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Identification of Odor Volatile Compounds and Deodorization ofPaphia undulata Enzymatic Hydrolysate

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Journal of Ocean University of China 2016年第6期15卷 1101-1110页

作者： CHEN Deke CHEN Xin CHEN Hua CAI Bingna WAN Peng ZHU Xiaolian sun Han sun huili PAN Jianyu Key Laboratory of Tropical Marine Bio-resource and Ecology/Guangdong Key Laboratory of Marine Meteria Medica South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 P. R. China University of Chinese Academy of Sciences Beijing 100049 P. R. China Department of Chemistry and Chemical Engineering Foshan University Foshan 528000 P. R. China Ru-tgers University 98 Brett Road Piscataway New Jersey 08854 USA

Unfavorable fishy odour is an inevitable problem in aquatic products. In the present study, headspace solid-phase mi- croextraction gas chromatography mass spectrometry （HS-SPME-GC-MS） analysis of volatiles from untreated samples and three deodorized samples （under the optimal conditions） ofPaphia undulata enzymatic hydrolysate revealed that the compounds contrib- uting to the distinctive odor were 1-octen-3-ol, n-hexanal, n-heptanal, 2,4-heptadienal, and 2,4-decadienal, whereas n-pentanal, n-octanal, n-octanol, benzaldehyde, 2-ethylfuran and 2-pentylfuran were the main contributors to the aromatic flavor. The deodoriz- ing effects of activated carbon （AC） adsorption, yeast extract （YE） masking and tea polyphenol （TP） treatment on a P. undulata en- zymatic hydrolysate were investigated using orthogonal experiments with sensory evaluation as the index. The following optimized deodorization conditions were obtained： AC adsorption （35 mg mL-1, 80℃, 40 rain）, YE masking （7 mgmL l, 45 ℃, 30 min） and TP treatment （0.4mgmL-l, 40℃, 50min）. AC adsorption effectively removed off-flavor volatile aldehydes and ketones. YE masking modified the odor profile by increasing the relative contents of aromatic compounds and decreasing the relative contents of aldehydes and ketones. The TP treatment was not effective in reducing the odor score, but it significantly reduced the relative content of alde- hydes while increasing that of alkanes. It is also notable that TP effectively suppressed trimethylamine （TMA） formation in a P. un- dulate hydrolysate solution for a period of 72 h.

关键词： deodorization off-flavor trimethylamine enzymatic hydrolysate Paphia undulate

Trimethylamine Adsorption Mechanism on Activated Carbon and Removal in Water and Oyster Proteolytic Solution

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Journal of Ocean University of China 2021年第6期20卷 1578-1586页

作者： CHEN Deke WAN Peng CAI Bingna YE Ziqing CHEN Hua CHEN Xin sun huili PAN Jianyu Key Laboratory of Tropical Marine Bio-Resource and Ecology/Guangdong Key Laboratory of Marine Meteria Medica South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China Innovation Academy of South China Sea Ecology and Environmental Engineering(ISEE) Chinese Academy of SciencesGuangzhou 510000China School of Environment and Chemical Engineering Foshan UniversityFoshan 528000China University of Chinese Academy of Sciences Beijing 100049China

In this study,seven coal-based activated carbons(ACs)were adopted to remove trimethylamine(TMA)in an aqueous solution as environmentally friendly and harmless *** results showed that columnar AC(CAC)had a clear scale and honeycomb structures with few fragments and micropores,contributing to superior TMA removal capacity compared to granular AC(GAC)(71.67%for 6.0 mm CAC and 69.92%for 40–60 mesh GAC).In addition,the process of adsorption was accompanied by desorption,and the recommended absorbed time was 120–180 *** short time to achieve equilibrium indicated that adsorption was kinetically controlled,and pseudo-second-order kinetics was more appropriate than pseudo-first-order kinetics in explaining the adsorption mechanism in both water and oyster enzymatic *** intraparticle diffusion model presented that the adsorption processes could be divided into three steps for GAC and two steps for *** adsorption processes were consistent with the Freundlich model,indicating the existence of physisorption and chemisorption as multilayer *** results indicated that AC,especially CAC,has great potential for TMA elimination in aquatic product processing.

关键词： trimethylamine activated carbon adsorption mechanism oyster proteolytic solution

Structural characterization of oligosaccharide from Spirulina platensis and its effect on the faecal microbiota in vitro

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Food Science and Human Wellness 2022年第1期11卷 109-118页

作者： Bingna Cai Xiangxi Yi Qian Han Jianyu Pan Hua Chen huili sun Peng Wan Key Laboratory of Tropical Marine Bio-Resources and Ecology Guangdong Key Laboratory of Marine Materia MedicaSouth China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou510301 GuangdongChina Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou511458 GuangdongChina School of Pharmacy Guangxi University of Chinese MedicineNanning530200 GuangxiChina Innovation Academy of South China Sea Ecology and Environmental Engineering(ISEE) Chinese Academy of SciencesGuangzhou510000 GuangdongChina

In the present study,an oligosaccharide SPO-1 from Spirulina platensis was prepared by glycosidase from a marine *** prebiotic activity of SPO-1 on the growth of Lactobacillus paracasei and Bifidobacterium animalis,and its effect on human gut microbiota were examined in *** molecular weight of the tetrasaccharide SPO-1 was 650.2 Da,and it was mainly composed of glucose with α-type glycosidic *** prebiotic activity score of SPO-1 was the highest for the growth of probiotic strains *** and ***,as fermentation proceeded,SPO-1 was gradually degraded and utilized by intestinal *** results showed that after treatment with SPO-1,carbohydrate consumption and short-chain fatty acids levels were increased,especially those of i-butyric and i-valeric ***,SPO-1 significantly promoted the abundance,diversity and composition of gut microbiota,especially stimulating the growth of Bacteroides,Escherichia-Shigella and *** addition,the change in intestinal microbiota function predicted by phylogenetic investigation of communities by reconstruction of unobserved states(PICRUSt)after treatment with SPO-1 is mainly related to the terms“carbohydrate metabolism”and“amino acid metabolism”.These results suggest that SPO-1 is a potential oligosaccharide in regulation of intestinal microbiota.

关键词： Oligosaccharide Spirulina platensis Gut microbiota In vitro fermentation

Ultrasonic Extraction of Polysaccharides from Laminaria japonica and Their Antioxidative and Glycosidase Inhibitory Activities

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Journal of Ocean University of China 2015年第4期14卷 651-662页

作者： WAN Peng YANG Xiaoman CAI Bingna CHEN Hua sun huili CHEN Deke PAN Jianyu CAS Key Laboratory of Tropical Marine Bio-resources and Ecology Guangdong Key Laboratory of Marine Materia Medica South China Sea Institute of Oceanology Chinese Academy of Sciences University of Chinese Academy of Sciences Ocean University of China

In the present study, ultrasonic extraction technique （UET） is used to improve the yield of polysaccharides from Lami- naria japonica （LJPs）. And their antioxidative as well as glycosidase inhibitory activities are investigated. Box-Behnken design （BBD） combined with response surface methodology （RSM） is applied to optimize ultrasonic extraction for polysaccharides. The optimized conditions are obtained as extraction time at 54 min, ultrasonic power at 1050 W, extraction temperature at 80℃ and ratio of material to solvent at 1：50 （g mL-1）. Under these optimal ultrasonic extraction conditions, an actual experimental yield （5.75% ＋ 0.3%） is close to the predicted result （5.67%） with no significant difference （P〉0.05）. Vitro antioxidative and glycosidase inhibitory activities tests indicate that the crude polysaccharides （LJP） and two major ethanol precipitated fractions （LJP1 and LJP2） are in a concentration-dependent manner. LJP2 （30%-60% ethanol precipitated polysaccharides） possesses the strongest α-glucosidase in- hibitory activity and moderate scavenging activity against hydroxyl radicals （66.09% ±2.19%, 3.0 mg mL-l）. Also, the inhibitory activity against a-glucosidase （59.08% ± 3.79%, 5.0 mg mL-1） is close to that of acarbose （63.99% ± 3.27%, 5.0 mg mL-l）. LJP 1 （30% ethanol precipitated polysaccharides） exhibits the strongest scavenging activity against hydroxyl radicals （99.80%q-0.00%, 3.0mg mL-1） and moderate a-glucosidase inhibitory activity （47.76%± 1.92%, 5.0 mgmL-1）. LJP shows the most remarkable DPPH scav- enging activity （66.20%±0.11%, 5.0mgmL-1） but weakest a-glucosidase inhibitory activity （37.77%±1.30%, 5.0mgmL-1）. How- ever, all these LJPs exert weak inhibitory effects against a-amylase. These results show that UET is an effective method for extract- ing bioactive polysaccharides from seaweed materials. LJP 1 and LJP2 can be developed as a potential ingredient in hypoglycemic agents or functional food for the management of diabetes. This study provi

关键词： polysaccharides Laminariajaponica ultrasonic extraction antioxidative α-glucosidase a-amylase

Production of reactive oxygen species by PuRBOHF is critical for stone cell development in pear fruit

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Horticulture Research 2021年第1期8卷 3438-3448页

作者： Xiaoqian Wang Siqi Liu huili sun Chunyan Liu Xinyue Li Yang Liu Deguo Lyu Guodong Du College of Horticulture Shenyang Agricultural UniversityShenyang 110866P.R.China

The production of reactive oxygen species(ROS)by NADPH oxidase,which is also referred to as respiratory burst oxidase homolog(RBOH),affects several processes in ***,the role of RBOHs in cell wall lignification is not well *** this study,we show that PuRBOHF,an RBOH isoform,plays an important role in secondary wall formation in pear stone *** were closely associated with lignin deposition and stone cell formation according to microscopy *** addition,according to the results of an in situ hybridization analysis,the stage-specific expression of PuRBOHF was higher in stone cells than in cells of other flesh *** of RBOH activity suppressed ROS accumulation and stone cell lignification in pear ***,transient overexpression of PuRBOHF caused significant changes in the amount of ROS and lignin that accumulated in pear fruit and flesh *** further showed that PuMYB169 regulates PuRBOHF expression,while PuRBOHF-derived ROS induces the transcription of PuPOD2 and *** findings of this study indicate that PuRBOHF-mediated ROS production,which is regulated by a lignin-related transcriptional network,is essential for monolignol polymerization and stone cell formation in pear fruit.

关键词： critical RBO transient

Culture-free identification of fast-growing cyanobacteria cells by Raman-activated gravity-driven encapsulation and sequencing

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Synthetic and Systems Biotechnology 2023年第4期8卷 708-715页

作者： Jinyu Cui Rongze Chen huili sun Yingyi Xue Zhidian Diao Jingyun Song Xiaohang Wang Jia Zhang Chen Wang Bo Ma Jian Xu Guodong Luan Xuefeng Lu Key Laboratory of Biofuels Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesNo.189 Songling RoadQingdao266101China Shandong Energy Institute Qingdao266101China Qingdao New Energy Shandong Laboratory Qingdao266101China College of Life Science University of Chinese Academy of SciencesBeijingChina Dalian National Laboratory for Clean Energy Dalian116023China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdaoChina

By directly converting solar energy and carbon dioxide into biobased products,cyanobacteria are promising chassis for photosynthetic *** make cyanobacterial photosynthetic biosynthesis technology economically feasible on industrial scales,exploring and engineering cyanobacterial chassis and cell factories with fast growth rates and carbon fixation activities facing environmental stresses are of great *** simplify and accelerate the screening for fast-growing cyanobacteria strains,a method called Individual Cyanobacteria Vitality Tests and Screening(iCyanVS)was *** show that the ^(13)C incorporation ratio of carotenoids can be used to measure differences in cell growth and carbon fixation rates in individual cyanobacterial cells of distinct genotypes that differ in growth rates in bulk cultivations,thus greatly accelerating the process screening for fastest-growing *** feasibility of this approach is further demonstrated by phenotypically and then genotypically identifying individual cyanobacterial cells with higher salt tolerance from an artificial mutant library via Raman-activated gravity-driven encapsulation and ***,this method should find broad applications in growth rate or carbon intake rate based screening of cyanobacteria and other photosynthetic cell factories.

关键词： Cyanobacteria Single-cell Raman microspectroscopy Stable isotope probing Carotenoids Identification

Study on preparation of chito-oligosaccharide by cellulase hydrolysis

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Study on preparation of chito-oligosaccharide by cellulase h...

Multiple routes toward engineering efficient cyanobacterial photosynthetic biomanufacturing technologies

第三届材料科学应用与能源材料国际研讨会

作者： Xin Chen Gaofeng Li Pengcheng Dong huili sun School of Environment and Chemical Engineering Foshan University South China Sea Institute of Chinese Academy of Sciences

By using cellulase enzymatic hydrolysis preparation to obtain low molecular weight chito-oligosaccharide. The conditions for the hydrolysis of chitosan by cellulase were studied by single factor experiment. The effects of temperature, pH value, reaction time and enzyme dosage on the decomposition efficiency of chitosan were *** conclusions from the experiment are as follows: the optimum conditions for the preparation of chito-oligosaccharide by cellulase hydrolysis are reaction temperature of 50 ℃, pH of 4.8, enzymatic hydrolysis time of 3.5 h, the enzyme dosage of 50 U/mg, in this case its economic benefits is greatest.

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Green Carbon 2023年第2期1卷 210-226页

作者： Jinyu Cui huili sun Rongze Chen Jiahui sun Guanlan Mo Guodong Luan Xuefeng Lu Key Laboratory of Biofuels Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesNo.189 Songling RoadQingdao 266101China Shandong Energy Institute Qingdao 266101China Qingdao New Energy Shandong Laboratory Qingdao 266101China College of Life Science University of Chinese Academy of SciencesBeijing 100049China Dalian National Laboratory for Clean Energy Dalian 116023China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China

Developing efficient CO_(2)utilization technologies can alleviate the urgent pressure on energy and the ***,these technologies are crucial for achieving the goal of net zero *** are photoautotrophic microorganisms that are the main sources of primary productivity in the ***,the only prokaryotic microalgae,have also been considered as promising chassis for photosynthetic biosynthesis,directly converting solar energy and CO_(2)into various bio-based *** technological route is called photosynthetic biomanufacturing,and is advantageous to simultaneous carbon fixation and clean *** review focuses on development mode,application and suggests trends related to the further development of photosynthetic *** regard to the link between photosynthetic CO_(2)fixation and the production of desired metabolites,we summarized and compared three widely adopted strategies.“Screening to find”,screening a large number of high-quality cyanobacterial resources and analyzing their intracellular metabolites are of significance for screening novel cyanobacterial species with high-value chemicals and properties of industrial relevance.“Engineering to modify”,the emergence and application of synthetic biological tools and metabolic engineering strategies have enhanced the ability to modify different cyanobacterial species to reshape more carbon to flow toward synthetic tailored chemicals.“Stressing to activate”,through special culture conditions and strategies,combined with omics analysis techniques,silent metabolic pathways and functional modules are activated to induce the accumulation of high-value *** review provides valid and updated information to facilitate the development of photosynthetic biosynthesis route with carbon fixation and clean production,providing specific feasible solutions for net zero emissions.

关键词： Cyanobacteria Photosynthetic biomanufacturing Net zero emissions Metabolic engineering Stressing

Orthogonal experimental study on preparation of chitosan oligosaccharide based on cellulase hydrolysis

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Orthogonal experimental study on preparation of chitosan oli...