Degradation and osteogenic potential of a novel PLA/nano-sizedβ-TCPscaffolds
作者单位：Department of Orthopaedic SurgeryZhongshan HospitalFudan University The third hospital affiliated to Nantong University State Key Laboratory of Molecular Engineering of PolymersFudan University
会议名称：《第三届全国中西医结合骨科微创学术交流会》
会议日期：2013年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学]
关 键 词：bone tissue engineering polylactide tricalcium phosphate nano-sized porous scaffold
摘 要：The effect of increasing nano-sizedβ-tricalcium phosphate(β-TCP) content on the biological performance of polylactic acid(PLA) composite scaffold was investigated by in vitro degradation and in vivo model of heterotopic bone ***-sizedβ-TCP was prepared by wet grinding method,PLA/nano-sizedβ-TCP scaffold with increasingβ-TCP(0-50wt%)contents were fabricated by freeze-drying *** degradation behavior was carried out by determining the microstructure, the change of pH,weight and comprehensive strength in 26 weeks of *** study in vivo was performed by cutting the composite scaffold contain0,10,30,50%β-TCP and pureβ-TCP into blocks(5mm×5mm×3mm),and then implanting into subcutaneous tissue of back in rabbits after loading recombinant human bone morphine protein-2(rhBMP-2),each rabbit received 4 *** in vitro results indicated a marked pH buffering effect as nano-sizedβ-TCP filler increased, however,comprehensive strength of composite scaffolds decreased *** the other hand the findings in vivo demonstrated that increasing percentage of nano-sizedβ-TCP to PLA scaffolds enhanced their osteoconductivity. Composite scaffold containing 50%β-TCP possessed a similar biological performance to pureβ-TCP in terms of osteoconductivity,but scaffold containing 30%β-TCP exhibited better mechanical properties,which can meet the demand of bone repair in load-bearing *** supports the potential future application of PLA/30n-β-TCP scaffold as ideal scaffold for bone tissue engineering.