High density lipoprotein 3 inhibits oxidized low density lipoprotein-induced apoptosis via promoting cholesterol efflux in RAW264.7 cells
High density lipoprotein 3 inhibits oxidized low density lipoprotein-induced apoptosis via promoting cholesterol efflux in RAW264.7 cells作者机构：Division of Pharmacoproteomics Institute of Pharmacy and Pharmacology Nanhua University Hengyang China Department of Pathology Vanderbilt University Medical Center Nashville USA School of Life Science and Technology Nanhua University Hengyang China
出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))
年 卷 期：2006年第27卷第2期
页 面：151-157页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学]
基 金：国家自然科学基金 国家973计划 湖南省自然科学基金
主 题：cholesterol efflux high density lipoprotein-3 apoptosis oxidized low density lipoprotein
摘 要：Aim:To investigate the protective effect of high density lipoprotein 3（HDL3）onoxidized low density lipoprotein（ox-LDL）-induced apoptosis in RAW264.7 cells,Methods:RAW264.7 cells were exposed to 50 mg/L ox-LDL for various durationsup to 48h,and apoptosis was detected using Hoechst 33258 staining and flowcytometric *** cholesterol levels were detected by high performanceliquid chromatography,cholesterol efflux was determined by Tritium labeling,andthe cellular lipid droplets were assayed by oil red O ***:Treatmentwith 50mg/L ox-LDL for 12,24,and 48h increased the apoptotic rate of RAW264,7 cells in a time-dependent *** peak apoptotic rate（47.7%）was observedafter 48h ***3at various concentrations（50mg/L,100mg/L,and 200mg/L）inhibited the ox-LDL（50mg/L for 48h）-mediated apoptosis that was accom-panied by an increased rate of intracellular cholesterol efflux,and decreased totalcholesterol levels in cells in a concentration-dependent *** ofcholesterol efflux by brefeldin decreased the protective effect of HDL3on ox-LDL-induced *** of the cholesterol efflux effected by another choles-terol acceptor,β-cyclodextrin,led to a dramatic decrease in the apoptotic rate ***:HDL3antagonizes ox-LDL-induced apoptosis in RAW264.7cells,through reducing the accumulation of toxic cholesterol.
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