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Cloning and characterization of a mouse liver-specific gene mfrep-1, up-regulated in liver regeneration

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Cell Research 2002年第Z2期 353-361页

作者： JUN VAN, HAD YING, FBI GU, JIN HE, Yu Li LI, Hm MIN LIU, YONG HUA XU Laboratory of Molecular and Cellular Oncology, institute of Biochemistry and Cell Biology, Shanghai institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China Department of Surgery, Huashan Hospital, Fudan University, Shanghai 200031, China Department of pathology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China

Human fibrinogen-related protein-1/liver fibrinogen-related protein-1 (HFREP-l/LFIRE-1), a liver-specific protein, is a member of fibrinogen superfamily that exerts various biological activities. However, the function of HFREP-l/LFIRE-1 in liver remains unknown. Here we isolated its mouse ortholog gene-mouse fibrinogen-related protein-1 (mfrep-1), which encoded 314 amino acids, exhibiting 80.4% similarity to HFREP-l/LFIRE-1. Northern blot analysis revealed that 1.2-kb mfrep-1 mRNA was detected selectively in mouse liver. To explore the function of MFREP-1, we examined the levels of mfrep-1 mRNA during regeneration after 70% partial hepatectomy (PHx) in mice, mfrep-1 mRNA increased in the regenerating liver and reached the first shoulder peak at 2-4 h after PHx. Cycloheximide pretreatment could suppress the induction of mfrep-1, indicating the up-regulation of this gene need de novo protein synthesis. Its mRNA continued to elevate at 6 h thereafter and reached the second peak at 24 h. The enhanced express

关键词： mfrep-1 in silico cloning liver regeneration liver-specific expression.

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LETHAL GRANULOMA

中华医学杂志(英文版) 1974年第4期87卷 67-67页

作者： Department of pathology and Radiology Cancer institute Chinese Academy of Medical Sciences Peking

THE APPLICATION OF BIOLOGICAL STEREOLOGY AND IMAGE ANALYSIS ON SYNAPTIC RESEARCH

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THE APPLICATION OF BIOLOGICAL STEREOLOGY AND IMAGE ANALYSIS ...

Activation of extracellular signal-regulated kinase by TGF-β1 via TβR Ⅱ and Smad 7 dependent mechanisms in human bronchial epithelial BEP2D cells

第十三届国际体视学大会

作者： Xinping Xu Hongyan Zuo Lifeng Wang Yang Li Li Zhao Shaoxia Wang Chengfeng Sun Xiaomin Wang Yabing Gao Ruiyun Peng Dewen Wang Department of Experimental pathology Beijing institute of Radiation Medicine Beijing 100850 China

＜正＞Objective:To investigate the biomedical value of the combination of stereology and image analysis by studying synapse index quantitatively. Method:The method of pathological image analysis can provide quantitative and digital evidence for pathologic *** has now become a necessary auxiliary means for scientific personnel to make an objective,accurate and reliable pathologic *** is an unbiased mathematical technique,which can accurately calculate the length,surface area,volume *** specific objects or *** on previous research of synaptic plasticity for many years,we have got large numbers of synaptic data such as length of active region,width of cleft,thickness of PSD and proteins related to synaptic plasticity by pathological image analysis on ultrastructure *** data were analyzed by the unbiased stereological technique in this *** the expression of protein is one of the methods to study changes of synaptic plasticity. Result:Deviations were sometimes evitable in pathological image *** the unbiased stereological analysis,we speculated synaptic capacity,neuronal density and glial cells density *** index such as area density and number density can measure the two-dimensional structure and three-dimensional space of slices accurately. Conclusion:The combination of pathological image analysis and stereology make us obtain reliable quantitative *** this method structure of tissues and cells can be analyzed more objectively and accurately on three-dimensional level.

关键词： biological stereology pathological image analysis synapse two-dimensional structure three-dimensional space

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Activation of extracellular signal-regulated kinase by TGF-...

Is inconsistency of α-fetoprotein level a good prognosticator for hepatocellular carcinoma recurrence?

中国毒理学会第四届全国学术会议

作者： Yanying Huo, Yingchun Hu, Gang Li, Xinrong He, Pingkun Zhou, Dechang Wu ( Department of Radiation Toxicology and Oncology, Beijing institute of Radiation Medicine Beijing, 100850 Department of pathology, Chongqing University of Medical Science, Chongqing, 400016, PR China)

＜正＞Objective Transforming growth factor-β1 （TGF-β1） can activate mitogen-activated protein kinases （MAPKs） in many types of cells. The mechanism of this activation is not well elucidated. Here, we explored the role of TGF-β/Smads signaling compounds in TGF-β1-mediated activation of extracellular signal-regulated kinase （ ERK） MAPK in

关键词： TGF-β1 TβRⅡ Smad7 Smad 4 ERK

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World Journal of Gastroenterology 2010年第24期16卷 3049-3055页

作者： Chung-Bao Hsieh, Teng-Wei Chen, Division of General Sur-gery, Tri-Service General Hospital, Taipei 114, Taiwan, China Chung-Bao Hsieh, Kuo-Piao Chung, Graduate institute of Health Care Organization Administration, National Taiwan University, Taipei 100, Taiwan, China Chi-Ming Chu, Section of Biostatistics and Informatics, De-partment of Epidemiology, School of Public Health, National Defense Medical Center, Taipei 114, Taiwan, ChinaHeng-Cheng Chu, Department of Internal Medicine, Tri-Service General Hospital, Taipei 114, Taiwan, ChinaCheng-Ping Yu, Department of pathology, Tri-Service General Hospital, Taipei 114, Taiwan, China Division of General Surgery Tri-Service General Hospital Taipei 114 Taiwan China.

AIM: To identify the clinical outcomes of hepato-cellular carcinoma (HCC) patients with inconsistent α-fetoprotein (AFP) levels which were initially high and then low at ***: We retrospectively included 178 patients who underwent liver resection with high preoperative AFP levels (≥ 200 ng/dL). Sixty-nine HCC patients had recurrence during follow-up and were grouped by their AFP levels at recurrence: group Ⅰ, AFP ≤ 20 ng/dL (n = 16); group Ⅱ, AFP 20-200 ng/dL (n = 24); and group Ⅲ, AFP ≥ 200 ng/dL (n = 29). Their preoperative clinical characteristics, accumulated recurrence rate, and recurrence-to-death survival rate were compared. Three patients, one in each group, underwentliver resection twice for primary and recurrent HCC. AFP immunohistochemistry of primary and recurrent HCC specimens were ***: In this study, 23% of patients demon-strated normal AFP levels at HCC recurrence. The AFP levels in these patients were initially high. There were no significant differences in clinical characteristics between the three groups except for the mean recur-rence interval (21.8 ± 14.6, 12.3 ± 7.7, 8.3 ± 6.6 mo, respectively, P 5 cm, total bilirubin > 1.2 mg/dL, vessel invasion, Child classification B, group Ⅲ, and recurrence interval < 12 mo, were risk factors for survival rate. Cox regression analysis was performed and vessel invasion, group Ⅲ, and recurrence interval were independent risk factors. The recurrence inter-val was significant longer in group Ⅰ (P < 0.001). The recurrence-to-death survival rate was significantly bet-ter in group Ⅱ (P = 0.016). AFP staining was strong in the primary HCC specimens and was reduced at recur-rence in group Ⅰ ***: Patients in group Ⅰ with inconsistent AFP levels had a longer recurrence interval and worse recurrence-to-death survival rate than those in group Ⅱ. This clinical presentatio

关键词： Hepatocellular carcinoma Inconsistent α-fetoprotein Outcome Recurrence

应用植源性蜕皮激素调节蚕儿生长发育和增产蚕丝的研究

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昆虫学报 1977年第2期20卷 147-154页

作者： SECTION OF SEEICULTUBAL SERVICE,ACADEMY OF AQRICULTUEAL ANDFORESTEIAL SCIENCES OF CHINALABORATORY OF PHYSIOLOGY AND pathology,KIANGSU institute OF SERICULTURE LABORATORY OF INSECTICIDE AND TOXICOLOGY,PEKING instituteOF ZOOLOGY,ACADEMIA SINICAFIRST EESEARCH LABORATORY,SHANGHAI institute OF ORGANIC CHEMISTRY,ACADEMIA SINICA 中国农林科学院蚕桑科技服务组江苏省蚕业研究所生理病理室中国科学院北京动物研究所药剂毒理室中国科学院上海有机化学研究所第一研究室

本文从生产角度,着重探讨了由筋骨草提取的蜕皮激素(以下简称MH)在调节蚕儿生长发育和增产蚕丝方面的实用价值:(1)家蚕末龄中期(饷食后72小时左右),每头添食MH3微克,可以缩短龄期,提早两天上簇结茧,避免或减少缺叶倒蛋的损失;(2)在见熟... 详细信息

本文从生产角度,着重探讨了由筋骨草提取的蜕皮激素(以下简称MH)在调节蚕儿生长发育和增产蚕丝方面的实用价值:(1)家蚕末龄中期(饷食后72小时左右),每头添食MH3微克,可以缩短龄期,提早两天上簇结茧,避免或减少缺叶倒蛋的损失;(2)在见熟前10小时,每头添食MH2微克,连添二次,可使五龄经过缩短14小时,上蔟开始到上蔟终了的时间,从对照区的39小时缩短到14小时。一般见熟5—10％添食MH2.2微克/头,12小时后即可全部上蔟,熟蚕出现率非常集中,这可大大提高上蔟工效,提高养蚕的劳动生产率:(3)见熟时添食MH还可有效地减少不结茧蚕发生,这不仅在提高JHA增产效果方面,而且在一般养蚕生产中防止不结茧蚕方面,也有重要意义;(4)试验还发现,五龄早期(饷食后24小时)添食适量的MH,有一定的增丝效果,不同试验中,平均茧层量增长幅度4—17％不等;(5)家蚕末龄添食MH,能明显地提高一日茧层量,提高幅度为5—30％,依MH添食时刻为转移。

关键词：蚕儿添食不结茧蚕蜕皮激素甾体激素 MH 生长发育蚕丝动物纤维

A study on pathogenicity of hepatitis G virus

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World Journal of Gastroenterology 2001年第4期7卷 547-550页

作者： Jia-Zhang Xu Zhi-Guo Yang Mei-Zhao Le Mao-Rong Wang Chang-Lun He Yun-Hua Sui institute of Hepatology,the 81st Hospital of PLA,Nanjing 210002,Jiangsu Province,ChinaDepartment of pathology,the 81st Hospital of PLA,Nanjing 210002,Jiangsu Province,China institute of Hepatology the 81st Hospital of PLA Nanjing 210002 Jiangsu Province China.

AIM To study the pathogenicity of hepatitis G virus (HGV) and observe the genesis and pathological process of hepatitis G.METHODS HGV-RNA in serum was detected by RT-PCR assay. The immunohistochemical assays of liver tissue were performed with HGV monocoloned antibody (McAb)expressed from the region of HGV NS5 nucleic acid sequence. The clinical and pathological data of 52 patients with hepatitis G were discussed. In animal experiment,the Chinese Rhesus monkeys were infected with the serum of a patient with HGV infection. And the dynamic changes in serology and liver histology of animals were observed.RESULTS One hundred and fifty-four patients with HGVRNA positive were selected from 1552 patients with various kinds of hepatitis. Of 154 patients with HGV infection, 52 were infected with HGV only, which accounted for 33.8% (52/154) and 102 with positive HGVRNA were super-infected with other hepatitis viruses,which accounted for 66.2% (102/154). The clinical and pathological observation showed that the acute and chronic hepatitis could be induced by HGV. The slight abnormality of transaminases ALT and AST in serum of monkeys lasted nearly 12 months and histological results showed a series of pathological changes.CONCLUSION HGV is a hepatotropic virus and has pathogenicty.

关键词： hepatitis virus G pathogenicity

High density lipoprotein 3 inhibits oxidized low density lipoprotein-induced apoptosis via promoting cholesterol efflux in RAW264.7 cells

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Acta Pharmacologica Sinica 2006年第2期27卷 151-157页

作者： Pei JIANG~(2,5) Peng-ke YAN~(2,5) Jian-xiong CHEN~4 Bing-yang ZHU~2 Xiao-yong LEI~2 Wei-dong YIN~(3,6) Duan-fang LIAO~(2,3,6) ~2Division of Pharmacoproteomics.institute of Pharmacy and Pharmacology,Nanhua University,Hengyang 421001,China,~3School of Life Science and Technology,Nanhua University,Hengyang 421001,China,~4Department of pathology,Vanderbilt University Medical Center Nashville.TN 37232.USA Division of Pharmacoproteomics Institute of Pharmacy and Pharmacology Nanhua University Hengyang China Department of pathology Vanderbilt University Medical Center Nashville USA School of Life Science and Technology Nanhua University Hengyang China

Aim:To investigate the protective effect of high density lipoprotein 3（HDL3）onoxidized low density lipoprotein（ox-LDL）-induced apoptosis in RAW264.7 cells,Methods:RAW264.7 cells were exposed to 50 mg/L ox-LDL for various durationsup to 48h,and apoptosis was detected using Hoechst 33258 staining and flowcytometric *** cholesterol levels were detected by high performanceliquid chromatography,cholesterol efflux was determined by Tritium labeling,andthe cellular lipid droplets were assayed by oil red O ***:Treatmentwith 50mg/L ox-LDL for 12,24,and 48h increased the apoptotic rate of RAW264,7 cells in a time-dependent *** peak apoptotic rate（47.7%）was observedafter 48h ***3at various concentrations（50mg/L,100mg/L,and 200mg/L）inhibited the ox-LDL（50mg/L for 48h）-mediated apoptosis that was accom-panied by an increased rate of intracellular cholesterol efflux,and decreased totalcholesterol levels in cells in a concentration-dependent *** ofcholesterol efflux by brefeldin decreased the protective effect of HDL3on ox-LDL-induced *** of the cholesterol efflux effected by another choles-terol acceptor,β-cyclodextrin,led to a dramatic decrease in the apoptotic rate ***:HDL3antagonizes ox-LDL-induced apoptosis in RAW264.7cells,through reducing the accumulation of toxic cholesterol.

关键词： cholesterol efflux high density lipoprotein-3 apoptosis oxidized low density lipoprotein

水稻OsWRKY89启动子紫外线-B反应元件区的鉴定

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生物化学与生物物理进展 2010年第6期37卷 671-677页

作者：赵长江郝中娜王海华陈旭君郭泽建中国农业大学农业部植物病理学重点开放实验室中国农业大学植物病理学系北京100193 浙江省农业科学院植物保护与微生物研究所杭州310021 湖南科技大学生命科学学院湘潭411201

植物需要利用太阳光能进行光合作用,因而不可避免地受到紫外线-B(UV-B)辐射的影响.为了鉴定水稻WRKY转录因子OsWRKY89基因启动子中的UV-B反应相关元件,分析了转启动子不同缺失片段与gus融合基因的水稻幼苗,发现在该启动子中存在UV-B反... 详细信息

植物需要利用太阳光能进行光合作用,因而不可避免地受到紫外线-B(UV-B)辐射的影响.为了鉴定水稻WRKY转录因子OsWRKY89基因启动子中的UV-B反应相关元件,分析了转启动子不同缺失片段与gus融合基因的水稻幼苗,发现在该启动子中存在UV-B反应元件,位于基因翻译起始位点上游-1213～-1188之间的25bp区域,碱基序列为AAGATCTACCATTGCTCTATAGCTT.结合OsWRKY89和UV-B诱导上调表达基因启动子序列分析发现,该元件区在水稻UV-B反应基因启动子上具有高度的保守性,而且与已知保守的光反应元件位置邻近,表明该区域在水稻UV-B反应的转录调控中可能具有重要功能.

关键词：水稻启动子紫外线-B 反应元件

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