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Acta Pharmacologica Sinica 2004年第1期 100-105页

作者： Shao-hua SHI, Shu-sen ZHENG, Chang-ku JIA, You-fa ZHU, Hai-yang XIEDepartment of Hepatobiliary surgery, the First Affiliated Hospital, Medical College, zhejiang university Department of Pathology, Medical College, zhejiang university institute of surgery, zhejiang university, hangzhou 310003, china

AIM: To investigate the inhibitory effect of tea polyphenols (TP)on the transforming growth factor-pi (TGF-β1) expression in rat model of cyclosporine A (CsA)-induced chronic nephrotoxicity. METHODS: The rat model of CsA-induced chronic nephrotoxicity was used, 4 groups of rats were respectively treated with vehicle (0.1 mL·lg-1-d-1 sc), TP (80 mg·kg-1·d-1 ig), CsA (15 mg·2kg-1·d-1sc) and TP plus CsA (CsA 15 mg·kg-1·d-1 sc+TP 80 mg·kg-1·d-1 ig). At the end of day 28 of treatment, serum and urine are analyzed for creatinine clearance, kidney tissue for pathologic analysis. The TGF-β1 mRNA and its protein expression were detected by RT-PCR, immunohistochemistry, and Western blot. RESULTS: CsA-treated rats had increased renal expression of TGF-pl mRNA and its protein, compared with the vehicle- or TP- treated

关键词： tea cyclosporine A transforming growth factor-β kidney polymerase chain reaction immunohistochemistry Western blotting

Top 10 Influential Events in carbon neutrality and climate change response for 2023

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Frontiers in Energy 2024年第1期18卷 1-3页

作者： Research institute of Carbon Neutrality of Shanghai Jiao Tong university Research institute of Carbon Neutrality Shanghai Jiao Tong UniversityShanghai 200240China

In 2023, the global community intensified its efforts towards achieving carbon neutrality and addressing climate change, witnessing several impactful events. Research institute of Carbon Neutrality, Shanghai Jiao Tong university compiled the Top 10 Events (in chronological order) in the field of global carbon neutrality and climate change response for 2023, to share with readers these significant moments that document the ongoing ambition and collective efforts of human society.

关键词： neutral collective intensified

中国政治经济学年度发展报告（2023）（下）

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政治经济学评论 2024年第3期 90+207-208页

作者： Center for Studies of Contemporary Political Economy,Research institute for 21st-Century Marxism,Nankai university·university of Chinese Academy of Social Science Center for Teaching and Research on Capital,Renmin university of china National institute of Socialist Political Economy with Chinese Characteristics,Renmin university of china 南开大学·中国社会科学院大学21世纪马克思主义研究院当代政治经济学研究中心中国人民大学《资本论》教学与研究中心中国人民大学全国中国特色社会主义政治经济学研究中心

编者按2023年，高质量发展扎实推进，新质生产力加快形成，我国经济总体回升向好，圆满实现发展主要预期目标。为针对性地破解经济恢复发展中的难题，聚焦推进中国式现代化进程中的重点、难点问题，中国政治经济学界开展系统深入研究，...

编者按2023年，高质量发展扎实推进，新质生产力加快形成，我国经济总体回升向好，圆满实现发展主要预期目标。为针对性地破解经济恢复发展中的难题，聚焦推进中国式现代化进程中的重点、难点问题，中国政治经济学界开展系统深入研究，取得系列代表性成果。为准确刻画学界研究进展和轨迹，推动中国经济学理论体系的构建和完善，本刊精选议题如期奉上《中国政治经济学年度发展报告（2023）》。自2010年起，本刊连续十四年发布《中国政治经济学年度发展报告》，“年度发展报告”专栏已成为本刊标志性特色栏目。2023年报告聚焦12个专题，分为（上）（下）两篇，其中专题1—6于报告（上）篇刊登，专题7—12由报告（下）篇刊登。2023年报告（下）选题如下：

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Interaction between a Nanovirus-like Component and the Tobacco Curly Shoot Virus/Satellite Complex

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Acta Biochimica et Biophysica Sinica 2005年第1期37卷 25-31页

作者： Pei-Jun WU Xue-Ping ZHOU~* institute of Biotechnology zhejiang university,hangzhou 310029,china institute of Biotechnology Zhejiang UniversityHangzhou 310029 China

The biological role of DNA1,a nanovirus-like component shown to be associated with thebegomovirus/satellite complex,has not yet been ***,we demonstrated that DNAl of Tobaccocurly shoot virus isolate Y35(TbCSV-Y35)attenuated leaf-curling symptoms induced by TbCSV-Y35 orTbCSV-Y35 plus Y35 DNAβ in the early stage of symptom development and induced leaf cluster at a laterstage of symptom development in Nicotiana benthamiana *** leaf disc assay demonstrated thatTbCSV-Y35 DNA 1 replicated *** blot analysis revealed that TbCSV-Y35 DNA 1 re-duced viral DNA *** DNA accumulation was not reduced when plants were co-inoculatedwith TbCSV-Y35 DNAβ,but the TbCSV-Y35 DNAβ level was dramatically reduced in the presence ofTbCSV-Y35 DNA *** determine whether the interaction between TbCSV/satellite complex and DNA 1 hadisolate specificity,DNAI of TbCSV isolate Y132 was cloned and *** was found to have 75%nucleotide sequence identity with TbCSV-Y35 DNA *** tests showed that TbCSV-Y 132 DNA 1 hadno effect on the symptoms induced by TbCSV-Y35 or TbCSV-Y35 and Y35 DNAβ in ***,although Y 132 DNA1 could replicate in these plants.

关键词： Tobacco curly shoot virus DNA1 DNAβ symptom replication interaction

Identification of a bi-directional promoter from Tomato yellow leaf curl china virus

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Progress in Natural Science:Materials International 2006年第3期16卷 281-286页

作者： GUAN Cuiping and ZHOU Xueping (institute of Biotechnology, zhejiang university, hangzhou 310029, china) institute of Biotechnology Zhejiang University Hangzhou 310029 China

A bi-directional promoter of Tomato yellow leaf curl china virus (TYLCCNV) was obtained with the total DNA from TYLCCNV isolate Y10 infected tobacco leaves as a template. Plant expression vectors were constructed by fusing the amplified DNA fragment with the gus gene and nopaline terminator in different orientations. The vectors containing promoter fragments were transferred into leaf cells and plant stems of Nicotiana benthamiana by Agrobacterium-mediated method. Transient expression results showed that both the complementary and virion-sense promoters could drive the gus gene to express, and the GUS activity of the complementary-sense promoter was stronger than that of the virion-sense. Co-expression of the vector containing βC1 gene of TYLCCNV DNAβ with the vector containing a bi-directional promoter revealed that the βC1 protein has no impact on expression of either the virion- or the complementary-sense promoter.

关键词： Tomato yellow leaf curl china virus bi-directional promoter transient expression geminivirus.

Multiple products of B-splines used in CAD system

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Progress in Natural Science:Materials International 2006年第4期16卷 425-430页

作者： XU Huixia and WANG Guojin institute of Images and Graphics, zhejiang university, hangzhou 310027, china State Key Laboratory of CAD&CG, zhejiang university, hangzhou 310027, china institute of Images and Graphics Zhejiang University Hangzhou 310027 China State Key Laboratory of CAD and CG Zhejiang University Hangzhou 310027 China

The function upgrade of computer aided design (CAD) system requested that the multiple product of B-spline functions should be represented as a linear combination of some suitable (usually higher-degree) B-splines. In this paper, we apply the theory of spline space and discrete B-splines to deduce the representation of the coefficients of all terms of the linear combination, which can be directly applied to software coding in system development.

关键词： computer aided design (CAD) NURBS Marsden’s identity.

POLYMERIZATION OF γ-STEARYL-α,l-GLUTAMATE N-CARBOXYANHYDRIDE USING RARE EARTH COORDINATION CATALYSTS

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Chinese Journal of Polymer Science 2000年第4期18卷 369-372页

作者： Xiao-ping Hu Jian Wu Zhi-kang Xu Lin-xian Feng institute of Polymer Science, zhejiang university, hangzhou 310027, china Department of Chemistry, zhejiang university, hangzhou 310027, china 浙江大学浙江杭州

A rare earth coordination system was first investigated as a new type of catalyst for the ring-openingpolymerization of α-amino acid N-carboxyanhydrides (NCAs). The results for the polymerization of γ-stearyl-α,L-glutamate(SLG) NCA using neodymium acetylacetonate (Nd(acac)_3)- or neodymium tris(2-ethylhexylphosphonate) (Nd(P_(204))_3)-triethylaluminum-water as catalysts were compared with those using conventional catalysts. It was found that the helicalpoly(γ-stearyl-L-glutamate) with high molecular weight as well as narrow molecular weight distribution can be obtained inthe presence of Nd(acac)_3/AlEt_3-1/2H_2O. The polymer obtained was characterized by IR and NMR spectroscopy.

关键词： Rare earth coordination catalyst Ring-opening polymerization Polypeptide Poly(γ-stearyl-α,L-glutamate)

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Animal experiment and clinical study of effect of gamma-interferon on hepatic fibrosis

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World Journal of Gastroenterology 2001年第1期7卷 42-48页

作者： Hong Lei Weng Wei Min Cai Rong Hua Liu institute of Infectious Diseases, First Affiliated Hospital. Medical School. zhejiang university, hangzhou 310003, zhejiang Province. china institute of Infectious Diseases First Affiliated Hospital Medical School Zhejiang University 261 Qingchun Lu Hangzhou 310003 Zhejiang Province China. syliao@***

AIM: To evaluate the antifibrotic effect of different doses of recombinant human Gamma-Interferon (IFN-gamma) in two rat models of hepatic fibrosis, and to observe its effect on moderate chronic hepatitis B virus fibrosis. METHODS: Hepatic fibrosis was successfully induced in 150 and 196 rats by subcutaneous injection of carbon tetrachloride (CCl4) and intraperitoneal injection of dimethylnitrosamine (DMN), respectively. Each of the two model groups was divided into: (1) fibrotic model group; (2) colchicine treatment group (0.1 mg/kg/day, gastrogavage for 8 weeks); (3) high-dose IFN-gamma group (15 MU/kg per day, i.m. for 8 weeks); (4) medium-dose IFN-gamma group (5 MU/kg daily, i.m. for 8 weeks); and (5) Y low-dose IFN-gamma group (1.67 MU/kg daily, i.m. for 8 weeks). Another group of 10 rats without any treatment was used as normal controls. At the end of the experiment, semi-quantitative histopathological scores of inflammation and fibrosis, liver alpha smooth muscle actin (alpha-SMA) expression level, liver hydroxyl proline content and serum hyaluronic acid levels were compared. And 47 medium chronic hepatitis B viral fibrosis patients were studied. They were given IFN-gamma treatment, 100 MU/day i.m. for the first three months and 100 MU qod i.m. for the next six months. Semi-quantitative pathological scores of inflammation and fibrosis and serum hepatic fibrosis indices were compared within the 9 months. RESULTS: In animal experiment, the pathological fibrosis scores and liver hydroxyl proline content were found to be significantly lower in rats treated with different doses of IFN-gamma as compared with rats in fibrotic model group induced by either CCl4 or DMN, in a dose-dependent manner. For CCl4-induced model, pathological fibrosis scores in high, medium and low doses IFN-gamma groups were 5.10 +/- 2.88, 7.70 +/- 3.53 and 8.00 +/- 3.30, respectively, but the score was 14.60 +/- 7.82 in fibrotic model group. Hydroxyl proline contents were 2.83 +/- 1.18, 3.59

关键词： Animals Antineoplastic Agents dosage Biopsy Carbon Tetrachloride Dimethylnitrosamine Disease Models, Animal Female Hepatitis B, Chronic Humans Hyaluronic Acid Hydroxyproline Interferon-gamma, Recombinant Liver Liver Cirrhosis Liver Function Tests Male Rats Rats, Sprague-Dawley

Highly Sensitive Fluorescent-labeled Probes and Glass Slide Hybridization for the Detection of Plant RNA Viruses and a Viroid

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Acta Biochimica et Biophysica Sinica 2007年第5期39卷 326-334页

作者： Zhiyou DU~(1,2), Bo JIN~3, Wenhong LIU~4, Liang CHEN~1, and Jishuang CHEN~(2,1*) 1 College of Life Sciences, zhejiang university, hangzhou 310029, china 2 institute of Bioengineering, zhejiang Sci-Tech university, hangzhou 310018, china 3 College of Life Sciences, zhejiang Chinese Medical university, hangzhou 310053, china 4 College of Bioengineering, zhejiang Chinese Medical university, hangzhou 310053, china College of Life Sciences Zhejiang UniversityHangzhou 310029 China institute of Bioengineering Zhejiang Sci-Tech UniversityHangzhou 310018 China College of Life Sciences Zhejiang Chinese Medical UniversityHangzhou 310053 China College of Bioengineering Zhejiang Chinese Medical UniversityHangzhou 310053 China

In this study, a modified method of the conventional RNA dot-blot hybridization was established, by replacing32p labels with CY5 labels and replacing nylon membranes with positive-charged glass slides, for detecting plant RNA viruses and a viroid. The modified RNA dot-blot hybridization method was named glass slide hybridization. The optimum efficiency of RNA binding onto the surfaces of activated glass slide was achieved using aminosilane-coated glass slide as a solid matrix and 5×saline sodium citrate （SSC） as a spotting solution. Using a CY5-labeled DNA probe prepared through PCR amplification, the optimized glass slide hybridization could detect as little as 1.71 pg of tobacco mosaic virus （TMV） RNA. The sensitivity of the modified method was four times that of dot-blot hybridization on nylon membrane with a32P-labeled probe. The absence of false positive within the genus Potyvirus [potato virus A, potato virus Y （PVY） and zucchini yellow mosaic virus] showed that this method was highly specific. Furthermore, potato spindle tuber viroid （PSTVd） was also detected specifically. A test of 40 field potato samples showed that this method was equivalent to the conventional dot-blot hybridization for detecting PVY and PSTVd. To our knowledge, this is the first report of using dot-blot hybridization on glass slides with fluorescent-labeled probes for detecting plant RNA viruses and a viroid.

关键词： glass slide hybridization fluorescent labeling dot-blot hybridization virus viroid

Two-dimensional electrophoresis analysis of proteomics based on image feature and mathematical morphology

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Science china Chemistry 2005年第4期48卷 361-367页

作者： SHEN Peng1,FAN Xiaohui2,ZENG Zhen2 & CHENG Yiyu2 1.The First Affiliated Hospital,College of Medicine,zhejiang university,hangzhou 310003,china 2.Pharmaceutical Informatics institute,zhejiang university,hangzhou 310027,china The First Affiliated Hospital College of Medicine Zhejiang University Hangzhou China Pharmaceutical Informatics institute Zhejiang University Hangzhou China

In this paper,a novel method to automatically detect protein spots on a two-dimensional (2-D) electrophoresis gel image is proposed to implement proteomics analysis of complex *** the basis of the identifying spots results based on color variation and spot size features,morphological feature is introduced as a new criterion to distinguish protein spots from non-protein ***-sharpening,edge-detecting and morphological feature extraction methods were consequently combined to detect protein spots on a 2-D electrophoresis gel image subject to strong *** proposed method was applied to detect the protein spots of proteomic gel images from *** cell,human kidney tissue and human *** results demonstrated that this method is more accurate and reliable than previous methods such as PDQuest 7.2 and ImageMaster 5.0 software for detecting protein spots on gel images with strong interferences.

关键词： proteomics, 2-dimensional electrophoresis, protein spot recognition, analytical chemistry for life, morphological feature, image recognition.

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