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miR-9通过下调PTEN促进喉癌细胞的增殖效果

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长春中医药大学学报 2020年第5期36卷 981-983页

作者：熊向菁王青海李俊娟付海生王淼舟刘辉琦西宁市第一人民医院耳鼻喉科西宁810000 青海大学医学院附属医院肿瘤内科西宁810001 青海大学医学院基础部西宁810016

目的探讨miR-9通过下调磷酸酶与张力蛋白同源缺失基因(PTEN)对喉癌细胞增殖的影响及其作用机制。方法选择喉癌细胞系HEP-2细胞随机分为空白组、阴性对照组与miR-9组。阴性对照组转染miR-9 NC(2μg),miR-9组转染miR-9 mimic(2μg),空白... 详细信息

目的探讨miR-9通过下调磷酸酶与张力蛋白同源缺失基因(PTEN)对喉癌细胞增殖的影响及其作用机制。方法选择喉癌细胞系HEP-2细胞随机分为空白组、阴性对照组与miR-9组。阴性对照组转染miR-9 NC(2μg),miR-9组转染miR-9 mimic(2μg),空白组不进行转染。采用qRT-PCR检测各组miR-9表达水平,MTT法检测细胞增殖指数,流式细胞术检测细胞凋亡指数,Western Blot检测PTEN表达水平。结果转染后24 h与48 h,miR-9组的miR-9表达水平显著高于空白组与阴性对照组(P0.05)。结论miR-9能通过下调PTEN的表达,促进喉癌细胞的增殖,抑制其凋亡,从而发挥促癌作用。

关键词： miR-9 喉癌细胞增殖细胞凋亡

Stem cell microencapsulation maintains stemness in inflammatory microenvironment

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International Journal of Oral Science 2022年第4期14卷 549-560页

作者： Yajun Zhao Yilin Shi huiqi Yang Mengmeng liu Lanbo Shen Shengben Zhang Yue liu Jie Zhu Jing Lan Jianhua Li Shaohua Ge Department of Implantology&Periodontology School and Hospital of StomatologyCheeloo College of MedicineShandong University&Shandong Key Laboratory of Oral Tissue Regeneration&Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration&Shandong Provincial Clinical Research Center for Oral DiseasesJinanChina Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research College of StomatologyXi'an Jiaotong UniversityXi'anChina Airong Plastic Surgery Hospital JinanChina

Maintaining the stemness of the transplanted stem cell spheroids in an inflammatory microenvironment is challenging but important in regenerative medicine. Direct delivery of stem cells to repair periodontal defects may yield suboptimal effects due to the complexity of the periodontal inflammatory environment. Herein, stem cell spheroid is encapsulated by interfacial assembly of metal-phenolic network(MPN) nanofilm to form a stem cell microsphere capsule. Specifically, periodontal ligament stem cells(PDLSCs) spheroid was coated with FeⅢ/tannic acid coordination network to obtain spheroid@[FeⅢ-TA] microcapsules. The formed biodegradable MPN biointerface acted as a cytoprotective barrier and exhibited antioxidative, antibacterial and anti-inflammatory activities, effectively remodeling the inflammatory microenvironment and maintaining the stemness of PDLSCs. The stem cell microencapsulation proposed in this study can be applied to multiple stem cells with various functional metal ion/polyphenol coordination, providing a simple yet efficient delivery strategy for stem cell stemness maintenance in an inflammatory environment toward a better therapeutic outcome.

关键词： inflammatory protective maintain

HSP90β干扰和过表达慢病毒载体的构建及其在骨肉瘤细胞Saos-2中的表达

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现代肿瘤医学 2020年第7期28卷 1057-1062页

作者：舒雄刘辉琦杰永生郑蕊綦惠陈磊靳少锋冉宇靓北京积水潭医院/北京市创伤骨科研究所北京100035 青海大学青海西宁810016 国家癌症中心/国家肿瘤临床医学研究中心/中国医学科学院北京协和医学院肿瘤医院北京100021

目的:获得热休克蛋白90β(HSP90β)基因干扰和过表达慢病毒表达系统,并检测其在人骨肉瘤细胞株Saos-2中的表达水平。方法:设计合成shRNA,以慢病毒表达质粒构建HSP90β干扰和过表达载体,酶切电泳、测序技术鉴定载体构建是否成功。重组病... 详细信息

目的:获得热休克蛋白90β(HSP90β)基因干扰和过表达慢病毒表达系统,并检测其在人骨肉瘤细胞株Saos-2中的表达水平。方法:设计合成shRNA,以慢病毒表达质粒构建HSP90β干扰和过表达载体,酶切电泳、测序技术鉴定载体构建是否成功。重组病毒转染H1299细胞,以嘌呤霉素筛选稳定转染的Saos-2细胞,通过荧光显微镜观察计数获得转染效率。将感染好的细胞分为干扰NC组(NEG-shRNA)、干扰组(shRNA-HSP90β)、过表达NC对照组(NEG-pEZ)及过表达组(pEZ-HSP90β)。通过qRT-PCR与Western blotting分别从mRNA和蛋白表达水平验证目的基因的干扰和过表达水平。结果:插入慢病毒表达载体的基因片段与目的基因的碱基序列完全一致。病毒包装成功后,嘌呤霉素最小致死浓度1μg/ml,感染复数200,感染人Saos-2的感染效率达80%,其中shRNA-HSP90β组干扰效率为86.35%,pEZ-HSP90β组的mRNA相对表达量增加2. 8倍。进一步研究发现,shRNA-HSP90β组较NEG-shRNA组中HSP90β蛋白表达明显降低,p EZ-HSP90β组较NEG-p EZ组HSP90β蛋白表达增加。结论:HSP90β基因干扰和过表达慢病毒载体构建成功,并能够在Saos-2中稳定表达。

关键词：骨肉瘤细胞 HSP90β 慢病毒载体 RNA干扰过表达

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合肥瑶海区深化街道体制改革

中国机构改革与管理 2019年第10期 37-37页

作者：李东平刘会启合肥市瑶海区委编办

合肥市瑶海区以推进“一个窗口管服务、一张网格管治理、一列清单管权责、一支队伍管执法”为抓手深化街道体制改革。2019年以来,安徽省合肥市瑶海区针对街道当前人力资源分散、网格设置过多、职责权限边界不清、城市管理精细化水平不... 详细信息

合肥市瑶海区以推进“一个窗口管服务、一张网格管治理、一列清单管权责、一支队伍管执法”为抓手深化街道体制改革。2019年以来,安徽省合肥市瑶海区针对街道当前人力资源分散、网格设置过多、职责权限边界不清、城市管理精细化水平不高等问题,从适应街道工作特点及便民服务需要的角度出发,以推进“一个窗口管服务、一张网格管治理、一列清单管权责、一支队伍管执法”为抓手深化街道体制改革。

关键词：街道工作体制改革瑶海区合肥市便民服务管理精细化人力资源职责权限

One step synthesis of efficient red emissive carbon dots and their bovine serum albumin composites with enhanced multi-photon fluorescence for in vivo bioimaging

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Light(Science & Applications) 2022年第5期11卷 955-968页

作者： huiqi Zhang Gang Wang Zhiming Zhang Josh Haipeng Lei Tzu-Ming liu Guichuan Xing Chu-Xia Deng Zikang Tang Songnan Qu Joint Key Laboratory of the Ministry of Education Institute of Applied Physics and Materials EngineeringUniversity of MacaoMacao 999078China Institute of Translational Medicine Faculty of Health SciencesUniversity of MacaoMacao 999078China MoE Frontiers Science Center for Precision Oncology University of MacaoMacao 999078China Cancer Center Faculty of Health SciencesUniversity of MacaoMacao 999078China

Efficient red emissive carbon dots(CDs)in aqueous solutions are very scarce for high performance bioimaging *** this work,we report a one-step solvothermal treatment to synthesize pure red emissive CDs(FA-CDs)from citric acid and urea in formic acid without complicated purification *** quantum yield(PLQY)of 43.4%was observed in their dimethyl sulfoxide *** PLQY up to 21.9%in aqueous solutions was achieved in their bovine serum albumin(BSA)composites(FA-CDs@BSA)with significantly enhanced multiphoton *** strong surface electron-withdrawing structure of FA-CDs caused by the high content of C=O groups contributes for their pure red *** to the significantly enhanced single and multi-photon red fluorescence and enlarged particle sizes after composing with BSA,in vivo tumor imaging and two-photon fluorescence imaging of blood vessels in mouse ear have been realized via intravenous injection of FA-CDs@BSA aqueous solutions.

关键词： photon pure synthesis

肝癌干细胞CD90^+和ESA^+亚群生物学特性研究

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大连医科大学学报 2018年第5期40卷 394-398,407页

作者：刘辉琦舒雄孙立新遇珑孙力超杨治华冉宇靓国家癌症中心/国家肿瘤临床医学研究中心/中国医学科学院北京协和医学院肿瘤医院细胞及分子生物学实验室北京100021

目的研究肝癌干细胞不同亚群CD90+和ESA+细胞的生物学特性,为肝癌的治疗及预后判定提供新的思路。方法采用活细胞流式细胞术检测肝癌细胞系MHCC97L中肝癌干细胞标志物CD90、ESA的表达情况,并分选ESA+,CD90+细胞,通过甲基纤维素成球实验... 详细信息

目的研究肝癌干细胞不同亚群CD90+和ESA+细胞的生物学特性,为肝癌的治疗及预后判定提供新的思路。方法采用活细胞流式细胞术检测肝癌细胞系MHCC97L中肝癌干细胞标志物CD90、ESA的表达情况,并分选ESA+,CD90+细胞,通过甲基纤维素成球实验、Transwell、CCK8法进行体外生物学特征研究。结果肝癌细胞系MHCC97L中CD90+和ESA+细胞表达比例分别为2. 37%和3. 70%。ESA+细胞成球率明显高于ESA-细胞[成球率(35. 6±2. 1)%vs.(9. 6±1. 4)%,P <0. 01],CD90+细胞成球率明显高于CD90-细胞[成球率(29. 2±1. 3)%vs.(7. 4±0. 6)%](P <0. 01),ESA+细胞成球率明显高于CD90+细胞且ESA+细胞sphere球体积明显大于CD90+细胞体积; ESA+细胞较ESA-细胞侵袭能力提高2. 12倍[(282±15. 1 vs. 133±12. 4),P <0. 01],CD90+细胞较CD90-细胞侵袭能力提高2. 04倍[(231±18. 1 vs. 113±10. 2),P <0. 01]。CD90+细胞耐药性明显强于ESA+细胞[IC50(0. 98μg/m L vs. 0. 36μg/m L)],ESA+细胞增殖能力明显强于CD90+细胞。结论肝癌组织中存在不同的干细胞亚群ESA+细胞及CD90+细胞,这些亚群具有不同的生物学特性,影响肝癌的耐药、侵袭,了解肝癌中不同干细胞亚群的表达情况可用于指导临床个体化治疗。

关键词：肝癌肿瘤干细胞 CD90 ESA

MicroRNA-34c-5p provokes isoprenaline-induced cardiac hypertrophy by modulating autophagy via targeting ATG4B

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Acta Pharmaceutica Sinica B 2022年第5期12卷 2374-2390页

作者： Yuhong Zhang Yanqing Ding Min Li Jing Yuan Youhui Yu Xueying Bi huiqi Hong Jiantao Ye Peiqing liu Department of Pharmacology and Toxicology School of Pharmaceutical SciencesNational and Local United Engineering Lab of Druggability and New Drugs EvaluationSun Yat-sen UniversityGuangzhou 510006China State Key Laboratory of Oncology in Southern China Collaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer CenterGuangzhou 510006China

Pathological cardiac hypertrophy serves as a significant foundation for cardiac dysfunction and heart failure. Recently, growing evidence has revealed that microRNAs(miRNAs) play multiple roles in biological processes and participate in cardiovascular diseases. In the present research, we investigate the impact of miRNA-34 c-5 p on cardiac hypertrophy and the mechanism involved. The expression of miR-34 c-5 p was proved to be elevated in heart tissues from isoprenaline(ISO)-infused mice. ISO also promoted miR-34 c-5 p level in primary cultures of neonatal rat cardiomyocytes(NRCMs). Transfection with miR-34 c-5 p mimic enhanced cell surface area and expression levels of foetal-type genes atrial natriuretic factor(Anf) and β-myosin heavy chain(β-Mhc) in NRCMs. In contrast, treatment with miR-34 c-5 p inhibitor attenuated ISO-induced hypertrophic responses. Enforced expression of miR-34 c-5 p by tail intravenous injection of its agomir led to cardiac dysfunction and hypertrophy in mice, whereas inhibiting miR-34 c-5 p by specific antagomir could protect the animals against ISO-triggered hypertrophic abnormalities. Mechanistically, miR-34 c-5 p suppressed autophagic flux in cardiomyocytes, which contributed to the development of hypertrophy. Furthermore, the autophagy-related gene 4 B(ATG4 B) was identified as a direct target of miR-34 c-5 p, and miR-34 c-5 p was certified to interact with 3’untranslated region of Atg4 b mRNA by dual-luciferase reporter assay. miR-34 c-5 p reduced the expression of ATG4 B, thereby resulting in decreased autophagy activity and induction of hypertrophy. Inhibition of miR-34 c-5 p abolished the detrimental effects of ISO by restoring ATG4 B and increasing autophagy. In conclusion, our findings illuminate that miR-34 c-5 p participates in ISO-induced cardiac hypertrophy, at least partly through suppressing ATG4 B and autophagy. It suggests that regulation of miR-34 c-5 p may offer a new way for handling hypertrophy-related cardiac dysfuncti

关键词： Pathological cardiac hypertrophy Isoprenaline miR-34c-5p ATG4B LC3 Autophagy Autophagic flux Neonatal rat cardiomyocytes Mice

Decomposition of dioxin-like components in a DBD reactor combined with Hg/Ar electrodeless ultraviolet

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Plasma Science and Technology 2020年第3期22卷 34-39页

作者： Weixuan ZHAO Liping LIAN Yanpei WU Yanghaichao liu Renxi ZHANG Gang LUO huiqi HOU Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention(LAP3) Institute of Environmental ScienceFudan UniversityShanghai 200433People’s Republic of China National Engineering Laboratory for VOCs Pollution Control Material&Technology University of Chinese Academy of SciencesBeijing 100049People’s Republic of China

A new combined reactor with Hg/Ar electrodeless ultraviolet(EDUV)activated by DBD for 3,4-dichlorodiphenyl ether abatement is *** effect of specific input energy and feeding gas component on 3,4-dichlorodiphenyl ether removal efficiency has been *** with a single DBD system,this new combined process performed a significant promotion on 3,4-dichlorodiphenyl ether *** results verified that active oxygen clearly contributed to the synergistic activity of DBD-EDUV *** of emission spectra showed that UV radiation of 253.7 nm could be detected in the DBD-EDUV ***,the products of DBD-EDUV process were analyzed via gas chromatographymass spectrometer(GC-MS)to reveal involved decomposition mechanism.

关键词： dielectric barrier discharge(DBD) dioxin-like 3,4-dichlorodiphenyl ether EDUV photolysis

Structure-guided engineering of transcriptional activator XYR1 for inducer-free production of lignocellulolytic enzymes in Trichoderma reesei

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Synthetic and Systems Biotechnology 2023年第4期8卷 732-740页

作者： Qinqin Zhao Zezheng Yang Ziyang Xiao Zheng Zhang Jing Xing huiqi Liang Liwei Gao Jian Zhao Yinbo Qu Guodong liu State Key Laboratory of Microbial Technology Shandong UniversityQingdao266237China Taishan College Shandong UniversityQingdao266237China Tobacco Research Institute of Chinese Academy of Agricultural Sciences Qingdao266101China

The filamentous fungus Trichoderma reesei is widely used for the production of lignocellulolytic enzymes in ***1 is the major transcriptional activator of cellulases and hemicellulases in ***,rational engineering of XYR1 for improved lignocellulolytic enzymes production has been limited by the lack of structure ***,alanine 873 was identified as a new potential target for the engineering of XYR1 based on its structure predicted by *** mutation of this residue to tyrosine enabled significantly enhanced production of xylanolytic enzymes in the medium with cellulose as the carbon ***,xylanase and cellulase production increased by 56.7-and 3.3-fold,respectively,when glucose was used as the sole carbon *** both conditions,the improvements of lignocellulolytic enzyme production were higher than those in the previously reported V821F *** the enriched hemicellulases and cellulases,the crude enzymes secreted by the A873Y mutant strain produced 51%more glucose and 52%more xylose from pretreated corn stover than those of the parent *** results provide a novel strategy for engineering the lignocellulolytic enzyme-producing capacity of ***,and would be helpful for understanding the molecular mechanisms of XYR1 regulation.

关键词： Xylanase Cellulase Trichoderma reesei Transcription factor Genome editing