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Expressions of Sphingosine-1-phosphate (S1P) Receptors, Sphingosine Kinases in Malignant Bone and Soft Tissue Tumors, and The role of Sphingosine Kinase-1 in Growth of MFH Cell Lines

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Journal of Cancer Therapy 2011年第2期2卷 288-294页

作者： Shin-ichiro kishimoto Toshihiro Akisue Kenta kishimoto Hitomi Hara Masaya Imabori Yoshiyuki Okada Naomasa Fukase Teruya Kawamoto Ikuo Fujita Takuya Fujimoto Masahiro Kurosaka Department of Orthopaedic Surgery Kobe University Graduate School of Medicine Kusunoki-cho Chuo-ku Kobe JAPAN

Sphingolipids are ubiquitous components of cell membranes. Their metabolites ceramide, sphingosine, and sphingosine-1-phosphate (S1P) have important physiological functions, including regulation of cell growth and survival. S1P is generated by phosphorylation of sphingosine catalyzed by sphingosine kinase-1 (SPHK1). The purpose of this study is to explore the roles of S1P, S1P receptors, and sphingosine kinases in malignant musculoskeletal tumors. Twenty-one tumor samples (7 liposarcomas, 3 chondrosarcomas, 6 osteosarcomas, 5 MFH) obtained at open biopsy, and four human MFH cell lines (Nara H, Nara F, TNMY1, GBS-1) were used. We examined the mRNA expression of S1P receptors by RT-PCR, and the expression levels of SPHK by Real-time PCR. We used 4 MFH cell lines to analyze SPHK1 proteins by Western blotting. SPHK1 siRNA was transfected into MFH cell lines by lipofection method. Cell proliferation (control and transfected with siRNA) was assayed using WST-8 (Cell Counting Kit-8) assay. All high grade malignant tumors expressed S1P1, S1P2, S1P3 receptors, whereas the expression of S1P1 receptor was detected in 50% of low-grade malignant tumors, S1P2 receptor in 30%, and S1P3 in 50%. No statistically significant difference was found in the expression level of SPHK1 between high-grade and low-grade malignant tumors by Real-time PCR. By results of Western blotting, proteins of SPHK1 were expressed in all MFH cell lines. In MFH cell lines, transfection with SPHK1 siRNA oligonucleotides resulted in approximately 50 to 80% suppression of SPHK1 mRNA expression as determined by real-time PCR. Down-regulation of SPHK1 with small interfering RNA significantly reduced SPHK1 protein levels by Western blotting. Knock down of SPHK1 expression significantly decreased cell proliferation of all MFH cells. These results suggest that the expression of S1P receptors may play an important role for cell proliferation and may correlate with histologic grade in malignant bone and soft tissue t

关键词： Sarcoma Sphingosine-1-Phosphate S1p Receptor Sphingosine Kinase MIB-1 MFH

The emerging role of Arid5a in cancer: A new target for tumors

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Genes & Diseases 2023年第3期10卷 813-824页

作者： Kishan Kumar Nyati Tadamitsu kishimoto Laboratory of Immune Regulation World Premier International Immunology Frontier Research CenterOsaka UniversityOsaka 5650871Japan

AT-rich interactive domain 5a (Arid5a) is a member of the arid family of proteins, which contain a helix-turn-helix domain and an ability to bind to nucleic acids. Current evidence suggests that Arid5a performs dual functions as a transcription factor and an RNA-binding protein in immune, nonimmune, and/ or tumor cells depending on its cellular localization. The contribution of Arid5a to the development of inflammation, autoimmunity, and obesity through its transcriptional and posttranscriptional regulatory functions has broadly been reviewed. Recent studies have indeed revealed an association of Arid5a with cancers, including breast, pancreatic, colorectal, and lung cancers and glioma. Notably, Arid5a affects various aspects of cellular homeostasis, including invasion, metastasis, epithelial-to-mesenchymal transition, immune evasion, adipogenesis and M1-like tumor-associated macrophage (TAM)-to-M2-like TAM transition. This review aims to summarize current knowledge of Arid5a from a cancer perspective and highlights recent advances in Arid5a-related cancer research. This review may improve the understanding of Arid5a-mediated molecular mechanisms and their relevance to cancers.

关键词： Arid5a Cancer Inflammation Metastasis Transcriptional and posttanscriptional regulation Tumor micr oenvir onment

Effect of microstructure on outer surface roughening of magnesium alloy tubes in die-less mandrel drawing

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Journal of Magnesium and Alloys 2022年第10期10卷 2730-2744页

作者： Takuma kishimoto Peihua Du Tsuyoshi Furushima Department of Mechanical and Biofunctional Systems Institute of Industrial ScienceThe University of Tokyo4-6-1 KomabaMeguroTokyo 153-8505Japan

The crystal orientation and outer surface roughening of magnesium alloy tubes were evaluated to clarify the effect of the mandrel on the microstructure and outer surface roughness in die-less mandrel drawing. Locally heated ZM21 tubes with an outer diameter of 6.0 mm and an inner diameter of 3.8 mm were drawn with and without a mandrel. The outer surface roughness and crystal orientation were evaluated in the same measurement area. The results indicated that the outer surface becomes rougher in the die-less mandrel drawing than in die-less drawing for a given outer circumferential strain. The outer surface roughness developed when there was large difference in the pyramidal slip system Schmid factor. Therefore, the slip deformation of the pyramidal slip system seems to be mainly responsible for the outer surface roughening in the die-less mandrel drawing. Furthermore, the crystal grain with the {2110} crystal plane vertical to the normal direction of outer surface had a larger Schmid factor than the other crystal grains. The large number of crystal grains with the {2110} crystal plane in the die-less mandrel drawing is one of the reasons that the outer surface roughness develops more in the die-less mandrel drawing than in die-less drawing for a given outer circumferential strain. These results will contribute significantly to the development of fabrication process of the microtube with high surface quality, which prevents rapid corrosion of biomedical applications.

关键词： Tube forming Die-less drawing Surface roughness Crystal plasticity Magnesium alloy Electron backscatter diffraction

C-Crossed Products by R, III： Traces

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Acta Mathematica Sinica,English Series 2011年第7期27卷 1259-1282页

作者： Akitaka kishimoto Hokkaido University Department of Mathematics Sapporo 0600810 Japan

When a flow on a C＊-algebra has KMS states we study traces on the associated crossed product to show that they are determined by KMS states under a mild assumption on the flow.

关键词： Flow crossed product trace KMS state

Severe hand contracture treated by external fixation after proximal row carpectomy and awake tenolysis after general anesthesia: A case report

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Chinese Journal of Traumatology 2022年第1期25卷 54-58页

作者： Takuya Tsumura Taiichi Matsumoto Mutsumi Matsushita Katsuma kishimoto Hayao Shiode Hiroshi Murakami Department of Orthopedic Surgery Kurashiki Central Hospital1-1-1 MiwaKurashiki710-0052Japan

Wide-awake local anesthesia has many *** describe a new method to use wide-awake local anesthesia with more flexibility.A 32-year-old man with a severe right-hand contracture after an iatrogenic tourniquet accident during an anterolateral thigh flap for a partial hand amputation underwent contracture release using external fixation after proximal row carpectomy and subsequent *** performed most of the tenolysis procedure under general anesthesia and the final stage with an intra-operative assessment of active finger movement and dissection under local *** regained his grip strength 2.5 years *** anesthesia is useful to treat a surgical site with extensive hard scars,whereas local anesthesia is useful for adjusting tension in an awake *** indication for wide-awake surgery is yet to be established;our method of combining general and local anesthesia in the tenolysis procedure illustrates the possibilities in expanding this method.

关键词： General anesthesia Ilizarov technique Tendons Carpectomy

AI powered, automated discovery of polymer membranes for carbon capture

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npj Computational Materials 2023年第1期9卷 966-976页

作者： Ronaldo Giro Hsianghan Hsu Akihiro kishimoto Toshiyuki Hama Rodrigo F.Neumann Binquan Luan Seiji Takeda Lisa Hamada Mathias B.Steiner IBM Research Av.República do Chile330CEP 20031-170 Rio de JaneiroRJBrazil IBM Research 7-7Shin-KawasakiSaiwai-KuKawasakiKanagawa 212-0032Japan IBM Research 1101 Kitchawan Road PO Box 218Yorktown HeightsNY 10598-0218USA

The generation of molecules with artificial intelligence(AI)or,more specifically,machine learning(ML),is poised to revolutionize materials *** applications range from development of potent drugs to efficient carbon capture and separation ***,existing computational discovery frameworks for polymer membranes lack automated training data creation,generative design,and physical performance validation at meso-scale where complex properties of amorphous materials *** methodological gaps are less relevant to the ML design of individual molecules such as the monomers which constitute the building blocks of ***,we report automated discovery of complex materials through inverse molecular design which is informed by meso-scale target features and process *** have explored the multi-scale discovery regime by computationally generating and validating hundreds of polymer candidates designed for application in post-combustion carbon dioxide ***,we have validated each discovery step,from training dataset creation,via graph-based generative design of optimized monomer units,to molecular dynamics simulation of gas permeation through the polymer *** the latter,we have devised a representative elementary volume(REV)enabling permeability simulations at about 1000×the volume of an individual,ML-generated monomer,obtaining quantitative *** discovery-to-validation time per polymer candidate is on the order of 100 h using one CPU and one GPU,offering a computational screening alternative prior to lab validation.

关键词： monomer latter elementary

Wave-Number Spectral Characteristics of Drift Wave Micro-Turbulence with Large-Scale Structures

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Plasma Science and Technology 2011年第3期13卷 297-301页

作者：李继全 Y.kishimoto Graduate School of Energy Science Kyoto University 611-0011 Gokasho Uji Japan

Wave-number spectral characteristics of drift wave micro-turbulence with large-scale structures （LSSs） including zonal flows （ZFs） and Kelvin-Holmheltz （KH） mode are investigated based on three dimensional gyrofluid simulations in a slab geometry. The focus is on the property of the wave-number spectral scaling law of the ambient turbulence under the back reaction of the self-generated LSSs. A comparison of the spectral scaling laws between ion/electron temperature gradient （ITG/ETG） driven turbulences is presented. It is shown that the spectral scaling of the ITG turbulence with robust ZFs is fitted well by an exponential-law function （Φ^2/2）E∝e^-λkx in kx and a power-law one in （Φ^2/2）p∝ky^-β in ky. However, the ETG turbulence is characterized by a mixing Kolmogorov-like power-law and exponential-law （Φ^2/2）E∝e-λkx＇yk^-3x,y/（1 ＋ k^2x,y）^2 scaling for both kx and ky spectra due to the ZFs and KH mode dynamics, with λ and β the slope index factors. The underlying physical mechanism is understood as the spectral scattering caused by the back-reaction of the LSSs on the ambient turbulence. These findings may provide helpful guideline to diagnose the plasma fluctuations and flow structures in experiments.

关键词： spectral scaling law zonal flows micro-turbulence gyrofluid simulation

A QUANTITATIVE MODEL FOR INTRADAY STOCK PRICE CHANGES BASED ON ORDER FLOWS

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Journal of Systems Science & Complexity 2014年第1期27卷 208-224页

作者： LIMeng HUI Xiaofeng ENDO Misao kishimoto Kazuo School of Management Harbin Institute of Technology Harbin 150001 China. Socio-economic Research Center Central Research Institute of Electric Power Industry Tokyo 100-8126 Japan. Graduate School of Systems Information Engineering University of Tsukuba Ibaraki-ken 305-8571 Japan.

This paper proposes a double Markov model of the double continuous auction for describing intra-day price changes. The model splits intra-day price changes as the repetition of one tick price moves and assumes order arrivals are independent Poisson random processes. The dynamic process of price formation is described by a birth-death process of the double M/M/1 server queue corresponding to the best bid/ask. The initial depths of the best bid and ask are defined as different constants depending on the last price change. Thus, the price changes in the model follow a first-order Markov process. As the initial depth of the best bid/ask is originally larger than that of the opposite side when the last price is down/up, the model may explain the negative autocorrelations of the price of the best bid/ask. The estimated parameters are based on the real tick-by-tick data of the Nikkei 225 futures listed in Osaka Stock Exchanges. The authors find the model accurately predicts the returns of Osaka Stock Exchange average.

关键词：定量模型股价价格变化证券交易所流量定单马尔可夫模型马尔可夫过程

EFFECT OF TRIAXIAL STRESS CONSTRAINT ON THE DEFORMATION AND FRACTURE OF POLYMERS

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Acta Mechanica Sinica 2002年第5期18卷 480-493页

作者：王铁军 Kikno kishimoto Mitsuo Notomi Department of Engineering Mechanics Xi'an Jiaotong UniversityXi'an 710049China Department of Mechanical and Control Engineering Tokyo Institute of TechnologyTokyo 152-8552Japan Department of Mechanical Engineering Meiji UniversityKawasaki-shiKanagawa 214-8571Japan

One purpose of this paper is to give a brief overview on the research status of deformation,fracture and toughening mechanisms of polymers,including experimental,theoretical and numerical *** is on the more recent progresses of micromechanics of rubber particle cavitation and crazing,and the de- velopment of fracture criteria for ductile polymers. The other purpose is to study the effect of triaxial stress constraint on the deforma- tion and fracture behavior of ***(PC),acrylonitrile-butadiene- styrene(ABS)and PC/ABS alloy are considered in this investigation.A series of circumferentially blunt-notched bars are used to experimentally generate different tri- axial stress *** fracture surfaces of specimens with different notch radius are examined by scanning electron microscope(SEM)to study the fracture and tough- ening mechanisms of polymer *** is shown that the triaxial stress constraint has a significant effect on the deformation,fracture and toughening of PC,ABS and PC/ABS *** will also discuss the extent to which a micromechanies criterion proposed by the first author can serve as a fracture criterion for ductile polymers. A new ductile fracture parameter is emphasized,which can be employed to evaluate the fracture ductility of *** state independence of the parameter for the PC,ABS and PC/ABS alloy has been experimentally verified.

关键词： deformation fracture toughening cavitation crazev micromechanics PC ABS polymer alloy