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Increased expression of PELP1 in human sperm is correlated with decreased semen quality

Asian Journal of Andrology 2018年第5期20卷 425-431页

作者： izabela skibinska Miroslaw Andrusiewicz Michal Soin Magdalena Jendraszak Paulina Urbaniak Piotr Jedrzejczak Malgorzata Kotwicka Department of Cell Biology Faculty of Health Sciences Poznan University of Medical Sciences Rokietnicka 5D Poznan 60-806 Poland Division of Infertility and Reproductive Endocrinology Faculty of Medicine I Poznan University of Medical Sciences Polna 33 Poznan 60-535 Poland.

Proline-, glutamic acid-, and leucine-rich protein 1 （PELP1） is a scaffolding protein involved in both genomic and nongenomic estrogen signal transduction pathways. To date, the role of PELP1 protein has yet to be characterized in human sperm and has not been associated with sperm parameters. To confirm the presence of PELP1 in human sperm, fresh semen samples were obtained from 178 donors. The study was designed to establish both mRNA and protein presence, and protein cellular localization. Additionally, the number of PELP1-positive spermatozoa was analyzed in men with normal and abnormal semen parameters. Sperm parameters were assessed according to the World Health Organization （WHO） 2010 standards. The presence of PELP1 in spermatozoa was investigated using four precise, independent techniques. The qualitative presence of transcripts and protein was assessed using reverse transcription-polymerase chain reaction （RT-PCR） and western blot protocols, respectively. The cellular localization of PELP1 was investigated by immunocytochemistry. Quantitative analysis of PELP1-positive cells was done by flow cytometry. PELP1 mRNA and protein was confirmed in spermatozoa. Immunocytochemical analysis identified the presence of PELP1 in the midpieces of human sperm irrespective of sperm parameters. Becton Dickinson fluorescence-activated cell sorting （FACSCalibur^Tm） analysis revealed a significantly lower number of PELP1-positive cells in males with normal semen parameters versus abnormal samples （42.78% ± 11.77% vs 61.05% ± 21.70%, respectively; P = 0.014）. The assessment of PELP1 may be a time-saving method used to obtain information about sperm quality. The results of our study suggest that PEPL1 may be utilized as an indicator of sperm quality; thereby, PELP1 may be an additional biomarker useful in the evaluation of male infertility.

关键词： male infertility proline glutamic acid- and leucine-rich protein 1 semen parameters sperm quality spermatozoa

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Wide-angle digital holography with aliasing-free recording

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Photonics Research 2024年第5期12卷 1098-1106页

作者： RAFAL KUKOLOWICZ izabela GEREJ TOMASZ KOZACKI Institute of Micromechanics and Photonics Warsaw University of Technology02-525 WarsawPoland

High-quality wide-angle holographic content is at the heart of the success of near-eye display *** work proposes the first digital holographic(DH)system enabling recording wide-angle scenes assembled from objects larger than the setup field of view(FOV),which can be directly replayed without 3D deformation in the near-eye *** hologram formation in the DH system comprises free space propagation and Fourier transform(FT),which are connected by a rectangular ***,the object wave propagates in free space to the rectangular ***,the band-limited wavefield is propagated through the single lens toward the camera *** rectangular aperture can take two sizes,depending on which DH operates in off-axis or phase-shifting recording *** integral part of the DH solution is a numerical reconstruction algorithm consisting of two elements:fringe processing for object wave recovery and wide-angle propagation to the object *** second element simulates propagation through both parts of the experimental *** free space part is a space-limited angular spectrum compact space algorithm,while for propagation through the lens,the piecewise FT algorithm with Petzval curvature compensation is *** the experimental part of the paper,we present the wide-angle DH system with FOV25°×19°,which allows high-quality recording and reconstruction of large complex scenes.

关键词： recording angle holographic

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Rescue of p53 functions by in vitro-transcribed mRNA impedes the growth of high-grade serous ovarian cancer

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Cancer Communications 2024年第1期44卷 101-126页

作者： Monika Raab izabela Kostova Samuel Peña-Llopis Daniela Fietz Monika Kressin Seyed Mohsen Aberoumandi Evelyn Ullrich Sven Becker Mourad Sanhaji Klaus Strebhardt Department of Gynecology Medical SchoolGoethe-UniversityFrankfurt am MainGermany Translational Genomics in Solid Tumors West German Cancer CenterUniversity HospitalEssenGermany German Cancer Consortium(DKTK) EssenGermany German Cancer Research Center(DKFZ) HeidelbergGermany Histology and Embryology Institute for Veterinary AnatomyGiessenGermany Franfurt Cancer Institute(FCI) Goethe UniversityFrankfurt am MainGermany German Cancer Consortium(DKTK) Partner site Frankfurt/Mainza partnership between DKFZ and University Hospital FrankfurtFrankfurt am MainGermany Experimental Immunology Department for Children and Adolescents MedicineUniversity Hospital FrankfurtGoethe UniversityFrankfurt am MainGermany

Background:The cellular tumor protein p53(TP53)is a tumor suppressor gene that is frequently mutated in human *** various cancer types,the very aggressive high-grade serous ovarian carcinoma(HGSOC)exhibits the high-est prevalence of TP53 mutations,present in>96%of *** intensive efforts to reactivate p53,no clinical drug has been approved to rescue p53 *** this study,our primary objective was to administer in vitro-transcribed(IVT)wild-type(WT)p53-mRNA to HGSOC cell lines,primary cells,and ortho-topic mouse models,with the aim of exploring its impact on inhibiting tumor growth and dissemination,both in vitro and in ***:To restore the activity of p53,WT p53 was exogenously expressed in HGSOC cell lines using a mammalian vector ***,IVT WT p53 mRNA was delivered into different HGSOC model systems(primary cells and patient-derived organoids)using liposomes and studied for proliferation,cell cycle progression,apoptosis,colony formation,and chromosomal *** alterations induced by p53 mRNA were analyzed using RNA sequencing in OVCAR-8 and primary HGSOC cells,followed by ingenuity path-way *** vivo effects on tumor growth and metastasis were studied using orthotopic xenografts and metastatic intraperitoneal mouse ***:Reactivation of the TP53 tumor suppressor gene was explored in differ-ent HGSOC model systems using newly designed IVT mRNA-based *** introduction of WT p53 mRNA triggered dose-dependent apoptosis,cell cycle arrest,and potent long-lasting inhibition of HGSOC cell *** analysis of OVCAR-8 cells upon mRNA-based p53 reactivation revealed significant alterations in gene expression related to p53 signaling,such as apoptosis,cell cycle regulation,and DNA *** p53 function concurrently reduces chromosomal instability within the HGSOC cells,under-scoring its crucial contribution in safeguarding genomic integrity by moderating the baselin

关键词： apoptosis cell cycle chromosomal instability HGSOC metastatic intraperitoneal mouse model HGSOC orthotopic Xenograft model high-grade serous ovarian cancer in vitro-transcribed p53-mRNA liposomal IVT mRNA delivery system patient-derived organoid patient-derived primary cancer cell

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Interactions of naturally occurring compounds with antimicrobials

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Journal of Pharmaceutical Analysis 2023年第12期13卷 1452-1470页

作者： izabela Malczak Anna Gajda Department of Pharmacology and Toxicology National Veterinary Research InstitutePartyzantów 5724-100Poland

Antibiotics are among the most often used medications in human healthcare and *** these substances can lead to complications such as increasing antibiotic resistance in bacteria or a toxic effect when administering large *** solve these problems,new solutions in antibacterial therapy are *** use of natural products in medicine has been known for *** of them have antibacterial activity,hence the idea to combine their activity with commercial antibiotics to reduce the latter's *** review presents collected information on natural compounds(terpenes,alkaloids,flavonoids,tannins,sulfoxides,and mycotoxins),of which various drug interactions have been *** of the indicated compounds show synergistic or additive interactions with antibiotics,which suggests their potential for use in antibacterial therapy,reducing the toxicity of the antibiotics used and the risk of further development of bacterial ***,there are also compounds which interact antagonistically,potentially hindering the therapy of bacterial *** on its mechanism of action,each compound can behave differently in combination with different antibiotics and when acting against various bacterial strains.

关键词： Interactions Antimicrobials Natural compounds Antibiotics

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Is hydrogen peroxide generated in wine?

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Food Bioscience 2022年第1期45卷 393-396页

作者： Anna Tama Grzegorz Bartosz izabela Sadowska-Bartosz Laboratory of Analytical Biochemistry Institute of Food Technology and NutritionCollege of Natural SciencesRzeszow University35-601RzeszowPoland Department of Bioenergetics Food Analysis and MicrobiologyInstitute of Food Technology and NutritionCollege of Natural SciencesRzeszow University35-601RzeszowPoland

Wines contain significant amounts of polyphenols which autoxidize upon contact with oxygen,producing hydrogen *** checked more than 40 samples of white and red wines finding,on average,production of small amounts of hydrogen peroxide within 3 h after the wines had contacted with air,though some samples did not produce detectable amounts of H_(2)O_(2).The increase in hydrogen peroxide concentration after 3-h incubation of wines after opening ranged from -3.09 to 6.60μ*** showed also the ability of hydrogen peroxide scavenging,which can be partly attributed to ***,the measured net production of hydrogen peroxide in wine is a result of the actual production and scavenging of hydrogen peroxide.

关键词： Wine Hydrogen peroxide Sulfite Polyphenols Autoxidation

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Probiotics and postbiotics in colorectal cancer:Prevention and complementary therapy

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World Journal of Gastroenterology 2022年第27期28卷 3370-3382页

作者： Monika Kvakova Anna Kamlarova Jana Stofilova Veronika Benetinova izabela Bertkova Center of Clinical and Preclinical Research MEDIPARK Faculty of MedicineP.J.Safarik University in KosiceKosice 04011Slovakia

Colorectal cancer(CRC)is a leading cause of human mortality *** conventional anticancer therapy not always being effective,there is growing interest in innovative“drug-free”cancer treatments or interventions that improve the efficacy of established *** is associated with microbiome alterations,a process known as dysbiosis that involves depletion and/or enrichment of particular gut bacterial species and their metabolic *** patient treatment with traditional probiotics(with or without prebiotics),nextgeneration probiotics(NGP),or postbiotics represents a potentially effective and accessible complementary anticancer strategy by restoring gut microbiota composition and/or by signaling to the *** this capacity,restoration of the gut microbiota in cancer patients can stabilize and enhance intestinal barrier function,as well as promote anticarcinogenic,anti-inflammatory,antimutagenic or other biologically important biochemical pathways that show high specificity towards tumor *** benefits of traditional probiotics,NGP,and postbiotics include modulating gut microbiota composition and function,as well as the host inflammatory *** application in CRC prevention is highlighted in this review,where we consider supportive in vitro,animal,and clinical *** on emerging research,NGP and postbiotics hold promise in establishing innovative treatments for CRC by conferring physiological functions via the production of dominant natural products and metabolites that provide new host-microbiota signals to combat *** favorable results have been reported,further investigations focusing on strain and dose specificity are required to ensure the efficacy and safety of traditional probiotics,NGP,and postbiotics in CRC prevention and treatment.

关键词： Colorectal cancer Traditional probiotics Next-generation probiotics Postbiotics Gut microbiota

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Legends of the dashboard: an empirical evaluation of split and joint layout designs for geovisual analytics interfaces

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International Journal of Digital Earth 2023年第1期16卷 1395-1417页

作者： izabela Gołębiowska Tomasz Opach ArzuÇöltekin Jolanta Korycka-Skorupa Jan Ketil Rød Faculty of Geography and Regional Studies University of WarsawWarsawPoland Department of Geography Norwegian University of Science and TechnologyTrondheimNorway Institute for Interactive Technologies University of Applied Sciences and Arts Northwestern SwitzerlandWindischSwitzerland

Geodashboards are often designed with explanatory elements guiding *** elements(*** or annotations)need to be carefully designed to mitigate split attention or information integration *** this paper,we report expert interviews followed by a controlled experiment where we compare two interface designs with a focus on the split attention effect:(1)a multiple-legend layout with explanatory elements located next to each view,and(2)a single-legend layout with all explanatory elements gathered in one *** legend layouts did not affect the performance,but affected user satisfaction.75%of the participants preferred the multiple-legend layout,and rated it with a higher usability score,mainly attributing this preference to the proximity of legend elements to the view of *** tracking data strongly and clearly verifies that participants indeed make use of the proximity:With the single-legend,the majority of eye-movement transitions were between the single-legend and the closest view to the legend,whereas with multiple-legend participants have shorter and more frequent legend visits,as well as more transitions between legends and *** together,the design lesson we learned from this experiment can be summarized as‘split the legend elements,but make it close to the explained elements’.

关键词： Geodashboard layout design legend split attention user study eye tracking

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Intraspecific Variability and Problematic Dimorphism in the Early Cretaceous(Valanginian)Ammonite Saynoceras verrucosum(d'Orbigny,1841)

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Acta Geologica Sinica(English Edition) 2007年第6期81卷 877-882页

作者： izabela PLOCH Polish Geological Institute Geological Museum

The population of Saynoceras verrucosum （d＇Orbigny） from the Polish basin （Wawal section, central Poland） shows no significant intraspecific variability. Dimorphism has not been found in this population. Statistical analyses of material from the shallow epicratonic Polish basin and the relatively deep Vocontian basin （southeastern France） indicated two populations. Forms from the Polish basin are somewhat more inflated and smaller than forms from the Vocontian basin.

关键词： intraspecific variation Saynoceras verrucosum dimorphism Central Poland Wawal Section

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Venous and arterial thromboembolism in patients with inflammatory bowel diseases

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World Journal of Gastroenterology 2021年第40期27卷 6757-6774页

作者： Antoni Stadnicki izabela Stadnicka Department of Physiology Faculty of MedicineUniversity of TechnologyKatowice 41-209Poland Department of Molecular Medicine Medical University of SilesiaFaculty of PharmacySosnowiec 41-200Poland

The risk of thromboembolism(TE)is increased in patients with inflammatory bowel disease(IBD),mainly due to an increased risk of venous TE(VTE).The risk of arterial TE(ATE)is less pronounced,but an increased risk of cardiovascular diseases needs to be addressed in IBD *** predisposes to arterial and venous thrombosis through similar prothrombotic mechanisms,including triggering activation of coagulation,in part mediated by impairment of the intestinal barrier and released bacterial *** in IBD has clinical specificities,i.e.,an earlier first episode in life,high rates during both active and remission stages,higher recurrence rates,and poor *** increased likelihood of VTE in IBD patients may be related to surgery,the use of medications such as corticosteroids or tofacitinib,whereas infliximab is ***-term complications of VTE can include post-thrombotic syndrome and high recurrence rate during post-hospital discharge.A global clot lysis assay may be useful in identifying patients with IBD who are at risk for *** VTEs occur in IBD outpatients;therefore,outpatient prophylaxis in high-risk patients is *** is crucial to continue focusing on prevention and adequate treatment of VTE in patients with IBD.

关键词： Inflammatory bowel disease Thrombosis Coagulation Bacterial components Prophylaxis Post-thrombotic syndrome

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Cathepsin L, transmembrane peptidase/serine subfamily member 2/4, and other host proteases in COVID-19 pathogenesis – with impact on gastrointestinal tract

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World Journal of Gastroenterology 2021年第39期27卷 6590-6600页

作者： izabela Berdowska Malgorzata Matusiewicz Department of Biochemistry and Immunochemistry Wroclaw Medical UniversityWroclaw 50-368Lower SilesiaPoland

Severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)seems to employ two routes of entrance to the host cell;via membrane fusion(with the cells expressing both angiotensin converting enzyme 2(ACE2)and transmembrane peptidase/serine subfamily member 2/4(TMPRSS2/4))or via receptor-mediated endocytosis(to the target cells expressing only ACE2).The second mode is associated with cysteine cathepsins(probably cathepsin L)involvement in the virus spike protein(S protein)proteolytic *** furin might activate the virus S protein enabling it to enter *** tract(GIT)involvement in SARS-CoV-2 infection is evident in a subset of coronavirus disease 2019(COVID-19)patients exhibiting GIT symptoms,such as diarrhea,and presenting viral-shedding in *** the abundance and co-localization of ACE2 and TMPRSS2 in the lower GIT(especially brush-border enterocytes),these two receptors seem to be mainly involved in SARS-CoV-2 invasion of the digestive ***,in vitro studies have demonstrated the virions capability of infection and replication in the human epithelial cells lining ***,also furin and cysteine cathepsins(cathepsin L)might participate in the activation of SARS-CoV-2 spike protein contributing to the virus invasiveness within ***,cathepsin L(due to its involvement in extracellular matrix components degradation and remodeling,the processes enhanced during SARS-CoV-2-induced inflammation)might be responsible for the dysregulation of absorption/digestion functions of GIT,thus adding to the observed in some COVID-19 patients symptoms such as diarrhea.

关键词： COVID-19 SARS-CoV-2 Angiotensin converting enzyme 2 Transmembrane peptidase/serine subfamily member 2/4 Cathepsin L Gastrointestinal tract

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