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Light-stabilized FHA2 suppresses miRNA biogenesis through interactions with DCL1 and HYL1

Molecular Plant 2021年第4期14卷 647-663页

作者： Seung Jun Park Suk Won choi Gu Min Kim Christian Moller Hyun-Sook Pai seong Wook Yang Department of Systems Biology College of Life Science and BiotechnologyYonsei UniversitySeoul 120749Korea EDepartment of Systems Biology Institute of Life Science and BiotechnologyYonsei UniversitySeoul 120-749Korea Department of Plant and Environnental Sciences Faculty of ScienceUniversity of CopenhagenThorvaldsensvei 40DK-1871 FrederiksbergCopenhagenDenmark Pohang University of Science and Technology(POSTECH) 77 Cheongam-RoNam-GuPohangGyeongbuk37673.Korea These authors contributed equally to this article.

The precise regulation of microRNA(miRNA)biogenesis is crucial for plant development,which requires core microprocessors and many fine tuners to coordinate their miRNA processing activity/specificity in fluctuating cellular *** de-etiolation,light triggers a dramatic accumulation of core microprocessors and primary miRNAs(pri-miRNAs)but decreases pri-miRNA processing activity,resulting in relatively constant miRNA *** mechanisms underlying these seemingly contradictory regulatory changes remain *** this study,we identified forkhead-associated domain 2(FHA2)as a light-stabilized suppressor of miRNA *** found that FHA2 deficiency increased the level of mature miRNAs,accompanied by a reduction in pri-miRNAs and target *** assays showed that FHA2 associates with the core microprocessors DCL1,HYL1,and SE,forming a complex to suppress their pri-miRNA processing *** analyses revealed that FHA2 promotes HYL1 binding but inhibits the binding of DCL1-PAZ-RNase-RNA-binding domains(DCL1-PRR)to miRNAs,whereas FHA2 does not directly bind to these ***,we found that FHA2 protein is unstable in the dark but stabilized by light during ***,disruption of FH A led to defects in light-triggered changes in miRNA expression and reduced the survival rate of deetiolated seedlings after prolonged light ***,these data suggest that FHA2 is a novel light-stabilized suppressor of miRNA biogenesis and plays a role in fine-tuning miRNA processing during de-etiolation.

关键词： miRNA biogenesis Suppressor of the microprocessor miRNA-biogenetic inconsistency

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Synthesis of Al-doped ZnO Nanorods via Microemulsion Method and Their Application as a CO Gas Sensor

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Journal of Materials Science & Technology 2015年第6期31卷 639-644页

作者： Sang Kyoo Lim seong Hui Hong Sung-Ho Hwang Won Mi choi Soonhyun Kim Hyunwoong Park Min Gi Jeong Division of Nano & Bio Technology Daegu Gyeongbuk Institute of Science and Technology(DGIST) School of Energy Engineering Kyungpook National University Department of Advanced Organic Materials Engineering Yeungnam University

Aluminum doped ZnO(AZO) nanorods were synthesized by microemulsion method with different types of *** phase and the morphology of the above nanorods were investigated by scanning electron microscopy(SEM) and X-ray diffraction(XRD).SEM observations showed that the ZnO nanorods had diameters around about 50—200 nm and lengths up to several *** CO gas sensing properties of the AZO nanorods were tested at operating temperatures of 200,300,350 and 400 ℃.It was found that AZO nanorods based sensor exhibited the highest sensitivity to CO at 350℃.

关键词： Zinc oxide Nanorod Microemulsion Aluminum CO and g

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Engineered NiCo-LDH nanosheets- and ZnFe_(2)O_(4) nanocubes-decorated carbon nanofiber bonded mats for high-rate asymmetric supercapacitors

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Green Energy & Environment 2022年第6期7卷 1228-1240页

作者： Jae-Gyoung seong Tae Hoon Ko Danyun Lei Woong-Ki choi Yun-Su Kuk Min-Kang Seo Byoung-Suhk Kim Department of Organic Materials&Fiber Engineering Jeonbuk National University567 Baekje-daeroDeokjin-guJeonju-siJeollabuk-do54896Republic of Korea Department of BIN Convergency Technology Jeonbuk National University567 Baekje-daeroDeokjin-guJeonju-siJeollabuk-do54896Republic of Korea Korea Institute of Carbon Convergence Technology Jeonju54852Republic of Korea Department of Environmental Biology and Water Engineering College of Urban ConstructionWuchang Shouyi UniversityWuhan430064China

In this work,we have prepared the hierarchically nanostructured core–shell NiCo layered double hydroxide(NiCo-LDH)nanosheets-and ZnFe_(2)O_(4) nanocubes-decorated polyacrylonitrile(PAN)/pitch-based carbon nanofibers(PPCNs)webs(NiCo-LDH@PPCNs as cathode and ZnFe_(2)O_(4)@PPCNs as anode materials)with the bonded network structure by a facile and scalable hydrothemal ***,the low-cost pitch with lower softening point(~90℃)as co-precursor was utilized to produce the PAN/pitch-based carbon nanofibers(PPCNs)with enhanced electrical *** obtained PPCNs with pitch content of 30%(PP30CNs)electrode material delivered higher specific capacitance(~67 F g^(-1))than that(~48 F g^(-1))of the PAN-based carbon nanofibers(PCNs)at 1 A g^(-1),due to the increased electrical conductivity and lower interfacial charge transfer resistance(RCT)of~0.16 ***,the NiCo-LDH-decorated PP30CNs(NiCoLDH@PP30CNs)as cathode material showed superior specific capacitance of 1162 F g^(-1) at 1.0 A g^(-1) and ultra-high retention rate of 91.56%at 10 A g^(-1).The ZnFe_(2)O_(4)@PP30CNs as anode material also showed higher specific capacitance of 282 F g^(-1) at 1 A g^(-1) and good rate capability with capacitance retention of 56.73%at 10 A g^(-1).The as-fabricated asymmetric NiCo-LDH@PP30CNs//ZnFe_(2)O_(4)@PP30CNs hybrid supercapacitor device delivered a specific capacitance of~98 F g^(-1) at 1 A g^(-1) and excellent capacitance retention of~88%after 5000 charge–discharge cycles.

关键词： Carbon nanofiber Pitch Asymmetric Rate capability Supercapacitor

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Purifying behavior of photocatalytic TiO_2 anodized in nitrate ion containing solution

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中国有色金属学会会刊：英文版 2009年第4期19卷 1027-1030页

作者： Jin-Wook choi seong-Eun LEE Byung-Gwan LEE Yong-Soo JEONG Han-Jun OH Choong-Soo CHI School of Advanced Materials Engineering Kookmin University Korea Institute of Materials Science Department of Materials Science Han seo University

Mesoporous titanium dioxide films were fabricated on titanium plates by micro-arc oxidation *** increase the photocatalytic activity of the films,NH4NO3 was added to the H2SO4 solution,and anodizing was carried out at high voltages using a DC power *** crystal structure,chemical composition,surface morphology and the optical property of the films were investigated by XPS,XRD,UV-VIS spectroscopy and *** photocatalytic activity of the films was evaluated by the decomposition of aniline blue,and the activity of the films for the degradation turned out to be improved by the additives to the electrolyte *** enhanced photocatalytic activity might result from the increased porosity and nitrate ion incorporation into the anodic films by micro arcing,and thereby the TiO2 layer might exhibit an improved absorption property for the visible light.

关键词：二氧化钛硝酸溶液盐离子催化行为氧化钛薄膜光催化活性 H2SO4溶液净化

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Formation behavior of anodic TiO_2 nanotubes in fluoride containing electrolytes

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中国有色金属学会会刊：英文版 2009年第4期19卷 842-845页

作者： Byung-Gwan LEE Jin-Wook choi seong-Eun LEE Yong-Soo JEONG Han-Jun OH Choong-Soo CHI School of Advanced Materials Engineering Kookmin University Korea Institute of Materials Science Department of Materials Science Han seo University

TiO2 nanotube layers can be formed with titanium in the electrolytes containing fluoride by electrochemical *** role of fluoride ion,the crystallinity of the anodic oxide,and the chemical state were *** results show the anodic film is composed of oxide and a little amount of *** presence of F- ions leads to chemical dissolution of Ti oxide layer and prevents hydroxide ***,chemical dissolution rate increases with increasing the fluoride content in the range of 0-2%(in mass fraction) because F- ions in electrolyte attack the interface and allow the ions of the electrolyte to easily penetrate into the *** as-anodized TiO2 nanotubes exhibit an amorphous *** treated nanotubes are composed of mixtures of the anatase and rutile phases.

关键词： TiO2纳米管阳极氧化处理氟离子电解质行为二氧化钛化学溶解电化学方法

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SnO2@Co3O4 hollow nano-spheres for a Li-ion battery anode with extraordinary performance

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Nano Research 2014年第8期7卷 1128-1136页

作者： Won-Sik Kim Yoon Hwa Hong-Chan Kim Jong-Hyun choi Hun-Joon Sohn seong-Hyeon Hong Department of Materials Science and Engineering and Research Institute of Advanced Materials Seoul National University Seoul151-744 Republic of Korea

SnO2@Co3O4 hollow nano-spheres have been prepared using the template-based sol-gel coating technique and their electrochemical performance as an anode for lithium-ion battery （LIB） was investigated. The size of synthesized hollow spheres was about 50 nm with the shell thickness of 7-8 nm. The fabricated SnO2@Co3O4 hollow nano-sphere electrode exhibited an extraordinary reversible capacity （962 mAh-g-1 after 100 cycles at 100 mA-g-1）, good cyclability, and high rate capability, which was attributed to the Co-enhanced reversibility of the Li20 reduction reaction during cycling.

关键词： hollow sphere lithium-ion battery anode SnO2 Co3O4

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Current status of genetic transformation technology developed in cucumber(Cucumis sativus L.)

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Journal of Integrative Agriculture 2015年第3期14卷 469-482页

作者： WANG Shun-li seong Sub Ku YE Xing-guo HE Cong-fen Suk Yoon Kwon Pil Son choi Key Laboratory of Biology and Genetic Improvement of Horticultural Crops Ministry of Agriculture/Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Department of Oriental Pharmaceutical Development Nambu University Institute of Crop Science Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement School of Sciences Beijing Technology and Business University Plant Systems Engineering Research Center

Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.

关键词： Cucumis sativus L. plant regeneration genetic transformation positive selection system

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Soil texture affects the conversion factor of electrical conductivity from 1:5 soil-water to saturated paste extracts

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Pedosphere 2022年第6期32卷 905-915页

作者： Bo-seong SEO Young-Jae JEONG Nu-Ri BAEK Hyun-Jin PARK Hye In YANG Se-In PARK Woo-Jung choi Department of Rural and Bio-systems Engineering(BK21) Chonnam National UniversityGwangju 61186(Republic of Korea) Department of Renewable Resources University of AlbertaEdmontonAlberta T6G 2E3(Canada) Max Planck Institute for Biogeochemistry Jena 07745(Germany) National Institute of Environmental Research Incheon 22689(Republic of Korea) AgriBio Institute of Climate Change Management Chonnam National UniversityGwangju 61186(Republic of Korea)

Electrical conductivity(EC)of soil-water extracts is commonly used to assess soil ***,its conversion to the EC of saturated soil paste extracts(ECe),the standard measure of soil salinity,is currently required for practical *** many regression models can be used to obtain ECe from the EC of soil-water extracts,the application of a site-specific model to different sites is not straightforward due to confounding soil factors such as soil *** study was conducted to develop a universal regression model to estimate a conversion factor(CF)for predicting EC_(e) from EC of soil-water extracts at a 1:5 ratio(EC_(1:5)),by employing a site-specific soil texture(i.e.,sand content).A regression model,CF=8.9105e^(0.0106sand)/1.2984(r^(2)=0.97,P<0.001),was developed based on the results of coastal saline soil surveys(n=173)and laboratory experiments using artificial saline soils with different textures(n=6,sand content=10%-65%)and salinity levels(n=7,salinity=1-24 dS m^(-1)).Model performance was validated using an independent dataset and demonstrated that EC_(e) prediction using the developed model is more suitable for highly saline soils than for low saline *** feasibility of the regression model should be tested at other *** soil factors affecting EC conversion factor also need to be explored to revise and improve the model through further studies.

关键词： coastal saline soils electrical conductivity prediction reclaimed tideland regression model sand content saturation percentage soil salinity

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Fast preparation of photopolymerized monolithic columns for capillary electrochromatography

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Journal of Central South University of Technology 2008年第2期15卷 176-182页

作者：龚文君许光日张毅军张裕平 choi seong-ho LEE Kwang-pill Henan Institute of Science and Technology Department of Chemistry Hannam UniversityDaejonSouth Korea Department of Chemistry Graduate SchoolKyungpook National UniversityDaegu702-701South Korea

Photopolymerized sol-gel(PSG) columns were prepared using methacryloxypropyltrimethoxysilane as the monomer,toluene as the porogen and hydrochloric acid as the *** different photoinitiators such as benzoin methyl ether,Irgacure 819,Irgacure 1700 and Irgacure 1800 were comparatively used in the reaction solution in the presence and absence of sodium dodecyl *** above eight solutions were respectively irradiated at 365 nm for 5?10 min in each capillary(75 μm inside diameter) to prepare the porous monolithic sol-gel column by a one-step,in situ,*** chromatographic behavior of the eight PSG columns were comparatively studied,all of which exhibit reversed-phase *** these columns,several neutral compounds,namely thiourea,benzene,toluene,ethyl benzene,biphenyl and naphthalene can be separated from mixtures with a largest column efficiency of 74 470 plate/column for *** of sodium dodecyl sulfate in the polymerization process has a significant influence on the morphology and migration time.

关键词： electrochromatography monolithic columns photopolymerized sol-gel aromatic hydrocarbons

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Retroperitoneal desmoplastic small round cell tumor:Pediatric patient treated with multimodal therapy

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World Journal of Gastroenterology 2009年第33期15卷 4212-4214页

作者： Yong Seok Kim seong Jae Cha Yoo Shin choi Beom Gyu Kim Sung Jun Park In Taik Chang Department of Surgery College of MedicineChung-Ang University

A desmoplastic small round cell tumor (DSRCT) is a rare, aggressive mesenchymal neoplasm. Although a DSRCT can develop at various sites, the intraabdominal site is the most common location. These tumors are found most commonly among young adolescents and the prognosis is extremely poor. Multimodal treatment with surgery, chemotherapy and radiotherapy is very important for these rare cases, and this treatment can improve patient survival. In this report, we describe the case of an 8-year-old boy diagnosed with DSRCT located in the retroperitoneal space. The patient has undergone surgical resection and adjuvant chemoradiation therapy, and is currently alive without disease recurrence.

关键词： Retroperitoneum Desmoplastic small round cell tumor Multimodal therapy

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