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Down-regulation of eIF5A-2 prevents epithelial-mesenchymal transition in non-small-cell lung cancer cells

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第6期14卷 460-467页

作者： Guo-dong XU Xin-bao shi Le-bo SUN Qing-yun ZHOU Da-wei ZHENG huo-shun shi Yong-liang CHE Zi-shan WANG Guo-feng SHAO Department of Thoracic & Cardiovascular Surgery Lihuili HospitalNingbo Medical CenterAffiliated Hospital of Medical SchoolNingbo University Department of Thoracic & Cardiovascular Surgery Ningbo First HospitalAffiliated Hospital of Medical SchoolNingbo University

Background:Epithelial-mesenchymal transition(EMT) is believed to be the critical process in malignant tumor invasion and metastases,and has a great influence on improving the survival rate in non-small-cell lung cancer(NSCLC) *** studies suggested that eukaryotic initiation factor 5A-2(eIF5A-2) might serve as an adverse prognostic marker of *** detected eIF5A-2 in NSCLC A549 cells,and found that the invasive capability correlates with the eIF5A-2 ***:Transforming growth factor(TGF)-β1 was used to induce EMT in A549 *** blotting,immunofluorescence,wound healing assay,and transwell-matrigel invasion chambers were used to identify phenotype *** blotting was also used to observe changes of the expression of *** down-regulated the eIF5A-2 expression using an eIF5A-2 siRNA and identified the phenotype changes by western blotting and *** tested the change of migration and invasion capabilities of A549 cells by the wound healing assay and transwell-matrigel invasion ***:After stimulating with TGF-β1,almost all A549 cells changed to the mesenchymal phenotype and acquired more migration and invasion *** cells also had higher eIF5A-2 protein ***-regulation of eIF5A-2 expression with eIF5A-2 siRNA transfection could change the cells from mesenchymal to epithelial phenotype and decrease tumor cell migration and invasive capabilities ***:The expression of eIF5A-2 was up-regulated following EMT phenotype changes in A549 cells,which correlated with enhanced tumor invasion and metastatic ***,in the A549 cell line,the process of EMT phenotype change could be reversed by eIF5A-2 siRNA,with a consequent weakening of both invasive and metastatic capabilities.

关键词： Non-small-cell lung cancer(NSCLC) Epithelial-mesenchymal transition(EMT) Eukaryotic initiation factor 5A-2(eIF5A-2) Transforming growth factor(TGF)-β1 A549

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Motion-pressure coupled control and simulation of long-endurance capability for multicapsule stratospheric airships

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Chinese Journal of Aeronautics 2024年第6期37卷 137-150页

作者： Zhiguang shi Wei huo Zongyu ZUO School of Automation Science and Electrical Engineering Beihang UniversityBeijing 100191China Beijing Near Space Airship Technology Development Co. LtdBeijing 100070China

The current study focuses on the motion-pressure coupled control for a multicapsule stratospheric airship and transforms the path-tracking and heading-hold control of airships into guidance tracking with a time-varying weighted sum of longitudinal and lateral velocities by the definition of compound ***,an improved nonlinear predictive control method is provided to reduce the control energy consumption by the rolling optimization of controller parameters based on finite time intervals,ensuring infinite-time path-tracking ***,combined with the proposed cyclic regulation process of safe pressure between internal and external capsules,this study can fully reflect the force-thermal coupled rule of airships under the actions of atmospheric environment and maneuvering force,while evaluating the long-endurance capability of airships under the conditions of safe superheating and *** effectiveness of the motionpressure coupled controller was verified through numerical simulations,which can overcome the influence of environmental wind and achieve a tracking effect for the desired cruise path and compound *** airspeed provided during the cyclic circadian time caused the maximum superheating of the helium controlled within 30*** helium in the internal and external capsules achieved circadian *** equivalent micropore diameter of the capsule of 5 mm can achieve 55 days of long-endurance *** controller satisfies the requirements of cruise-flight application modes for multicapsule stratospheric airships with important engineering value.

关键词： Compound speed Nonlinear predictive control Cyclic pressure regulation Long-endurance capability Stratospheric airship

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Integral precession calibration method of PIGA on linear vibration table

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Chinese Journal of Aeronautics 2024年第3期37卷 219-236页

作者： Chuang SUN shun-qing REN Jun-wei CAO Ru huo Department of Automation Tsinghua UniversityBeijing 100084China School of Astronautics Harbin Institute of TechnologyHarbin 150001China Beijing National Research Center for Information Science and Technology Tsinghua UniversityBeijing 100084China Faculty of Information technology Beijing University of TechnologyBeijing 100124China

Linear vibration table can provide harmonic accelerations to excite the nonlinear error terms of Pendulous Integrating Gyro Accelerometer(PIGA).Integral precession calibration method is proposed to calibrate PIGA on a linear vibration table in this *** on the precise expressions of PIGA’s inputs,the error calibration model of PIGA is *** angular velocity errors of PIGA are suppressed by integer periodic precession and the errors caused by non-integer periods vibrating are *** complete calibration process,including planning,preparation,PIGA testing,and coefficient identification,is designed to optimize the test operations and evaluate the calibration *** effect of the main errors on calibration uncertainty is analyzed and the relative sensitivity function is proposed to further optimize the test *** and simulation results verify that the proposed 10-position calibration method can improve calibration uncertainties after compensating for the related *** order of calibration uncertainties of the second-and third-order coefficients are decreased to 10^(-8)(rad.s^(-1))/g^(2)and 10^(-8)(rad.s^(-1))/g3,*** with the other two classical calibration methods,the calibration uncertainties of PIGA’s nonlinear error coefficients can be effectively reduced and the proportional residual errors are decreased less than 3×10-6(rad.s^(-1))/g by using the proposed calibration method.

关键词： PIGA Linear vibration table Vibration measurement Calibration Error analysis Uncertainty analysis

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Complex Semblance and Its Application

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Journal of Earth Science 2019年第4期30卷 849-852页

作者： Taikun shi Shoudong huo Key Laboratory of Petroleum Resources Research Institute of Geology and Geophysics Chinese Academy of Sciences

Semblance,a measure of multi-trace coherence,has been used extensively in seismic data processing and interpretation such as velocity analysis and fault detection. The traditional algorithm has a difficulty at zero-crossings of seismic recordings. This problem is alleviated by applying a smoothing window at the cost of losing vertical resolutions. In this paper,we improve the algorithm by computing semblance from complex traces. Our initial results show that the complex semblance is smooth at zero-crossings. Because the smoothing time window becomes unnecessary,the higher vertical resolution can be achieved by using small windows or none. Some geological features,like faults and unconformities,appear clearer and easier to identify with the complex semblance. As the advantages are obvious and the implementation is straight-forward with the Hilbert transform,this new algorithm may replace the traditional one in future applications.

关键词： semblance complex trace Hilbert transform velocity analysis fault detection

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The Vignetting Effect of a LAMOST-Type Schmidt Telescope

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Chinese Journal of Astronomy and Astrophysics 2008年第5期8卷 580-590页

作者： Yuan Xue huo-Ming shi National Astronomical Observatories Chinese Academy of Sciences Beijing 100012 China

Optical vignetting of a Schmidt reflector is caused mainly by the spherical primary mirror not being big enough and by the effective light-collecting area of the corrector, which depends on the direction of the star light. Calculations of the vignetting of LAMOST, a special reflecting Schmidt telescope, are made by ray-tracing methods. The results show various features due to LAMOST＇s structure and observing modes. The un-vignetted field is small compared with its field of view. In the outmost portions of the field vignetting in- creases rapidly with the distance to the field center. The vignetting at a general position within the field of view is a function of both its distance to the field center and its position angle. Vignetting varies when the telescope points at different declinations and during observations. There is difference in vignetting between direct imaging and multi-fiber spectroscopy. Vignetting distorts the relative intensity of celestial objects at different positions in a field and also affects accurate sky-estimation and sky-subtraction. The determined vignetting functions may be used to correct the vignetting effect; alternatively flat-fielding may be adopted for the calibration. The effective apertures, which affect the signal-to-noise ratio of the observations, depend on the different declinations the telescope is pointing at and also different positions within a field due to vignetting.

关键词： telescope - methods miscellaneous

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Filters for manufacturability in design optimization of variable stiffness composites

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Chinese Journal of Aeronautics 2021年第4期34卷 153-159页

作者： Ye TIAN Yuhang huo Tielin shi Qi XIA State Key Laboratory of Digital Manufacturing Equipment and Technology Huazhong University of Science and TechnologyWuhan 430074China State Key Laboratory of Structural Analysis for Industrial Equipment Dalian University of TechnologyDalian 116024China

In the design optimization of variable stiffness composites,manufacturing constraints imposed by the automated fiber placement technology must be *** the present paper,two filters are proposed to address this issue,and they are incorporated into the Shepard interpolation-based design optimization framework developed in our previous *** fiber angle arrangement of a composite is represented by a continuous function that interpolates fiber angles at scattered design *** filters are appointed for each design point to deal with two typical manufacturing constraints,i.e.,fiber curvature and gap/*** each design point,the sensitivity is first filtered in a rectangular region around this point,and by this means the fiber curvature is controlled;then in another rectangular region around this design point,the filtered sensitivities are averaged,and the result is used to update the corresponding design *** numerical examples are investigated,and the results show that the proposed method is effective.

关键词： Composite laminates Design optimization Fiber angle Filter Manufacturing constraints

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N-Positive ion activated rapid addition and mitochondrial targeting ratiometric fluorescent probes for in vivo cell H_(2)S imaging

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Frontiers of Chemical Science and Engineering 2022年第1期16卷 64-71页

作者： Yan shi Fangjun huo Yongkang Yue Caixia Yin Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education Institute of Molecular ScienceShanxi UniversityTaiyuan 030006China College of Food Sciences Shanxi Normal UniversityLinfen 041004China Research Institute of Applied Chemistry Shanxi UniversityTaiyuan 030006China

Heterocyclic compound quinoline and its derivatives exist in natural compounds and have a broad spectrum of biological *** play an important role in the design of new structural entities for medical ***,indoles and their derivatives are found widely in *** acids,alkaloids and auxin are all derivatives of indoles,as are dyes,and their condensation with aldehydes makes it easy to construct reaction sites for nucleophilic addition *** this work,we combine these two groups organically to construct a rapid response site(within 30 s)for H_(2)S,and at the same time,a ratiometric fluorescence response is presented throughout the process of H_(2)S *** such,the lower detection limit can reach 55.7 nmol/L for H_(2)*** addition,cell imaging shows that this probe can be used for the mitochondrial targeted detection of endogenous and exogenous H_(2)***,this probe application was verified by imaging H_(2)S in nude mice.

关键词： heterocyclic compound hydrogen sulfide ratiometric mitochondrial targeted

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Investigation on mechanical exhaust of cabin fire in large-space building

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Science China(Technological Sciences) 2008年第1期51卷 65-76页

作者： shi CongLing ZHONG MaoHua huo Ran China Academy of Safety Sciences and Technology Beijing 100029China State Key Laboratory of Fire Science University of Science and Technology of ChinaHefei 230026China

A calculation model for mechanical exhaust rate in large-space building in the case of cabin fire is proposed through theoretical analysis. Full-scale hot smoke tests are then performed to study the cabin fire spreading to large-space building at dif- ferent air change rates (ACH). The result indicates that under the standard pre- scribed ACH, the effective air heights in the large spaces are respectively 6, 4 and 2 m in the case of cabin fires of 0.34, 0.67 and 1 MW. Numerical experiment has been conducted using self-developing two-zone model. The smoke control effi- ciency is compared by varying the large space’s air change rate in the case of cabin fires ranging from 0.25 to 4 MW. The calculation results show that the air change rates are respectively 3, 6, 10 and 10 ACH when the smoke layer is kept above 5 m, indicating that the centralized exhaust rates far exceed the standard prescribed value. To address this problem, a set of subsidiary distributed mechanical exhaust installing in the cabin with high fire loads is proposed. The simulation shows that both from the safety and economy point of view, the adoption of subsidiary dis- tributed cabin exhaust design may effectively reduce the demand of designed air change rate for large-space building.

关键词： large-space cabin mechanical exhaust spill plume transfer lag time

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A discussion of fatigue properties of notched specimens subjected to prestressing treatment machined from FGH4097

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Chinese Journal of Aeronautics 2024年第4期37卷 508-523页

作者： Rongxi shi Dasheng WEI Le HAN shun YANG School of Energy and Power Engineering Beihang UniversityBeijing 100191China Jiangxi Research Institute Beihang UniversityNanchang 330096China Research Institute of Aero-Engine Beihang UniversityBeijing 100191China

Experimental and numerical investigations were performed to reveal the effect of prestress on the fatigue properties of FGH4097 so as to quantitatively evaluate the overspeed benefit of the disc of aeroengine. A corresponding experiment was performed to examine the effect of prestress on the fatigue life of FGH97. A complete model describing the fatigue properties of the prestressed notch specimen was proposed. After the modification of mean stress, stress gradient and prestressing treatment, the model is finally verified by fatigue test of presstressed notch specimens machined from FGH4097 with satisfactory accuracy and good engineering applicability. The new model highlights that for the variable fatigue loads, the introduction of appropriate prestress is expected to benefit to the notch specimens and certainly to the disc of aeroengine regards of their fatigue properties. Both the calculation method of fatigue life parameters and the prestress correction model are proved to have good prediction accuracy.

关键词： Turbine blisk Overspeed test Prestress Fatigue life prediction FGH4097

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Approaches to promoting bone marrow mesenchymal stem cell osteogenesis on orthopedic implant surface

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World Journal of Stem Cells 2020年第7期12卷 545-561页

作者： shi-Cheng huo Bing Yue Department of Bone and Joint Surgery Renji HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China

Bone marrow-derived mesenchymal stem cells(BMSCs)play a critical role in the osseointegration of bone and orthopedic ***,osseointegration between the Ti-based implants and the surrounding bone tissue must be improved due to titanium’s inherent *** modification stands out as a versatile technique to create instructive biomaterials that can actively direct stem cell ***,we summarize the current approaches to promoting BMSC osteogenesis on the surface of titanium and its *** will highlight the utilization of the unique properties of titanium and its alloys in promoting tissue regeneration,and discuss recent advances in understanding their role in regenerative *** aim to provide a systematic and comprehensive review of approaches to promoting BMSC osteogenesis on the orthopedic implant surface.

关键词： Bone marrow mesenchymal stem cells Osseointegration Orthopedic implant Biofunctionalization

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