Histone methylation readers MRG1/MRG2 interact with the transcription factor TCP14 to positively modulate cytokinin sensitivity in Arabidopsis

作     者：Fan Wang Xixi Cai Huizhe Wei Linghao Zhang Aiwu Dong Wei Su Fan Wang;Xixi Cai;Huizhe Wei;Linghao Zhang;Aiwu Dong;Wei Su

作者机构：State Key Laboratory of Genetic EngineeringCollaborative Innovation Center for Genetics and DevelopmentDepartment of BiochemistryInstitute of Plant BiologySchool of Life SciencesFudan UniversityShanghai 200438China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2023年第50卷第8期

页      面：589-599页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：supported by grants from the National Natural Science Foundation of China(31970530 31930017 and 31671263) 

主　　题：Arabidopsis Cytokinin response Histone methylation reader TCP transcription factors Transcription regulation 

摘      要：Cytokinins influence many aspects of plant growth and *** cytokinin biosynthesis and signaling have been well studied in planta,little is known about the regulatory effects of epigenetic modifications on the cytokinin ***,we reveal that mutations to Morf Related Gene(MRG)proteins MRG1/MRG2,which are readers of trimethylated histone H3 lysine 4 and lysine 36(H3K4me3 and H3K36me3),result in cytokinin hyposensitivity during various developmental processes,including callus induction and root and seedling growth *** to the mrg1 mrg2 mutant,plants with a defective AtTCP14,which belongs to the TEOSINTE BRANCHED,CYCLOIDEA,AND PROLIFERATING CELL FACTOR(TCP)transcription factor family,are insensitive to ***,the transcription of several genes related to cytokinin signaling pathway is ***,the expression of Arabidopsis thaliana HISTIDINE-CONTAINING PHOSPHOTRANSMITTER PROTEIN 2(AHP2)decreases significantly in the mrg1 mrg2 and tcp14-2 *** also confirm the interaction between MRG2 and TCP14 in vitro and in ***,MRG2 and TCP14 can be recruited to AHP2 after recognizing H3K4me3/H3K36me3 markers and promote the histone-4 lysine-5 acetylation to further enhance AHP2 *** summary,our research elucidate a previously unknown mechanism mediating the effects of MRG proteins on the magnitude of the cytokinin response.