Effects of chemical sterilization of the culture media,porous membranes and luminosity on in vitro culture of Eucalyptus grandis×Eucalyptus urophylla

作     者：Letícia Vaz Molinari Denys Matheus Santana Costa Souza Maria Lopes Martins Avelar Sérgio Bruno Fernandes Douglas Santos Gonçalves Júlio Cézar Tannure Faria Dulcineia de Carvalho Gilvano Ebling Brondani Letícia Vaz Molinari;Denys Matheus Santana Costa Souza;Maria Lopes Martins Avelar;Sérgio Bruno Fernandes;Douglas Santos Gon?alves;Júlio Cézar Tannure Faria;Dulcineia de Carvalho;Gilvano Ebling Brondani

作者机构：Department of Forestry SciencesFederal University of LavrasLavrasMinas GeraisBrazil 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2021年第32卷第4期

页      面：1587-1598页

核心收录：

学科分类：0907[农学-林学] 08[工学] 0829[工学-林业工程] 09[农学] 

基　　金：the support of the Coordination for the Improvement of Higher Education Personnel-Brazil (CAPES) the National Council for Scientific and Technological Development (CNPq) the Research Support Foundation of the State of Minas (FAPEMIG) 

主　　题：Asepsis Cloning Gas exchange In vitro propagation 

摘      要：Hybrid combinations of Eucalyptus have increased due to expansion of plantations into unconventional areas and to the search for higher quality ***,most of these species have difficulties surviving in vitro *** chlorine and sealing systems are often used to reduce contamination and increase gas *** aim of the present study is to evaluate the establishment,multiplication,elongation and adventitious rooting of *** × *** clones(C1 and C2) and four active chlorine concentrations(0.000%,0.001%,0.003%,and 0.005%) were tested in the establishment and multiplication *** sealing forms(W/M,1/M and 3/M) and the same four active chlorine concentrations were applied to the elongation *** luminosities(dark and light)and three sealings(W/M,1/M and 3/M) were tested during adventitious *** chlorine concentration of0.005% led to the lowest fungal contamination rate and to the highest in vitro *** chlorine concentration of 0.003% resulted in the greatest length and highest number of shoots per explant in the multiplication *** were no phytotoxicity problems and the quality of plants grown in an environment with active chlorine was maintained in comparison with those grown in an *** increase in gas exchange in ventilation systems had a positive impact on the in vitro growth and development of plants.