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Sequence-length variation of mtDNA HVS-I C-stretch in Chinese ethnic groups

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journal of Zhejiang University-Science b(biomedicine & biotechnology) 2009年第10期10卷 711-720页

作者： Feng CHEN Yong-hui DANG Chun-xia yaN yan-ling LIU ya-jun DENG David j. R. FULTON Teng CHEN Department of Forensic Medicine School of Medicine Xi'an Jiaotong University Xi'an 710061 China Ministry of Education Key Laboratory of Environment and Genes Related to D&eases Xi 'an Jiaotong University Xi'an 710061 China beijing Genomics Institute Chinese Academy of Sciences Beijing 100029 China Medical College of Georgia 1459 Laney Walker Blvd Augusta Georgia 30912 USA

The purpose of this study was to investigate mitochondrial DNA (mtDNA) hypervariable segment-I (HVS-I) C-stretch variations and explore the significance of these variations in forensic and population genetics studies. The C-stretch sequence variation was studied in 919 unrelated individuals from 8 Chinese ethnic groups using both direct and clone sequencing ap-proaches. Thirty eight C-stretch haplotypes were identified, and some novel and population specific haplotypes were also detected. The C-stretch genetic diversity (GD) values were relatively high, and probability (P) values were low. Additionally, C-stretch length heteroplasmy was observed in approximately 9% of individuals studied. There was a significant correlation (r=-0.961, Pbetween the expansion of the cytosine sequence length in the C-stretch of HVS-I and a reduction in the number of up-stream adenines. These results indicate that the C-stretch could be a useful genetic maker in forensic identification of Chinese populations. The results from the Fst and dA genetic distance matrix, neighbor-joining tree, and principal component map also suggest that C-stretch could be used as a reliable genetic marker in population genetics.

关键词： Mitochondrial DNA (mtDNA) Clone sequencing Length heteroplasmy Population genetics

益心泰对慢性心力衰竭大鼠线粒体分裂蛋白Fis1、Mff的影响

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中国实验方剂学杂志 2024年第4期30卷 143-151页

作者：刘承鑫魏佳明王子焱施敏袁惠唐云李雅郭志华湖南中医药大学第一中医临床学院中医学院药学院慢病中医智能诊断与治未病湖南省普通高等学校重点实验室互联网+慢病中医诊治与养生智能化应用研究生联合培养基地长沙410208

目的:研究益心泰对慢性心力衰竭大鼠线粒体分裂蛋白的干预作用及其机制。方法:60只SD大鼠随机抽取10只作为假手术组,剩余50只采用结扎左冠状动脉前降支法制备心肌梗死后心力衰竭大鼠模型。将造模成功的大鼠随机分为模型组、益心泰低、... 详细信息

目的:研究益心泰对慢性心力衰竭大鼠线粒体分裂蛋白的干预作用及其机制。方法:60只SD大鼠随机抽取10只作为假手术组,剩余50只采用结扎左冠状动脉前降支法制备心肌梗死后心力衰竭大鼠模型。将造模成功的大鼠随机分为模型组、益心泰低、中、高剂量组(1.4、2.8、5.6 g·kg^(-1))、曲美他嗪组(10 mg·kg^(-1)),各给药组予相应剂量药物灌胃,模型组和假手术组给予等体积的生理盐水灌胃,连续给药28 d。采用酶联免疫吸附测定法(ELISA)检测大鼠血清氨基末端b型利钠肽(NT-pro bNP)、b型利钠肽(bNP)、腺嘌呤核苷三磷酸(ATP)含量;彩色多普勒超声成像仪检测心功能指标;苏木素-伊红(HE)染色、马松(Masson)染色法观察心脏病理形态学改变,使用image j软件计算胶原容积分数(CVF);透射电镜观察心肌细胞超微结构变化;原位末端标记法(TUNEL)染色检测心肌组织细胞凋亡率;蛋白免疫印迹法(Western blot)检测心肌组织线粒体外膜线粒体分裂蛋白1(Fis1)、线粒体分裂因子(Mff)表达。结果:与假手术组比较,模型组血清NT-pro bNP、bNP含量显著升高(PbNP、bNP含量降低(P<0.05),ATP含量升高(P<0.05),益心泰低、中、高剂量组及曲美他嗪组LVEF、LVFS升高(P<0.01),LVIDd、LVIDs均降低(P<0.01),各剂量益心泰治疗组及曲美他嗪组心肌炎症损伤减轻、纤维化得到改善,CVF降低(P<0.01),各剂量益心泰治疗组及曲美他嗪组心肌线粒体结构得到改善,各剂量益心泰治疗组及曲美他嗪组心肌细胞凋亡指数下降(P<0.01);益心泰中、高剂量组及曲美他嗪组心肌组织Fis1、Mff蛋白表达下调(P<0.05)。结论:益心泰可改善线粒体结构、减轻心肌细胞凋亡、提升心功能,其作用机制可能与抑制心肌组织线粒体分裂蛋白Fis1、Mff表达有关。

关键词：益心泰心力衰竭线粒体动力学凋亡线粒体分裂

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Research of Power Take-off System for “Sharp Eagle Ⅱ” Wave Energy Converter

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China Ocean Engineering 2019年第5期33卷 618-627页

作者： YE Yin WANG Kun-lin YOU ya-ge SHENG Song-wei Guangzhou Institute of Energy Conversion Chinese Academy of Sciences Guangzhou 510640 China Key Laboratory of Renewable Energy Chinese Academy of Sciences Guangzhou 510640 China Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development Guangzhou 510640 China

The " Sharp Eagle” device is a wave energy converter of a hinged double floating body. The wave-absorbing floating body hinges on the semi-submerged floating body structure. Under the action of wave, the wave-absorbing floating body rotates around the hinge point, and the wave energy can be converted into kinetic energy. In this paper, the power take-off system of " Sharp Eagle Ⅱ” wave energy converter (the second generation of " Sharp Eagle”) was studied, which adopts the hydraulic type power take-off system. The 0-1 power generation mode was applied in this system to make the " Sharp Eagle Ⅱ” operate under various wave conditions. The principle of power generation was introduced in detail, and the power take-off system was simulated. Three groups of different movement period inputs were used to simulate three kinds of wave conditions, and the simulation results were obtained under three different working conditions. In addition, the prototype of " Sharp Eagle Ⅱ” wave energy converter was tested on land and in real sea conditions. The experimental data have been collected, and the experimental data and simulation results were compared and validated. This work has laid a foundation for the design and application of the following " Sharp Eagle” series of devices.

关键词： Sharp Eagle Wave Energy Converter power take-off power generation mode hydraulic system

Accretivity of the General Second Order Linear Differential Operator

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Acta Mathematica Sinica,English Series 2019年第6期35卷 832-852页

作者： V.G.MAZ'ya I.E.VERbITSKY Department of Mathematics Linkoping University SE-581 83 Linkoping Sweden RUDN University 6 Miklukho-Maklay St. Moscow 117198 Russia Department of Mathematics University of Missouri Columbia Missouri 65211 USA

For the general second order linear differential operator ■ with complex-valued distributional coefficients a_(jk), b_j, and c in an open set Ω ? R^n(n ≥ 1), we present conditions which ensure that-L^0 is accretive... 详细信息

关键词： Accretive differential operators complex-valued coefficients Schr?dinger operator form boundedness

挂浆对罗非鱼片预制菜品质提升及蛋白质稳定性的影响

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南方水产科学 2024年第4期20卷 11-23页

作者：吴青青黄卉郝淑贤岑剑伟魏涯相欢胡晓赵永强上海海洋大学食品学院上海201306 中国水产科学研究院南海水产研究所/农业农村部水产品加工重点实验室/国家水产品加工技术研发中心广东广州510300

以罗非鱼(Oreochromis mossambicus)鱼片为原料的酸菜鱼产品是水产类预制菜的主要品种之一。为提升鱼片在复热时的质构稳定性,探讨了挂浆对鱼片品质和鱼肉蛋白稳定性的影响。通过添加淀粉、蛋清粉、花生油降低鱼片在复热过程中的蒸煮损... 详细信息

以罗非鱼(Oreochromis mossambicus)鱼片为原料的酸菜鱼产品是水产类预制菜的主要品种之一。为提升鱼片在复热时的质构稳定性,探讨了挂浆对鱼片品质和鱼肉蛋白稳定性的影响。通过添加淀粉、蛋清粉、花生油降低鱼片在复热过程中的蒸煮损失率,提升鱼片的质构特性,优化3类物质的添加量和工艺,以解决鱼片类预制菜在复热过程中易破碎的问题。结果表明,添加淀粉、蛋清粉和花生油均能抑制罗非鱼片的水分流失,降低蒸煮损失,提升质构特性,同时对汤汁浑浊度的影响较小。提高罗非鱼片煮制品质稳定性的最佳挂浆工艺为:淀粉添加量4.4%、蛋清粉添加量3.6%、花生油添加量1.4%、腌制时间13 min。鱼片在此条件下的综合得分(16.16±0.53)接近于预测值(16.70),说明工艺稳定可靠。与未挂浆鱼片相比,优化条件下鱼片煮制的质构硬度提高了74.2%,汤汁浑浊度降低了42.7%,蛋白质的α-螺旋和β-转角结构相对含量更多,结构更稳定,肌纤维间连接的紧密性更好,食用品质显著提升。

关键词：罗非鱼片挂浆响应面质构汤汁浑浊度

P-wave contributions to b(s)→ψKπ decays in perturbative QCD approach

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Chinese Physics C 2020年第7期44卷 22-33页

作者： ya Li Zhou Rui Zhen-jun Xiao Department of Physics College of SciencesNanjing Agricultural UniversityNanjing 210095China College of Sciences North China University of Science and TechnologyTangshan 063009China Department of Physics and Institute of Theoretical Physics Nanjing Normal UniversityNanjing 210023China

We study the quasi-two-body decays b(s)ψ[K*(892)K*(1410)K*(1680)]ψKπby employing the perturbative QCD(PQCD)factorization approach,where the charmoniaψrepresents j/ψandψ(2S).The corresponding decay channels are studied by constructing the kaon-pion distribution amplitude(DA)ΦKπP,which comprises important final state interactions between the kaon and pion in the resonant *** breit-Wigner formulas are adopted to parameterize the time-like form factor FKπappearing in the kaon-pion *** SU(3)flavor symmetry breaking effect resulting from the mass difference between the kaon and pion is taken into account,which makes significant contributions to the longitudinal *** observed branching ratios and the polarization fractions of b(s)ψK*(892)ψKπare accommodated by tuning hadronic parameters for the kaon-pion *** PQCD predictions for b(s)ψ[K*(1410)K*(1680)]ψKπmodes from the same set of parameters can be tested by precise data obtained in the future from LHCb and belleⅡexperiments.

关键词： PQCD factorization approach two-meson distribution amplitudes quasi-two-body b meson decays

Macrophage regulation of graft-vs-host disease

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World journal of Clinical Cases 2020年第10期8卷 1793-1805页

作者： ya-Qun Hong bo Wan Xiao-Fan Li Fujian Institute of Hematology Fujian Provincial Key Laboratory on HematologyDepartment of HematologyFujian Medical University Union HospitalFuzhou 350000Fujian ProvinceChina Faculty of Life Sciences and Medicine King’s College LondonLondon WC1N 3BGUnited Kingdom INSERM U1160 Hospital Saint LouisUniversitéParis DiderotParis 94430France

Hematopoietic stem cell transplantation has become a curative choice of many hematopoietic malignancy,but graft-vs-host disease(GVHD)has limited the survival quality and overall survival of hematopoietic stem cell *** of the immune cells’reaction in pathophysiology of GVHD has improved,but a review on the role of macrophages in GVHD is still *** have observed that macrophage infiltration is associated with GVHD occurrence and *** this review,we summarize and analyze the role of macrophages in GVHD based on pathophysiology of acute and chronic GVHD,focusing on the macrophage recruitment and infiltration,macrophage polarization,macrophage secretion,and especially interaction of macrophages with other immune *** could conclude that macrophage recruitment and infiltration contribute to both acute and chronic *** on distinguishing pathology of acute and chronic GVHD,macrophages tend to show a higher M1/M2 ratio in acute GVHD and a lower M1/M2 ratio in chronic ***,the influence of dominant cytokines in GVHD is controversial and inconsistent with macrophage *** addition,interaction of macrophages with alloreactive T cells plays an important role in acute ***,the interaction among macrophages,b cells,fibroblasts,and CD4+T cells participates in chronic GVHD development.

关键词： Macrophage Graft-vs-host disease Hematopoietic stem cell transplantation Polarization Cytokine Regulation

抗水通道蛋白4抗体阳性视神经脊髓炎谱系疾病诊断与治疗进展

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中国现代神经疾病杂志 2024年第5期24卷 308-314页

作者：许鸿嘉舒崖清王蘋李蕃许顺良邱伟马晓宇山东大学第二医院神经内科济南250033 中山大学附属第三医院神经内科广州510630

视神经脊髓炎谱系疾病(NMOSDs)以抗水通道蛋白4(AQP4)抗体阳性NMOSDs最为多见,主要表现为反复发作的视神经炎和脊髓炎等,严重降低患者生活质量。近年来,对该病的诊断技术、诊断标志物和免疫治疗药物不断有新的认识,本文综述抗AQP4抗体阳... 详细信息

视神经脊髓炎谱系疾病(NMOSDs)以抗水通道蛋白4(AQP4)抗体阳性NMOSDs最为多见,主要表现为反复发作的视神经炎和脊髓炎等,严重降低患者生活质量。近年来,对该病的诊断技术、诊断标志物和免疫治疗药物不断有新的认识,本文综述抗AQP4抗体阳性NMOSDs的诊断技术和诊断标志物新进展以及新的治疗药物,以促进对该病的诊断与治疗新技术的理解和应用,探索更多可能的临床诊断与治疗方法。

关键词：视神经脊髓炎水通道蛋白质4 免疫疗法综述

Simultaneous changes in seed size, oil content and protein content driven by selection of SWEET homologues during soybean domestication

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National Science Review 2020年第11期7卷 1776-1786页

作者： Shoudong Wang Shulin Liu jie Wang Kengo Yokosho bin Zhou ya-Chi Yu Zhi Liu Wolf b.Frommer jian Feng Ma Li-Qing Chen Yuefeng Guan Huixia Shou Zhixi Tian State Key Laboratory of Plant Physiology and biochemistry College of Life sciences Zhejiang University State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Innovation Academy for Seed Design Chinese Academy of Sciences University of Chinese Academy of Sciences College of Resources and Environment Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology Fujian Agriculture and Forestry University Institute of Plant Science and Resources Okayama University Institute of Crop Science Anhui Academy of Agricultural Sciences Department of Plant biology School of Integrative Biology University of Illinois at Urbana-Champaign Institute for Molecular Physiology and Cluster of Excellence on Plant Sciences (CEPLAS) Heinrich Heine University of Düsseldorf FAFU-UCR joint Center for Horticultural Plant biology and Metabolomics Haixia Institute of Science and Technology Fujian Agriculture and Forestry University

Soybean accounts for more than half of the global production of oilseed and more than a quarter of the protein used globally for human food and animal feed. Soybean domestication involved parallel increases in seed size and oil content, and a concomitant decrease in protein content. However, science has not yet discovered whether these effects were due to selective pressure on a single gene or multiple genes. Here,re-sequencing data from>800 genotypes revealed a strong selection during soybean domestication on Gm SWEET10a. The selection of Gm SWEET10a conferred simultaneous increases in soybean-seed size and oil content as well as a reduction in the protein content. The result was validated using both near-isogenic lines carrying substitution of haplotype chromosomal segments and transgenic soybeans. Moreover,Gm SWEET10b was found to be functionally redundant with its homologue Gm SWEET10a and to be undergoing selection in current breeding, leading the the elite allele Gm SWEET10b, a potential target for present-day soybean breeding. both GmSWEET10a and GmSWEET10b were shown to transport sucrose and hexose, contributing to sugar allocation from seed coat to embryo, which consequently determines oil and protein contents and seed size in soybean. We conclude that past selection of optimal Gm SWEET10a alleles drove the initial domestication of multiple soybean-seed traits and that targeted selection of the elite allele Gm SWEET10b may further improve the yield and seed quality of modern soybean cultivars.

关键词： soybean domestication yield seed quality SWEET