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Molecular Plant 2015年第11期8卷 1651-1664页

作者： Nils Kleinboelting Gunnar Huep ingo appelhagen Prisca Viehoever Yong Li Bernd Weisshaar Center for Biotechnology & Department of Biology Bielefeld University Universitaetsstrasse 25 33615 Bielefeld Germany Department of Medicine IV University Hospital Freiburg Berliner Allee 29 79110 Freiburg Germany

Transformation by Agrobacterium tumefaciens, an important tool in modern plant research, involves the integration of T-DNA initially present on a plasmid in agrobacteria into the genome of plant cells. The process of attachment of the agrobacteria to plant cells and the transport of T-DNA into the cell and further to the nucleus has been well described. However, the exact mechanism of integration into the host＇s DNA is still unclear, although several models have been proposed. During confirmation of T-DNA insertion alleles from the GABI-Kat collection of Arabidopsis thaliana mutants, we have generated about 34 000 sequences from the junctions between inserted T-DNA and adjacent genome regions. Here, we describe the evaluation of this dataset with regard to existing models for T-DNA integration. The results suggest that integration into the plant genome is mainly mediated by the endogenous plant DNA repair machinery. The observed integration events showed characteristics highly similar to those of repair sites of double- strand breaks with respect to microhomology and deletion sizes. In addition, we describe unexpected integration events, such as large deletions and inversions at the integration site that are relevant for correct interpretation of results from T-DNA insertion mutants in reverse genetics experiments.

关键词： T-DNA integration Agrobacterium tumefaciens double-strand break repair Arabidopsis thaliana

Sex-specific life-history trait expression in hybrids of a cave- and surface-dwelling fish (Poecilia mexicana, Poecilidae)

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Current Zoology 2024年第4期70卷 421-429页

作者： Rüdiger Riesch Luis R.Arriaga ingo Schlupp Department of Biological Sciences Royal Holloway University of LondonEghamTW20 OEXUK CEFE CNRSUnivMontpellierEPHEIRDMontpellierFrance Department of Biology University of OklahomaNormanOK 73019USA

Evaluating the fitness of hybrids can provide important insights into genetic differences between species or diverging *** focused on surface-and cave-ecotypes of the widespread Atlantic molly Poecilia mexicana and raised F1 hybrids of reciprocal crosses to sexual maturity in a common-garden *** were reared in a fully factorial 2 x 2 design consisting of lighting(light ***)and resource availability(high *** food).We quantified survival,ability to realize their full reproductive potential(i.e.,completed maturation for males and 3 consecutive births for females)and essential life-history *** to the performance of pure cave and surface fish from a previous experiment,F1s had the highest death rate and the lowest proportion of fish that reached their full reproductive *** also uncovered an intriguing pattern of sex-specific phenotype expression,because male hybrids expressed cave molly life histories,while female hybrids expressed surface molly life *** results provide evidence for strong selection against hybrids in the cave molly system,but also sug-gest a complex pattern of sex-specific(opposing)dominance,with certain surface molly genes being dominant in female hybrids and certain cavemollygenes beingdominant in malehybrids.

关键词： dominance life-history evolution local adaptation postzygotic isolation selection against hybrids.

Multitarget Thiol-Activated Tetrapyridyl Gold(Ⅲ)Complexes for Hypoxic Cancer Therapy

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CCS Chemistry 2024年第3期6卷 783-797页

作者： Xue-Quan Zhou Selda Abyar Imma Carbo-Bague Lan Wang Sebastian Türck Maxime ASiegler Uttara Basu ingo Ott Rongfang Liu Adriaan PIJzerman Sylvestre Bonnet Institute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate School(SIGS)Tsinghua UniversityShenzhen 518055 Leiden Institute of Chemistry Leiden University2333 CC Leiden Department of Chemistry Simon Fraser UniversityBurnabyBritish Columbia V5A 1S6 Institute of Medicinal and Pharmaceutical Chemistry Technische Universität Braunschweig38106 Braunschweig Department of Chemistry Johns Hopkins UniversityBaltimoreMaryland 21218 Division of Drug Discovery&Safety Leiden Academic Centre for Drug ResearchLeiden University2333 CC Leiden

Gold complexes have emerged as promising anticancer metallodrugs due to their efficient thioredoxin reductase(TrxR)inhibition,which disturbs the redox balance of cancer ***,in this model,the role of the ligand(s)coordinated to gold is often *** this work,we present a series of tetrapyridyl Au(Ⅲ)complexes that exhibit thiol-induced release of a Au(Ⅰ)ion and a tetrapyridyl *** formation of a free Au(Ⅰ)center is responsible for the expected TrxR ***,the released ligand,which was visible in cells due to its intense blue fluorescence,showed excellent binding properties to the hERG potassium ***,these ligands ended up in the lysosomes,resulting in significant lysosome ***,the Au(Ⅲ)complexes presented in this work showed broad-spectrum anticancer properties,both in hypoxic 2D monolayers and 3D tumor *** suggest that the interaction of the released Au(Ⅰ)center and the tetrapyridyl ligand with two different protein targets may combine into prodrugs that overcome hypoxia-induced drug deactivation.

关键词： anticancer Au(Ⅲ)complexes enzyme inhibtion potassium channel affinity lysosome damage cell imaging prodrug

Gallai-Ramsey Number for the Union of Stars

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Acta Mathematica Sinica,English Series 2022年第8期38卷 1317-1332页

作者： Ya Ping MAO Zhao WANG Colton MAGNANT ingo SCHIERMEYER School of Mathematics and Statistics Qinghai Normal UniversityXining 810008P.R.China College of Science China Jiliang UniversityHangzhou 310018P.R.China Department of Mathematics Clayton State UniversityMorrowGA30260USA Technische Universit?t Bergakademie Freiberg Institut für Diskrete Mathematik und Algebra09596 FreibergGermany

Given a graph G and a positive integer k,define the Gallai–Ramsey number to be the minimum number of vertices n such that any k-edge coloring of Kn contains either a rainbow(all different colored)triangle or a monochromatic copy of *** this paper,we obtain exact values of the Gallai–Ramsey numbers for the union of two stars in many cases and bounds in other *** work represents the first class of disconnected graphs to be considered as the desired monochromatic subgraph.

关键词： Ramsey theory Gallai-Ramsey number union of stars

Tempering of exhausted T cells to comprehend their adaptive response for suitable clinical translation

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Cellular & Molecular Immunology 2023年第12期20卷 1401-1402页

作者： Amit Sharma ingo G.H.Schmidt-Wolf Department of Integrated Oncology Center for Integrated Oncology(CIO)BonnUniversity Hospital BonnBonnGermany

Although cancer immunotherapy has taken center stage,exhaustion of T cells is undeniably one of the major problems preventing it from being fully successful[1].Certainly,reversal of T-cell exhaustion or dysfunction is of great importance for developing new strategies and restoring immune ***,counterarguments about the adverse effect of reversing T-cell exhaustion(leading to overactivation of T cells and cytotoxicity)and even their irreversible properties have been quite common in scientific *** more to be discovered about the reasons for T-cell failure,a better understanding of the molecular factors/determinants[2,3]that shape/remodel“heterogeneous”dysfunctional T cells in cancer makes clear ***,two recent publications in Nature immunology have described the intriguing perspective of T-cell exhaustion[4,5].Of interest,Vignali et *** on the hypoxia-driven CD39-dependent suppressor function of exhausted T cells,whereas Saadey et *** restoring the epigenetic balance of TGFβ1/BMP signaling in exhausted T cells to unlock responsiveness to immune checkpoint blockade therapy.

关键词： irreversible exhaustion publications

Optimized Three-Dimensional Cardiovascular Magnetic Resonance Whole Heart Imaging Utilizing Non-Selective Excitation and Compressed Sensing in Children and Adults with Congenital Heart Disease

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Congenital Heart Disease 2023年第3期18卷 279-294页

作者： ingo Paetsch Roman Gebauer Christian Paech Frank-Thomas Riede Sabrina Oebel Andreas Bollmann Christian Stehning Jouke Smink ingo Daehnert Cosima Jahnke Department of Electrophysiology Heart Center Leipzig at University of LeipzigLeipzigGermany Department of Pediatric Cardiology Heart Center Leipzig at University of LeipzigLeipzigGermany Philips Research Laboratories HamburgGermany Philips Medical Systems BestThe Netherlands

Background:In congenital heart disease(CHD)patients,detailed three-dimensional anatomy depiction plays a pivotal role for diagnosis and therapeutical decision ***,the present study investigated the applicability of an advanced cardiovascular magnetic resonance(CMR)whole heart imaging approach utilizing nonselective excitation and compressed sensing for anatomical assessment and interventional guidance of CHD patients in comparison to conventional dynamic CMR ***:86 consecutive pediatric patients and adults with congenital heart disease(age,1 to 74 years;mean,35 years)underwent CMR imaging including a freebreathing,ECG-triggered 3D nonselective SSFP whole heart acquisition using compressed SENSE(nsWHcs).Anatomical assessability and signal intensity ratio(SIR)measurements were compared with conventional dynamic 3D-/4D-MR ***:The most frequent diagnoses were partial anomalous pulmonary venous drainage(17/86,20%),transposition of the great arteries(15/86,17%),tetralogy of Fallot(12/86,14%),and a single ventricle(7/86,8%).Image quality of nsWHcs was rated as excellent/good in 98%of *** resulted in a reliable depiction of all large thoracic vessels(anatomic assessability,99%–100%)and the proximal segments of coronary arteries and coronary sinus(>90%).nsWHcs achieved a homogenously distributed SIR in all cardiac cavities and thoracic vessels without a significant difference between pulmonary and systemic circulation(10.9±3.5 and 10.6±3.4;p=0.15),while 3D angiography showed significantly increased SIR for targeted ***-targeted circulation(PA-angiography,15.2±8.1 vs.5.8±3.6,p<0.001;PV-angiography,7.0±3.9 vs.17.3±6.8,p<0.001).Conclusions:The proposed nsWHcs imaging approach provided a consistently high image quality and a homogeneous signal intensity distribution within the pulmonary and systemic circulation in pediatric patients and adults with a wide spectrum of congenital heart *** enabled detailed anatomi

关键词： Cardiovascular magnetic resonance imaging congenital heart disease whole heart imaging nonselective SSFP compressed SENSE MR angiography

Microstructure analyses and phase-field simulation of partially divorced eutectic solidification in hypoeutectic Mg-Al Alloys

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Journal of Magnesium and Alloys 2022年第6期10卷 1672-1679页

作者： Joo-Hee Kang Jiwon Park Kyung Song Chang-Seok Oh Oleg Shchyglo ingo Steinbach Korea Institute of Materials Science Changwon 51508Republic of Korea Interdisciplinary Centre for Advanced Materials Simulation Ruhr-University Bochum44801 BochumGermany

In this study the partially divorced eutectic microstructure ofα-Mg andβ-Mg17Al12was investigated by electron backscatter diffraction,transmission electron microscopy,and phase-field modeling in hypoeutectic Mg-Al *** orientation relationships between the individual eutecticαgrains,eutecticβphase,and primaryαgrains were *** the amount of eutectic morphology is primarily determined by the Al content,the in-depth microstructure analyses and the phase-field simulation suggest non-interactive nucleation and growth of eutecticαphase in theβphase grown on the interdendritic primaryα***,phase-field simulations showed a preferred nucleation sequence where theβphase nucleates first and subsequently triggers the nucleation of eutecticαphase at the movingβphase solidification front,which supports the microstructural analysis results.

关键词： Mg-Al alloy Partially divorced eutectic Solidification Electron backscatter diffraction Phase-Field modeling

Identification and mapping of Rpi-blb4 in diploid wild potato species Solanum bulbocastanum

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The Crop Journal 2023年第6期11卷 1828-1835页

作者： Jie Li Amanpreet Kaur Brian Harrower Miles Armstrong Daolong Dou Xiaodan Wang ingo Hein Department of Plant Pathology and MOA Key Lab of Pest Monitoring and Green Management College of Plant ProtectionChina Agricultural UniversityBeijing 100193China The James Hutton Institute InvergowrieDundee DD25DAUK University of Dundee Dundee DD14HNUK Crop Research Centre TeagascOakParkCarlow R93 XE12Ireland College of Plant Protection Academy for Advanced Interdisciplinary StudiesNanjing Agricultural UniversityNanjing 210095JiangsuChina

More than 170 years after causing the potato famine in Ireland,late blight is still considered one of the most devastating crop *** potato breeding efforts depend on natural sources of resistance to protect crops from the rapidly evolving late blight pathogen,Phytophthora *** have identified and mapped a novel broad-spectrum disease resistance gene effective against *** from the wild,diploid potato species Solanum *** resistance gene enrichment sequencing(dRenSeq)was used to confirm the uniqueness of the identified *** and GenSeq-based mapping of the resistance,referred to as Rpi-blb4,alongside recombinant screening,positioned the locus responsible for the resistance to potato chromosome *** interval spans approximately 2.3 Mb and corresponds to the DM reference genome positions of 11.25 and 13.56 Mb.

关键词： Renseq Disease resistance Late blight Solanum bulbocastanum

Tracking the phase transformation and microstructural evolution of Sn anode using operando synchrotron X-ray energy-dispersive diffraction and X-ray tomography

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Journal of Energy Chemistry 2023年第1期76卷 429-437,I0011页

作者： Kang Dong Fu Sun Andre Hilger Paul H.Kamm Markus Osenberg Francisco Garcia-Moreno ingo Manke Institute of Applied Materials Helmholtz-Zentrum Berlin fur Materialien und EnergieHahn-Meitner-Platz 1Berlin 14109Germany Institute of Materials Science and Technology Technische Universitat BerlinHardenbergstraβe 36Berlin 10623Germany Qingdao Institute of BioEnergy and Bioprocess Technology Chinese Academy of Sciences189 Songling RoadQingdao 266101ShandongChina

Tin(Sn)holds great promise as an anode material for next-generation lithium(Li)ion batteries but suffers from massive volume change and poor cycling *** clarify the dynamic chemical and microstructural evolution of Sn anode during lithiation and delithiation,synchrotron X-ray energydispersive diffraction and X-ray tomography are simultaneously employed during Li/Sn cell *** intermediate Li-Sn alloy phases during de/lithiation are identified,and their dynamic phase transformation is unraveled which is further correlated with the volume variation of the Sn at particle-and ***,we find that the Sn particle expansion/shrinkage induced particle displacement is anisotropic:the displacement perpendicular to the electrode surface(z-axis)is more pronounced compared to the directions(x-and y-axis)along the electrode *** anisotropic particle displacement leads to an anisotropic volume variation at the electrode level and eventually generates a net electrode expansion towards the separator after cycling,which could be one of the root causes of mechanical detachment and delamination of electrodes during long-term *** unraveled chemical evolution of Li-Sn and deep insights into the microstructural evolution of Sn anode provided here could guide future design and engineering of Sn and other alloy anodes for high energy density Li-and Na-ion batteries.

关键词： Sn anode Li-Sn phase transformation X-ray tomography Operando X-ray diffraction Anisotropic displacement Digital volume correlation(DVC)

Ballistic tests on hot-rolled Ti-6Al-4V plates:Experiments and numerical approaches

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Defence Technology（防务技术） 2023年第8期26卷 39-53页

作者： Alexander Janda Benjamin James Ralph Yael Demarty Marcel Sorger Stefan Ebenbauer Aude Prestl ingo Siller Martin Stockinger Helmut Clemens Department of Materials Science Montanuniversitat LeobenFranz Josef-Straße 188700 LeobenAustria Department Product Engineering Montanuniversitat LeobenFranz Josef-Straße 188700 LeobenAustria French-German Research Institute of Saint-Louis 5 Rue du General Cassagnou68300 Saint-LouisFrance voestalpine BOEHLER Bleche GmbH&Co KG Boehler-Gasse 18680MürzzuschlagAustria

Superior ballistic performance and the lightweight character of Ti alloys are considered as main reasons for their use in armour applications against a broad spectrum of ballistic threats,***,fragment or blast *** dynamic loading caused by typical penetrators is characterized by high strain rates,only specific test methods allow a closer investigation of the respective material *** the present study,quasi-static and dynamic compression tests as well as ballistic tests were conducted on a twophase a+βalloy Ti-6Al-4V(in m%)manufactured by ***-deformation heat treatments,influencing microstructure and mechanical properties were applied in order to compare three different microstructural configurations:as-rolled,mill-annealed and ***,on the one hand,ballistic tests were employed for the determination of the ballistic limit velocity v_(50),compression tests,on the other hand,delivered essential input parameters for the application of the Johnson-Cook constitutive model in a finite element simulation of the impact *** comparison of experimental results to simulation results was supplemented by means of microstructural characterization of tested samples with the focus set on the prevalently observed deformation and damage mechanisms,as for example adiabatic shearing.

关键词： Ti-6Al-4V Ballistic performance Split Hopkinson pressure bar FE simulation Adiabatic shear bands Intermetallic phase