New In Vitro Studies on the Bioprofile of Genista tenera Antihyperglycemic Extract
作者机构：Centro de Química e BioquímicaFaculdade de CiênciasUniversidade de LisboaCampo Grande1749-016 LisbonPortugal Institute of Pharmaceutical ScienceKing’s College London150 Stamford StreetLondon SE19NHUK Escola Superior Agrária de SantarémQuinta do Galinheiro2001-904 SantarémPortugal
出 版 物：《Natural Products and Bioprospecting》 (应用天然产物（英文）)
年 卷 期：2015年第5卷第6期
页 面：277-285页
学科分类：0710[理学-生物学] 1008[医学-中药学(可授医学、理学学位)] 0832[工学-食品科学与工程（可授工学、农学学位）] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0703[理学-化学] 0901[农学-作物学] 0902[农学-园艺学] 100602[医学-中西医结合临床] 10[医学]
基 金：The authors gratefully acknowledge Fundacāo para a Ciência e a Tecnologia for financial support of the projects PTDC/QUI/67165/2006 and PEst-OE/QUI/UI0612/2013 The authors also thank the European Commission for approval of the INOVAFUNAGEING commitment and the support of the project“PERsonalised ICT supported Service for Independent Living and Active Ageing”,FP7-ICT-2013-10,Project Nr.610359,2013-2016 The collaboration of the staff from the Herbarium of Jardim Botanico da Madeira,for providing plant material,is also acknowledged
主 题：a-Glucosidase inhibition In vitro digestion HPLC-DAD-ESI-MS/MS Antioxidant activity Flavonoid glycosides
摘 要：The inhibition of a-glucosidase and glucose-6-phosphatase,two enzymes involved in the carbohydrate metabolism,is an important target to control glycaemia on individuals with type 2 *** this work we report for the first time the inhibition of both enzymes by the antihyperglycemic n-butanol extract from Genista tenera(Fabaceae).This extract decreased a-glucosidase and glucose-6-phosphatase activities to 0.97 and 80.25%,respectively,being more effective than acarbose,and phlorizin,the positive controls,which reduced enzymes activities only to 17.39 and 96.06%.Once inflammation and oxidative stress are related to diabetic impairments,the anti-inflammatory activity of the extract was also evaluated,through its inhibitory activity over COX-1 enzyme(47.5%inhibition).Moreover,after induction of oxidative stress by UV radiation,the viability of irradiated rat liver hepatoma cells exposed to the extract was significantly higher(67.82%)than that promoted by ascorbic acid,the positive control(45.05%).In addition,the stability of the extract under gastrointestinal conditions was evaluated by HPLC–DAD-ESI–MS/*** diglycosides were identified as the main constituents of the extract,and no alterations in the chemical composition nor in the antioxidant activity were observed after in vitro digestion with artificial gastric and pancreatic juices.
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