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Identification,characterization,and verification of miR399 target gene in grape

Horticultural Plant Journal 2024年第1期10卷 91-102页

作者： Maosong Pei Hainan Liu Tonglu Wei huiying Jin Yihe Yu Mengting Ma Xiaomeng Song Rundong Dai Dalong guo Henan Engineering Technology Research Center of Quality Regulation and Controlling of Horticultural Plants College of Horticulture and Plant ProtectionHenan University of Science and TechnologyLuoyangHenan 471023China

The microRNA miR399 plays an important role in phosphorus signal transduction pathways in plants.Previously,miR399 was shown to be closely associated with berry ripening in grape(Vitis vinifera).The objective of the present study was to elucidate the evolutionary characteristics of the miR399 gene family in grape and to verify the cleavage effect on the target genes.Grape miR399s were identified by miRNA sequencing and retrieval from the miRBase database.The mature sequences and precursor sequences were subjected to phylogenetic analysis to reconstruct evolutionary trees,as well as secondary structure analysis of the precursor sequence,and prediction of target genes.The cisacting elements in the miR399 promoter were predicted and the cleavage effect of grape miR399b on its target genes was verified.The grape miR399 family comprised nine precursor sequences and nine mature sequences.The precursor sequences formed a typical and stable stem—loop structure.The minimum folding free energy ranged from-55.70 kcal·mol^(-1)to-37.40 kcal·mol^(-1).Multiple sequence alignment revealed that the miR399 family was highly conserved.The grape miR399 family was phylogenetically closely related to peach,apple,and citrus miR399s.Grape miR399s were predicted to target inorganic phosphate transporter 1—3,phospholipase D delta-like,and beta-glucuronosyltransferase.The cleavage effect of grape miR399b on the target genes was verified by means of a dual-luciferase assay and 5’RLM-RACE.Histochemical GUS staining showed that the promoter activity of miR399b was promoted by GA3treatment.

关键词： Grape miR399 Phosphorus metabolism Early ripening

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FERM domain-containing protein FRMD6 activates the mTOR signaling pathway and promotes lung cancer progression

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Frontiers of Medicine 2023年第4期17卷 714-728页

作者： Tianzhuo Wang huiying guo Lei Zhang Miao Yu Qianchen Li Jing Zhang Yan Tang Hongquan Zhang Jun Zhan Program for Cancer and Cell Biology Department of Human AnatomyHistology and EmbryologySchool of Basic Medical SciencesBeijing 100191China Peking University International Cancer Institute Beijing 100191China MOE Key Laboratory of Carcinogenesis and Translational Research and State Key Laboratory of Natural and Biomimetic Drugs Peking University Health Science CenterBeijing 100191China

FRMD6,a member of the 4.1 ezrin-radixin-moesin domain-containing protein family,has been reported to inhibit tumor progression in multiple cancers.Here,we demonstrate the involvement of FRMD6 in lung cancer progression.We find that FRMD6 is overexpressed in lung cancer tissues relative to in normal lung tissues.In addition,the enhanced expression of FRMD6 is associated with poor outcomes in patients with lung squamous cell carcinoma(n=75,P=0.0054)and lung adenocarcinoma(n=94,P=0.0330).Cell migration and proliferation in vitro and tumor formation in vivo are promoted by FRMD6 but are suppressed by the depletion of FRMD6.Mechanistically,FRMD6 interacts and colocalizes with mTOR and S6K,which are the key molecules of the mTOR signaling pathway.FRMD6 markedly enhances the interaction between mTOR and S6K,subsequently increasing the levels of endogenous pS6K and downstream pS6 in lung cancer cells.Furthermore,knocking out FRMD6 inhibits the activation of the mTOR signaling pathway in Frmd6^(−/−)gene KO MEFs and mice.Altogether,our results show that FRMD6 contributes to lung cancer progression by activating the mTOR signaling pathway.

关键词： FRMD6 lung cancer mTOR pathway

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The leaching of additive-derived flame retardants(FRs) from plastics in avian digestive fluids:The significant risk of highly lipophilic FRs

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Journal of Environmental Sciences 2019年第11期31卷 200-207页

作者： huiying guo Xiaobo Zheng Shuling Ru Xiaojun Luo Bixian Mai State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Protection and Resources Utilization Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640 China College of Resources and Environment South China Agricultural University Guangzhou 510642 China University of the Chinese Academy of Sciences Beijing 100049 China

The exposure to plastic debris and associated pollutants for wildlife is of urgent concern,but little attention has been paid on the transfer of plastic additives from plastic debris to organisms.In the present study,the leaching of incorporated flame retardants (FRs),including polybrominated diphenyl ethers (PBDEs),alternative brominated FRs (AFRs),and phosphate flame retardants (PFRs),from different sizes of recycled acrylonitrile-butadiene-styrene (ABS) polymer were investigated in avian digestive fluids.The impact of co-ingested sediment on the leaching of additive-derived FRs in digestive fluids was also explored.In the recycled ABS,BDE 209 (715 μg/g) and 1,2-bis(2,4,6-tribromophenoxy) ethane (BTBPE,1766 μg/g) had the highest concentrations among all target FRs.The leaching proportions of FRs were higher in finer sizes of ABS.The leaching proportions of FRs from recycled ABS increased with elevated logKow of FRs.In the tests with coexisted ABS and sediment,hexato deca-BDEs,BTBPE,and decabromodiphenyl ethane (DBDPE) migrated from ABS to sediment,which resulted in the less bioaccessible fractions of these FRs in gut fluids.More lipophilic chemicals tended to be adsorbed by sediment from ABS.The results suggest the migration of additive-derived FRs from plastics to other indigestible materials in digestive fluids.The findings in this study provide insights into the transfer of additive-derived FRs from plastics to birds,and indicate the significant contribution of FR-incorporated plastics to bioaccumulation of highly lipophilic FRs.

关键词： Recycled plastics Digestive fluids Flame retardants Leaching

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A centromere map based on super pan-genome highlights the structure and function of rice centromeres

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Journal of Integrative Plant Biology 2024年第2期66卷 196-207页

作者： Yang Lv Congcong Liu Xiaoxia Li Yueying Wang huiying He Wenchuang He Wu Chen Longbo Yang Xiaofan Dai Xinglan Cao Xiaoman Yu Jiajia Liu Bin Zhang Hua Wei Hong Zhang Hongge Qian Chuanlin Shi Yue Leng Xiangpei Liu Mingliang guo Xianmeng Wang Zhipeng Zhang Tianyi Wang Bintao Zhang Qiang Xu Yan Cui Qianqian Zhang Qiaoling Yuan Noushin Jahan Jie Ma Xiaoming Zheng Yongfeng Zhou Qian Qian Longbiao guo Lianguang Shang State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006China Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China Yazhouwan National Laboratory No.8 Huanjin RoadYazhou DistrictSanya City 572024China National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China

Rice(Oryza sativa)is a significant crop worldwide with a genome shaped by various evolutionary factors.Rice centromeres are crucial for chromosome segregation,and contain some unreported genes.Due to the diverse and complex centromere region,a comprehensive understanding of rice centromere structure and function at the population level is needed.We constructed a high-quality centromere map based on the rice super pangenome consisting of a 251-accession panel comprising both cultivated and wild species of Asian and African rice.We showed that rice centromeres have diverse satellite repeat CentO,which vary across chromosomes and subpopulations,reflecting their distinct evolutionary patterns.We also revealed that long terminal repeats(LTRs),especially young Gypsy-type LTRs,are abundant in the peripheral CentO-enriched regions and drive rice centromere expansion and evolution.Furthermore,high-quality genome assembly and complete telomere-to-telomere(T2T)reference genome enable us to obtain more centromeric genome information despite mapping and cloning of centromere genes being challenging.We investigated the association between structural variations and gene expression in the rice centromere.A centromere gene,OsMAB,which positively regulates rice tiller number,was further confirmed by expression quantitative trait loci,haplotype analysis and clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein9 methods.By revealing the new insights into the evolutionary patterns and biological roles of rice centromeres,our finding will facilitate future research on centromere biology and crop improvement.

关键词： centromere super pan-genome CentoO satellite repeat rice

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Uncovering key salt-tolerant regulators through a combined eQTL and GWAS analysis using the super pan-genome in rice

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National Science Review 2024年第4期11卷 55-69页

作者： Hua Wei Xianmeng Wang Zhipeng Zhang Longbo Yang Qianqian Zhang Yilin Li huiying He Dandan Chen Bin Zhang Chongke Zheng Yue Leng Xinglan Cao Yan Cui Chuanlin Shi Yifan Liu Yang Lv Jie Ma Wenchuang He Xiangpei Liu Qiang Xu Qiaoling Yuan Xiaoman Yu Tianyi Wang Hongge Qian Xiaoxia Li Bintao Zhang Hong Zhang Wu Chen Mingliang guo Xiaofan Dai Yuexing Wang Xiaoming Zheng Longbiao guo Xianzhi Xie Qian Qian Lianguang Shang Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural Sciences Institute of Wetland Agriculture and Ecology Shandong Academy of Agricultural Sciences State Key Laboratory of Rice Biology China National Rice Research Institute National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Science Chinese Academy of Agricultural Sciences Yazhouwan National Laboratory

For sessile plants, gene expression plays a pivotal role in responding to salinity stress by activating or suppressing specific genes. However, our knowledge of genetic variations governing gene expression in response to salt stress remains limited in natural germplasm. Through transcriptome analysis of the Global Mini-Core Rice Collection consisting of a panel of 202 accessions, we identified 22 345 and 27 610expression quantitative trait loci associated with the expression of 7787 and 9361 e Genes under normal and salt-stress conditions, respectively, leveraging the super pan-genome map. Notably, combined with genome-wide association studies, we swiftly pinpointed the potential candidate gene STG5—a major salt-tolerant locus known as q STS5. Intriguingly, STG5 is required for maintaining Na+/K+homeostasis by directly regulating the transcription of multiple members of the Os HKT gene family. Our study sheds light on how genetic variants influence the dynamic changes in gene expression responding to salinity stress and provides a valuable resource for the mining of salt-tolerant genes in the future.

关键词： super pan-genome expression quantitative trait loci GWAS salt tolerance rice

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长期预防性治疗结合按需治疗控制遗传性血管性水肿——2021版WAO/EAACI国际遗传性血管性水肿管理指南解读

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中华预防医学杂志 2024年第5期58卷 698-705页

作者：汪慧英郭胤仕姚我程雷浙江大学医学院附属第二医院变态反应科杭州310009 上海交通大学医学院附属仁济医院变态反应(过敏)科上海200001 南京医科大学第一附属医院江苏省人民医院耳鼻咽喉科/过敏诊疗中心南京210029

2021版世界过敏组织(WAO)/欧洲过敏与临床免疫学会(EAACI)国际遗传性血管性水肿(hereditary angioedema,HAE)管理指南主要以高质量的随机对照试验为依据,对HAE进行临床分型,就HAE的按需治疗、短期预防性治疗和长期预防性治疗药物给予分... 详细信息

2021版世界过敏组织(WAO)/欧洲过敏与临床免疫学会(EAACI)国际遗传性血管性水肿(hereditary angioedema,HAE)管理指南主要以高质量的随机对照试验为依据,对HAE进行临床分型,就HAE的按需治疗、短期预防性治疗和长期预防性治疗药物给予分级推荐,并针对不同HAE患病人群提供管理策略。本文主要对HAE的短期、长期预防性治疗和按需治疗部分进行解读,并补充最新临床证据,以期为HAE的长期管理提供参考。

关键词：遗传性血管性水肿按需治疗长期预防性治疗指南

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人工智能驱动教育变革的伦理风险及其解蔽之路

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中国电化教育 2024年第4期 25-31页

作者：郭颢江楠江宏刘忠骅邓辉映黑龙江教师发展学院黑龙江哈尔滨150080 重庆第二师范学院重庆400065 重庆市教育科学研究院重庆400015 重庆市第八中学校重庆400030

人工智能驱动教育变革给教育带来双重影响,使教育中的机会与风险共存。尽管人工智能技术给教育带来强大优势,能够增强教育主体的能力、记录完整的教育信息、推动人的个性发展、以及提高教育效率。但随着技术向度的增强与工具理性思维的... 详细信息

人工智能驱动教育变革给教育带来双重影响,使教育中的机会与风险共存。尽管人工智能技术给教育带来强大优势,能够增强教育主体的能力、记录完整的教育信息、推动人的个性发展、以及提高教育效率。但随着技术向度的增强与工具理性思维的介入,这一过程也面临不同维度的伦理风险。人工智能驱动教育变革的伦理风险主要表现为主体风险、安全风险、算法风险和技术滥用四方面。解蔽人工智能裹挟下的四重风险,推动人工智能技术在教育变革中的价值实现,需回归以人为本的价值理念,强化教育主体地位;建立依法规约的伦理体系,加强信息安全管理;遵循算法使用的伦理准则,促进个体生命发展;内化德性为先的伦理意识,健全技术变革边界。

关键词：人工智能教育变革伦理风险解蔽

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Preoperatively predicting vessels encapsulating tumor clusters in hepatocellular carcinoma:Machine learning model based on contrast-enhanced computed tomography

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World Journal of Gastrointestinal Oncology 2024年第3期16卷 857-874页

作者： Chao Zhang Hai Zhong Fang Zhao Zhen-Yu Ma Zheng-Jun Dai guo-Dong Pang Department of Radiology The Second Hospital of Shandong UniversityJinan 250033Shandong ProvinceChina Department of Radiology Qilu Hospital of Shandong UniversityJinan 250014Shandong ProvinceChina Department of Radiology Linglong Yingcheng HospitalYantai 265499Shandong ProvinceChina Department of Scientific Research Huiying Medical Technology Co.LtdBeijing 100192China

BACKGROUND Recently,vessels encapsulating tumor clusters(VETC)was considered as a distinct pattern of tumor vascularization which can primarily facilitate the entry of the whole tumor cluster into the bloodstream in an invasion independent manner,and was regarded as an independent risk factor for poor prognosis in hepatocellular carcinoma(HCC).AIM To develop and validate a preoperative nomogram using contrast-enhanced computed tomography(CECT)to predict the presence of VETC+in HCC.METHODS We retrospectively evaluated 190 patients with pathologically confirmed HCC who underwent CECT scanning and immunochemical staining for cluster of differentiation 34 at two medical centers.Radiomics analysis was conducted on intratumoral and peritumoral regions in the portal vein phase.Radiomics features,essential for identifying VETC+HCC,were extracted and utilized to develop a radiomics model using machine learning algorithms in the training set.The model’s performance was validated on two separate test sets.Receiver operating characteristic(ROC)analysis was employed to compare the identified performance of three models in predicting the VETC status of HCC on both training and test sets.The most predictive model was then used to constructed a radiomics nomogram that integrated the independent clinical-radiological features.ROC and decision curve analysis were used to assess the performance characteristics of the clinical-radiological features,the radiomics features and the radiomics nomogram.RESULTS The study included 190 individuals from two independent centers,with the majority being male(81%)and a median age of 57 years(interquartile range:51-66).The area under the curve(AUC)for the combined radiomics features selected from the intratumoral and peritumoral areas were 0.825,0.788,and 0.680 in the training set and the two test sets.A total of 13 features were selected to construct the Rad-score.The nomogram,combining clinicalradiological and combined radiomics features could accurately predict VETC+in all three sets,with AUC

关键词： Hepatocellular carcinoma Vessels encapsulating tumor clusters Intratumoral and peritumoral regions Radiomics features Nomog

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基于动态异构网络的股价预测

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计算机应用研究 2024年第7期41卷 2126-2133页

作者：韩忠明孟怡新郭惠莹郭苗苗毛雅俊北京工商大学计算机与人工智能学院北京100048

股票预测通常被形式化为非线性的时间序列预测任务,但很少有研究者试图通过技术面数据去系统地揭示股票市场内在结构,例如股票上涨或下跌背后的原因可能是业务领域之间的合作或冲突,这些额外信息的增加有助于判断股票的未来趋势。为了... 详细信息

股票预测通常被形式化为非线性的时间序列预测任务,但很少有研究者试图通过技术面数据去系统地揭示股票市场内在结构,例如股票上涨或下跌背后的原因可能是业务领域之间的合作或冲突,这些额外信息的增加有助于判断股票的未来趋势。为了充分真实刻画股票市场的交易状态,表达股票之间显式或隐式的关系,提出一种基于动态异构网络的股价预测模型sDHN(stock dynamic heterogeneous network),综合股票以及所属行业和地域,将其建模为动态异构网络。该模型在网络上引入动态时序特征,创新融合股票节点的四种不同技术层面的相似性图,生成富信息异构图,最后聚合不同元路径中隐含的语义信息生成嵌入,从异构图的角度充分探索股票之间的潜在关联。此外,在三个真实世界的股票数据集上进行了大量实验,所提出的模型准确率比所有基线模型均高出5%~34%,F 1-score则高出11.5%~37%,并且在图解释上证明了该方法的有效性。

关键词：股票预测异构网络图相似性

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新型Sn-Zn系焊铝锡膏的制备

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材料与冶金学报 2024年第1期23卷 36-41,47页

作者：宁馨锋郭瑛刘慧颖郭天浩马海涛大连理工大学材料科学与工程学院辽宁大连116024

研制了一种新型Sn-Zn系焊铝锡膏.根据焊铝锡膏成分组成特点,以Sn-Zn系无铅锡粉为基础,对助焊膏中活性剂、活性盐、表面活性剂和溶剂等添加剂进行筛选,通过正交试验对其组分进行优化并制备出焊铝锡膏.结果表明:活性剂部分,有机胺A质量的... 详细信息

研制了一种新型Sn-Zn系焊铝锡膏.根据焊铝锡膏成分组成特点,以Sn-Zn系无铅锡粉为基础,对助焊膏中活性剂、活性盐、表面活性剂和溶剂等添加剂进行筛选,通过正交试验对其组分进行优化并制备出焊铝锡膏.结果表明:活性剂部分,有机胺A质量的增加会降低钎料在240℃和260℃下的润湿性;活性盐部分,锌盐和亚锡盐有利于扩大钎料铺展面积,但添加铵盐会降低钎料的润湿性;表面活性剂部分,添加OP-10的实验效果要优于添加油酸酰胺的实验效果.基于以上研究结果自制出的焊铝锡膏可用于低温钎焊1060铝,焊接质量良好,界面处未见明显焊接缺陷,且焊铝锡膏具有良好的存储稳定性.研究结果可为铝合金钎焊用焊铝锡膏的应用和开发提供理论和技术支持.

关键词：铝合金焊铝锡膏助焊膏正交试验

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