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New In Vitro Studies on the Bioprofile of Genista tenera Antihyperglycemic Extract

Natural Products and Bioprospecting 2015年第6期5卷 277-285页

作者： Daniela Batista Pedro L.Falé Maria L.Serralheiro Maria E.Araújo Paulo J.A.Madeira Carlos Borges isabel torgal Margarida Goulart Jorge Justino Alice Martins Amélia P.Rauter Centro de Química e Bioquímica Faculdade de CiênciasUniversidade de LisboaCampo Grande1749-016 LisbonPortugal Institute of Pharmaceutical Science King’s College London150 Stamford StreetLondon SE19NHUK Escola Superior Agrária de Santarém Quinta do Galinheiro2001-904 SantarémPortugal

The inhibition of a-glucosidase and glucose-6-phosphatase,two enzymes involved in the carbohydrate metabolism,is an important target to control glycaemia on individuals with type 2 *** this work we report for the first time the inhibition of both enzymes by the antihyperglycemic n-butanol extract from Genista tenera(Fabaceae).This extract decreased a-glucosidase and glucose-6-phosphatase activities to 0.97 and 80.25%,respectively,being more effective than acarbose,and phlorizin,the positive controls,which reduced enzymes activities only to 17.39 and 96.06%.Once inflammation and oxidative stress are related to diabetic impairments,the anti-inflammatory activity of the extract was also evaluated,through its inhibitory activity over COX-1 enzyme(47.5%inhibition).Moreover,after induction of oxidative stress by UV radiation,the viability of irradiated rat liver hepatoma cells exposed to the extract was significantly higher(67.82%)than that promoted by ascorbic acid,the positive control(45.05%).In addition,the stability of the extract under gastrointestinal conditions was evaluated by HPLC–DAD-ESI–MS/*** diglycosides were identified as the main constituents of the extract,and no alterations in the chemical composition nor in the antioxidant activity were observed after in vitro digestion with artificial gastric and pancreatic juices.

关键词： a-Glucosidase inhibition In vitro digestion HPLC-DAD-ESI-MS/MS Antioxidant activity Flavonoid glycosides

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