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Genome-wide identification of the CONSTANS-LIKE(COL)family and mechanism of fruit senescence regulation by PpCOL8 in sand pear(Pyrus pyrifolia)

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Journal of Integrative Agriculture 2024年第4期23卷 1222-1237页

作者： Yue Xu Shurui Song huiying wang Xilong Cao Xinran Zhao Wenli wang Liyue Huo Yawei Li Misganaw Wassie Bin Lu Liang Chen Haiyan Shi College of Horticulture Hebei Agricultural UniversityBaoding 071001China Wuhan Botanical Garden Chinese Academy of SciencesWuhan 430074China

Pyrus pyrifolia Nakai‘Whangkeumbae'is a sand pear fruit with excellent nutritional quality and taste.However,the industrial development of pear fruit is significantly limited by its short shelf life.Salicylic acid(SA),a well-known phytohormone,can delay fruit senescence and improve shelf life.However,the mechanism by which SA regulates CONSTANS-LIKE genes(COLs)during fruit senescence and the role of COL genes in mediating fruit senescence in sand pear are poorly understood.In this study,22 COL genes were identified in sand pear,including four COLs(Pp COL8,Pp COL9a,Pp COL9b,and Pp COL14)identified via transcriptome analysis and 18 COLs through genome-wide analysis.These COL genes were divided into three subgroups according to the structural domains of the COL protein.Pp COL8,with two B-box motifs and one CCT domain,belonged to the first subgroup.In contrast,the other three Pp COLs,Pp COL9a,Pp COL9b,and Pp COL14,with similar conserved protein domains and gene structures,were assigned to the third subgroup.The four COLs showed different expression patterns in pear tissues and were preferentially expressed at the early stage of fruit development.Moreover,the expression of Pp COL8 was inhibited by exogenous SA treatment,while SA up-regulated the expression of Pp COL9a and Pp COL9b.Interestingly,Pp COL8 interacts with Pp MADS,a MADS-box protein preferentially expressed in fruit,and SA up-regulated its expression.While the production of ethylene and the content of malondialdehyde(MDA)were increased in Pp COL8-overexpression sand pear fruit,the antioxidant enzyme(POD and SOD)activity and the expression of Pp POD1 and Pp SOD1 in the sand pear fruits were down-regulated,which showed that Pp COL8 promoted sand pear fruit senescence.In contrast,the corresponding changes were the opposite in Pp MADS-overexpression sand pear fruits,suggesting that Pp MADS delayed sand pear fruit senescence.The co-transformation of Pp COL8 and Pp MADS also delayed sand pear fruit senescence.The results of this study revealed that Pp COL8 can pl

关键词： Pyrus pyrifolia CONSTANS-LIKE gene salicylic acid fruit senescence MADS

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Copper-induced formation of heterostructured Co_(3)O_(4)/CuO hollow nanospheres towards greatly enhanced lithium storage performance

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Chinese Chemical Letters 2024年第2期35卷 594-598页

作者： Junjun Zhang huiying Lu Tianhao Yao Xin Ji Qingmiao Zhang Lingjie Meng Jianmin Feng Hongkang wang College of Geography & Environment Xianyang Normal UniversityXianyang 712000China State Key Lab of Electrical Insulation and Power Equipment Center of Nanomaterials for Renewable Energy (CNRE)School of Electrical EngineeringXi'an Jiaotong UniversityXi'an 710049China School of Chemistry Xi'an Key Laboratory of Sustainable Energy Material Chemistryand Instrumental Analysis CenterXi'an Jiaotong UniversityXi'an 710049China

We report a facile template-free fabrication of heterostructured Co_(3)O_(4)/CuO hollow nanospheres using pre-synthesized Co/Cu-glycerate as conformal precursor.The introduction of copper nitrate in the solvothermal reaction system of glycerol/isopropanol/cobalt nitrate readily induces the conversion from solid Co-glycerate to hollow Co/Cu-glycerate nanospheres,and the effect of the Co/Cu atomic ratio on the structure evolution of the metal glycerates as well as their corresponding oxides were investigated.When examined as anode materials for lithium-ion batteries,the well-defined Co_(3)O_(4)/CuO hollow nanospheres with Co/Cu molar ratio of 2.0 demonstrate excellent lithium storage performance,delivering a high reversible capacity of 930 mAh/g after 300 cycles at a current density of 0.5 A/g and a stable capacity of 650 mAh/g after 500 cycles even at a higher current density of 2.0 A/g,which are much better than their counterparts of bare CuO and Co_(3)O_(4).The enhanced lithium storage performance can be attributed to the synergistic effect of the CuO and Co_(3)O_(4)heterostructure with hollow spherical morphology,which greatly enhances the charge/electrolyte transfer and effectively buffers the volume changes upon lithiation/delithiation cycling.

关键词： Lithium-ion batteries Co/Cu-glycerate Hollow nanospheres Co_(3)O_(4)/CuO heterostructure Electrochemical properties

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Functional annotation map of natural compounds in traditional Chinese medicines library: TCMs with myocardial protection as a case

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药学学报:英文版 2023年第9期13卷 3802-3816页

作者： Xudong Xing Mengru Sun Zifan Guo Yongjuan Zhao Yuru Cai Ping Zhou huiying wang Wen Gao Ping Li Hua Yang State Key Laboratory of Natural Medicines School of Traditional Chinese PharmacyChina Pharmaceutical UniversityNanjing 211198China

The chemical complexity of traditional Chinese medicines(TCMs) makes the active and functional annotation of natural compounds challenging. Herein, we developed the TCMs-Compounds Functional Annotation platform(TCMs-CFA) for large-scale predicting active compounds with potential mechanisms from TCM complex system, without isolating and activity testing every single compound one by one. The platform was established based on the integration of TCMs knowledge base, chemome profiling, and high-content imaging. It mainly included:(1) selection of herbal drugs of target based on TCMs knowledge base;(2) chemome profiling of TCMs extract library by LC-MS;(3) cytological profiling of TCMs extract library by high-content cell-based imaging;(4) active compounds discovery by combining each mass signal and multi-parametric cell phenotypes;(5) construction of functional annotation map for predicting the potential mechanisms of lead compounds. In this stud TCMs with myocardial protection were applied as a case study, and validated for the feasibility and utility of the platform. Seven frequently used herbal drugs(Ginseng, etc.) were screened from 100,000 TCMs formulas for myocardial protection and subsequently prepared as a library of 700 extracts. By using TCMs-CFA platform, 81 lead compounds, including 10 novel bioactive ones, were quickly identified by correlating 8089mass signals with 170,100 cytological parameters from an extract library. The TCMs-CFA platform described a new evidence-led tool for the rapid discovery process by data mining strategies, which is valuable for novel lead compounds from TCMs. All computations are done through Python and are publicly available on GitHub.

关键词： Knowledge discovery Metabolomics High content screening Cell phenotype Ginseng Ginsenosides

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Iron adsorption engineering facilitated by Cu doping on cobalt hydroxide host with enhanced oxygen evolution reaction

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Nano Research 2023年第2期16卷 2111-2118页

作者： Xinyu Zhang Yiwen Dong huiying wang Ziyi Zhao Wenchun Jiang Bin Dong Han Hu Chenguang Liu Yongming Chai State Key Laboratory of Heavy Oil Processing College of Chemistry and Chemical EngineeringChina University of Petroleum(East China)Qingdao 266580China College of New Energy China University of Petroleum(East China)Qingdao 266580China

Iron plays a crucial role in improving the oxygen evolution reaction(OER)activity of hydroxide materials.Increasing the number of iron active sites at the solid–liquid interface is beneficial to enhancing the OER performance of catalysts but still challenging.Here,by systematic exploring the activity trends of M(OH)_(x)and Cu-M(OH)_(x)(M=Mn,Cu,Ni,Fe,and Co),we discover that the Cu doping can promote the deposition of Fe active sites on metal hydroxide and Cu-Co(OH)2 shows the most favorable iron adsorption capacity.When loaded on a conductive substrate(cobalt foam(CF),the M-Cu-Co(OH)2/CF(Co(OH)_(2))prepared by molten salt method)exhibits an attractive low overpotential of 337 mV at 1,000 mA·cm^(−2).Using in anion exchange membrane(AEM)water electrolyzer,the single cell with M-Cu-Co(OH)_(2)/CF as anode catalyst performs a stable cell voltage of 2.02 V to reach 1,000 mA·cm^(−2)over 24 h,indicating a great application potential for actual electrolytic water.Therefore,the promoted adsorption of copper on iron provides a new perspective for further enhancing the OER activity of other metal hydroxides.

关键词： copper doping promoted Fe adsorption industrial-level current density anion exchange membrane water electrolyzer oxygen evolution reaction

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New Quality Productive Forces Bring Qualitative Leap in Industrial Development

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中国新闻发布(英文版) 2024年第5期 11-16页

作者： Zhao huiying

The concept of new quality productive forces was first proposed by Chinese President Xi Jinping during his September 2023 inspection tour of Heilongjiang,China's northeasternmost province.In December 2023,the central economic work conference once again stressed the necessity of accelerating the formation of new quality productive forces.

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Identification,characterization,and verification of miR399 target gene in grape

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Horticultural Plant Journal 2024年第1期10卷 91-102页

作者： Maosong Pei Hainan Liu Tonglu Wei huiying Jin Yihe Yu Mengting Ma Xiaomeng Song Rundong Dai Dalong Guo Henan Engineering Technology Research Center of Quality Regulation and Controlling of Horticultural Plants College of Horticulture and Plant ProtectionHenan University of Science and TechnologyLuoyangHenan 471023China

The microRNA miR399 plays an important role in phosphorus signal transduction pathways in plants.Previously,miR399 was shown to be closely associated with berry ripening in grape(Vitis vinifera).The objective of the present study was to elucidate the evolutionary characteristics of the miR399 gene family in grape and to verify the cleavage effect on the target genes.Grape miR399s were identified by miRNA sequencing and retrieval from the miRBase database.The mature sequences and precursor sequences were subjected to phylogenetic analysis to reconstruct evolutionary trees,as well as secondary structure analysis of the precursor sequence,and prediction of target genes.The cisacting elements in the miR399 promoter were predicted and the cleavage effect of grape miR399b on its target genes was verified.The grape miR399 family comprised nine precursor sequences and nine mature sequences.The precursor sequences formed a typical and stable stem—loop structure.The minimum folding free energy ranged from-55.70 kcal·mol^(-1)to-37.40 kcal·mol^(-1).Multiple sequence alignment revealed that the miR399 family was highly conserved.The grape miR399 family was phylogenetically closely related to peach,apple,and citrus miR399s.Grape miR399s were predicted to target inorganic phosphate transporter 1—3,phospholipase D delta-like,and beta-glucuronosyltransferase.The cleavage effect of grape miR399b on the target genes was verified by means of a dual-luciferase assay and 5’RLM-RACE.Histochemical GUS staining showed that the promoter activity of miR399b was promoted by GA3treatment.

关键词： Grape miR399 Phosphorus metabolism Early ripening

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Control of defensive behavior by the nucleus of Darkschewitsch GABAergic neurons

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National Science Review 2024年第4期11卷 70-83页

作者： huiying Zhao Jinrong Liu Yujin Shao Xiang Feng Binhan Zhao Li Sun Yijun Liu Linghui Zeng Xiao-ming Li Hongbin Yang Shumin Duan Yan-qin Yu Department of Neurology of Second Affiliated Hospital and School of Brain Science and Brain Medicine Zhejiang University School of Medicine Nanhu Brain-Computer Interface Institute Liangzhu Laboratory MOE Frontier Science Center for Brain Science and Brain-Machine Integration State Key Laboratory of Brain-Machine Intelligence Zhejiang University NHC and CAMS Key Laboratory of Medical Neurobiology Zhejiang University Key Laboratory of Novel Targets and Drug Study for Neural Repair of Zhejiang Province School of Medicine Hangzhou City University

The nucleus of Darkschewitsch(ND), mainly composed of GABAergic neurons, is widely recognized as a component of the eye-movement controlling system. However, the functional contribution of ND GABAergic neurons(NDGABA) in animal behavior is largely unknown. Here, we show that NDGABA neurons were selectively activated by different types of fear stimuli, such as predator odor and foot shock.Optogenetic and chemogenetic manipulations revealed that NDGABA neurons mediate freezing behavior.Moreover, using circuit-based optogenetic and neuroanatomical tracing methods, we identified an excitatory pathway from the lateral periaqueductal gray(l PAG) to the ND that induces freezing by exciting ND inhibitory outputs to the motor-related gigantocellular reticular nucleus, ventral part(Gi V). Together,these findings indicate the NDGABA population as a novel hub for controlling defensive response by relaying fearful information from the l PAG to Gi V, a mechanism critical for understanding how the freezing behavior is encoded in the mammalian brain.

关键词： nucleus of Darkschewitsch GABAergic population defensive behavior freezing neural circuitry

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National Cultural Parks Unveiled to Explore Ancient Chinese Civilization

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China News Release 2024年第1期 28-31页

作者： Zhao huiying 不详

The over-500-kilometer-long Great Wall of the Qi state, dating back over 2,600 years, now stands as a popular attraction for visitors exploring Jinan, capital of Shandong Province. In Ganzhou, Jiangxi Province, the “Long March–themed” red tourism is widely acclaimed, allowing visitors to retrace the Red Army’s Long March route in the 1930s. Many visitors roam along the Grand Canal to feel the Chinese wisdom of the Xiejia Dam in Cangzhou, Hebei Province, for locals adopted the sticky rice grouting technique while building the dam some 200 years ago. The picturesque Wuxia Gorge, one of the Three Gorges on the Yangtze River, makes visitors linger.

关键词： visitors Grand Yangtze

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Indirubin alleviates retinal neurodegeneration through the regulation of PI3K/AKT signaling

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Journal of Biomedical Research 2024年第3期38卷 256-268页

作者： Huan Li huiying Zhang Lushu Chen Yaming Shen Yuan Cao Xiumiao Li Jin Yao Department of Ophthalmology the Affiliated Eye Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Ophthalmology the Fourth School of Clinical MedicineNanjing Medical UniversityNanjingJiangsu 210029China

Retinal neurodegenerative disease is a leading cause of blindness among the elderly in developed countries,including glaucoma,diabetic retinopathy,traumatic optic neuropathy and optic neuritis,etc.The current clinical treatment is not very effective.We investigated indirubin,one of the main bioactive components of the traditional Chinese medicine Danggui Longhui Pill,in the present study for its role in retinal neurodegeneration.Indirubin exhibited no detectable tissue toxicity in vivo or cytotoxicity in vitro.Moreover,indirubin improved visual function and ameliorated retinal neurodegeneration in mice after optic nerve crush injury in vivo.Furthermore,indirubin reduced the apoptosis of retinal ganglion cells induced by oxidative stress in vitro.In addition,indirubin significantly suppressed the increased production of intracellular reactive oxygen species and the decreased activity of superoxide dismutase induced by oxidative stress.Mechanically,indirubin played a neuroprotective role by regulating the PI3K/AKT/BAD/BCL-2 signaling.In conclusion,indirubin protected retinal ganglion cells from oxidative damage and alleviated retinal neurodegeneration induced by optic nerve crush injury.The present study provides a potential therapeutic medicine for retinal neurodegenerative diseases.

关键词： retinal neurodegenerative disease oxidative stress PI3K/AKT retinal ganglion cell apoptosis

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The Opening Ceremony of the 4Oth China Harbin International Ice and Snow Festival&China-France Yearof Culture and Tourism

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China & The World Cultural Exchange 2024年第1期 22-25页

作者： Zhou huiying 不详

The opening ceremony of the 4Oth China Harbin International Iceand Snow Festival&China-FranceYearof Cultureand Tourism was held in Harbin Ice and Snow Worldon January 5,2024.Under the darkening sky in the evening,the dazzling lights illuminated Harbin Iceand Snow World.In the charming lighting and dynamic music,tourists from all over the world gathered together to enjoy the magnificent ice and snow sceneryand sharethe unique charm of the feast of Heilongjiang.

关键词： Ice lighting lights

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