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Effect of Thermal Processing, Press Throughput and Roller-Die Gap on Physicochemical Properties of Broiler Feed Pellets

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Journal of Agricultural Science and Technology(A) 2016年第2期6卷 98-107页

作者： Keysuke Muramatsu isabel Cristina Mores Vaccari Cibele silva Minafra Rafael Femando Sens FabianoDahlke Alex Maiorka DSM Nutritional Products Av. Eng. Billings 1729 Sao Paulo 05321-010 Brazil Instituto Federal Goiano Rio Verde Goias 75901-970 Brazil BRF-Rodovia BR 277 3001 Mossungue Curitiba 82305-100 Brazil Universidade Federal de Santa Catarina (UFSC) Rod. Admar Gonzaga 1346 Florianopolis 88034-000 Brazil Universidade Federal do Parana Rua dos Funcionarios 1540 Curitiba 80035-050 Brazil

The present study aimed to evaluate the effect of thermal processing, press throughput and roller-die gap on pellet physical quality, protein solubility and starch gelatinization in a corn-soybean based broiler diet. The different processing factors were combined in a 2 × 5 × 3 factorial arrangement with eight randomized block consisting of eight production series： two thermal processing treatments （conditioning-pelleting or conditioning-expanding-pelleting）, five press throughput levels （18, 21, 24, 27 and 30 ton/h） and three roller-die gap （0.50, 0.75 and 1.00 mm）, which resulted in 30 different processed feeds. For determination of pellet durability index （PDI）, amount of intact pellets, degree of starch gelatinization and protein solubility, one composed feed sample was collected per treatment in each of eight production series, totaling eight replicates per treatment. Data concerning the amount of intact pellet and protein solubility in KOH were transformed using a variation of Box-Cox transformation in order to fit a normal distribution （P 〉 0.05）. Pellet physical quality was enhanced by conditioning-expanding treatment （P 〈 0.01）, narrower roller-die-gap （P 〈 0.01） and reduced press throughput （P 〈 0.01）. Protein solubility in KOH was impaired （P 〈 0.01） in the expander treatment and larger roller-die distance （0.75 mm and 1.00 mm）（P 〈 0.01）. Finally, greater starch gelatinization degree was achieved in expanded treatment （P 〈 0.01） and in 1.00 mm roller-die gap （P 〈 0.05）. The factors evaluated in this study resulted in interactions and significant effects on physicochemical properties of broiler feed pellets.

关键词： Conditioning expanding press throughput roller-die gap pellet.

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Oral microbiome and risk of malignant esophageal lesions in a high-risk area of China:A nested case-control study

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Chinese Journal of Cancer Research 2020年第6期32卷 742-754页

作者： Fangfang Liu Mengfei Liu Ying Liu Chuanhai Guo Yunlai Zhou Fenglei Li Ruiping Xu Zhen Liu Qiuju Deng Xiang Li Chaoting Zhang Yaqi Pan Tao Ning Xiao Dong Zhe Hu Huanyu Bao Hong Cai isabel Dos Santos silva Zhonghu He Yang Ke Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Laboratory of GeneticsPeking University Cancer Hospital&InstituteBeijing 100142China Novogene Co. LtdBeijing 100080China Hua County People's Hospital Anyang 456400China Anyang Cancer Hospital Anyang 455000China Department of Non-communicable Disease Epidemiology London School of Hygiene&Tropical MedicineLondon WC1E 7HTUK

Objective:We aimed to prospectively evaluate the association of oral microbiome with malignant esophageal lesions and its predictive potential as a biomarker of ***:We conducted a case-control study nested within a population-based cohort with up to 8 visits of oral swab collection for each subject over an 11-year period in a high-risk area for esophageal cancer in *** oral microbiome was evaluated with 16 S ribosomal RNA(rRNA)gene sequencing in 428 pre-diagnostic oral specimens from 84 cases with esophageal lesions of severe squamous dysplasia and above(SDA)and 168 matched healthy *** analysis was performed to identify taxa of differential *** oral species together with subject characteristics were evaluated for their potential in predicting SDA risk by constructing conditional logistic regression ***:A total of 125 taxa including 37 named species showed significantly different abundance between SDA cases and controls(all P***:The oral microbiome may play an etiological and predictive role in esophageal cancer,and it holds promise as a non-invasive early warning biomarker for risk stratification for esophageal cancer screening programs.

关键词： Early warning biomarker esophageal squamous cell carcinoma oral microbiome risk prediction

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Characterization of goat whey proteins and their bioactivity and toxicity assay

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Food Bioscience 2022年第2期46卷 833-843页

作者： Maria isabel Ferreira Campos Paula Perazzo de Souza Barbosa Laura Junqueira Camargo Luciano Da silva Pinto Bianca Mataribu Catarina Serr˜ao Luis Fernando Marques-Santos Jos´e Hon´orio Lopes Julia Mariano Caju de Oliveira Carlos Alberto de Almeida Gadelha Tatiane Santi-Gadelha Programa de P´os-Graduaç˜ao em Ciˆencia e Tecnologia de Alimentos Universidade Federal da ParaíbaJo˜ao PessoaParaíbaBrazil Departamento de Biologia Molecular Universidade Federal da ParaíbaJo˜ao PessoaParaíbaBrazil Centro de Desenvolvimento Tecnol´ogico Universidade Federal de PelotasRio Grande do SulBrazil Programa de P´os-Graduaç˜ao em Ciˆencias da Nutriç˜ao Universidade Federal da ParaíbaJo˜ao PessoaParaíbaBrazil

Goat whey is an industry-discarded dairy by-product that has interesting nutritional value and a nutrient composition with important functional potential,where proteins have a high biological value and play an important role in the organism’s ***,the aim of this study was to identify,quantify,concentrate and investigate the capacity of goat whey proteins to perform different biological activities and to evaluate their acute *** proteins were quantified and identified by Bradford,Kjehdal and SDS-PAGE *** activity was performed against Listeria monocytogenes,Salmonella spp.,Staphylococcus aureus,Pseudomonas aeruginosa and Escherichia coli microorganisms and showed MIC ranging from 15 to 120μg mL-1,with the highest inhibition for *** *** activity was performed against human malignant melanoma cells and their proliferation inhibition was up to 66.8%within 72 h of *** highest antioxidant capacity was obtained at concentration 2 mg that showed 29.69%of inhibition of DPPH radical *** acute toxicity test was performed using Artemia franciscana larvae in which whey proteins were able to preserve larval survival throughout ***,we can conclude that goat’s whey has proteins that can play a positive effect on health,with antimicrobial activity and without *** present study highlights the great potential of goat’s whey to be developed as a safe biopreservative or as a functional ingredient in the food industry.

关键词： Goat whey proteins Malignant cells Antimicrobial Antioxidant capacity Whey protein functionality

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CDX2 Overexpression in Barrett’s Esophagus and Esophageal Adenocarcinoma

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Journal of Cancer Therapy 2014年第7期5卷 657-663页

作者： Leandro Almeida Streher Vinicius Campos Guilherme da silva Mazzini Marcelo Binato Luise Meurer Maria isabel Edelweiss Richard Ricachenevsky Gurski Gastrointestinal Surgery Department Hospital de Clínicas de Porto Alegre Porto Alegre Brazil School of Medicine Universidade Federal do Rio Grande do Sul Porto Alegre Brazil

Background: Patients with Barrett’s esophagus have an increased risk of developing esophageal adenocarcinoma. Our purpose was to determine CDX2 expression in esophageal mucosa and establish a correlation between this marker and the progression of disease. Methods: We analyzed biopsies and surgical specimens from 150 patients who were divided into five groups according to histopathological diagnosis: G1, normal mucosa (n = 29);G2, esophagitis (n = 19);G3, columnar epithelium without intestinal metaplasia (n = 26);G4, Barrett’s esophagus (n = 32), and G5, adenocarcinoma (n = 44). Immuno-histochemical determination of CDX2 expression was considered positive in the presence of nuclear staining. Results: No CDX2 expression was detected in the G1 or G3 groups;5% of G2, 62.5% of G4 and 70.5% of G5 patients were CDX2 positive. There was a statistically significant difference between the G4 and G5 groups compared to the G1, G2 and G3 (p < 0.05). Conclusions: CDX2 expression was observed among patients with Barrett’s esophagus and adenocarcinoma compared to other groups. CDX2 was not expressed in the phases preceding Barrett’s esophagus, but there was no linear correlation between CDX2 expression and metaplasia-adenocarcinoma progression.

关键词： CDX2 Barrett’s Esophagus Intestinal Metaplasia Esophageal Adenocarcinoma Immunohistochemistry

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Ingredients from integral valorization of isabel grape to formulate goat yogurt with stimulatory effects on probiotics and beneficial impacts on human colonic microbiota in vitro

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Food Science and Human Wellness 2023年第4期12卷 1331-1342页

作者： Francyeli Araújo silva Rita de Cássia Ramos do Egypto Queiroga Evandro Leite de Souza Glenise Bierhalz Voss Maria Manuela Estevez Pintado Margarida Angélica da silva Vasconcelos Department of Nutrition Federal University of PernambucoRecife 50670-420Brazil Department of Nutrition Health Sciences CenterFederal University of ParaíbaJoão Pessoa 58050-585Brazil Universidade Católica Portuguesa CBQF-Centro de Biotecnologia e Química Fina-Laboratório AssociadoEscola Superior de BiotecnologiaPorto 4169-005Portugal

isabel grape(IG)products have high contents of phenolic compounds and fiber recognized for their positive impacts on microorganisms associated with health benefits to *** study evaluated the effects of goat yogurts formulated with ingredients from IG integral valorization on the growth and metabolism of different probiotic strains,as well as on the population of selected bacterial groups and metabolic activity of human colonic microbiota in *** yogurts with IG ingredients(IGI)stimulated the growth of tested Lactobacillus and Bifidobacterium probiotic strains during a 48-h cultivation,as well as decreased the pH values and enhanced the organic acid *** yogurts with IGI increased the population of Lactobacillus *** Bifidobacterium *** a 24-h in vitro colonic fermentation.A stable Firmicutes:Bacteroidetes ratio close to 1 was found in media with goat yogurt formulations during the colonic fermentation,being similar to the effect caused by *** yogurt formulations with IGI caused increased production of short-chain fatty acids and sugar consumption during colonic *** yogurts with IGI should be a valuable strategy for development of novel added-value foods with beneficial effects on gut microbiota and human health.

关键词： Goat dairy product Grape Beneficial microorganisms Gut microbiota Modulatory effects

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Methane production and small intestinal bacterial overgrowth in children living in a slum

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World Journal of Gastroenterology 2012年第41期18卷 5932-5939页

作者： Carolina Santos Mello Soraia Tahan Lígia Cristina FL Melli Mirian silva do Carmo Rodrigues Ricardo Martin Pereira de Mello isabel Cristina Affonso Scaletsky Mauro Batista de Morais Pediatric Gastroenterology Division Federal University of Sao Paulo04039-031 Sao PauloBrazil Center Foundation Institute of Education for Osasco 06020-190 Sao PauloBrazil Infectology Division Federal University of Sao Paulo04025-010 Sao PauloBrazil Microbiology Immunology and Parasitology DivisionFederal University of Sao Paulo 04023-062 Sao PauloBrazil

AIM:To analyze small intestinal bacterial overgrowth in school-aged children and the relationship between hydrogen and methane production in breath ***:This transversal study included 85 children residing in a slum and 43 children from a private school,all aged between 6 and 10 years,in Osasco,*** characterization of the groups,data regarding the socioeconomic status and basic housing sanitary conditions were *** data was obtained in children from both *** children completed the hydrogen(H 2) and methane(CH 4) breath test in order to assess small intestinal bacterial overgrowth(SIBO).SIBO was diagnosed when there was an increase in H 2 ≥ 20 ppm or CH 4 ≥ 10 ppm with regard to the fasting value until 60 min after lactulose ***:Children from the slum group had worse living conditions and lower nutritional indices than children from the private *** was found in 30.9%(26/84) of the children from the slum group and in 2.4%(1/41) from the private school group(P = 0.0007).Greater hydrogen production in the small intestine was observed in children from the slum group when compared to children from the private school(P = 0.007).A higher concentration of hydrogen in the small intestine(P < 0.001) and in the colon(P < 0.001) was observed among the children from the slum group with SIBO when compared to children from the slum group without *** production was observed in 63.1%(53/84) of the children from the slum group and in 19.5%(8/41) of the children from the private school group(P < 0.0001).Methane production was observed in 38/58(65.5%) of the children without SIBO and in 15/26(57.7%) of the children with SIBO from the *** production of hydrogen was lower in methaneproducing children(P = 0.017).CONCLUSION:Children who live in inadequate environmental conditions are at risk of bacterial overgrowth and methane *** is a substrate for methane production in the colon.

关键词： Bacterial overgrowth Breath test Children Colon Hydrogen Methane Small intestine

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Differences in viral kinetics between genotypes 1 and 3 of hepatitis C virus and between cirrhotic and non-cirrhotic patients during antiviral therapy

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World Journal of Gastroenterology 2006年第45期12卷 7271-7277页

作者： José Eymard Medeiros-Filho isabel Maria Vicente Guedes de Carvalho Mello Joo Renato Rebello Pinho Avidan U Neumann Fernanda de Mello Malta Luiz Caetano da silva Flair José Carrilho Hepatology Branch Department of Gastroenterology University of So Paulo School of Medicine So Paulo Brazil Viral Immu-nology Laboratory Instituto Butantan So Paulo Brazil Hepatology Branch Department of Gastroenterology University of S?o Paulo School of Medicine So Paulo Brazil Faculty of Life Sciences Bar-Ilan Univer-sity Ramat-Gan Israel Virology Branch Instituto Adolfo Lutz So Paulo Brazil

AIM： TO evaluate the impact of hepatitis C virus （HCV） infection with genotype 1 or 3 and the presence or absence of liver cirrhosis （LC） in the early viral kinetics response to treatment. METHODS： Naive patients （n = 46） treated with interferon-α （IFN-α） and ribavirin and followed up with frequent early HCV-RNA determinations were analysed. Patients were infected with genotype 1 （n = 28, 7 with LC） or 3 （n = 18, 5 with LC）. RESULTS： The first phase decline was larger in genotype 3 patients than in genotype 1 patients （1.72 vs 0.95 log IU/mL, P 〈 0.001）. The second phase slope decline was also larger in genotype 3 patients than in genotype 1 patients （0.87 vs 0.15 log/wk, P 〈 0.001）. Differences were found in both cirrhotic and non-cirrhotic patients. Genotype 1 cirrhotic patients had a slower 2^nd phase slope than non-cirrhotic patients （0.06 vs 0.18 log/wk, P 〈 0,02）. None of genotype 1 cirrhotic patients had a 1^st phase decline larger than 1 log （non-cirrhotic patients： 55%, P 〈 0.02）. A similar trend toward a slower 2nd phase slope was observed in genotype 3 cirrhotic patients but the 1^st phase slope decline was not different. Sustained viral response was higher in genotype 3 patients than in genotype 1 patients ,（72% vs 14%, P 〈 0.001） and in genotype 1 non-cirrhotic patients than in genotype 1 cirrhotic patients （19% vs 0%）. A second phase decline slower than 0.3 log per week was predictive of non-response in all groups. CONCLUSION： Genotype 3 has faster early viral decline than genotype 1. Cirrhosis correlates with a slower 2nd phase decline and possibly with a lower 1^st phase slope decline in genotype 1 patients.

关键词： Hepatitis C virus Treatment Early kinetics Cirrhosis Genotypes

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Viticultural Performance of Merlot and Cabernet Sauvignon （Vitis vinifera L.） Cultivated in High Altitude Regions of Southern Brazil

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Journal of Life Sciences 2015年第9期9卷 399-410页

作者： Jaqueline Nogueira Muniz Suzeli Simon Alberto Fontanella Brighenti Luciane isabel Malinovski Carolina Pretto Panceri Gabriella Vanderlinde Juliana Welter Debora Dal Zotto Aparecido Lima da silva Santa Catarina Federal University Plant Genetic Resources Post Graduate Program Rod. Admar Gonzaga 1346 ItacorubiFlorian6polis 88034-001 SC Brazil Santa Catarina State Agricultural Research and Rural Extension Agency EPAGRI Rua Josd Arafijo Lima 102 CEP S~oJoaquim 88600-000 SC Brazil Santa Catarina Federal University Food Science Post Graduate Program Rod. Admar Gonzaga 1346 CEP FIorian6polis88034-001 SC Brazil Santa Catarina Federal University Agronomy College Rod. Admar Gonzaga 1346 Itaeorubi Florian6polis 88034-001 SC Brazil

Different studies show that the altitude regions of the State of Santa Catarina have great potential for the production of grapevine varieties. The aim of this study was to characterize the phenological development, determine the thermal requirements in degree-days （DD）, the index heliothermic （IH） and climatic variables during the vegetative and productive development of varieties Merlot and Cabernet Sauvignon grown in Campo Belo do Sul （27°40′04″ S, 50°44′48″ W, altitude 950 m a.s.l.） and Sao Joaquim （28°15′13″ S, 49°57′02″ W, altitude 1,400 m a.s.l.） during 2012/2013 cycle. Phenological stages evaluated were bud break, full bloom, veraison and maturity. Higher temperatures, global radiation, PAR and lower volumes of rainfall were the main climatic parameters that differentiated Campo Belo do Sul from Sao Joaquim. Due to occurrence of higher temperatures, Campo Belo do Sul presented thermal summation around 40% higher than Sao Joaquim. The low temperatures of Sao Joaquim extended the period of grape ripening and were responsible for the highest levels of titratable acidity and the higher concentrations of anthocyanins and total polyphenols in both varieties. The results show that in the altitude ranges studied （900 and 1400 m）, climatic behavior and viticultural performance of Cabernet Sauvignon and Merlot were suitable for producing quality wines.

关键词： Climate phenology technological maturation total polyphenols.

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Differences in age modulates neutrophils function

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Advances in Biological Chemistry 2014年第1期4卷 51-58页

作者： Claudia J. Lagranha Tatiana C. Alba-Loureiro Aline isabel da silva Danuta Duarte Bezerra de Lima Anderson Apolonio Pedroza Diorginis Ferreira Tania Pithon-Curi Department of Nutrition Federal University of Pernambuco Recife Brazil Department of Physical Education and Sports Science Federal University of Pernambuco Vitoria de Santo Ant&atilde o Brazil Department of Physiology and Biophysics Institute of Biomedical Sciences University of S&atilde o Paulo S&atilde o Paulo Brazil Post-Graduate Program in Human Movement Science Biological Sciences and Health Center Cruzeiro do Sul University Sao Paulo Brazil

The sexual maturation in all mammals is the period in which the quiescent gonads are activated by gonadotropins from anterior pituitary, increasing the secretion of sexual hormones. Sexual maturation it is also related with the development of several other body features such as body mass and maturation of the circulatory, skeletal and hematopoietic systems. The aim of the present study was to evaluate the function of neutrophils submitted to in vivo lower and higher concentration of testosterone (sexually immature: 60 days and sexually mature: 90 days). Using different approaches we evaluated cell viability and function and gene expression in rat neutrophils from 60 and 90 days-old animals. Neutrophils from 90 days-old rats showed a decrease in phagocytic and fungicidal capacity, without change in cellular viability. Additionally, we verified that sexual maturation induced increase in production of reactive oxygen and nitrogen species (RONS) and also in TNF-α, IL-6 and IL-10 cytokines. In conclusion, our data suggest that increase in testosterone levels induced significant alteration in neutrophil function, impairing phagocytic capacity.

关键词： Testosterone Neutrophils Function NADPH-Oxidase Components Sexual Maturation

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Assessment of Climate Change over South America under RCP 4.5 and 8.5 Downscaling Scenarios

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American Journal of Climate Change 2014年第5期3卷 512-527页

作者： Sin Chan Chou Andre Lyra Caroline Mourao Claudine Dereczynski isabel Pilotto Jorge Gomes Josiane Bustamante Priscila Tavares Adan silva Daniela Rodrigues Diego Campos Diego Chagas Gustavo Sueiro Gracielle Siqueira Jose Marengo National Institute for Space Research Cachoeira PaulistaBrazil Department of Meteorology Federal University of Rio de JaneiroRio de JaneiroBrazil National Center for Natural Disaster Monitoring and Warning Sao Jose dos CamposBrazil

Four sets of downscaling simulations based on the Eta Regional Climate Model forced by two global climate models, the HadGEM2-ES and the MIROC5, and two RCP scenarios—8.5 and 4.5, have been carried out. The objective of this work is to assess the climate change over South America based on the Eta simulations. The future changes are shown in timeslices of 30 years: 2011-2040;2041-2070 and 2071-2100. The climate change response of the Eta simulations nested in HadGEM2-ES is larger than the Eta nested in MIROC5. Major warming area is located in the central part of Brazil. In austral summer, the reduction of precipitation in the central part and the increase in the southeastern part of the continent are common changes in these simulations, while the EtaHadGEM2-ES intensifies the decrease of precipitation in central Brazil, the Eta-MIROC5 expands the area of increase of precipitation in southern Brazil toward the end of the century. In austral winter, precipitation decrease is found in the northern part of South America and in most of Central America, whereas the reduction in southeastern South America is limited to near coastal region. The time series of temperatures show that warming trends are larger in the Eta-HadGEM2-ES than in the Eta-MIROC5 simulations. Heavier precipitation rates are projected in the Central-South of Brazil toward the end of the century. Increase in the length of consecutive dry days (CDD) in Northeast of Brazil and the decrease of consecutive wet days (CWD) in the Amazon region are common features in these simulations.

关键词： South America Climate Downscaling Climate Change Assessment Climatic Extreme Indicators Eta Model

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