检索结果-南通市图书馆

TIGAR mediates the inhibitory role of hypoxia on ROS production and apoptosis in rat nucleus pulposus cells

在线全文

学校读者我要写书评

暂无评论

TIGAR mediates the inhibitory role of hypoxia on ROS product...

第二十四届中国中西医结合骨伤科学术年会

作者： Li-Bo Jiang hui-ren wang Xi-Lei Li Jian Dong Department of Orthopedic Surgery Zhongshan Hospital Fudan University

Objective Hypoxia has been shown to inhibit reactive oxygen species(ROS)production in nucleus pulposus(NP)*** TP53-induced glycolysis and apoptosis regulator(TIGAR)has been reported to suppress oxidative *** sought to explore the role of TIGAR in the effect of hypoxia on ROS production and *** An intervertebral disc degeneration(IDD)model of Sprague-Dawley(SD)rat caudal spine was established by stabbing their intervertebral *** expression was detected by immunohistochemistry in human and SD rat NP tissues of degenerated *** primary NP cells treated with hypoxia and cobalt chloride(CoCl)were analyzed by western blotting for TIGAR *** TIGAR inhibition with TIGAR siRNA transfection,apoptosis percentage,mitochondrial and total intracellular ROS levels were *** was used to further check the effects of TIGAR on oxidative ***,NADPH/NADPand GSH/GSSH ratio were examined after TIGAR silencing under hypoxic conditions and after HO *** degree-dependent increase in TIGAR expression was observed in human and rat degenerated NP *** and hypoxia-inducer CoClenhanced TIGAR and P53 expressions in rat NP *** inhibition reversed the inhibitory effects of hypoxia on intracellular and mitochondrial ROS production,as well as apoptosis ***,TIGAR inhibition aggravated HO-induced ROS *** addition,TIGAR increased NADPH/NADPand GSH/GSSH ratio in NP *** hese results suggested that TIGAR appears to mediate the protective role of hypoxia on ROS production and apoptosis percentage by enhancing NADPH/NADP+and GSH/GSSH ratio.

关键词： ntervertebral disc degeneration Nucleus pulposus Hypoxia Reactive oxygen species TP53-induced glycolysis and apoptosis regulator

Degradation and osteogenic potential of a novel PLA/nano-sizedβ-TCPscaffolds

在线全文

cnki会议

学校读者我要写书评

暂无评论

Degradation and osteogenic potential of a novel PLA/nano-siz...

第三届全国中西医结合骨科微创学术交流会

作者： Lu Cao Shao-Jun wang Ping-Guo Duan Feng-Lai Yuan hui-ren wang Jian Dong Department of Orthopaedic Surgery Zhongshan HospitalFudan University The third hospital affiliated to Nantong University State Key Laboratory of Molecular Engineering of Polymers Fudan University

The effect of increasing nano-sizedβ-tricalcium phosphate(β-TCP) content on the biological performance of polylactic acid(PLA) composite scaffold was investigated by in vitro degradation and in vivo model of heterotopic bone ***-sizedβ-TCP was prepared by wet grinding method,PLA/nano-sizedβ-TCP scaffold with increasingβ-TCP(0-50wt%)contents were fabricated by freeze-drying *** degradation behavior was carried out by determining the microstructure, the change of pH,weight and comprehensive strength in 26 weeks of *** study in vivo was performed by cutting the composite scaffold contain0,10,30,50%β-TCP and pureβ-TCP into blocks(5mm×5mm×3mm),and then implanting into subcutaneous tissue of back in rabbits after loading recombinant human bone morphine protein-2(rhBMP-2),each rabbit received 4 *** in vitro results indicated a marked pH buffering effect as nano-sizedβ-TCP filler increased, however,comprehensive strength of composite scaffolds decreased *** the other hand the findings in vivo demonstrated that increasing percentage of nano-sizedβ-TCP to PLA scaffolds enhanced their osteoconductivity. Composite scaffold containing 50%β-TCP possessed a similar biological performance to pureβ-TCP in terms of osteoconductivity,but scaffold containing 30%β-TCP exhibited better mechanical properties,which can meet the demand of bone repair in load-bearing *** supports the potential future application of PLA/30n-β-TCP scaffold as ideal scaffold for bone tissue engineering.

关键词： bone tissue engineering polylactide tricalcium phosphate nano-sized porous scaffold

Degradation and osteogenic potential of a novel PLA/nano-sized β-TCPscaffolds

在线全文

cnki会议

学校读者我要写书评

暂无评论

Degradation and osteogenic potential of a novel PLA/nano-siz...

第二十届全国中西医结合骨伤科学术研讨会、第二届中国医师协会中西医结合医师分会骨伤科学术年会、第十九届浙江省中西医结合骨伤科专业委员会学术年会

The effect of increasing nano-sized P -tricalcium phosphate(P -TCP) content on the biological performance of polylactic acid(PLA) composite scaffold was investigated by in vitro degradation and in vivo model of heterotopic bone ***-sized P -TCP was prepared by wet grinding method,PLA/nano-sizedβ-TCP scaffold with increasingβ-TCP(0-50wt%) contents were fabricated by freeze-drying method. The degradation behavior was carried out by determining the microstructure,the change of pH,weight and comprehensive strength in 26 weeks of *** study in vivo was performed by cutting the composite scaffold contain0,10,30,50%β-TCP and pureβ-TCP into blocks(5mm×5mm×3mm),and then implanting into subcutaneous tissue of back in rabbits after loading recombinant human bone morphine protein-2 (rhBMP-2),each rabbit received 4 *** in vitro results indicated a marked pH buffering effect as nano-sized 0 -TCP filler increased,however,comprehensive strength of composite scaffolds decreased *** the other hand the findings in vivo demonstrated that increasing percentage of nano-sizedβ-TCP to PLA scaffolds enhanced their *** scaffold containing 50%β-TCP possessed a similar biological performance to pureβ-TCP in terms of osteoconductivity,but scaffold containing 30%β-TCP exhibited better mechanical properties,which can meet the demand of bone repair in load-bearing *** supports the potential future application of PLA/30n-β-TCP scaffold as ideal scaffold for bone tissue engineering.

关键词： bone tissue engineering polylactide tricalcium phosphate nano-sized porous scaffold

Minimum five-year follow-up of multi-segmental lumbar disc degenerationtreated with discectomy andWallis

在线全文

cnki会议

学校读者我要写书评

暂无评论

Minimum five-year follow-up of multi-segmental lumbar disc d...

第二十一届全国中西医结合骨伤科学术研讨会暨骨伤科分会换届大会

作者： Yun-qi Jiang Wu Che hui-ren wang Ruo-yu Li Hong Lin Jian Dong Department of Orthopaedic Surgery Zhongshan HospitalFudan University

Study Design Retrospective *** To evaluate the clinical effects and radiological findings of Wallis for the treatment of multi-segmental lumbar disc degeneration after a minimum five-year *** of Background Data Lumbar fusion for lumbar disc degeneration is a common *** surgery,many complicationsmight arise;therefore,differentmethodsfordynamicfixationhavebeen *** has been designed to improve segmental stability while preserving intervertebral mobility after *** could reduce the incidence of adjacent segment *** A total of 26 adult patients with complete data underwent primary discectomy followed by fixation of the segment with Wallis between December 2007 and August *** males and 14femaleswithanagerangeof43to 56years(average47.6)*** visual analogue scale(VAS)for low back and leg pain,Oswestry Disability Index(ODI),foraminal height(FH),anterior disc height(aDH)and posterior disc height(pDH),range of motion(ROM)and Pfarmanngradewereobtainedandcomparedbefore andafter *** The VAS and ODIdecreased postoperatively with statistical significance(p0.05).The postoperative FH and pDH increased with statistical significance compared with the preoperative levels(P0.05).There were no statistically significant changes in the range ofmotion andthe Pfurmann grade atthe instrumented level and the cephalad-adjacent segment(p>0.05).In our study,no patient underwent further surgery because of a re-prolapse or progression of degeneration ofthe index level and adjacent segment *** Wallis was a good alternative for treating multi-segmental lumbar disc degeneration,and it offered significantandlo

关键词：

EZH2-dependent myelination following sciatic nerve injury

在线全文

cnki会议

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第8期20卷 2382-2394页

作者： hui Zhu Li Mu Xi Xu Tianyi Huang Ying wang Siyuan Xu Yiting wang Wencong wang Zhiping wang Hongkui wang Chengbin Xue Research Center of Clinical Medicine Affiliated Hospital of Nantong UniversityKey Laboratory of Neuroregeneration of Jiangsu and Ministry of EducationCo-innovation Center of NeuroregenerationNMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology ProductsNantong UniversityNantongJiangsu ProvinceChina Medical School of Nantong University NantongJiangsu ProvinceChina Department of Neurology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Rehabilitation Medicine Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Critical Care Medicine Nantong Fourth People’s HospitalNantongJiangsu ProvinceChina

Demyelination and remyelination have been major focal points in the study of peripheral nerve regeneration following peripheral nerve ***,the gene regulatory network of regenerated myelin differs from that of native *** of enhancer of zeste homolog 2(EZH2)hinders the differentiation,maturation,and myelination of Schwann cells in *** further determine the role of EZH2 in myelination and recovery post-peripheral nerve injury,conditional knockout mice lacking Ezh2 in Schwann cells(Ezh2^(fl/fl);Dhh-Cre and Ezh2^(fl/fl);Mpz-Cre)were *** results show that a significant proportion of axons in the sciatic nerve of Ezh2-depleted mice remain *** highlights the crucial role of Ezh2 in initiating Schwann cell ***,we observed that 21 days after inducing a sciatic nerve crush injury in these mice,most axons had remyelinated at the injury site in the control nerve,while Ezh2^(fl/fl);Mpz-Cre mice had significantly fewer remyelinated axons compared with their wild-type *** suggests that the absence of Ezh2 in Schwann cells impairs myelin formation and *** conclusion,EZH2 has emerged as a pivotal regulatory factor in the process of demyelination and myelin regeneration following peripheral nerve *** EZH2 activity during these processes may offer a promising therapeutic target for the treatment of peripheral nerve injuries.

关键词： demyelination EZH2 myelination peripheral nerve injury PRC2 remyelination Schwann cells sciatic nerve crush sciatic nerve transection

Unraveling brain aging through the lens of oral microbiota

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第7期20卷 1930-1943页

作者： Qinchao Hu Si wang Weiqi Zhang Jing Qu Guang-hui Liu State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Hospital of Stomatology Guanghua School of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders Xuanwu HospitalCapital Medical UniversityBeijingChina Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijingChina CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijingChina University of Chinese Academy of Sciences BeijingChina Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijingChina Aging Biomarker Consortium BeijingChina State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Beijing Institute for Stem Cell and Regenerative Medicine BeijingChina

The oral cavity is a complex physiological community encompassing a wide range of *** of oral microbiota can lead to various oral infectious diseases,such as periodontitis and tooth decay,and even affect systemic health,including brain aging and neurodegenerative *** studies have highlighted how oral microbes might be involved in brain aging and neurodegeneration,indicating potential avenues for intervention *** this review,we summarize clinical evidence demonstrating a link between oral microbes/oral infectious diseases and brain aging/neurodegenerative diseases,and dissect potential mechanisms by which oral microbes contribute to brain aging and *** also highlight advances in therapeutic development grounded in the realm of oral microbes,with the goal of advancing brain health and promoting healthy aging.

关键词： Alzheimer's disease brain aging multiple sclerosis neurodegeneration neurodegenerative diseases oral microbiota Parkinson's disease periodontitis bacteria Porphyromonas gingivalis

The biomechanical stability of a novel totally bioabsorbable self-retaining PLA/nano-sizedβ-TCP cervical spine interbody fusion device in single level ACDF models

在线全文

学校读者我要写书评

暂无评论

The biomechanical stability of a novel totally bioabsorbable...

作者： Lu Cao Ping-Guo Duan Xi-Lei Li hui-ren wang Wu Che Jian Dong Feng-Lai Yuan Ming-Dong Zhao Dong-Sheng Zhang Department of Orthopedic Surgery Zhongshan HospitalFudan University State Key Laboratory of Molecular Engineering of Polymers Fudan University Affiliated Third Hospital ofNantong University Department of Orthopedic Surgery Jinshan HospitalFudan University Department of Mechanics Shanghai University

PURPOSE The purpose of this study was to compare the biomechanical stability provided by a novel PLA/ nano-sizedβ-TCP bioabsorbable self-retaining cervical fusion cage(BCFC) device,and compare it with tricortical itiac crest bone graft and two types of PEEK cages according to their shape and anchoring design,and furtherly to compare with tricortical iliac crest bone graft plus anterior cervical plate(ACP). METHODS Quasistatic nonconstraining torques(maximum 1.5NM) induced flexion,extension, lateral bending(±1.5NM),and axial rotation(±1.5NM) on 32 sheep cervical spines(C2-C5).The motion segment C3-C4 was firstly tested intact,the following groups were tested after complete discectomy: autologous tricortical iliac crest bone graft,Wego cage,Solis cage,and ***,the group of autologous bone graft was evaluated with *** mean range of motion(ROM) was calculated from the load-displacement curves. RESULTS No significant difference in ROM between two types of PEEK cages and BCFC could be observed in flexion and extension,BCFC device significantly decreased ROM in lateral bending and axial rotation compared with all *** significant difference in ROM between BCFC and bone graft plus ACP could be determined in lateral bending and axial rotation. CONCLUSIONS BCFC device showed comparable stability to autologous tricortical iliac crest bone graft and PEEK cages in single level ACDF models,it may be a viable alternative to current PEEK cages and anterior plate devices.

关键词： Biomechanics Cervical spine Cages Bioabsorbable Sheep

The biomechanical stability of a novel totally bioabsorbable self-retaining PLA/nano-sizedβ-TCP cervical spine interbody fusion device in single level ACDF models

在线全文

cnki会议

学校读者我要写书评

暂无评论

The biomechanical stability of a novel totally bioabsorbable...

第三届全国中西医结合骨科微创学术交流会

作者： Lu Cao Ping-Guo Duan Xi-Lei Li hui-ren wang Feng-Lai Yuan Ming-Dong Zhao Wu Che Dong-Sheng Zhang Jian Dong Department of Orthopedic Surgery Zhongshan HospitalFudan University State Key Laboratory of Molecular Engineering of Polymers Fudan University Affiliated Third Hospital of Nantong University Department of Orthopedic Surgery Jinshan HospitalFudan University

Purpose:The purpose of this study was to compare the biomechanical stability provided by a novel PLA/nano-sizedβ-TCP bioabsorbable self-retaining cervical fusion cage(BCFC) device,and compare it with tricortical iliac crest bone graft and two types of PEEK cages according to their shape and anchoring design,and furtherly to compare with tricortical iliac crest bone graft plus anterior cervical plate(ACP). Methods:Quasistatic nonconstraining torques(maximum 1.5NM) induced flexion,extension,lateral bending(±1.5 NM),and axial rotation(±1.5 NM) on 32 sheep cervical spines(C2-C5).The motion segment C3-C4 was firstly tested intact,the following groups were tested after complete discectomy:autologous tricortical iliac crest bone graft, Wego cage,Solis cage,and ***,the group of autologous bone graft was evaluated with *** mean range of motion(ROM) was calculated from the loaddisplacement curves. Results:No significant difference in ROM between two types of PEEK cages and BCFC could be observed in flexion and extension,BCFC device significandy decreased ROM in lateral bending and axial rotation compared with all *** significant difference in ROM between BCFC and bone graft plus ACP could be determined in lateral bending and axial rotation. Conclusions:BCFC device showed comparable stability to autologous tricortical iliac crest bone graft and PEEK cages in single level ACDF models,it may be a viable alternative to current PEEK cages and anterior plate devices.

关键词： Biomechanics Cervical spine Cages Bioabsorbable Sheep

在线全文

cnki会议

学校读者我要写书评

暂无评论

发达国家循环经济发展模式分析及借鉴

特区经济 2009年第7期246卷 84-85页

作者：苗绘王仁波李海申河北金融学院河北保定071051

循环经济是我国实现可持续发展的战略选择,但目前我国循环经济发展存在诸多制约因素。本文借鉴西方发达国家发展循环经济的经验,提出了推进我国循环经济发展的政策建议。

关键词：循环经济可持续发展