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A pCA-based method for ancestral informative markers selection in structured populations

Science China(Life sciences) 2009年第10期52卷 972-976页

作者： ZHANG Feng1,3. ZHANG Lei1,3.& DENG Hong-Wen1,2,3.1 Key Laboratory of biomedical Information Engineering of Ministry of Education, institute of Molecular Genetics, School of Life Science and Technology, Xi’an Jiaotong university, Xi’an 710049, China 2 institute of Bioscience and Biotechnology, School of Science, beijing Jiaotong university, beijing 100044, China 3.Departments of Orthopedic Surgery and Basic medical Science, School of Medicine, university of Missouri-Kansas City, Kansas City, MO 64108, USA 1. Key Laboratory of biomedical Information Engineering of Ministry of Education Institute of Molecular Genetics School of Life Science and Technology Xi’an Jiaotong University Xi’an 710049 China 3. Departments of Orthopedic Surgery and Basic medical Science School of Medicine University of Missouri-Kansas City Kansas City MO 64108 USA 2. institute of Bioscience and Biotechnology School of Science Beijing Jiaotong University Beijing 100044 China

Identification of population structure can help trace population histories and identify disease genes. Structured association (SA) is a commonly used approach for population structure identification and association map.ing. A major issue with SA is that its performance greatly depends on the informa-tiveness and the numbers of ancestral informative markers (AIMs). present major AIM selection meth-ods mostly require prior individual ancestry information, which is usually not available or uncertain in practice. To address this potential weakness, we herein develop a novel approach for AIM selection based on p.inciple component analysis (pCA), which does not require prior ancestry information of study subjects. Our simulation and real genetic data analysis results suggest that, with equivalent AIMs, pCA-based selected AIMs can significantly increase the accuracy of inferred individual ancestries compared with traditionally randomly selected AIMs. Our method can easily be applied to whole genome data to select a set of highly informative AIMs in population structure, which can then be used to identify potential population structure and correct possible statistical biases caused by population stratification.

关键词： population structure p.inciple component analysis ancestral informative markers

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preliminary study on the experimental mice model of chronic thromboembolism pulmonary hypertension

Preliminary study on the experimental mice model of chronic ...

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中国生理学会第23.全国会员代表大会暨生理学学术大会

作者： Ran MIAO~1,Jie LIU~2,Hai-Mei SUN~3.Wang WANG~2,Jun WANG~(2,*),Chen WANG~(1,*) 1 beijing institute of Respiratory Medicine,Research Center of beijing Chao-Yang Hospital,{3.,beijing, 100020,China 2 Department of physiology,Basic medical College,{3.,beijing,100069,China 3.Department of Histology and Embryology,Basic medical College,{3.,beijing,100069,China

＜正＞Objective:Many questions remain regarding the pathogenesis,diagnosis and treatment of chronic thromboembolic pulmonary hypertension in *** probe into these questions of this disorder,we aim to find an appropriate way to establish the mice model of chronic thromboembolism pulmonary hypertension（CTEpH） with simple manipulation,high success and feasibility,low cost and similar to human pathophysiology as well. Methods:C57BL/6 mice were randomly divided into model group（n=8）,sham group（n=9） and tranexamic acid （AMCA） group（n=8）.pulmonary thromboembolism（pTE） was established by injecting autologous blood thrombi to jugular vein once a week,totally four *** order to prevent thrombolysis,tranexamic acid（20 mg/kg）,a fibrinolytic inhibitor with the effect of competitive inhibition of plasminogen activation was used once a day during the entire experiment *** app.inted time of the beginning of the sixth week,right ventricular systolic pressure（RVSp） of mice was measured via direct cardiac puncture using a closed-chest technique and the heart and lung tissues were *** right ventricular hypertrophy index（RVHI） was *** established models were evaluated by morphological observation of lung tissue,histological section of hematoxylin-eosin（HE） staining to evidence thrombi in ***:（1） RVSp was elevated in model group compared to sham group （19.3.±0.763. vs 17.18±0.693. mmHg,p＜0.05） with 12.8%increase of the pressure.（2） RVHI was higher in model group than sham group[（29.60±1.6900）%vs（22.95±0.8120）%,p＜0.01]with 29.0%rising of this index. （3. No obvious thrombi were detected in the histological section of lung tissues of model group mice at the sixth ***,compared with sham group,it was found that medial smooth muscle layer was thicken,the cavity became narrow or nearly closed and inflammatory cell infiltration in pulmonary arterioles of the model group, which suggested that pulmonary vascular remodeling ***:The

关键词： chronic thromboembolism pulmonary hypertension animal model mice

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Effects of peripheral nociception on neural activities of motor cortex in rats:in vivo multi-channel recordings

Effects of peripheral nociception on neural activities of mo...

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中国生理学会第23.全国会员代表大会暨生理学学术大会

作者： Chang-Ming WANG~1,Lei YANG~1,Jun CHEN~(1,2,*) 1 institute for.biomedical sciences of p.in,capital medical university,beijing 100069,China 2 institute for.biomedical sciences of p.in and institute for Functional Brain Disorders,Tangdu Hospital,Fourth Military medical university,Xi''an 71003.,China

＜正＞Objective:The present study was designed to investigate the effects of subcutaneous saline or bee venom injection on responses of the motor cortical neurons and those of behaviors in conscious,behaving ***:We examined the motor cortical neuronal activation with an in vivo multi-channel recording technique before and after saline or bee venom was delivered subcutaneously（s.c.） into the plantar surface of the hind paw contralateral to the recording ***:A total of 81 single neurons were recorded in the motor *** first analyzed the spatial sequence *** saline-induced phasic p.in state,29（3.%） neurons showed significant excitatory responses,whereas 19（23.） displayed significant inhibitory *** bee venom-induced tonic p.in state,52（64%） indicated significant excitatory responses,however,7（9%） showed inhibitory *** then analyzed the temperoal sequence *** terms of saline-induced phasic nociception,a sharp.increase of spike discharges at the start of saline injection was *** terms of bee venom-induced tonic p.in state,the increase of spike discharges was during the initial half hour after *** venom ***:The present result,a microcosmic view of the brain p.in-related responses,accorded well with the results of neuroimaging studies,a macroscopic view of the brain p.in-related responses,indicating the role of the motor cortex in the information processing of p.in.

关键词： bee venom neural ensemble coding pattern tonic nociception phasic nociception motor cortex

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传染病多点触发智慧化监测预警系统关键技术专家共识

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中华医学杂志 2024年第32期104卷 2995-3009页

作者：中国医学科学院北京协和医学院群医学及公共卫生学院北京大学公共卫生学院中国疾病预防控制中心传染病管理处四川大学华西公共卫生学院中国科学院地理科学与资源研究所首都医科大学附属北京地坛医院中华医学会公共卫生分会中国医学科学院北京协和医学院群医学及公共卫生学院北京大学公共卫生学院中国疾病预防控制中心传染病管理处四川大学华西公共卫生学院中国科学院地理科学与资源研究所首都医科大学附属北京地坛医院中华医学会公共卫生分会

有效的传染病监测预警系统是公共卫生安全体系的重要组成部分, 对预防与控制传染病暴发流行至关重要, 提升监测预警能力是推动疾病预防控制事业高质量发展的当务之急。专家组结合公共卫生与预防医学、临床医学、管理学、数据科学、计算... 详细信息

有效的传染病监测预警系统是公共卫生安全体系的重要组成部分, 对预防与控制传染病暴发流行至关重要, 提升监测预警能力是推动疾病预防控制事业高质量发展的当务之急。专家组结合公共卫生与预防医学、临床医学、管理学、数据科学、计算机科学等多学科专家的研究成果与实践经验, 经过反复多轮专家研讨, 形成了传染病多点触发智慧化监测预警系统关键技术专家共识。本共识内容主要包括传染病多点触发智慧化监测预警系统的相关概念和定义, 关键技术框架, 多渠道监测预警数据来源、获取与治理, 预警方法分类, 多点触发智慧化监测预警关键流程及路径, 多点触发预警及综合研判, 预警信号响应以及预警效果评价等方面, 以期为传染病多点触发智慧化监测预警系统建设及应用提供技术参考。

关键词：传染病监测预警多点触发智慧化

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Spatio-temporal pattern analysis of single-trial EEG signals recorded during visual object recognition

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Science China(Information sciences) 2011年第12期54卷 2519-2527页

作者： WANG ChangMing 1 , HU Xiaoping 3., YAO Li 1,2 , XIONG Shi 2 & ZHANG JiaCai 2 1 National Key Laboratory of Cognitive Neuroscience and Learning, beijing Normal university, beijing 100875, China 2 College of Information Science and Technology, beijing Normal university, beijing 100875, China 3.biomedical Imaging Technology Center, Emory university/Georgia institute of Technology, Atlanta 3.3.2, USA 1. National Key Laboratory of Cognitive Neuroscience and Learning Beijing Normal University Beijing 100875 China3. Biomedical Imaging Technology Center Emory University/Georgia Institute of Technology Atlanta 30322 USA2. College of Information Science and Technology Beijing Normal University Beijing 100875 China

Object discrimination is a fundamental cognitive function for human brains. In this study, we utilized an analysis approach for single-trial electroencephalography (EEG) to analyze the spatio-temporal activation patterns of visual objects processing in human brains, and attempted to apply it to discriminate different visual objects. The spatial patterns were respectively extracted from scalp EEG and the reconstructed cortical sources, while the experiment participants were perceiving 4 different categories of visual objects (faces, buildings, cats and cars). By classifying the patterns extracted from single-trial EEG, the presented visual objects could be discriminated by a computer, and the classification accuracies may provide a quantitative index to evaluate the spatial differences of the brain activations related to different visual objects. Our results demonstrated that the spatial patterns on both the scalp and the sources levels resulted in higher classification accuracies than chance rate. We also examined the classification results using temporally non-overlap.ing time intervals of different event-related potential (ERp) components. The temporal changes of classification accuracies may reflect the temporal distribution of the discriminative information in the EEG responses. The present pattern extraction and classification methods may compose a computational model for single-trial EEG data analysis in investigations of the spatio-temporal activation patterns for object recognition. These spatio-temporal patterns in EEG may be useful to identifying the discriminative spatial areas and time stages to improve the accuracies and efficiencies of object discrimination. In addition, investigating and utilizing humans'cognitive mechanisms of object recognition may improve computers' processing efficiency of visual information.

关键词： EEG spatio-temporal pattern object recognition single-trial analysis visual information processing

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Influence of expressed TRAIL on biophysical properties of the human leukemic cell line Jurkat

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Cell Research 2004年第2期14卷 161-168页

作者： Kai CHEN~(1,*), Dan LI~(1,*), Yu Hui JIANG~1, Wei Juan YAO~1, Xin Juan WANG~2, Xiao Chao WEI~2, Jing GAO~3. Li De XIE~1, Zong Yi YAN~4, Zong Yao WEN~(1**), Shu CHIEN~5 ~1Hemorheology Center, Department of Biophysics, School of Basic medical sciences, peking university, {3., 100083. China. ~2Department of Biochemical and Molecular Biology, School of Basic medical sciences, peking university, {3., 100083. China. ~3.epartment of Urology, medical Center, New York university, USA. ~4Department of Mechanics and Engineering Science, peking university, {3. 100871, China. ~5Department of Bioengineering and Whitaker institute of {13. Engineering, university of California, San Diego, La Jolla, CA 92093.0412, USA 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 2Department of Biochemical and Molecular Biology School of Basic Medical Sciences Peking University Beijing 100083 China. 2Department of Biochemical and Molecular Biology School of Basic Medical Sciences Peking University Beijing 100083 China. 3.epartment of Urology Medical Center New York University USA. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 4Department of Mechanics and Engineering Science Peking University Beijing 100871 China. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 5Department of Bioengineering and Whitaker institute of biomedical Engineering University of California San Diego La Jolla CA 92093-0412 USA

The cDNA fragment of human TRAIL (TNF-related apoptosis inducing ligand) was cloned into RevTet-On, a Tetregulated and high-level gene expression system. The gene expression system was constructed in a human leukemic cell line: Jurkat. By using RevTet-On TRAIL gene expression system in Jurkat as a cell model, we studied the influence of TRAIL gene on the changes of cellular apoptosis before and after the TRAIL gene expression, which was induced by adding tetracycline derivative doxycycline (Dox). The results indicated that the cellular apoptosis ratio was largely dependent on the TRAIL gene expression level. Moreover, it was found that the apoptosis-inducing TRAIL could cause significant changes in the biophysical properties of Jurkat cells. The cell surface charge density decreased, the membrane fluidity declined, the elastic coefficients K_I increased, and the proportion of α-helix in membrane protein secondary structure decreased. Thus, the apoptosis-inducing TRAIL gene caused significant changes on

关键词： TRAIL Tet gene expression system Jurkat apoptosis.

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Development of silicon-based microelectrode array

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Science China(Technological sciences) 2009年第8期52卷 2391-2395页

作者： LIN JianHui1,2,3. WU XiaoMing1,2,3.4, HUANG pengSheng1,2, FENG Lei5, REN TianLing1,2 & LIU LiTian1,2 1 institute of Microelectronics, Tsinghua university, beijing 100084, China 2 Tsinghua National Laboratory for Information Science and Technology, beijing 100084, China 3.Yangtze Delta Region institute of Tsinghua university, Jiaxing 3.4050, China 4 State Key Laboratory of Transducer Technology, Chinese Academy of sciences, Shanghai 200050 China 5 Department of biomedical Engineering, Tsinghua university, beijing 100084, China 1. institute of Microelectronics Tsinghua University Beijing 100084 China 2. Tsinghua National Laboratory for Information Science and Technology Beijing 100084 China 3. Yangtze Delta Region institute of Tsinghua university Jiaxing 314050 China 4. State Key Laboratory of Transducer Technology Chinese Academy of Sciences Shanghai 200050 China 5. Department of biomedical Engineering Tsinghua University Beijing 100084 China

This paper introduces in details a kind of silicon-based microelectrode array. MEMS (micro-electromechanical system) technology is used in the fabrication of the microelectrode array, which is designed to perform signal recording and electrical stimulation for nerves in neural engineering. A simple fabrication process is developed. An improved model of microelectrodes is brought forward and successfully validated by the excellent match between circuit simulations and electrical measurements, including both magnitude and phase of microelectrode impedance. Compared with the simple one that is usually used, the improved model is believed to be an advance and more accurate. This modeling helps to improve the design of microelectrodes and understand the behavior of interface between electrode and cell. Furthermore, the microelectrode is proved to be a feasible tool for researches in neural engineering by successfully recording neural activities of sciatic nerve of a bullfrog.

关键词： microelectrode array MEMS neural engineering

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The protection of astrocyte against rotenone-induced dopaminergic neuron injury

The protection of astrocyte against rotenone-induced dopamin...

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中国神经科学学会第六届学术会议暨学会成立十周年庆祝大会

作者： Wei Ling-rong, Yang Hui beijing institute for Neuroscience capital {3. of medical sciences, beijing

＜正＞parkinson disease （pD） is a common neurodegenerative disorder characterized by the progressive loss of the dopaminergic neurons in the substantia nigra pars compacta （SNpc）. The pathological process is associated with a astrocyte response. However, the function of astrocytes neuron degenerative process is still a mysterious.

关键词： astrocyte neuron neuroprotection oxidative stress

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Metabotropic glutamate receptor subtype 1 and 5 mediate opposite effects on p.in-related spatial and temporal synaptic plasticity in rat hippocampal formation:Multi-electrode array recordings

Metabotropic glutamate receptor subtype 1 and 5 mediate oppo...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Yan WANG~1,Ming-Gang LIU~2,Dan LV~2,Rui-Rui WANG~1,Xue-Feng CHEN~1,Fu-Kang ZHANG~2,Xiu-Yu CUI~2,Jun CHEN~(1,2,*) 1 institute for.biomedical sciences of p.in and institute for Functional Brain Disorders,Tangdu Hospital,The Fourth Military medical {3.,Xi''an 71003.,China 2 institute for.biomedical sciences of p.in,capital medical university,beijing 100069,China

＜正＞Objective It has been revealed that peripheral persistent nociception results in spatial and temporal synaptic plasticity in rat hippocampal formation（HF）,however,the underlying mechanisms are still *** the present report,we present a line of evidence implicating opposing roles of metabotropic glutamate receptor（mGluR） subtype 1 and 5 in mediation of p.in-related spatial and temporal synaptic plasticity in the *** Inhibitors targeting mGluRl and mGluR5 were used,*** and temporal components of entorhinal-hippocampal synaptic connections and efficacy of rats were recorded by using a newly developed 64-channels（8×8）multi-electrode array technique（Med64 system）.Results（1）pharmacological inhibition of mGluR5 activation,at the plateau phase of the long-term potentiation（LTp）,effectively decreased p.in-enhanced hippocampal long-term potentiation（LTp） maintenance,while similar blockade of mGluRl resulted in an even higher level of potentiation.（2）The mGluR subtype-related difference was also detected when analyzing effects of these two drugs on the spatial amplification of p.in,with no clear involvement of mGluR5 and a negative modulation exerted by *** These findings suggest differential and even contradictory roles of mGluRl and mGluR5 in p.in-associated spatial and temporal plasticity of synaptic *** importantly,this maybe the first demonstration for a subtype-linked functional separation/opposition regarding roles of group I mGluRs in higher levels of abnormal p.in processing in the HF.

关键词： persistent p.in hippocampal formation synaptic plasticity multi-electrode array metabotropic glutamate receptor

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Genomic research for important pathogenic bacteria in China

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Science China(Life sciences) 2009年第1期52卷 50-63页

作者： YANG RuiFu1,GUO XiaoKui2,YANG Jian3.JIANG YongQiang1,pANG Bo4,CHEN Chen5,YAO YuFeng2,QIN JinHong2 & LI QingTian2 1 Laboratory of Analytical Microbiology,State Key Laboratory of pathogen and Biosecurity,beijing institute of Microbiology and Epidemiology,beijing 100071,China 2 Department of {3. Microbiology and parasitology,institutes of {3. sciences,Shanghai Jiao Tong university School of Medicine,Shanghai 200025,China 3.State Key Laboratory for Molecular Virology and Genetic engineering,institute of pathogen Biology,Chinese Academy of {3. sciences,beijing 100176,China 4 State Key Laboratory for Infectious Disease prevention and Control,institute for Communicable Disease Control and prevention,Chinese Center for Disease Control and prevention,beijing 102206,China 5 beijing Genomics institute at Shenzhen,Shenzhen 518083.China 1. Laboratory of Analytical Microbiology State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing 100071 China 2. Department of medical Microbiology and parasitology Institutes of Medical Sciences Shanghai Jiao Tong University School of Medicine Shanghai 200025 China 3. State Key Laboratory for Molecular Virology and Genetic engineering Institute of Pathogen Biology Chinese Academy of Medical Sciences Beijing 100176 China 4. State Key Laboratory for Infectious Disease prevention and Control Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China 5. beijing Genomics institute at Shenzhen Shenzhen 518083 China

Rapid accumulation of bacterial genomic data offered an unprecedented opportunity to understand bacterial biology from a holistic view of *** can thus closely look at the way in which a pathogen is evolved,and these data has been applied to molecular epidemiology and microbial forensics,and screening of novel diagnostic,vaccine and drug *** newly developed high-throughput low-cost sequencing technologies,such as 454,Solexa and SOLiD,will promote the acquisition and application of genomic data in new research areas that we dared not imagine previously,such as the metagenomics of human gastric-intestinal tract,for better and comprehensive understanding of human health and disease.

关键词： genome pathogenic bacteria sequencing application

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