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Activation of mitochondrial ATP-sensitive potassium channels delays ischemia-induced cellular uncoupling in rat heart

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Acta Pharmacologica Sinica 2004年第1期 24-30页

作者： iain c BRUcE china Department of Physiology Faculty of Medicine The University of Hong Kong

AIM: To test the hypothesis that cellular uncoupling induced by myocardial ischemia is mediated by activation of cmitochondrial ATP-sensitive potassium channels (mitoKATP). METHODS: Rat hearts were perfused on a Langendorff apparatus and subjected to 40-min ischemia followed by 30-min reperfusion (I/R). changes in cellular coupling were monitored by measuring whole-tissue resistance. RESULTS: (1) In hearts subjected to I/R, the onset of uncoupling started at (13.3±1.0) mm of ischemia; (2) Ischemic preconditioning (IPc) delayed the onset of uncoupling until (22.7±1.3) min. Blocking mitoKATP channels with 5-

关键词： heart mitochondria potassium channels adenosine ttiphosphate ischemic preconditioning uncoupling

A phylum level analysis reveals lipoprotein biosynthesis to be a fundamental property of bacteria

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Protein & cell 2012年第3期3卷 163-170页

作者： iain c.Sutcliffe Dean J.Harrington Matthew I.Hutchings School of Life Sciences University of Northumbria at NewcastleNewcastle upon TyneNE18STUK Division of Biomedical Science School of Life SciencesUniversity of BradfordWest YorkshireBD71DPUK School of Biological Sciences University of East AngliaNorwich Research ParkNorwichNR47TJUK

Bacterial lipoproteins are proteins that are post-trans-lationally modified with a diacylglyceride at an N-terminal cysteine,which serves to tether these proteins to the outer face of the plasma membrane or to the outer *** paper reviews recent insights into the enzymology of bacterial lipoprotein biosynthe-sis and ***,we use bioinformatic analyses of bacterial lipoprotein signal peptide features and of the key biosynthetic enzymes to consider the distribution of lipoprotein biosynthesis at the phylum level.

关键词： level. outer property

A machine learning approach for regional geochemical data:Platinum-group element geochemistry vs geodynamic settings of the North Atlantic Igneous Province

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Geoscience Frontiers 2021年第3期12卷 42-59页

作者： Jordan J.Lindsay Hannah S.R.Hughes christopher M.Yeomans Jens c.Ø.Andersen iain McDonald camborne School of Mines University of ExeterPenryn CampusPenrynCornwall TR109FEUnited Kingdom School of Earth and Ocean Sciences Main CollegeCardiff UniversityPark PlaceCardiff CF103ATUnited Kingdom

Whilst traditional approaches to geochemistry provide valuable insights into magmatic processes such as melting and element fractionation,by considering entire regional data sets on an objective basis using machine learning algorithms(MLAs),we can highlight new facets within the broader data structure and significantly enhance previous geochemical *** platinum-group element(PGE)budget of lavas in the North Atlantic Igneous Province(NAIP)has been shown to vary systematically according to age,geographic location and geodynamic *** the large multi-element geochemical data set available for the region,MLAs were employed to explore the magmatic controls on these shifting *** key advantage of using machine learning in analysis is its ability to cluster samples across multi-dimensional(i.e.,multi-element)*** NAIP data set is manipulated using Principal component Analysis(PcA)and t-Distributed Stochastic Neighbour Embedding(t-SNE)techniques to increase separability in the data alongside clustering using the k-means *** new multi-element classification is compared to the original geographic classification to assess the performance of both *** workflow provides a means for creating an objective high-dimensional investigation on a geochemical data set and particularly enhances the identification of metallogenic anomalies across the *** techniques used highlight three distinct multi-element end-members which successfully capture the variability of the majority of elements included as input *** end-members are seen to fluctuate in prominence throughout the NAIP,which we propose reflects the changing geodynamic environment and melting ***,the variability of Pt and Pd are not reflected in MLA-based clustering trends,suggesting that they vary independently through controls not readily demonstrated by the NAIP major or trace element data structure(i.e.,other proxies for magmatic differe

关键词： Platinum-group elements Machine learning Plume Geochemistry Metallogenesis Mantle

Reducing the oxidative stress mediates the cardioprotection of bicyclol against ischemia-reperfusion injury in rats

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第6期14卷 487-495页

作者： Jie cUI Zhi LI Ling-bo QIAN Qin GAO Jue WANG Meng XUE Xiao-e LOU iain c.BRUcE Qiang XIA Hui-ping WANG Department of Physiology School of MedicineZhejiang University Department of Physiology Xuzhou Medical College Department of Physiology Zhejiang Medical College

Objective:To investigate the beneficial effect of bicyclol on rat hearts subjected to ischemia-reperfusion(IR) injuries and its possible ***:Male Sprague-Dawley rats were intragastrically administered with bicyclol(25,50 or 100 mg/(kg·d)) for 3 *** IR was produced by occlusion of the coronary artery for 1 h and reperfusion for 3 *** ventricular hemodynamics was continuously *** the end of reperfusion,myocardial infarct was measured by 2,3,5-triphenyltetrazolium chloride(TTc) staining,and serum lactate dehydrogenase(LDH) level and myocardial superoxide dismutase(SOD) activity were determined by *** ventricular myocytes from adult rats were exposed to 60 min anoxia and 30 min reoxygenation to simulate IR *** reperfusion,cell viability was determined with trypan blue;reactive oxygen species(ROS) and mitochondrial membrane potential of the cardiomyocytes were measured with the fluorescent *** mitochondrial permeability transition pore(mPTP) opening induced by ca2+(200 μmol/L) was measured with the absorbance at 520 nm in the isolated myocardial ***:Low dose of bicyclol(25 mg/(kg·d)) had no significant improving effect on all cardiac parameters,whereas pretreatment with high bicyclol markedly reduced the myocardial infarct and improved the left ventricular contractility in the myocardium exposed to IR(Pcyclol(50 mg/(kg·d)) markedly improved the myocardial contractility,left ventricular myocyte viability,and SOD activity,as well decreased infarct size,serum LDH level,ROS production,and mitochondrial membrane potential in rat myocardium exposed to *** reduction of ventricular myocyte viability in IR group was inhibited by pretreatment with 50 and 100 mg/(kg·d) bicyclol(Pca2+ was significantly inhibited by medium ***:Bicyclol exerts cardioprotection against IR injur

关键词： Ischemia-reperfusion injury cardioprotection Oxidative stress Mitochondrial permeability transition pore Bicyclol

A detailed study of metallic glass formation in copper-hafnium-titanium alloys

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Acta Metallurgica Sinica(English Letters) 2012年第6期25卷 409-419页

作者： Ignacio A.Figueroa Sandro Baez-Pimiento John D.Plummer Omar Novelo-Peralta Hywell A.Davies iain Todd Department of Metallic and ceramic Materials Institute for Materials ResearchNational Autonomous University of Mexico(UNAM)Mexico D.F.C.P.04510Mexico Department of Materials Imperial College LondonRoyal School of MinesPrince Consort RoadSW7 2BPU.K. Department of Materials Science and Engineering University of SheffieldMappin StSheffield S1 3JDU.K.

Recently we have reported a number of bulk glass forming compositions in the cuHf-Ti system,with the critical thickness for complete glass formation,dc,ranging from 2 mm to 4 *** order to improve the glass forming ability （GFA） getting even larger dc,the prevailing approach is to use complex multicomponent *** strategy has been investigated by us for the cu-Hf-Ti bulk glass forming alloy cu55Hf25Ti20 using 〉1 *** additions of B,Y,Nb,Ta,Al,Mn,Si or V but with no significant improvement in the ***,it is necessary,in order to utilise the full potential of the base ternary system,to identify the best glass-forming compositions as a basis for extending the search into multi-dimensional compositional ***,cuxHfyTiz alloys,where x=（40-70） ***,y=（5-30） ***,and z=（10-36） ***,were prepared by melt spinning andcopper mould *** composition dependence of the GFA for the cu-Hf-Ti alloys,as measured by dc for rod and ribbon samples,is reported over the composition range given above.

关键词： Metallic glass formation Glassy cu-Hf-Ti alloy Glass formingability Melt spinning copper mould suction-casting

Special issue on new techniques and approaches for neuroscience

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Neuroscience Bulletin 2012年第4期28卷 I0001-I0001页

作者： iain c. Bruce Executive Associate Editor

In this special issue of Neuroscience Bulletin, the topics range over emerging techniques from the nanoscopic to the macroscopic scales. At the nanoscopic level, new ways to obtain images at the individual protein level in synapses take the re- construction of synaptic architecture to a new level, while using chromophores to disable specific proteins at selected subcellular locations allows the assessment of their functional roles in such dynamic processes as growth-cone extension and cell division. The subtle influences of axonal chan- nels and receptors on neurotransmission are revealed by recording from the blebs induced by axotomy, while methods from cloning to chemical computation are being applied to dissecting the structural-functional rules by which ion channels operatet41.

关键词： Special issue on new techniques and approaches for neuroscience

Measurement of copy number variation in single cancer cells using rapid-emulsification digital droplet MDA

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Microsystems & Nanoengineering 2017年第1期3卷 260-266页

作者： Samuel c.Kim Gayatri Premasekharan iain c.clark Hawi B.Gemeda Pamela L.Paris Adam R.Abate Department of Bioengineering and Therapeutic Sciences University of CaliforniaSan Francisco(UCSF)California Institute for Quantitative Biosciences(QB3)San FranciscoSan FranciscoCA 94158USA Department of Urology Division of Hematology&OncologyUniversity of CaliforniaSan Francisco(UCSF)San FranciscoCA 94158USA

Uniform amplification of low-input DNA is important for applications across biology,including single-cell genomics,forensic science,and microbial and viral ***,the requisite biochemical amplification methods are prone to bias,skewing sequence proportions and obscuring signals relating to copy *** droplet multiple displacement amplification enables uniform amplification but requires expert knowledge of microfluidics to generate monodisperse *** addition,existing microfluidic methods are tedious and labor intensive for preparing many ***,we introduce rapid-emulsification multiple displacement amplification,a method to generate monodisperse droplets with a hand-held syringe and hierarchical droplet *** conventional microfluidic devices require >10 min to emulsify a sample,our system requires tens of seconds and yields data of equivalent *** demonstrate the approach by using it to accurately measure copy number variation(cNV)in single cancer cells.

关键词： amplification bias copy number variation ddMDA droplet microfluidics multiple displacement amplification

Ecosystem service delivery in Karst landscapes： anthropogenic perturbation and recovery

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Acta Geochimica 2017年第3期36卷 416-420页

作者： Timothy Quine Dali Guo Sophie M.Green chenglong Tu iain Hartley Xinyu Zhang Jennifer Dungait Xuefa Wen Zhaoliang Song Hongyan Liu Heather Buss Timothy Barrows Richard Evershed Penny Johnes Jeroen Meersmans Geography College of Life and Environmental SciencesUniversity of Exeter Rennes Drive Exeter EX4 4RJ UK Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Beijing 100101 People's Republic of China State Key Laboratory of Environmental Geochemistry Institute of Geochemistry Chinese Academy of Sciences Guiyang 550081 Guizhou Province People's Republic of China Rothamsted Research - North Wyke Okehampton Devon EX20 2SB UK Institute of the Surface-Earth System Science Research Tianjin University Tianjin 300072 People's Republic of China college of Urban and Environmental Sciences Peking University Beijing 100871 People's Republic of China School of Earth Sciences Wills Memorial Building Queens Road Clifton BS8 1RJ UK School of chemistry University of Bristol Cantock's Close Clifton Bristol BS8 1TS UK School of Geographical Sciences University of BristolUniversity Road Clifton Bristol BS8 1SS UK cranfield Soil and Agrifood Institute Cranfield UniversityCranfield Bedfordshire MK43 0AL UK

covering extensive parts of china, Karst landscapes are exceptional because rapid and intensive land use change has caused severe ecosystem degradation within only the last 50 years. The twentieth century intensification in food production through agriculture has led to a rapid deterioration of soil quality, evidenced in reduced crop production and rapid loss of soil. In many areas, a tipping point appears to have been passed as basement rock is exposed and 'rocky desertification' dominates. Through the establishment of the "Soil processes and ecological services in the karst critical zone of SW china"(SPEcTRA)critical Zone Observatory(cZO) we will endevaour to understand the fundmental processes involved in soil production and erosion, and investigate the integrated geophysical-geochemical-ecological responses of the cZ to perturbations. The cZ spans a gradient from undisturbed natural vegetation through human perturbed *** seek to understand the importance of heterogeneity insurface and below-ground morphology and flow pathways in determining the spatial distribution of key stocks(soil,c, vegetation, etc.) and their control on ecosystem service delivery. We will assess the extent to which the highly heterogeneous critical zone resources can be restored to enable sustainable delivery of ecosystem services. This paper presents the cZO design and initial assessment of soil and soil organic carbon stocks and evidence for their stability based on caesium-137(^(137) cs) data.

关键词： Soil degradation Ecosystem services caesium-137 Karst,china

白细胞介素-33促进胶原诱导性关节炎的实验研究

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中华风湿病学杂志 2010年第3期14卷 165-167,217页

作者：穆荣蒋慧蓉李玉宾 iain B McIness 栗占国北京大学人民医院风湿免疫科 100044 英国格拉斯哥大学免疫系

目的探讨新型细胞因子白细胞介素（IL）-33在胶原诱导性关节炎（cIA）发病中的作用。方法建立DBA／1小鼠的cIA模型。以牛II型胶原（cII），完全福氏佐剂（cFA）免疫，从免疫后第21天开始每天注射IL-33或磷酸盐缓冲液（PBS）共5d。免疫后... 详细信息

目的探讨新型细胞因子白细胞介素（IL）-33在胶原诱导性关节炎（cIA）发病中的作用。方法建立DBA／1小鼠的cIA模型。以牛II型胶原（cII），完全福氏佐剂（cFA）免疫，从免疫后第21天开始每天注射IL-33或磷酸盐缓冲液（PBS）共5d。免疫后第28天取小鼠淋巴结细胞体外培养，培养上清的细胞因子浓度以酶联免疫吸附试验（ELISA）法检测或Luminex检测。同时检测血清中抗Ⅱ型胶原抗体和血清中的细胞因子浓度。鼠爪脱钙后行病理检查。采用两样本均数的t检验、r检验进行统计学处理。结果IL-33可使小鼠实验性的关节炎发病加重，致炎性细胞因子和抗Ⅱ型胶原抗体产生增多（P〈0．05），PBS组与IL-33组的IgG2a型Ⅱ型胶原抗体为[（1．24±0．33）mg／L和（1．96±0．16）mg／L，P〈0．05]，而IgGl型抗Ⅱ型胶原抗体为[（1．1±0．4）mg／L和（1．7±0．4）mg／L，P〈0．05]。结论IL-33是炎性关节病中的重要致炎因子。这一IL-1家族新成员是潜在的类风湿关节炎（RA）治疗靶点。

关键词：白细胞介素类关节炎实验胜关节炎类风湿