Study on preparation of chito-oligosaccharide by cellulase hydrolysis
作者单位：School of Environment and Chemical Engineering Foshan University South China Sea Institute of Chinese Academy of Sciences
会议名称：《第三届材料科学应用与能源材料国际研讨会》
会议日期：2020年
学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术]
基 金：supported by Water Pollution Control Engineering Technology Research Center of Guangdong, 2017 Guangdong Science and Technology Project Environmental Pollution Catalytic Treatment Engineering Technology Research Center of Foshan, 2017 Foshan Scienceand Technology Project(2017GA10159)
摘 要：By using cellulase enzymatic hydrolysis preparation to obtain low molecular weight chito-oligosaccharide. The conditions for the hydrolysis of chitosan by cellulase were studied by single factor experiment. The effects of temperature, pH value, reaction time and enzyme dosage on the decomposition efficiency of chitosan were *** conclusions from the experiment are as follows: the optimum conditions for the preparation of chito-oligosaccharide by cellulase hydrolysis are reaction temperature of 50 ℃, pH of 4.8, enzymatic hydrolysis time of 3.5 h, the enzyme dosage of 50 U/mg, in this case its economic benefits is greatest.