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Biochars and their magnetic derivatives as enzyme-like catalysts mimicking peroxidases

Biochar 2020年第1期2卷 121-134页

作者： ivo Safarik Jitka Prochazkova Eva Baldikova Hans-Peter Schmidt Witold Kwapinski ivo Medrik Petr Jakubec Mirka Safarikova Kristyna Pospiskova Department of Nanobiotechnology Biology CentreISBCASNa Sadkach 737005 Ceske BudejoviceCzech Republic Regional Centre of Advanced Technologies and Materials Palacky UniversitySlechtitelu 2778371 OlomoucCzech Republic Ithaka Institute for Carbon Strategies Ancienne Eglise 9ArbazSwitzerland Chemical Sciences Department Bernal InstituteFaculty of Science and EngineeringUniversity of LimerickLimerickIreland

Various materials have been extensively investigated to mimic the structures and functions of natural *** describe the discovery of a new catalytic property in the group of biochar-based carbonaceous materials,which are usually produced during biowaste thermal processing under specific *** tested biochars exhibited peroxidase-like catalytic *** feedstock,pyrolysis temperature,size of resulting biochar particles or biochar modification(e.g.,magnetic particles deposition)influenced the peroxidase-like *** activity was measured with the chromogenic organic substrates N,N-diethyl-p-phenylenediamine(DPD)or 3,3′,5,5′-tetramethylbenzidine(TMB),in the presence of hydrogen *** biochar composite was studied as a complementary material,in which the presence of iron oxide particles enhances catalytic activity and enables smart magnetic separation of catalyst even from complex *** activity of the selected biochar had an optimum at pH 4 and temperature 32℃;biochar catalyst can be reused ten times without the loss of *** DPD as a substrate,Km values for native wood chip biochar and its magnetic derivative were 220±5μmol L^(−1)and 690±80μmol L^(−1),respectively,while Vmax values were 10.1±0.3μmol L^(−1)min^(−1)and 16.1±0.4μmol L^(−1)min^(−1),*** catalytic activity enabled the decolorization of crystal violet both in the model solution and the fish pond water containing suspended solids and dissolved organic *** observed biochar enzyme mimetic activity can thus find interesting applications in environmental technology for the degradation of selected *** general,this property predestines the low-cost biochar to be a perspective supplement or even substitution of common peroxidases in practical applications.

关键词： Peroxidase-like activity Biochar Magnetic iron oxides Microwave synthesis

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Phosphoinositide and Inositolpolyphosphate Signalling in Defense Responses of Arabidopsis thaliana Challenged by Mechanical Wounding

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Molecular Plant 2008年第2期1卷 249-261页

作者： Alina Mosblech Sabine Konig Irene Stenzel Peter Grzeganek ivo Feussner Ingo Heilmann Department of Plant Biochemistry Albrecht-yon-Hailer-Institute for Plant Sciences Georg-August-University G6ttingen Justus-von-Liebig-Weg 1137077 G6ttingen Germany

Various biochemical signals are implicated in Arabidopsis wound signalling, including jasmonic acid （JA）, salicylic acid, auxin, and Ca^2＋. Here, we report on cross-talk of phytohormones with phosphoinositide signals not previously implicated in plant wound responses. Within 30 min of mechanical wounding of Arabidopsis rosette-leaves, the levels of the lipid-derived soluble inositolpolyphosphate, inositol 1,4,5-trisphosphate （InsP3）, increased four to five-fold. Concom- itantly, the precursor lipids, phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 4-phosphate and phosphatidylinositol transiently depleted, followed by re-synthesis after 30-60 min of stimulation. Increased InsP3 levels with wounding coincided with JA increases over the first hours of stimulation. In dde2-2-mutant plants deficient in JA biosynthesis, no InsP3 increase was observed upon wounding, indicating that JA was required for InsP3 formation, and InsP3 levels increased in wild-type plants challenged with sorbitol, increasing endogenous JA levels. In InsP 5-ptase plants with attenuated phosphoinositide signalling, the induction of wounding-inducible genes was diminished compared with wildtype plants, suggesting a role for phosphoinositide signalling in mediating plant wound responses. The gene-expression patterns suggest that phosphoinositides contribute to both JA-dependent and JA-independent aspects of wound signalling. Weight gain of Plutella xylostella caterpillars feeding on InsP 5-ptase plants was increased compared with that of caterpillars feeding on wild-type plants. The ecophysiological relevance of phosphoinositide signals in plant defense responses to herbivory is discussed in light of recent findings of inositolpolyphosphate involvement in phytohormone-receptor function.

关键词： Phosphoinositide and Inositolpolyphosphate Signalling Arabidopsis Mechanical Wounding

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Technosol made from coal waste as a strategy to plant growth and environmental control

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Energy Geoscience 2021年第2期2卷 160-166页

作者： Beatriz A.Firpo Jessica Weiler ivo A.H.Schneider Universidade Federal Do Rio Grande Do Sul LTM-DEMIN/PPGE3MAv.Bento Gonçalves 9500CEP:91501-970Porto AlegreRSBrazil

Coal waste disposal areas demand proper rehabilitation activities because several environmental impacts are related to them,such as acid mine drainage,loss of biohabitats,water pollution,and soil *** most common strategy is to cover them with an impermeable layer followed by a new soil layer as soon as possible,while maximizing plant growth and avoiding water and wind *** study examines the possibility of transforming coal waste itself into a substrate for plant growth,namely technosol,assuming its own use for progressive rehabilitation and revegetation of waste *** coal waste is amended with other residues in an integrated waste management approach:rice husk ash,steel slag,and sewage sludge to adjust physical structure,pH,and nutrient availability,*** raw material composition,fertility,metals bioavailability,plant growth,and nutrients in plant tissue are analyzed after successive growth of lopsided oats(Avena strigosa)and maize(Zea mays).The results show that coal waste allows a fertile plant substrate after being amended in accordance to experimental *** soil metal concentration is found to fall within the limits of natural variation for soils in the study area and nutrients in plant tissue are found to be consistent with lopsided oats and maize *** proposed waste mixture results in a technosol capable of adequately promoting plant growth,that is,it is a potential resource to accelerate revegetation and natural succession in coal waste disposal areas.

关键词： Mine waste Mine soil Technogenic soil Waste management Mine reclamation

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Rapid determination of iron oxide content in magnetically modified particulate materials

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Particuology 2016年第3期14卷 114-117页

作者： ivo Safarik Leona Nydlova Kristyna Pospiskova Eva Baldikova Zdenka Maderova Mirka Safarikova Department of Nanobiotechnology Institute of Nanobiology and Structural Biology ofGCRC Na Sadkach 7 370 05 Ceske Budejovice Czech Republic Regional Centre of Advanced Technologies and Materials Palacky University Slechtitelu 27 783 71 Olomouc Czech Republic Department of Nanobiotechnology Biology Centre CAS Na Sadkach 7 370 05 Ceske Budejovice Czech Republic Department of Applied Chemistry Faculty of Agriculture University of South Bohemia Branisovska 1457 370 05 Ceske Budejovice Czech Republic

Magnetically responsive composite materials have been used in interesting applications in various areas of bioscience, biotechnology, and environmental technology. In this work, a simple method to determine the amount of magnetic iron oxide nano- and microparticles attached to magnetically-modified partic- ulate diamagnetic materials has been developed using a commercially available magnetic permeability meter, The procedure is fast and enables dry particulate magnetically modified materials to be analysed without any modification or pretreatment. We show that the magnetic permeability can be measured for materials containing up to 20% magnetic iron oxide, The magnetic permeability measurements are highly reproducible.

关键词： Magnetic permeability meter Magnetic iron oxides Magnetically modified materials

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Targeted nanoparticles for the non-invasive detection of traumatic brain injury by optical imaging and fluorine magnetic resonance imaging

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Nano Research 2016年第5期9卷 1276-1289页

作者： Luis Javier Cruz ivo Que Markus Aswendt Alan Chan Mathias Hoehn Clemens Lowik Nanomedicine and Molecular Imaging Department of Radiology Leiden University Medical Center 2333ZA Leiden the Netherlands In-Vivo-NMR Laboratory Max Planck Institute for Neurological Research Cologne D-50931 Cologne Germany Percuros 7522NB Enschede the Netherlands

Necrosis is a form of cell death that occurs only under pathological conditions such as ischemic diseases and traumatic brain injury （TBI）. Non-invasive imaging of the affected tissue is a key component of novel therapeutic interventions and measurement of treatment responses in patients. Here, we report a bimodal approach for the detection and monitoring of TBI. PEGylated poly（lactic-coglycolic acid）（PLGA） nanoparticles （NPs）, encapsulating both near infrared （NIR） fluorophores and perfluorocarbons （PFCs）, were targeted to necrotic ceils. We used cyanine dyes such as IRDye 800CW, for which we have previously demonstrated specific targeting to intracellular proteins of cells that have lost membrane integrity. Here, we show specific in vivo detection of necrosis by optical imaging and fluorine magnetic resonance imaging （^19F MRI） using newly designed PLGA NP（NIR700 ＋ PFC）-PEG-800CW. Quantitative ex vivo optical imaging and ^19F MR spectroscopy of NIR-PFC content in injured brain regions and in major organs were well correlated. Both modalities allowed the in vivo identification of necrotic brain lesions in a mouse model of TBI, with optical imaging being more sensitive than ^19F MRI. Our results confirm increased blood pool residence time of PLGA NPs coated with a PEG layer and the successful targeting of TBI-damaged tissue. A single PLGA NP containing NIR-PFC enables both rapid qualitative optical monitoring of the TBI state and quantitative 3D information from deeper tissues on the extent of the lesion by MRI. These necrosis-targeting PLGA NPs can potentially be used for clinical diagnosis of brain injuries.

关键词： imaging nanoparticles contrast agents magnetic resonance imaging brain injury stroke

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Identifying more reliable parameters for the detection of change during the follow-up of mild to moderate keratoconus patients

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Eye and Vision 2017年第1期4卷 143-149页

作者： ivo Guber Colm McAlinden François Majo Ciara Bergin Department of Ophthalmology University of GenevaGenevaSwitzerland Department of Ophthalmology Glangwili HospitalHywel Dda University Health BoardCarmarthenUK School of Ophthalmology and Optometry Wenzhou Medical UniversityWenzhouZhejiangChina Jules-Gonin Eye Hospital University of LausanneFondation Asile des AveuglesLausanneSwitzerland

Background:Reaching a consensus on which parameters are most reliable at detecting progressive keratoconus patients with serial topography imaging is not *** aim of the study was to isolate the parameters best positioned to detect keratoconus progression using the Pentacam HR^(®)measures based on the respective limits of repeatability and range of ***:Using the Pentacam HR^(®),a tolerance index was calculated on anterior segment parameters in healthy and keratoconic *** tolerance index provides a scale from least to most affected parameters in terms of measurement noise relative to that observed in healthy ***,based on the“number of increments”from no disease to advanced disease,a relative utility(RU)score was also *** values close to 1 indicate parameters best positioned to detect a change in keratoconic ***:The tolerance index values indicated that 36%of ocular parameters for keratoconic eyes had repeatability limits which were wider than normative limits(worse),but 28%of the ocular parameters were narrower than normative limits(better).Considering only those parameters with a RU greater than 0.95,a small number of parameters were within this range,such as corneal curvature and asphericity ***:This study demonstrates that measurement error in keratoconic eyes is significantly greater than healthy *** implemented here provide guidance on the levels of expected precision in keratoconic eyes relative to healthy eyes to aid clinicians in distinguishing real change from *** maximal keratometry(Kmax),central corneal thickness(CCT)and thinnest corneal thickness(TCT)were highlighted as problematic indices for the follow-up of keratoconus in terms of repeatability.

关键词： Pentacam Keratoconus Progression Repeatability Precision Corneal crosslinking

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Resiliency oriented control of a smart microgrid with photovoltaic modules

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Global Energy Interconnection 2021年第5期4卷 441-452页

作者： Sambeet Mishra Kristjan Peterson Tauno Hilimon Jelena Shuvalova Fushuan Wen ivo Palu Department of Electrical Power Engineering and Mechatronics Tallinn University of Technology

The resiliency of a standalone microgrid is of considerable issue because the available regulation measures and capabilities are *** this background,this paper presented a new mathematical model for a detailed photovoltaic(PV)module and the application of new control techniques for efficient energy *** PV module employs a single-stage conversion method to integrate it with the utility *** extraction the maximum power from PV and integrate it to power grid,a three-phase voltage source converter is *** obtaining the maximum power at a particular irradiance a maximum power point tracking(MPPT)scheme is *** fuzzy logic control and adaptive network-based fuzzy inference system are proposed for direct current(DC)link voltage *** proposed model and control scheme are validated through a comparison with the standard power-voltage and current-voltage charts for a PV *** results demonstrate that the system stability can be maintained with the power grid and in the island mode,in contrast with the MPPT.

关键词： PV module MPPT Adaptive network-based fuzzy inference system(ANFIS) Converter control Grid integration

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Effects of a Food Supplement with a Wild Thyme (Thymus serpyllum L.) Extract on Gut Health and the Microbiome in Humans: A Randomized, Double-Blinded, Placebo-Controlled Clinical Trial

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Food and Nutrition Sciences 2022年第11期13卷 931-949页

作者： Katharina Knaub Christiane Schön Cynthia G. Suarez ivo Pischel BioTeSys GmbH Esslingen Germany Finzelberg GmbH & Co. KG Andernach Germany Dr. ivo Pischel Consulting Rossbach Germany

Background: Based on the scientific and traditional knowledge, benefits for gut and digestive health were expected from Wild Thyme (Thymus serpyllum L.) extract (WThE) consumption, but no controlled human studies were performed yet to prove the proposed health benefits. Method: The aim of this human randomized, double-blind parallel arm pilot study was to explore the impact of aqueous Wild Thyme extract, a food supplement, in a healthy but overweight study collective (N = 40). In detail, the impact on digestion, gastrointestinal symptoms, gut microbiome, and quality of life by employing an essential oil-free WThE preparation or a matching placebo was investigated. Results: The study results indicate that WThE has the potential to improve gastrointestinal symptoms and increase stool frequency, thus an improved quality of life was observed. The stool microbiome of study collective was characterized by a high Firmicutes to Bacteroidetes ratio. A decrease in the mean Firmicutes/Bacteroidetes ratio was seen in WThE group. Conclusion: The data support the potential applications of WThE as a food supplement with benefits on gut health.

关键词： Wild Thyme (Thymus serpyllum L.) Extract Gut Health Gut Microbiome Abdominal Comfort Bowel Movement Mental Well-Being Quality of Life

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A Rare Presentation of Erdheim-Chester Disease Overlaped with Langerhans Histiocytosis

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International Journal of Clinical Medicine 2023年第10期14卷 438-448页

作者： ivo De Menezes Filadélfia Martins Rosineli Oliveira Victor Meireles Felipe Nobre Carlos Alberto Studart Gomes Hospital Fortaleza Brazil

Erdheim-Chester Disease (ECD) is a rare condition and has various differential diagnoses with other forms of histiocytosis, classified as one of non-Langerhans histiocytosis. The diagnosis of this condition remains challenging because its presentation includes non-specific systemic manifestations that can affect different organs caused by deposition of lipids and fibrosis. Most common include bone pain followed by progressive weakness and different lung manifestations. This case is about a rare presentation of ECD with Langerhans Histiocytosis as overlap syndrome, with findings of both diseases in a middle aged woman that presented dyspnea as the first symptom. The patient was treated initially as heart failure and remained without any improvement, being admitted to investigate. After a stricted follow-up, bone and lung involvement were noticed and a skin biopsy unveiled xanthomatized macrophages accompanied by Touton giant cells. This condition remains an important clinical entity and should provide new insights for clinicians dealing with respiratory diseases.

关键词： Erdheim-Chester Langerhans Histicytosis Pulmonary Histiocytosis

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Islet amyloid polypeptide & amyloid beta peptide roles in Alzheimer’s disease:two triggers, one disease

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Neural Regeneration Research 2021年第6期16卷 1127-1130页

作者： Sofia Ferreira Ana F.Raimundo Regina Menezes ivo C.Martins iBET-Instituto de Biologia Experimental e Tecnológica OeirasPortugal CEDOC-Chronic Diseases Research Center Faculdade de Ciências MédicasUniversidade Nova de LisboaLisboaPortugal ITQB-NOVA Instituto de Tecnologia Química e Biológica António XavierUniversidade Nova de LisboaOeirasPortugal Instituto de Medicina Molecular Faculdade de MedicinaUniversidade de LisboaLisbonPortugal

Alzheimer’s disease(AD)is a neurodegenerative disorder that affects millions *** to population ageing,the incidence of AD is *** patients develop cognitive decline and dementia,features for which is known,requiring permanent *** poses a major socio-economic burden on healthcare systems as AD patients’relatives and healthcare workers are forced to cope with rising numbers of affected *** recent advances,AD pathological mechanisms are not fully ***,it is clear that the amyloid beta(Aβ)peptide,which forms amyloid plaques in AD patients’brains,plays a key *** 2 diabetes,the most common form of diabetes,affects hundreds of million people *** amyloid polypeptide(IAPP)is a hormone coproduced and secreted with insulin in pancreatic β-cells,with a key role in diabetes,as it helps regulate glucose levels and control adiposity and *** to Aβ,IAPP is very amyloidogenic,generating intracellular amyloid deposits that causeβ-cell dysfunction and *** is now clear that IAPP can also have a pathological role in AD,decreasing cognitive *** harms the blood-brain barrier,directly interacts and co-deposits with Aβ,promoting diabetes-associated *** can cause a metabolic dysfunction in the brain,leading to other diabetes-related forms of ***,here we discuss IAPP association with diabetes,Aβand dementia,in the context of what we designate a“diabetes brain phenotype”AD *** approach helps to set a conceptual framework for future IAPP-based drugs against AD.

关键词： aggregation Alzheimer amylin amyloid diabetes islet amyloid polypeptide

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