techniques,as revealed by two distinct *** obtained by micropropagation with epicormic shoots showed the highest peroxidase activity,while those obtained by seminal propagation and from the selected mature tree showed the lowest peroxidase activity.A portable X-ray fluorescence spectroscope(pXRF)identified adequate nutrient content in most of the nutrients tested in materials obtained by seminal and grafting propagation,and by indirect *** analysis of adventitious rooting showed that the highest rooting percentage was observed in mini cuttings from seminal propagation(75%)followed by indirect organogenesis(35%).Based on principal component analysis,it was concluded that rooting of mini cuttings from both seminal propagation and indirect organogenesis was associated with phosphorous,sulphur,and potassium

关键词： Adventitious rooting juvenility Reinvigoration Rejuvenation Ontogenetic age

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Effects of chemical sterilization of the culture media,porous membranes and luminosity on in vitro culture of Eucalyptus grandis×Eucalyptus urophylla

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journal of Forestry Research 2021年第4期32卷 1587-1598页

作者： Letícia Vaz Molinari Denys Matheus Santana costa Souza Maria Lopes Martins Avelar Sérgio Bruno Fernandes Douglas Santos Gonçalves júlio c.zar tannure faria Dulcineia de carvalho Gilvano Ebling Brondani Department of Forestry Sciences Federal University of LavrasLavrasMinas GeraisBrazil

Hybrid combinations of Eucalyptus have increased due to expansion of plantations into unconventional areas and to the search for higher quality ***,most of these species have difficulties surviving in vitro *** chlorine and sealing systems are often used to reduce contamination and increase gas *** aim of the present study is to evaluate the establishment,multiplication,elongation and adventitious rooting of *** × *** clones(c1 and c2) and four active chlorine concentrations(0.000%,0.001%,0.003%,and 0.005%) were tested in the establishment and multiplication *** sealing forms(W/M,1/M and 3/M) and the same four active chlorine concentrations were applied to the elongation *** luminosities(dark and light)and three sealings(W/M,1/M and 3/M) were tested during adventitious *** chlorine concentration of0.005% led to the lowest fungal contamination rate and to the highest in vitro *** chlorine concentration of 0.003% resulted in the greatest length and highest number of shoots per explant in the multiplication *** were no phytotoxicity problems and the quality of plants grown in an environment with active chlorine was maintained in comparison with those grown in an *** increase in gas exchange in ventilation systems had a positive impact on the in vitro growth and development of plants.

关键词： Asepsis cloning Gas exchange In vitro propagation

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correction to:Effects of chemical sterilization of the culture media,porous membranes and luminosity on in vitro culture of Eucalyptus grandis×Eucalyptus urophylla

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journal of Forestry Research 2022年第5期33卷 1705-1707页

correction to:***://***/10.1007/s11676-020-01240-5 In the original publication of the article,there were errors in the fig 3 and fig *** corrected Figs.3 and 6 are given below:The original article has been corrected.

关键词： corrected porous media

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Evaluation of total protein,peroxidase,and nutrients measured by pXRF for the determination of tissue rejuvenation/reinvigoration of Eucalyptus microcorys

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林业研究（英文版） 2023年第5期

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