Identification of a bi-directional promoter from Tomato yellow leaf curl China virus

作     者：GUAN Cuiping and ZHOU Xueping (Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China) 

作者机构：[a]Institute of Biotechnology Zhejiang University Hangzhou China 

出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))

年 卷 期：2006年第16卷第3期

页      面：281-286页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：Supported by National Fund for Distinguished Young Scholars (Grant No. 30125032) National Natural Science Foundation of China (Grant No.30530520) 

主　　题：Tomato yellow leaf curl China virus bi-directional promoter transient expression geminivirus. 

摘      要：A bi-directional promoter of Tomato yellow leaf curl China virus (TYLCCNV) was obtained with the total DNA from TYLCCNV isolate Y10 infected tobacco leaves as a template. Plant expression vectors were constructed by fusing the amplified DNA fragment with the gus gene and nopaline terminator in different orientations. The vectors containing promoter fragments were transferred into leaf cells and plant stems of Nicotiana benthamiana by Agrobacterium-mediated method. Transient expression results showed that both the complementary and virion-sense promoters could drive the gus gene to express, and the GUS activity of the complementary-sense promoter was stronger than that of the virion-sense. Co-expression of the vector containing βC1 gene of TYLCCNV DNAβ with the vector containing a bi-directional promoter revealed that the βC1 protein has no impact on expression of either the virion- or the complementary-sense promoter.