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Selenium-oxidizing Agrobacterium sp.T3F4 decreases arsenic uptake by Brassica rapa *** a native polluted soil

Journal of Environmental Sciences 2024年第4期138卷 506-515页

作者： Lijin An Chunzhi Zhou Lipeng Zhao Ao Wei Yiting Wang huimin cui Shixue Zheng State Key Laboratory of Agricultural Microbiology College of Life Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China

Toxic arsenic(As)and trace element selenium(Se)are transformed by microorganisms but their complex interactions in soil-plant systems have not been fully *** Asand Se-oxidizing bacterium,Agrobacterium sp.T3F4,was applied to a native seleniferous As-polluted soil to investigate As/Se uptake by the vegetable Brassica rapa *** As-Se interaction as mediated by strain *** Se content in the aboveground plants was significantly enhanced by 34.1%,but the As content was significantly decreased by 20.5% in the T3F4-inoculated pot culture compared to the control(P<0.05).Similar result was shown in treatment with additional 5 mg/kg of Se(IV)in *** addition,the As contents in roots were significantly decreased by more than 35% under T3F4 or Se(IV)treatments(P<0.05).Analysis of As-Se-bacterium interaction in a soil simulation experiment showed that the bioavailability of Se significantly increased and As was immobilized with the addition of the T3F4strain(P<0.05).Furthermore,an As/Se co-exposure hydroponic experiment demonstrated that As uptake and accumulation in plants was reduced by increasing Se(IV)*** 50% growth inhibition concentration(IC50)values for As in plants were increased about one-fold and two-fold under co-exposure with 5 and 10μmol/L Se(IV),*** conclusion,strain T3F4 improves Se uptake but decreases As uptake by plants via oxidation of As and Se,resulting in decrease of soil As bioavailability and As/Se competitive absorption by *** provides a potential bioremediation strategy for Se biofortification and As immobilization in As-polluted soil.

关键词： Selenium oxidation Selenium biofortification Arsenite-oxidizing bacteria Arsenic immobilization Brassica rapa L

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Bioinformatics Analysis of Disease Resistance Gene PR1 and Its Genetic Transformation in Soybeans and Cultivation of Multi-resistant Materials

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Phyton-International Journal of Experimental Botany 2022年第7期91卷 1445-1464页

作者： huimin cui Shuo Qu Abraham Lamboro Yaolei Jiao Piwu Wang College of Agronomy Jilin Agricultural UniversityChangchun1300118China

In agricultural production,a single insect-resistant and disease-resistant variety can no longer meet the *** this study,the expression vector pCAMBIA-3301-PR1 containing the disease-resistant gene PR1 was constructed by means of genetic engineering,and the PR1 gene was genetically transformed to contain the PR1 gene through the pollen tube *** CryAb-8Like transgenic high-generation T7 receptor soybean,a new material that is resistant to insects and diseases is *** T2 transformed plants,routine PCR detection,Southern Blot hybridization,fluorescence quantitative PCR detection,indoor and outdoor pest resistance identification and indoor disease resistance identification were *** results showed that there were 9 positive plants in the routine PCR test of T2 *** Southern Blot hybridization,both PR1 and CryAb-8Like genes are integrated in soybeans in the form of single *** quantitative PCR showed that the expression levels of PR1 and CryAb-8Like genes are different in different *** average expression levels of PR1 gene in plant roots,stems,and leaves are 2.88,1.54,and 5.26,***-8Like genes are found in roots,stems,and *** average expression levels were 1.36,1.39,and 4.25,*** insectivorous rate of the CryAb-8Like gene in outdoor plants with positive insect resistance identification was 3.78%.The disc partition method was used indoors for pest resistance identification,and the bud length of transformed plants increased *** average mortality rate of untransformed plants in indoor disease resistance identification was as high as 56.66%,and the average mortality rate of plants transformed with PR1 gene was 10.00%,and disease resistance was significantly ***,a new material with resistance to diseases and insects is obtained.

关键词： Soybean PR1 gene CryAb-8Like gene insect resistance disease resistance

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Bioinformatics and Functional Analysis of High Oleic Acid-Related Gene GmSAM22 in Soybean [Glycine max (L.) Merr.]

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Phyton-International Journal of Experimental Botany 2023年第2期92卷 501-519页

作者： Shuo Qu Qi Cai huimin cui Lamboro Abraham Yaolei Jiao Guilong Ma Piwu Wang College of Agronomy Jilin Agricultural UniversityChangchun1300118China College of Plant Protection Agricultural UniversityChangchun1300118China

High yield,high quality,stable yield,adaptability to growth period,and modern mechanization are the basic requirements for crops in the 21st *** oleic acid is a natural unsaturated fatty acid with strong antioxidant properties and *** as a safe fatty acid,it has the ability to successfully prevent cardiovascular and cerebrovascular *** the fatty acid composition of soybean seeds,can not only speed up the breeding process of high-quality high-oil and high-oleic soybeans,but also have important significance in human health,and provide the possibility for the development of soybean oil as a new energy ***,the aim of this study was to analyze the high oleic acid elated gene GmSAM22 in *** this research the soybean oleic acid-related gene GmSAM22 was screened out by Genome-wide association analysis,a 662 bp fragment was acquired by specific PCR amplification,and the pMD18T cloning vector was linked by the use of a seamless cloning *** analysis of the signal peptide prediction,subcellular localization,protein hydrophobicity,transmembrane region analysis,a phosphorylation site,protein secondary and tertiary structure and protein interaction analysis of the protein encoded by the SAM22 gene was carried *** plasmid of the gene editing vector is *** overexpression vector was transformed from pCAMBIA3301 as the base vector,and overexpression vector were *** plants were obtained by genetic transformation by the pollen tube channel *** quantitative PCR was performed on the T2 generation plants to detect the relative expression levels in different *** Blot was used to detect the presence of hybridization *** genes BAR,35S,and NOS in plants were identified by conventional PCR.10 seeds with high and low oleic acid content were chosen for quantitative PCR identification,and finally,the concentration and morphology of soybean fatty acids were

关键词： Soybean SAM22 gene oleic acid cloning CRISPR/Cas9 expression vector

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侧加热腔内的自然对流

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力学进展 2014年第1期44卷 98-136页

作者：徐丰崔会敏北京交通大学土建学院北京100044

开展侧加热腔内自然对流的研究具有重大的环境及工业应用背景.总结侧加热腔内水平温差驱动的自然对流的最新研究进展,并概述相应的流动性质、动力机制和传热特性以及对不同无量纲控制参数的依赖也有重要的科学价值.已取得的研究结果显... 详细信息

开展侧加热腔内自然对流的研究具有重大的环境及工业应用背景.总结侧加热腔内水平温差驱动的自然对流的最新研究进展,并概述相应的流动性质、动力机制和传热特性以及对不同无量纲控制参数的依赖也有重要的科学价值.已取得的研究结果显示突然侧加热的腔内自然对流的发展可包括初始阶段、过渡阶段和定常或准定常阶段.不同发展阶段的流动依赖于瑞利数、普朗特数及腔体的高宽比,且定常或准定常阶段的流态可以是定常层流流动、非定常周期性流动或者湍流流动.此外,回顾了对流流动失稳机制的研究成果以及湍流自然对流方面的新进展.最后,展望了侧加热腔内的自然对流研究的前景.

关键词：自然对流侧加热腔时间依赖

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HOPE:a heterogeneity-oriented parallel execution engine for inference on mobiles

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High Technology Letters 2022年第4期28卷 363-372页

作者： XIA Chunwei ZHAO Jiacheng cui huimin FENG Xiaobing Institute of Computing Technology Chinese Academy of SciencesBeijing 100190P.R.China School of Computer Science and Technology University of Chinese Academy of SciencesBeijing 100190P.R.China

It is significant to efficiently support artificial intelligence(AI)applications on heterogeneous mobile platforms,especially coordinately execute a deep neural network(DNN)model on multiple computing devices of one mobile *** paper proposes HOPE,an end-to-end heterogeneous inference framework running on mobile platforms to distribute the operators in a DNN model to different computing *** problem is formalized into an integer linear programming(ILP)problem and a heuristic algorithm is proposed to determine the near-optimal heterogeneous execution *** experimental results demonstrate that HOPE can reduce up to 36.2%inference latency(with an average of 22.0%)than MOSAIC,22.0%(with an average of 10.2%)than StarPU and 41.8%(with an average of 18.4%)thanμLayer respectively.

关键词： deep neural network(DNN) mobile heterogeneous scheduler parallel computing

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FISH-based “pan” and “core” karyotypes reveal genetic diversification of Roegneria ciliaris

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Journal of Genetics and Genomics 2023年第2期50卷 135-138页

作者： Menghao Cheng Xingyue Li huimin cui Haojie Sun Tianshuo Deng Xinying Song Rongrong Song Tong Wang Zongkuan Wang Haiyan Wang Xiue Wang State Key Laboratory of Crop Genetics and Germplasm Enhancement/Cytogenetics Institute/Collaborative Innovation Center for Modern Crop Production co-sponsored by Province and Ministry(CIC-MCP) Nanjing Agricultural UniversityNanjingJiangsu 210095China

The genus Roegneria was firstly established with Roegneria caucasica *** as the model *** the largest genera in the tribe Triticeae(Poaceae),Roegneria includes 130 species mainly distributed in the north temperate *** are 70 Roegneria species and 22 variants,which are widely distributed in different regions of China(Lei,2018).The diversified morphology of Roegneria species has caused controversy in their *** has been argued whether Roegneria species should be included in the genus Elymus or considered as an independent genus(Lei et al.,2018).The investigation of the cytological and genomic feature would help to clarify the taxonomic placement of Roegneria.

关键词： species Roe temperate

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监察体制改革背景下提高派驻机构监督有效性的思考

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社会科学战线 2019年第9期 211-217页

作者：崔会敏河南大学哲学与公共管理学院

派驻监督在党和国家监督体系中发挥着重要的'前哨'和'探头'作用,监督效用发挥需要依靠'派'的权威和'驻'的优势相结合。在实践中,派驻机构充分发挥监督作用还存在一些困难:派驻机构因监督标准不明确缺乏有力的工作抓手;派驻机构人员配置... 详细信息

派驻监督在党和国家监督体系中发挥着重要的'前哨'和'探头'作用,监督效用发挥需要依靠'派'的权威和'驻'的优势相结合。在实践中,派驻机构充分发挥监督作用还存在一些困难:派驻机构因监督标准不明确缺乏有力的工作抓手;派驻机构人员配置有待于进一步优化;派驻机构干部遭遇'双重边缘化'影响工作积极性;派驻监督的方式和方法有待于进一步创新。在国家监察体制改革背景下,要明确监督标准,解决监督依据问题;优化派驻人员配置,提高派驻干部监督能力;加强纪委统一管理与服务,增强派驻干部监督动力;创新监督方式方法,充分发挥派驻监督效力。

关键词：监督派驻监督监察体制改革监督体系监督标准

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带有变号势函数和Hardy项的临界p-双调和方程弱解的存在性

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上海理工大学学报 2022年第6期44卷 583-587页

作者：崔会敏魏公明上海理工大学理学院上海200093

利用山路引理、集中紧性原理和Hardy不等式,研究了带有变号势函数和Hardy项的临界p-双调和方程弱解的存在性问题。首先验证了山路引理的几何条件,然后证明当0<μ<μ0,山路水平c<2/NS^(N/2p)-μ^p^(*)/(p^(*)-q)G时满足(PS)_(C)条件,最... 详细信息

利用山路引理、集中紧性原理和Hardy不等式,研究了带有变号势函数和Hardy项的临界p-双调和方程弱解的存在性问题。首先验证了山路引理的几何条件,然后证明当0<μ<μ0,山路水平c<2/NS^(N/2p)-μ^p^(*)/(p^(*)-q)G时满足(PS)_(C)条件,最终证明了该类临界p-双调和方程至少存在一个非平凡弱解。

关键词：山路引理集中紧性原理弱解 Hardy不等式

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财政政策与货币政策的组合效应——加强宏观调控应对金融危机

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学术交流 2009年第6期 85-89页

作者：崔惠民安徽财经大学财政与公共管理学院安徽蚌埠233041

财政政策与货币政策是国家对宏观经济进行调控的重要工具,但由于二者调控目标、手段与功能的差异而具有各自的局限性,但同时也形成一定互补性,因此,我们应根据不同的经济形势和调控目标采取不同的政策组合模式。改革开放以来,我们积累... 详细信息

财政政策与货币政策是国家对宏观经济进行调控的重要工具,但由于二者调控目标、手段与功能的差异而具有各自的局限性,但同时也形成一定互补性,因此,我们应根据不同的经济形势和调控目标采取不同的政策组合模式。改革开放以来,我们积累了宏观调控的丰富经验,调控艺术日趋成熟,但全球性金融危机无疑向宏观调控提出了新的挑战,尤其是在我国经济调整期间,我们首先应理顺财政体制和金融体制关系,从制度上保障财政政策与货币政策内在传导机制的通畅,然后在目标导向下优化选择二者的组合效应方式,既要注意防止总体经济的大起大落,又要保证调控的动态性与市场化取向,只有发挥优化组合拳效应才能顺利应对全球金融危机,走出经济调整困境,实现保增长目标。

关键词：财政政策货币政策组合效应双稳健双紧政策双松政策

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广东省高校专利运营能力情报分析——以专利运营能力前10所高校为例

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科技管理研究 2018年第19期38卷 108-116页

作者：崔惠敏华南农业大学图书馆广东广州510642

从专利情报分析的角度,从定量和定性两方面分析广东省高校专利运营能力较强的前10所高校运营情况,包括专利运营总体趋势、专利转让时间特征和地域分布、技术热点分布和被引频次等。研究结果表明,该10所高校的专利运营能力已经走在全国... 详细信息

从专利情报分析的角度,从定量和定性两方面分析广东省高校专利运营能力较强的前10所高校运营情况,包括专利运营总体趋势、专利转让时间特征和地域分布、技术热点分布和被引频次等。研究结果表明,该10所高校的专利运营能力已经走在全国高校前列,但仍需进一步完善专利运用体制建设、培育高价值专利、促进科技成果转化,在广东省乃至全国高校的专利运营中起到引领示范作用。

关键词：专利运营专利申请量专利授权量专利权转让专利许可专利热点技术领域被引频次

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