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Effects of droplet size and spray volume parameters on droplet deposition of wheat herbicide application by using UAV

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International Journal of Agricultural and Biological Engineering 2021年第1期14卷 74-81页

作者： Changfeng Shan Guobin wang Haihong wang Yingjie Xie huizheng wang Shilong wang Shengde Chen Yubin Lan College of Agricultural Engineering and Food Science Shandong University of TechnologyShandong Provincial Engineering Technology Research Center for Agricultural Aviation Intelligent EquipmentZibo 255022ShandongChina Corteva Agroscience Technology(Shanghai)Co. LtdShanghai 201203China Zibo Agricultural and Rural Service Center Zibo 255022ShandongChina National Center for International Collaboration Research on Precision Agricultural Aviation Pesticides Spraying Technology(NPAAC) Ministry of Science and TechnologyCollege of Electronics EngineeringSouth China Agricultural UniversityGuangzhou 510642China Department of Biological and Agricultural Engineering Texas A&M UniversityCollege StationTX 77845USA

With the characteristic of flexible and precise,unmanned aerial vehicles(UAVs)for low volume applications are increasing substantially and quickly around the ***,little attention has been paid to the study of wheat herbicides with UAV,especially the research on the spray volume and droplet size of the herbicide sprayed by *** objectives of this study were to compare the droplet deposition from a typical commercial UAV under four different spray volumes of 7.5 L/hm2,15.0 L/hm2,22.5 L/hm2,and 30.0 L/hm2 and three different volume median diameter(VMD)of 150μm,200μm,and 300μm during winter wheat weeding *** software was used to analyze droplet deposition parameters including the percentage of spray coverage and the number of droplets in various sampling *** test results showed that the droplet deposition waseffected by each factor *** the spray volume was 7.5 L/hm2,the effect of VMD on the percentage of spray coverage was not ***,these variation rules were changed to smaller droplets with greater coverage when the spray volume higher than 15.0 L/*** all treatments,the number of droplets increased with decreasing VMD or increasing spray *** maximum percentage of spray coverage and the number of droplets that were achieved under the VMD of 150μm and the spray volume of 30.0 L/hm2 were 12.8%and 40.0 droplets/cm2,*** variation coefficients of the percentage of spray coverage and the number of droplets were 29.0%-73.3%and 20.2%-54.1%,*** most uniform deposition was achieved under the spray volume of 15.0 L/hm2and the VMD of 150μ*** results revealed the effect of droplet size and spray volume parameters on droplet deposition,which was useful for guiding farmers on how to use UAVs for weeding in winter wheat fields.

关键词： unmanned aerial vehicle spray volume droplet size droplet deposition

辣椒疫霉菌肉碱脂酰转移酶基因CAT的表达、蛋白纯化及结晶

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北方园艺 2020年第11期 42-46页

作者：王会征兰玉彬山东理工大学农业工程与食品科学学院/国家精准农业航空施药技术国际联合研究中心山东理工大学分中心山东淄博255000

肉碱脂酰转移酶CAT是与附着胞的形成紧密相关的一类酶,与辣椒疫霉菌的致病性密切相关。该研究基于辣椒疫霉菌全基因组序列,以辣椒疫霉高致病菌株SD33全基因组DNA为试材扩增CAT基因,采用了原核表达方法诱导表达重组蛋白6×His-CAT,并对... 详细信息

肉碱脂酰转移酶CAT是与附着胞的形成紧密相关的一类酶,与辣椒疫霉菌的致病性密切相关。该研究基于辣椒疫霉菌全基因组序列,以辣椒疫霉高致病菌株SD33全基因组DNA为试材扩增CAT基因,采用了原核表达方法诱导表达重组蛋白6×His-CAT,并对重组蛋白进行亲和层析纯化、分子排阻层析纯化及蛋白结晶试验,以期获得纯蛋白及蛋白晶体。结果表明:经上述方法所获蛋白具有较高的纯度,且有较好的结晶性能,为进一步阐明CAT作用机理奠定基础。

关键词：辣椒疫霉菌肉碱脂酰转移酶表达纯化结晶

基于改进YOLOv7-tiny的番茄叶片病虫害检测方法

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农业工程学报 2024年第10期40卷 194-202页

作者：王会征孙良晨李新龙刘海藤王国宾兰玉彬山东理工大学农业工程与食品科学学院淄博255049

为解决自然环境中番茄叶片病虫害检测场景复杂、检测精度较低,计算复杂度高等问题,该研究提出一种SLPYOLOv7-tiny的深度学习算法。首先,将主干特征提取网络中部分3×3的卷积Conv2D(2D convolution)改为分布偏移卷积DSConv2D(2D Depthwis... 详细信息

为解决自然环境中番茄叶片病虫害检测场景复杂、检测精度较低,计算复杂度高等问题,该研究提出一种SLPYOLOv7-tiny的深度学习算法。首先,将主干特征提取网络中部分3×3的卷积Conv2D(2D convolution)改为分布偏移卷积DSConv2D(2D Depthwise Separable Convolution),以减少网络的计算量,并且使计算速度更快,占用内存更少;其次,将无参数注意力机制(parameter-free attention module,SimAM)融合到骨干特征提取网络中,加强模型对病虫害特征的有效提取能力和特征整合能力;最后,将原始YOLOv7-tiny的CIOU损失函数,更替为Focal-EIOU损失函数,加快模型收敛并降低损失值。试验结果表明,SLP-YOLOv7-tiny模型整体识别精准度、召回率、平均精度均值mAP_(0.5)(IOU阈值为0.5时的平均精度)、mAP_(0.5~0.95)(IOU阈值从0.5到0.95之间的平均精度)分别为95.9%、94.6%、98.0%、91.4%,与改进前YOLOv7-tiny相比,分别提升14.7、29.2、20.2、30个百分点,同时,计算量降低了62.6%。与YOLOv5n、YOLOv5s、YOLOv5m、YOLOv7、YOLOv7-tiny、Faster-RCNN、SSD目标检测模型相比,mAP_(0.5)分别提升了2.0、1.6、2.0、2.2、20.2、6.1和5.3个百分点,而计算量大小仅为YOLOv5s、YOLOv5m、YOLOv7、Faster-RCNN、SSD的31.5%、10.6%、4.9%、4.3%、3.8%。结果表明SLP-YOLOv7-tiny可以准确快速地实现番茄叶片病虫害的检测,且模型较小,可为番茄叶片病虫害的快速精准检测的发展提供一定的技术支持。

关键词：图像处理病虫害目标检测番茄叶片 YOLOv7-tiny 分布偏移卷积注意力机制

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圆锭电磁铸造中电磁场的数值模拟

特种铸造及有色合金 2004年第4期24卷 38-39,41页

作者：郭志芳金俊泽王辉郑贤淑大连理工大学

详细描述了互感耦合模型 ,并且利用此模型计算了电磁铸造中电磁场的分布。结果发现电磁铸造中磁感应强度与输入的电流呈线性变化的关系。利用这一结果提出了一种计算电磁铸造中液柱高度的计算方法 ,并且利用这种方法计算了电流与液柱高... 详细信息

详细描述了互感耦合模型 ,并且利用此模型计算了电磁铸造中电磁场的分布。结果发现电磁铸造中磁感应强度与输入的电流呈线性变化的关系。利用这一结果提出了一种计算电磁铸造中液柱高度的计算方法 ,并且利用这种方法计算了电流与液柱高度的关系 ,计算值与实测值基本符合。

关键词：电磁铸造互感耦合模型电磁场液柱高度

Assessment of In-situ Langley Calibration of Cimel Sunphotometer and the Aerosol Optical Properties at Mt. Waliguan Observatory,China

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Assessment of In-situ Langley Calibration of Cimel Sunphotom...

辣椒疫霉菌果胶裂解酶PL101基因真核表达及致病性分析

第28届中国气象学会年会

作者： huizheng Che,Yaqiang wang,Junying Sun,Xiaochun Zhang Key Laboratory of Atmospheric Chemistry(LAC),Chinese Academy of Meteorological Sciences(CAMS),CMA,Beijing,100081,China

In-situ instrument calibration has been assessed at *** Observatory,China based on continuous CE-318 sunphotometer *** calibration procedure was introduced and calibration results were *** the analysis results,the measurement data obtained during airmass between 2.0 and 6.0 at AM are suitable for Langley method *** final instrument calibration coefficients were achieved based on more than 10 plot curves with the criteria of relative standard deviation（RSD） less than 0.5% for infrared and visible wavelengths and 1.0% for UV wavelengths,*** in-situ calibration coefficients differed less than 0.5% for infrared and visible wavelengths and 1.0% for UV wavelengths from inter-comparison calibration *** on the analysis results,*** Observatory is possible for the calibration of sunphotometer,especially in autumn and winter *** optical properties at the *** Observatory in Northwest China are investigated based on the measurements of aerosol optical depth（AOD） at 500 nm and Angstrom exponent（α） from Septmeber 2009 to August *** values of 0.14±0.07 and 0.59±0.24 are observed for AOD and α,respectively,both of which are characterized by a wide range of values from 0.02 to 1.13 and-0.03 to 1.37,respectively.A seasonal variation is found with high AOD and low α values in spring which could be attributed to the effect of Asian dust events,and low ground AOD level of less than 0.10 in autumn and *** k-means cluster analysis separates the measurements into three aerosol modes based on the AOD and α distributions with the frequency distribution of AOD centering about 0.07±0.002,0.11±0.02,0.18±*** at WLG is significantly larger than those at MLO and IZA because of the contribution of the dust aerosol from desert regions of Western China and Central Asia and the anthropogenic particles from Central China based on the backtrajectory clustering analysis.

关键词： Langley Calibration Aerosol optical depth Angstrom exponent sunphotometer Mt.Waliguan Observatory

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分子植物育种 2021年第22期19卷 7367-7372页

作者：王会征朱俊科兰玉彬山东理工大学农业工程与食品科学学院淄博255000

辣椒疫霉菌(Photophthora capsici)常引起辣椒等多种作物的毁灭性病害,对该病原菌重要功能基因尤其是致病基因的研究具有重要的经济学意义。果胶裂解酶(pectate lyase,PL)为植物病原菌关键致病因子,通过降解寄主细胞壁引起寄主发病。本... 详细信息

辣椒疫霉菌(Photophthora capsici)常引起辣椒等多种作物的毁灭性病害,对该病原菌重要功能基因尤其是致病基因的研究具有重要的经济学意义。果胶裂解酶(pectate lyase,PL)为植物病原菌关键致病因子,通过降解寄主细胞壁引起寄主发病。本研究以辣椒疫霉菌PL关键成员(PL101)为材料,通过真核表达系统进行诱导表达重组蛋白。真核表达载体选用pPIC9K,测序验证后的重组质粒pPIC9K/PL101经电击法转化毕赤酵母GS115表达菌株,对阳性转化子进行甲醇诱导型(Mut^(+))、不同浓度抗生素G418即高拷贝转化子筛选获得表达量可观的重组蛋白表达菌株。重组酵母菌经1%甲醇诱导7 d获得重组蛋白,经硫酸铵沉淀法、Ni-NTA亲和层析纯化后获得纯度较高的PL101纯蛋白。经聚半乳糖醛酸反应法测得的PL101酶活达到970 U/mg,接种健康辣椒叶片测试结果表明PL101具较高致病性,表明PL101是辣椒疫霉菌重要致病因子。本研究为进一步阐明PL作用机理提供科学依据,进而为辣椒的抗病育种提供基因资源。

关键词：辣椒疫霉菌果胶裂解酶真核表达致病性

Aerosol optical properties at *** Observatory,China

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辣椒疫霉菌葡聚糖酶抑制蛋白的原核表达、纯化及结晶

作者： huizheng Che~*,Yaqiang wang,Junying Sun Key Laboratory of Atmospheric Chemistry(LAC),Chinese Academy of Meteorological Sciences(CAMS),CMA,46 Zhong-Guan-Cun S.Ave.,Beijing 100081,China

Aerosol optical properties at the *** Observatory(WLG) in Northwest China are investigated based on the measurements of aerosol optical depth(AOD) at 500 nm and Angstrom exponent(α) from September 2009 to August *** values of 0.14±0.07 and 0.59±0.24 are observed for AOD and Angstrom exponent,respectively,both of which are characterized by a wide range of values from 0.02 to 1.13 and -0.03 to 1.37,respectively.A seasonal variation is found with high AOD and low Angstrom exponent values in spring which could be attributed to the effect of Asian dust events,and low ground AOD level of less than 0.10 in autumn and *** fe-means cluster analysis separates the measurements into three aerosol modes based on the AOD and Angstrom exponent distributions with the frequency distribution of AOD centering about 0.07±0.002,0.11±0.02,and 0.18±*** at WLG is significantly larger than those at Mauna Loa(MLO) and Izana(IZA) because of the contribution of the dust aerosol in spring from desert regions of Western China and fine particles transport from Central China in *** addition,the factors of nearby surface,geographic location and different aerosol components could also contribute to higher AOD at WLG than MLO and IZA,

关键词： Aerosol optical depth Angstrom exponent Sunphotometer ML Waliguan Observatory China

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山东农业科学 2019年第10期51卷 111-116页

作者：王会征兰玉彬山东理工大学农业工程与食品科学学院/国家精准农业航空施药技术国际联合研究中心山东理工大学分中心

葡聚糖酶抑制蛋白(GIP)是辣椒疫霉菌在病理环境下诱导产生的一类蛋白,是病原菌抗性蛋白的一种。本研究根据辣椒疫霉菌全基因组序列,通过生物信息学分析、序列比对获得葡聚糖酶抑制蛋白全长基因序列。以辣椒疫霉高致病菌株SD33全基因组... 详细信息

葡聚糖酶抑制蛋白(GIP)是辣椒疫霉菌在病理环境下诱导产生的一类蛋白,是病原菌抗性蛋白的一种。本研究根据辣椒疫霉菌全基因组序列,通过生物信息学分析、序列比对获得葡聚糖酶抑制蛋白全长基因序列。以辣椒疫霉高致病菌株SD33全基因组为模板扩增GIP基因cDNA,并对其进行相关生物信息学分析。基因经测序验证后与载体pET28a(+)连接,构建好的重组质粒转化大肠杆菌(Escherichia coli Rosetta),用IPTG诱导表达重组蛋白6×His-GIP。重组蛋白在5mol/L尿素变性环境下进行亲和层析纯化,对纯蛋白进行透析、复性、浓缩后进行分子排阻层析纯化,并对获得的纯蛋白进行蛋白结晶试验,为进一步阐明GIP作用机理奠定基础。

关键词：辣椒疫霉菌葡聚糖酶抑制蛋白(GIP) 原核表达纯化结晶

1例百岁老人粗隆间骨折行微创新型仿生髓内钉内固定的围手术期护理

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中西医结合护理 2023年第1期9卷 160-163页

作者：樊玲玲王慧征中国航天科工集团七三一医院骨科北京100074

本文总结1例百岁老人股骨粗隆间骨折行新型仿生髓内钉内固定的围手术期护理经验。采用思维导图宣教模式,给予患者舒适护理干预,开展中医养生护理,采用敲打、按摩的方式提升患者舒适度;制定规范的血栓预防措施,运用加速康复外科理念、个... 详细信息

本文总结1例百岁老人股骨粗隆间骨折行新型仿生髓内钉内固定的围手术期护理经验。采用思维导图宣教模式,给予患者舒适护理干预,开展中医养生护理,采用敲打、按摩的方式提升患者舒适度;制定规范的血栓预防措施,运用加速康复外科理念、个性化功能锻炼路径、递进式康复训练,降低患者围术期深静脉血栓发生风险,促进早期功能恢复。

关键词：股骨粗隆间骨折舒适护理加速康复外科递进式干预延续性护理