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玉米乙烯响应因子亚家族的生物信息学鉴定与分析

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济南大学学报(自然科学版) 2025年第1期39卷 87-98页

作者：李焰榕谭孝婷车荣会济南大学生物科学与技术学院山东济南250022

为了全面了解玉米APETALA2/乙烯响应因子(AP2/ERF)超家族中ERF亚家族成员在玉米生长发育和逆境过程中的作用,利用生物信息学手段鉴定和分析玉米Zea mays L.B73基因组ERF亚家族成员。结果表明:共鉴定到87个ERF基因且不均匀地分布在玉米... 详细信息

为了全面了解玉米APETALA2/乙烯响应因子(AP2/ERF)超家族中ERF亚家族成员在玉米生长发育和逆境过程中的作用,利用生物信息学手段鉴定和分析玉米Zea mays L.B73基因组ERF亚家族成员。结果表明:共鉴定到87个ERF基因且不均匀地分布在玉米的10条染色体上,蛋白序列长度为124~603 aa,蛋白质分子质量为13.23~65.44 kDa,理论等电点为4.44~10.84,亚细胞定位于细胞核;进化树分析显示,ERF基因被分为5个组,各组内的基因结构和保守基序的数量、分布等相似,但各组之间存在较大的差异,多数没有内含子,有内含子的基因占比为20.69%;ERF亚家族成员共有10个保守基序,其中前2个保守基序最为保守,且通常以串联方式存在于多数ERF亚家族成员中;启动子分析显示ZmERF基因含有大量光响应、激素响应、胁迫响应和生长发育相关元件,表明玉米ERF亚家族成员在玉米生长发育及非生物胁迫、生物胁迫方面发挥作用。

关键词：生物信息学玉米乙烯响应因子转录因子

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运动休闲特色小镇文体旅融合场景的价值关涉与叙事路径——以莫干山漫运动小镇为中心的考察

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济南大学学报(社会科学版) 2025年第1期35卷 157-168页

作者：车雯张瑞林马培艳济南大学体育学院山东济南250022 北京师范大学体育与运动学院北京100875 福建师范大学体育科学学院福建福州350117

运动休闲特色小镇文体旅融合是新时代推动乡村空间结构重塑和城乡空间秩序重构的重要途径。通过对运动休闲特色小镇文体旅融合场景的价值共创结构与空间生产机理进行剖析,以浙江省德清县莫干山漫运动小镇为案例地,基于场景理论,采用扎... 详细信息

运动休闲特色小镇文体旅融合是新时代推动乡村空间结构重塑和城乡空间秩序重构的重要途径。通过对运动休闲特色小镇文体旅融合场景的价值共创结构与空间生产机理进行剖析,以浙江省德清县莫干山漫运动小镇为案例地,基于场景理论,采用扎根理论,构建出“地方真实表达性”“具身感知性”与“融合生产性”三大主维度的价值关涉。结果显示,运动休闲特色小镇在本土特色性、新奇刺激性与公共利益性方面表现为强表征价值,而知识性、历史性、多元性象征为未来建设重点方向。实现运动休闲特色小镇文体旅融合的价值表达强化路径,可聚焦于打造运动项目文化空间,强化个人情感联结和文化记忆,加强知识植入和理念引领,提升空间文化包容性,创新空间多元符号价值形式,增强空间表征的价值感知。

关键词：运动休闲特色小镇文体旅融合场景价值关涉价值表达

Estimating thermohaline variability of the equatorial Pacific Ocean from satellite altimetry

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Science China Earth Sciences 2016年第11期59卷 2213-2222页

作者： SUN che MA Xiao Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China National Laboratory for Marine Science and Technology Qingdao 266071 China State Key Laboratory of Satellite Ocean Environment Dynamics Second Institute of Oceanography State Oceanic Administration Hangzhou 310012 China

The vertical thermohaline structure in the western equatorial Pacific is examined with a Gravest Empirical Mode(GEM)diagnosis of in-situ mooring measurements. The poor GEM performance in estimating deep thermohaline variability from satellite altimetry confirms a lack of vertical coherence in the equatorial ocean. Mooring observation reveals layered equatorial water with phase difference up to 6 months between thermocline and sub-thermocline variations. The disjointed layers reflect weak geostrophy and resemble pancake structures in non-rotating stratified turbulence. A coherency theorem is then proved, stating that traditional stationary GEM represents in-phase coherent structure and can not describe vertically out-of-phase variability. The fact that stationary GEM holds both spatial and temporal coherence makes it a unique tool to diagnose vertical coherent structure in geophysical flows. The study also develops a non-stationary GEM projection that captures more than 40% of the thermohaline variance in the equatorial deep water.

关键词： Equatorial Pacific TRITON buoys Satellite altimetry GEM Vertical coherence

Enhanced release of the poorly soluble drug itraconazole loaded in ordered mesoporous silica

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Science China chemistry 2015年第3期58卷 400-410页

作者： Xiao Liu Shunai che State Key Laboratory of Metal Matrix Composites School of chemistry and chemical Engineering Shanghai Jiao Tong University

It is known that the energy of the amorphous state of itraconazole loaded in ordered mesoporous materials is high relative to that of the crystalline state and is responsible for enhanced solubility and dissolution rate. We investigated the effects of particle size(0.7–5μm), mesostructure(2D p6 mm, cubic Ia-3d and cubic Fm-3m) and pore size(2.2–15.4 nm) of mesoporous silicas on the release performance of itraconazole. Results indicated that the release performance was not influenced by the particle sizes tested here, that the release performance increased with increasing pore diameter due to the lower probability of drug molecules colliding to recrystallize in large pores, and that the release performance was decreased in the cage-type pore structure(Fm-3m) compared to that in the cylindrical pore structures(p6mm and Ia-3d) because of the small entrance to the cagelike pores that retards the drug release.

关键词： itraconazole poorly soluble enhanced release mesoporous silica

Solid and liquid phase equilibria in mixtures of N, N-dimethylacetamide with chlorinated hydrocarbons

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Chinese Science Bulletin 1997年第11期42卷 918-921页

作者： che, GQ Peng, WL che, B Department of chemistry Zhongshan University Guangzhou China

IN refs.[1,2],we studied the solid and liquid phase equilibria in mixtures of tetra-chloromethane with oxygen and nitrogen-containing compounds,intermolecular interactionswith the formation of solid molecular addition... 详细信息

关键词： phase diagram solid and liquid equilibrium N N-dimethylacetamide.

Precision design of Ti sites for unprecedented catalytic performance

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National Science Review 2024年第7期11卷 67-68页

作者： Haoquan Zheng Shunai che Key Laboratory of Applied Surface and Colloid chemistry Ministry of Education School of Chemistry and Chemical Engineering Shaanxi Normal University School of chemistry and chemical Engineering Shanghai Jiao Tong University

In 1983, Taramasso et al. incorporated titanium(Ti) into porous silica frameworks to endow Ti sites with catalytic oxidation ability [1]. Nowadays, catalytic oxidation reactions using titanium silicate materials have ... 详细信息

关键词： precision design sites catalytic performance unprecedented

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基于装配式建筑的EPC项目成本控制研究

工程建设与设计 2025年第2期 243-245页

作者：车建婷中铁二十二局集团第五工程有限公司重庆400700

针对装配式建筑EPC项目中成本控制的问题,提出了一种限额设计和二级挣值法相结合的成本控制新方法,解决了设计阶段与采购施工阶段成本控制问题,实现了工程的优化。基于蔡家组团L标准分区小学海绵城市建设项目的实例研究表明,该方法不仅... 详细信息

针对装配式建筑EPC项目中成本控制的问题,提出了一种限额设计和二级挣值法相结合的成本控制新方法,解决了设计阶段与采购施工阶段成本控制问题,实现了工程的优化。基于蔡家组团L标准分区小学海绵城市建设项目的实例研究表明,该方法不仅可以有效降低成本,还能保证工程质量。

关键词：装配式建筑 EPC 成本控制采购施工

An altimetric transport index for Kuroshio inflow northeast of Taiwan Island

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Science China Earth Sciences 2015年第5期58卷 697-706页

作者： YAN XiaoMei SUN che Institute of Oceanology Chinese Academy of Sciences

The Kuroshio inflow northeast of Taiwan Island plays an important role in the heat and nutrient balances over the East China Sea(ECS). Based on merged satellite altimeter data and the PCM-1 mooring observation at the East Taiwan Channel(ETC), the study employs a correlation iteration scheme to find the optimal transport index for the Kuroshio inflow. The sea level difference with the highest correlation to the ETC transport is across the ECS shelf break rather than along the PCM-1 line. The counter-intuitive result is caused by large signal noise and poor track coverage of altimeters near the Taiwan coast. The optimal altimetric index is highly correlated with the two-year in-situ measurements as well as the ten-year output of the global assimilation model. It serves as a better estimator of Kuroshio inflow than those using tidal gauge data, and helps pinpoint a 5 cm mismatch of mean sea level in the Keelung tidal record. The mean transport of Kuroshio inflow based on the twenty-year altimetric index is 20.55 Sv with a standard deviation of 3.05 Sv. Wavelet spectrum of the index reveals that semi-annual period dominates the Kuroshio variation northeast of Taiwan Island.

关键词： Kuroshio inflow East Taiwan Channel tidal record in-situ mooring altimeter

Water mass characteristics in the western North Pacific based on a streamfunction projection

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Science China Earth Sciences 2015年第11期58卷 2067-2077页

作者： MA Xiao SUN che Institute of Oceanology Chinese Academy of Sciences College of Earth Sciences University of Chinese Academy of Sciences

A streamfunction projection method called gravest empirical mode(GEM) is applied to the hydrographic section at 137°E to filter out eddy noises in the western North Pacific and derive quantitative ensemble-average water mass properties in the North Equatorial Current region. The GEM fields capture more than 80% of total property variances in the thermocline layer. The core layer structures of key water masses, including the North Pacific Tropical Water(NPTW) and the North Pacific Intermediate Water(NPIW), are examined with a definition of water mass boundary based on property gradient. It shows that a tongue of maximal root-mean-square(RMS) residual exists in the upper half of NPIW for all water properties. These subsurface RMS tongues appear to be close to sharp property gradients. It is the first time a GEM diagnosis is applied to nutrient data, which reveals a drastic difference of N/P reaction rate ratio above and below the maximal-nutrient core at 1250 m. Additionally, a GEM velocity reconstruction successfully produces the North Equatorial Undercurrent(NEUC), demonstrating the stable thermal-wind nature of this newly-discovered current.

关键词： streamfunction projection gravest empirical mode water masses western North Pacific