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Journal of Genetics and Genomics 2023年第8期50卷 589-599页

作者： Fan Wang Xixi Cai huizhe wei Linghao Zhang Aiwu Dong wei Su State Key Laboratory of Genetic Engineering Collaborative Innovation Center for Genetics and DevelopmentDepartment of BiochemistryInstitute of Plant BiologySchool of Life SciencesFudan UniversityShanghai 200438China

Cytokinins influence many aspects of plant growth and *** cytokinin biosynthesis and signaling have been well studied in planta,little is known about the regulatory effects of epigenetic modifications on the cytokinin ***,we reveal that mutations to Morf Related Gene(MRG)proteins MRG1/MRG2,which are readers of trimethylated histone H3 lysine 4 and lysine 36(H3K4me3 and H3K36me3),result in cytokinin hyposensitivity during various developmental processes,including callus induction and root and seedling growth *** to the mrg1 mrg2 mutant,plants with a defective AtTCP14,which belongs to the TEOSINTE BRANCHED,CYCLOIDEA,AND PROLIFERATING CELL FACTOR(TCP)transcription factor family,are insensitive to ***,the transcription of several genes related to cytokinin signaling pathway is ***,the expression of Arabidopsis thaliana HISTIDINE-CONTAINING PHOSPHOTRANSMITTER PROTEIN 2(AHP2)decreases significantly in the mrg1 mrg2 and tcp14-2 *** also confirm the interaction between MRG2 and TCP14 in vitro and in ***,MRG2 and TCP14 can be recruited to AHP2 after recognizing H3K4me3/H3K36me3 markers and promote the histone-4 lysine-5 acetylation to further enhance AHP2 *** summary,our research elucidate a previously unknown mechanism mediating the effects of MRG proteins on the magnitude of the cytokinin response.

关键词： Arabidopsis Cytokinin response Histone methylation reader TCP transcription factors Transcription regulation

水稻成花素基因Hd3a在ABA调控种子萌发中的功能研究

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复旦学报（自然科学版） 2023年第2期62卷 189-197页

作者：魏会哲李成章王帆张凌豪苏伟复旦大学生命科学学院植物科学研究所上海200438 复旦大学遗传工程国家重点实验室上海200438

Hd3a(Heading date 3a)是拟南芥成花素基因FT(Flowering Locus T)的同源基因,作为水稻开花的强诱导因子,对短日照条件下水稻的开花具有重要的促进作用。Hd3a在水稻发育中是否具有其他功能,目前的认识仍非常有限。在我们的研究中,通过对H... 详细信息

Hd3a(Heading date 3a)是拟南芥成花素基因FT(Flowering Locus T)的同源基因,作为水稻开花的强诱导因子,对短日照条件下水稻的开花具有重要的促进作用。Hd3a在水稻发育中是否具有其他功能,目前的认识仍非常有限。在我们的研究中,通过对Hd3a基因组序列进行生物信息学分析,发现Hd3a的启动子序列包含响应脱落酸(Abscisic Acid,ABA)信号的ABRE(ABA-Responsive Element)元件。荧光素酶发光反应和RT-qPCR分析都证实了Hd3a的转录表达随外源ABA增加而减少。通过不同浓度的ABA处理水稻野生型和hd3a缺失突变体种子,结果表明hd3a缺失突变体种子萌发明显被抑制,暗示了Hd3a缺失导致种子萌发中对ABA敏感性增强,同时hd3a突变体中ABA信号途径响应基因ABI5(ABA Insensitive 5)、ABL1(ABI5-Like1)的表达量升高,表明Hd3a可负调控ABA信号。综上所述,Hd3a可以响应ABA信号调控水稻种子萌发,这些研究结果对于深入理解水稻Hd3a基因的生物学功能具有重要意义。

关键词：水稻脱落酸成花素种子萌发

河北省高校英语专业课程设置合理性的研究与思考

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河北经贸大学学报（综合版） 2012年第3期12卷 121-125页

作者：任强魏慧哲王琪河北师范大学外国语学院河北石家庄050024

新世纪给我国英语专业本科教育带来的不仅是机遇还有挑战。河北省内高校的英语专业课程设置大多结构狭窄,内容保守过时,远远不能满足社会发展对英语人才的需要。未来的改革可从以下几方面着手:适当改革相关专业知识课程;注重学生的专业... 详细信息

新世纪给我国英语专业本科教育带来的不仅是机遇还有挑战。河北省内高校的英语专业课程设置大多结构狭窄,内容保守过时,远远不能满足社会发展对英语人才的需要。未来的改革可从以下几方面着手:适当改革相关专业知识课程;注重学生的专业实习工作;建立本科生导师制等。

关键词：英语专业课程设置课程改革

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企业社会责任浅析

价值工程 2006年第5期25卷 89-91页

作者：魏慧哲都继萌河北经贸大学石家庄050061

随着经济与社会的不断发展,企业社会责任越来越为人们关注。本文对企业社会责任的内涵、作用进行了分析,在此基础上分析了我国企业社会责任推行现状并提出了相应的改进策略。

关键词：企业社会责任非政府组织

淇河鲫R-spondin1基因cDNA克隆和表达

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水产学报 2019年第4期43卷 731-741页

作者：吴利敏李永婧桑甜魏会哲段胜华李牧童王磊马晓田雪董传举刘慧芬李学军河南师范大学水产学院

为探索R-spondin1(Rspo1)基因在雌核发育三倍体鱼淇河鲫性别决定与分化过程中的作用,本研究利用RACE技术克隆Rspo1基因cDNA序列,同时分析它的时空表达模式,并检测芳香化酶抑制剂Letrozole及高温养殖诱导性逆转下它在性腺中的表达情况。... 详细信息

为探索R-spondin1(Rspo1)基因在雌核发育三倍体鱼淇河鲫性别决定与分化过程中的作用,本研究利用RACE技术克隆Rspo1基因cDNA序列,同时分析它的时空表达模式,并检测芳香化酶抑制剂Letrozole及高温养殖诱导性逆转下它在性腺中的表达情况。将扩增的PCR产物经亚克隆测序、拼接后获得淇河鲫Rspo1 cDNA序列,长1 243 bp(MH243761),包含5′非编码区318 bp,3′非编码区127 bp和开放读码框798 bp,共编码265个氨基酸残基。氨基酸序列比对及系统进化分析结果显示,淇河鲫Rspo1与其他鲤科鱼类同源性较高,与哺乳类、爬行类等脊椎动物同源性相对较低;组织分布检测结果显示,Rspo1基因在淇河鲫各个组织中均有表达,其中肌肉组织表达量最高,卵巢次之,而在脾脏、肾脏、心脏组织中表达量较低;在胚胎发育过程中Rspo1的表达量在未受精卵与受精卵中无差异,随着胚胎发育而降低,神经胚期最低,从尾芽期至出膜期又逐步升高。胚后阶段,Rspo1在性腺中的表达量从淇河鲫性别决定与分化关键时期(孵化后20 d)开始上调。Letrozole处理或高温养殖引起的性逆转中,Rspo1在性腺中表达量升高。研究表明,淇河鲫Rspo1作为一个母源性因子可能在卵巢分化与维持中起到一定作用,同时它可能也参与精子发生过程。

关键词：淇河鲫 Rspo1 克隆卵巢分化性逆转

Optimization Model of Oil-Volume Marking with Tilted Oil Tank

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Open Journal of Optimization 2012年第2期1卷 20-24页

作者： wei Xie Xiaojing Wang huizhe Cui Jun Chen Department of Mathematics Sichuan University of Science & Engineering Zigong China School of Automation and Electronic Information Sichuan University of Science & Engineering Zigong China School of Computer Science Sichuan University of Science & Engineering Zigong China

In this paper, the relationship model between the oil volume and the vertically tilting parameter (α), the horizontally tilting parameter (β) and the displayed height of oil (h*) is first constructed with the tilted oil tank. Then, based on the data of the oil output volume at different time of day, an optimization model of oil-volume marking with tilted oil tank is established. Finally, parameters α = 2.2° and β = 3.05° are estimated by using nonlinear least squares method and the marking number of the tank-volume meter is given.

关键词： Optimization Model Oil Tank Tilt Oil-Volume Marking Problem Least Squares Method Parameter Estimation

Peripheral nerve allograft prepared by an acellular,hypotonic tissue engineering method

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Neural Regeneration Research 2010年第8期5卷 630-634页

作者： huizhe Sun Wenying Dai Jie Wang Xiaojie Tong wei Tian Jun Xu Department of Human Anatomy Shenyang Medical College Shenyang 110034 Liaoning Province China Central Research Institute Shenyang Infectious Disease Hospital Shenyang 110006 Liaoning Province China Department of Biochemistry and Molecular Biology Shenyang Medical College Shenyang 110034 Liaoning Province China Department of Human Anatomy China Medical University Shenyang 110001 Liaoning Province China Central Laboratory of Shenyang Medical College Shenyang 110034 Liaoning Province China

BACKGROUND：Tissue engineered acellular nerves are good autologous nerve substitutes. Acellular peripheral nerves prepared using a conventional chemical extraction method cause a great deal of damage to nerve structures, and the allograff affects the nerve regeneration following ***：To prepare peripheral nerve grafts through an acellular tissue engineering method, and observe their histology, ultrastructure, protein components and ***, TIME AND SETTING：A randomized, controlled, in vivo nerve tissue engineering experiment was performed at the Department of Biochemistry and Molecular Biology, Shenyang Medical College, China, from September 2006 to June ***：Triton X-100, Pepstatin A, Aprotinin and Leupeptin were purchased from Sigma, USA; Tris （hydroxymethyl） aminomethane was purchased from Gibco, ***：The bilateral sciatic nerves of Wistar rats were harvested, treated with 0.05 mol/L Tris-HCI buffer, followed by proteinase inhibitor and Triton X-100 to prepare acellular peripheral nerves. The nerves were implanted in the quadriceps femoris muscle of healthy Wistar *** OUTCOME MEASURES：Tissue structure and ultrastructure of acellular peripheral nerves were observed by optical microscopy and scanning electron microscopy. Growth associated proteins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Nerve allograft and the surrounding muscles were observed by hematoxylin-eosin ***：Acellular treatment eliminated Schwann cells, epineurium or perineurium cells, myelin sheaths and axons of nerve fibers in normal peripheral nerves, while the spatial structure, comprising basement membrane tubes of Schwann cells and the extracellular matrix of perineurium and nerve fascicles was maintained. Protein bands at the region of 30 kD were no longer visible, had slightly decreased at 43 kD and remained unchanged at 65 kD. Following implantation for 7 days, epineurium cells were abso

关键词： nerve peripheral nerve tissue engineering extracellular matrix regeneration allografts rats

EST-SSR Marker Linked to Cucumber Target Leaf Spot Resistance-related Gene

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EST-SSR Marker Linked to Cucumber Target Leaf Spot Resistanc...

基于星点图椭圆度分布的光学系统装配误差计算方法

作者： huizhe Wang,Shuju Li,Ruihuan Yang,wei Guan

Cucumber target leaf spot(Corynespora cassiicola(Berk.& Curt.) wei.),a worldwide fungicidal disease of cucumber,was first reported in Europe in *** becomes a serious problem in cucumber protected cultivation in northern part of China recent years.A 241 F population derived from F of Q5(resistant female) and P57-1(susceptible male) was used as materials,their resistances to Corynespora cassiicola of cucumber were identified through seedling *** results indicated that the resistance to Corynespora cassiicola was controlled by a pair of recessive gene,and the susceptible was in the mode of incomplete dominant *** polymorphism between resistant and susceptible bulk of cucumber target leaf spot were studied using BSA method and SSR,EST-SSR,ISSR technologies.112 SSR,29 EST-SSR and 100 ISSR markers were screened,and a co-dominant EST-SSR marker was *** resistant and susceptible F plants possessed the 203 bp and the 207 bp fragments,respectively,and the mid-plants possessed both two *** EST-SSR marker was closely linked to the cucumber target leaf spot resistance-related gene,and the genetic distance between the marker and the gene was 2.9 *** difference of the two fragments lied in the insert or deletion of 4 *** of 47 inbred lines through seedling inoculation were compared with their results of marker detection,the coincidence rate was 91.5%.

关键词： Cucumber Corynespora cassiicola resistance-related gene EST-SSR

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红外与激光工程 2022年第5期51卷 307-315页

作者：吴伟罗自荣杨慧哲曹玉君尚建忠国防科技大学智能科学学院湖南长沙410073 国防科技大学前沿交叉学科学院湖南长沙410073 湖南交通工程学院高科技研究院湖南衡阳421001

计算机辅助装调技术的出现大大降低了光学系统装调的难度,但是目前提出的大部分装配误差计算方法都基于波像差系数,在应用过程中还需要额外的波前传感器。基于星点图在不同视场中的椭圆度分布,提出了一种不依赖于波前传感器的装配误差... 详细信息

计算机辅助装调技术的出现大大降低了光学系统装调的难度,但是目前提出的大部分装配误差计算方法都基于波像差系数,在应用过程中还需要额外的波前传感器。基于星点图在不同视场中的椭圆度分布,提出了一种不依赖于波前传感器的装配误差计算方法,该方法只需要CCD或CMOS等图像传感器即可实现光学系统的装配误差计算。基于矢量像差理论推导了该方法的理论基础,采用椭圆度参数量化了装配误差对星点图的影响规律,揭示了椭圆度分布与装配误差之间的非线性函数关系,在此基础上,以多视场下的星点图椭圆度分布为优化目标,将光学系统的装配误差求解问题转化成多目标优化问题,可通过智能优化算法进行求解。以Hilbert两反式光学系统为例,基于三个视场的椭圆度分布计算次镜的四个侧向装配误差,仿真结果表明该方法的计算精度可达微米级,满足实际装调需求,验证了该方法的正确性,对促进计算机辅助装调技术的工程化应用具有重要意义。

关键词：计算机辅助装调装配误差计算星点图椭圆度

事故案例展示方式和发展阶段对认知的影响研究

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安全与环境学报 2023年第11期23卷 4082-4088页

作者：丁慧哲侯玮秦洪利魏利娜河北工程大学矿业与测绘工程学院河北邯郸056038 河南理工大学安全科学与工程学院河南焦作454000

为研究安全培训中事故案例展示方式和发展阶段对认知的影响,以皮电和眼动追踪生理测量指标反映内在认知,设计双因素重复测量方差试验进行研究。针对事故原因设计调查问卷以反映培训效果。试验选择70位学生分成两组作为被试,统计分析皮... 详细信息

为研究安全培训中事故案例展示方式和发展阶段对认知的影响,以皮电和眼动追踪生理测量指标反映内在认知,设计双因素重复测量方差试验进行研究。针对事故原因设计调查问卷以反映培训效果。试验选择70位学生分成两组作为被试,统计分析皮电和眼动追踪4个生理指标的差异性和调查问卷数据。结果表明:视频相对于文字,情境诱发性更好;被试对事故前兆线索的关注度、警觉性更好;事前和事后阶段杂乱眼动行为偏少。对不同危险线索的注视时间显著不同反映出被试先验知识影响对事故前兆的感知,而此过程在文字下表现不明显。此外,平均注视时间反映视频下全程认知难度高于文字;无论哪种方式危险呈现时均以专注的注视行为为主;对事后原因分析关注度均不高,这与调查问卷对比分析结果一致。因此,事故案例培训过程可优先以视频动态方式展示,但培训教师需明确事故关键线索,特别强调事故原因以提高培训效果。

关键词：安全社会科学安全培训事故案例生理测量统计分析

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