Activation of mitochondrial ATP-sensitive potassium channels delays ischemia-induced cellular uncoupling in rat heart

作     者：Iain C BRUCE 

作者机构：China Department of Physiology Faculty of Medicine The University of Hong Kong 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2004年第1期

页      面：24-30页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

基　　金：ProjectsupportedbyZhejiangProvincialNaturalScienceFoundationofChinaforTalent(RC99038 398016) theFundforOutstandingYoungScientistsofZhejiangUniversity 

主　　题：heart mitochondria potassium channels adenosine ttiphosphate ischemic preconditioning uncoupling 

摘      要：AIM: To test the hypothesis that cellular uncoupling induced by myocardial ischemia is mediated by activation of cmitochondrial ATP-sensitive potassium channels (mitoKATP). METHODS: Rat hearts were perfused on a Langendorff apparatus and subjected to 40-min ischemia followed by 30-min reperfusion (I/R). Changes in cellular coupling were monitored by measuring whole-tissue resistance. RESULTS: (1) In hearts subjected to I/R, the onset of uncoupling started at (13.3±1.0) mm of ischemia; (2) Ischemic preconditioning (IPC) delayed the onset of uncoupling until (22.7±1.3) min. Blocking mitoKATP channels with 5-