Characterization of goat whey proteins and their bioactivity and toxicity assay

作     者：Maria Isabel Ferreira Campos Paula Perazzo de Souza Barbosa Laura Junqueira Camargo Luciano Da Silva Pinto Bianca Mataribu Catarina Serr˜ao Luis Fernando Marques-Santos Jos´e Hon´orio Lopes Julia Mariano Caju de Oliveira Carlos Alberto de Almeida Gadelha Tatiane Santi-Gadelha 

作者机构：Programa de P´os-Graduaç˜ao em Ciˆencia e Tecnologia de AlimentosUniversidade Federal da ParaíbaJo˜ao PessoaParaíbaBrazil Departamento de Biologia MolecularUniversidade Federal da ParaíbaJo˜ao PessoaParaíbaBrazil Centro de Desenvolvimento Tecnol´ogicoUniversidade Federal de PelotasRio Grande do SulBrazil Programa de P´os-Graduaç˜ao em Ciˆencias da Nutriç˜aoUniversidade Federal da ParaíbaJo˜ao PessoaParaíbaBrazil 

出 版 物：《Food Bioscience》 (食品生物科学（英文）)

年 卷 期：2022年第46卷第2期

页      面：833-843页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：The authors would like to thank CAPES-Higher Education Personnel Improvement Coordination IDEP/UFPB-Research Development Institute and CNPq-Brazilian National Coucil for Scientific and Technological Development for funding this research.To the goat sector of Campus IV of UFPB for giving the goat milk for the experiments.And to UFPel for its support in conducting antiproliferative experiments 

主　　题：Goat whey proteins Malignant cells Antimicrobial Antioxidant capacity Whey protein functionality 

摘      要：Goat whey is an industry-discarded dairy by-product that has interesting nutritional value and a nutrient composition with important functional potential,where proteins have a high biological value and play an important role in the organism’s ***,the aim of this study was to identify,quantify,concentrate and investigate the capacity of goat whey proteins to perform different biological activities and to evaluate their acute *** proteins were quantified and identified by Bradford,Kjehdal and SDS-PAGE *** activity was performed against Listeria monocytogenes,Salmonella spp.,Staphylococcus aureus,Pseudomonas aeruginosa and Escherichia coli microorganisms and showed MIC ranging from 15 to 120μg mL-1,with the highest inhibition for *** *** activity was performed against human malignant melanoma cells and their proliferation inhibition was up to 66.8%within 72 h of *** highest antioxidant capacity was obtained at concentration 2 mg that showed 29.69%of inhibition of DPPH radical *** acute toxicity test was performed using Artemia franciscana larvae in which whey proteins were able to preserve larval survival throughout ***,we can conclude that goat’s whey has proteins that can play a positive effect on health,with antimicrobial activity and without *** present study highlights the great potential of goat’s whey to be developed as a safe biopreservative or as a functional ingredient in the food industry.