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Asian Journal of Andrology 2011年第6期13卷 862-866页

作者： Zhi-Hong Niu hui-juan shi hui-Qin Zhang Ai-Jun Zhang Yi-juan Sun Yun Feng IVF Unit Department of Obstetrics and Gynecology Ruijin Hospital Affiliated to Shanghai Jiao Tong University Shanghai 200025 China Shanghai Institute of Planned Parenthood Research Shanghai 200032 China

The aim of this study was to investigate whether the sperm chromatin structure assay （SCSA） results after swim-up are related to fertilization rates, embryo quality and pregnancy rates following in vitrofertilization （IVF）. A total of 223 couples undergoing IVF in our hospital from October 2008 to September 2009 were included in this study. Data on the IVF process and sperm chromatin structure assay results were collected. Fertilization rate, embryo quality and IVF success rates of different DNA fragmentation index （DFI） subgroups and high DNA stainability （HDS） subgroups were compared. There were no significant differences in fertilization rate, clinical pregnancy or delivery rates between the DFI and HDS subgroups. However, the group with abnormal DFI had a lower good embryo rate. So, we concluded that the SCSA variables, either DFI or HDS after swim-up preparation, were not valuable in predicting fertilization failure or pregnancy rate, but an abnormal DFI meant a lower good embryo rate following IVF.

关键词： embryo fertilization in-vitro fertilization sperm chromatin structure assay

Prevalent false positives of azoospermia factor a （AZFa） microdeletions caused by single-nucleotide polymorphism rs72609647 in the sY84 screening of male infertility

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Asian Journal of Andrology 2011年第6期13卷 877-880页

作者： Qing Wu Guo-Wu Chen Tao-Fei Yan hui Wang Yu-Ling Liu Zheng Li shi-Wei Duan Fei Sun Yun Feng hui-juan shi Reproductive Medical Center Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200025 China Shanghai Jiai Genetics and IVF Institute Center of China-USA Shanghai 200011 China Department of Obstetrics and Gynecology Second Military Medical University Shanghai 200433 China China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices Shanghai Institute of Planned Parenthood Research Shanghai 200032 China Shanghai Human Sperm Bank Shanghai Institute of Andrology Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200001 China School of Medicine Ningbo University Ningbo 315033 China Laboratory for Reproductive Biology School of Life Science University of Science and Technology of China Hefei 230026 China

Multiplex polymerase chain reaction （PCR） has been widely used to detect Y-chromosome micredeletions, which is one of the major causes of male infertility. Both the European Academy of Andrology （EAA） and the European Molecular Genetics Quality Network （EMQN） have recommended the use of sY84 and sY86 markers for the detection of azoospermia factor a （AZFa） microdeletion during DNA testing for male infertility. In this study, a large-scale analysis of AZF microdeletion in a total of 630 Chinese males, including healthy semen donors （n=200）, infertile males with normal sperm count （n=226） and patients with either nonobstructive azoospermia or severe oligozoospermia （n=204）, was performed. A series of nine sequence-tagged site （STS） markers from the AZF region of the Y chromosome was used to detect microdeletions. All primers were designed based on the recommendations of the National Center for Biotechnology Information. An unusually high incidence （73/630, 11.6%） of sY84-absent but sY86-present genotypes was observed in the AZFa microdeletion screening. Sequencing the sY84-flanking region revealed a total of 73 patients with sY84-absent but sY86-present genotypes have a T-to-G transversion at the fifth base from the 5＇ end of the reverse sY84 primer. These prevalent false positives, which were not only observed in infertile men, but also observed in donors, resulted from a single-nucleotide polymorphism （SNP） named rs72609647 in the targeting sequence of the reverse sY84 primer. Our study suggests that a pre-screening of existence of rs72609647 polymorphism can prevent the frequent false positive results of AZFa microdeletions detection in the infertile Chinese males. Given the SNP rs72609647 was recently found in a deep sequencing of a Chinese individual, the current EAA and EMQN standards may need to be scrutinized among different populations to avoid the potential genetic variations in the primer binding sequences.

关键词： male infertility multiplex polymerase chain reaction rs72609647 single-nucleotide polymorphism sY84 Y-chromosomemicrodeletion

Malignant adenomyoepithelioma of the breast:Two case reports and review of the literature

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World Journal of Clinical Cases 2021年第31期9卷 9549-9556页

作者： Duan-Yang Zhai Tian-Tian Zhen Xiao-Ling Zhang Jia Luo hui-juan shi Ya-Wei shi Nan Shao The Breast Disease Center The First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510000Guangdong ProvinceChina Department of Pathology The First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510000Guangdong ProvinceChina Department of Radiology The First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510000Guangdong ProvinceChina Department of Medical Ultrasonics The First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510000Guangdong ProvinceChina

BACKGROUND Malignant adenomyoepithelioma(AME)of the breast is a rare tumor in which malignancy can arise from either epithelial or myoepithelial components,or from both cell *** incidence and prognosis of malignant AME of the breast are difficult to assess due to its ***,the optimal treatment for this disease is still *** SUMMARY We present two middle-aged women(48 and 56 years old)with malignant AME of the *** needle biopsy was performed before ***,breast adenoma and malignant tumors were *** preoperative diagnosis of malignant AME of the breast is still challenging for pathologists and *** patients underwent mastectomy and sentinel lymph node biopsy,both of which were negative,followed by adjuvant *** The follow-up duration of the two patients was two years and four months,*** signs of relapse or metastasis have been observed thus far.

关键词： Breast Malignant adenomyoepithelioma Imaging Immunohistochemistry Literature review Case report

Prohibitin(PHB)interacts with AKT in mitochondria to coordinately modulate sperm motility

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Asian Journal of Andrology 2020年第6期22卷 583-589页

作者： Xiao-hui Li Ran-Ran Chai Guo-Wu Chen Ling-Fei Zhang Wen-Jing Tan-Tai hui-juan shi Patricia A Martin-DeLeon Wai-Sum O Hong Chen Department of Anatomy Histology and EmbryologyKey Laboratory of Medical Imaging Computing and Computer Assisted Intervention of ShanghaiSchool of Basic Medical SciencesShanghai Medical CollegeFudan UniversityShanghai 200032China Shanghai Ji'Ai Genetics and IVF Institute Obstetrics and Gynecology HospitalInstitute of Reproduction and DevelopmentFudan UniversityShanghai 200011China Key Lab of Reproduction Regulation of NPFPC-Shanghai Institute of Planned Parenthood Research Fudan University Reproduction and Development InstitutionShanghai 200032China Department of Biological Sciences University of DelawareNewarkDE 19716-2590USA School of Biomedical Sciences The University of Hong KongHong Kong SARChina

Prohibitin(PHB),an evoluti on arily con served mitochondrial inner membra ne protein,is highly expressed in cells that require strong mitoch on drial ***,we dem on strated that the deleti on of Phb in spermatocytes results in impaired mitochondrial *** addition,PHB expression in the mitochondrial sheath of human sperm has a significantly negative correlation with mitochondrial reactive oxygen species levels,but a positive one with mitochondrial membrane potential and sperm *** results suggest that mitochondrial PHB expression plays a role in sperm ***,the mechanism of PHB-mediated regulation of sperm motility remai ns unk ***,we dem on strate for the first time that PHB interacts with protei n kinase B(AKT)and exists in a complex with phospho-PHB(pT258)and phospho-AKT in the mitochondrial sheath of murine sperm,as determined using colocalization and coimmunoprecipitation *** blocking AKT activity using wortmannin(a phosphatidylinositol 3-kinase[PI3K]inhibitor),murine sperm have significantly(P<0.05)decreased levels of phospho-PHB(pT258)and the total and progressive ***,significantly(P<0.05)lower levels of phospho-PI3K P85 subunit a+γ(pY199 and pY46)and phospho-AKT(pS473;pT308)are found in sperm from infertile asthenospermic and oligoasthenospermic men compared with no rmospermic subjects,which suggest a reduced activity of the PI3K/AKT pathway in these in fertile ***,these sperm from infertile subjects also have a significantly(P<0.05)lower level of phospho-PHB(pT258).Collectively,our findings suggest that the interaction of PHB with AKT in the mitochondrial sheath is critical for sperm motility,where PHB phosphorylation(pT258)level and PI3K/AKT activity are key regulatory factors.

关键词： male in fertility prohibitin(PHB) protein kinase B(AKT) sperm motility

Proteomic analysis of seminal plasma from asthenozoospermia patients reveals proteins that affect oxidative stress responses and semen quality

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Asian Journal of Andrology 2009年第4期11卷 484-491页

作者： Jun Wang Jian Wang Hua-Rong Zhang hui-juan shi Duan Ma Hong-Xin Zhao Biaoyang Lin Run-Sheng Li Systems Biology Division Zhejiang-California Nanosystems Institute （ZCNI） Zhejiang University Zhejiang University Huajiachi Campus Hangzhou 310029 China Key Laboratory of Contraceptive Drugs and Devices of National population and family planning committee （NPFPC） Shanghai Institute of Planned Parenthood Research Shanghai 200030 China Key Laboratory of Molecular Medicine Ministry of Education Institute of Biomedical Sciences Shanghai Medical College Fudan University Shanghai 200030 China

Asthenozoospermia （AS） is a common cause of human male infertility. In one study, more than 80% of the samples from infertile men had reduced sperm motility. Seminal plasma is a mixture of secretions from the testis, epididymis and several male accessory glands, including the prostate, seminal vesicles and Cowper＇s gland. Studies have shown that seminal plasma contains proteins that are important for sperm motility. To further explore the pathophysiological character of AS, we separated the seminal plasma proteins from AS patients and healthy donors using sodium dodecyl sulfate polyacrylamide gel electrophoresis and in-gel digestion, and then subjected the proteins to liquid chromatography-mass spectrometry （LC-MS/MS） analysis. A total of 741 proteins were identified in the seminal plasma, with a false discovery rate of 3.3%. Using spectral counting, we found that 45 proteins were threefold upregulated and 56 proteins were threefold downregulated in the AS group when compared with the control. Most of these proteins originated from the epididymis and prostate. This study identified a rich source of biomarker candidates for male infertility and indicates that functional abnormalities of the epididymis and prostate can contribute to AS. We identified D J-1--a protein that has been shown elsewhere to be involved in the control of oxidative stress （OS）-as a downregulated protein in AS seminal plasma. The levels of D J-1 in AS seminal plasma were about half of those in the control samples. In addition, the levels of reactive oxygen species were 3.3-fold higher in the AS samples than in the controls. Taken together, these data suggest that downregulation of DJ-1 is involved in OS in semen, and therefore affects the quality of the semen.

关键词： asthenozoospermia comparative proteomics DJ-1 seminal plasma

ICSI treatment of severe male infertility can achieve prospective embryo quality compared with IVF of fertile donor sperm on sibling oocytes

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Asian Journal of Andrology 2015年第5期17卷 845-849,I0011页

作者： Ju-Fen Zheng Xiao-Bao Chen Lei-Wen Zhao Min-Zhi Gao Jie Peng Xian-Qin Qu hui-juan shi Xing-Liang Jin China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices SIPPR Shanghai China Department of Reproductive Medicine Renji Hospital Shanghai Jiao Tong University Shanghai China Sydney Centre for Regenerative and Developmental Medicine Kolling Institute for Medical Research Sydney Medical School University of Sydney St. Leonards NSW Australia

Azoospermia, cryptozoospermia and necrospermia can markedly decrease the ability of males to achieve pregnancy in fertile females. However, patients with these severe conditions still have the option to be treated by intracytoplasmic sperm injection （ICSI） to become biological fathers. This study analyzed the fertilization ability and the developmental viabilities of the derived embryos after ICSI treatment of the sperm from these patients compared with in vitro fertilization （IVF） treatment of the proven-fertile donor sperm on sibling oocytes as a control. On the day of oocyte retrieval, the number of sperm suitable for ICSI collected from two ejaculates or testicular sperm extraction was lower than the oocytes, and therefore, excess sibling oocytes were treated by IVF with donor sperm. From 72 couples （73 cycles）, 1117 metaphase Ⅱ oocytes were divided into 512 for ICSI and 605 for IVF. Compared with the control, husbands＇ sperm produced a lower fertilization rate in nonobstructive azoospermia （65.4% vs 83.2%; P〈 0.001）, crytozoospermia （68.8% vs 75.5%; P〈 0.05） and necrospermia （65.0% vs 85.2%; P〈 0.05）. The zygotes derived in nonobstructive azoospermia had a lower cleavage rate （96.4% vs 99.4%; P 〈 0.05）, but the rate of resultant good-quality embryos was not different. Analysis of the rates of cleaved and good-quality embryos in crytozoospermia and necrospermia did not exhibit a significant difference from the control. In conclusion, although the sperm from severe male infertility reduced the fertilization ability, the derived embryos had potential developmental viabilities that might be predictive for the expected clinical outcomes.

关键词： azoospermia cryptozoospermia embryonic development fertilization infertility necrospermia sibling oocytes

Effect of advanced paternal age on reproductive outcomes in IVF cycles of non-male-factor infertility: a retrospective cohort study

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Asian Journal of Andrology 2023年第2期25卷 245-251页

作者： Xin-Mei Lu Yu-Bing Liu Dou-Dou Zhang Xiang Cao Tian-Cheng Zhang Miao Liu hui-juan shi Xi Dong Su-Ying Liu Reproductive Medicine Center Zhongshan HospitalFudan UniversityNo.180 Fenglin RoadShanghai 200032China NHC Key Lab of Reproduction Regulation Shanghai Institute for Biomedical and Pharmaceutical TechnologiesPharmacy SchoolFudan UniversityNo.2140 Xietu RoadShanghai 200032China Center for Reproductive Medicine Tenth People’s Hospital of Tongji University301 Middle Yanchang RoadShanghai 200072China

Advanced paternal age has been overlooked,and its effect on fertility remains *** studies have focused mainly on intracytoplasmic sperm injection(ICSI)cycles in men with ***,few studies have reported on men with semen parameters within reference ***,we conducted a retrospective cohort study analyzing the reproductive outcomes of couples with non-male-factor infertility undergoing in vitro fertilization(IVF)*** total,381 cycles included were subgrouped according to paternal age(0.05).The pregnancy rate was not significantly different in the 35–39-year-old group(42.0%;P>0.05),but was significantly lower in the≥40-year-old group(26.1%;P0.05),but showed a declining *** miscarriage rate significantly increased in the 35–39-year-old group(44.8%)compared with that in the<35-year-old group(21.0%;P<0.05).No abnormality in newborn birth weight was *** results indicated that paternal age over 40 years is a key risk factor that influences the assisted reproductive technology success rate even with good semen parameters,although it has no impact on embryo development.

关键词： embryo development implantation in vitro fertilization paternal age pregnancy sperm

A Cold Model Aerodynamical Test of Air-Staged Combustion in a Tangential Firing Utility Boiler

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International Journal of Plant Engineering and Management 2007年第1期12卷 36-41页

作者： ZHANG hui-juan hui shi-en ZHOU Qu-lan School of Energy and Power Engineering Xi＇an Jiaotong University Xi＇an 710049 P. R. China

The purpose of this paper is to present the flow field in the 300MW tangential firing utility boiler that used the Low NOx Concentric Firing System （LNCFS）. Using the method of cold isothermal simulation ensures the geometric and boundary condition similarity. At the same time the condition of self-modeling is met. The experimental results show that the mixture of primary air and secondary air becomes slower, the average turbulence magnitude the relative diameter of the tangential firing enlarges of the main combustion zone becomes less and when the secondary air deflection angle increases When the velocity pressure ratio of the secondary air to the primary air （p2/p1） enlarges, the mixture of the secondary air and the primary air becomes stronger, the average turbulence magnitude of the main combustion zone increases, and the relative diameter of the tangential firing becomes larger. Because the over fire air （OFA） laid out near the wall has a powerful penetration, the relative diameter of the tangential firing on the section of the OFA is very little, but the average turbulence magnitude is great. When the velocity pressure ratio of the OFA to the primary air POFA/p1 increases, the relative diameter of the tangential firing on the section of the OFA grows little, the average turbulence magnitude becomes larger and the penetration of the OFA becomes more powerful.

关键词： air-staged combustion concentric firing system cold model

MicroRNA-218 is upregulated in gastric cancer after cytoreductive surgery and hyperthermic intraperitoneal chemotherapy and increases chemosensitivity to cisplatin

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World Journal of Gastroenterology 2014年第32期20卷 11347-11355页

作者： Xiang-Liang Zhang hui-juan shi Ji-Ping Wang Hong-Sheng Tang Yin-Bing Wu Zhi-Yuan Fang Shu-Zhong Cui Lian-Tang Wang Department of Abdominal Surgery (Section 2) Affiliated Tumor Hospital of Guangzhou Medical College Department of Pathology The First Affiliated Hospital of Sun Yat-Sen University Department of Surgery Brigham and Women’s Hospital and Harvard Medical School Massachusetts General Hospital and Harvard Medical School Boston MA 02114 United States

AIM: To investigate the molecular mechanisms of miRNA in advanced gastric cancers (AGCs) before and after cytoreductive surgery (CRS) + hyperthermic intraperitoneal chemotherapy (HIPEC).

关键词： Advanced gastric cancer Cytoreductive surgery Hyperthermic intraperitoneal chemotherapy miR-218 Multi-drug resistance MicroRNA

Sperm-specific protein ACTL7A as a biomarker for fertilization outcomes of assisted reproductive technology

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Asian Journal of Andrology 2022年第3期24卷 260-265页

作者： Tian-Ying Yang Ying Chen Guo-Wu Chen Yi-Si Sun Zhi-Chao Li Xiao-Rong Shen Yi-Ni Zhang Wen He Dan Zhou hui-juan shi Ai-Jie Xin Xiao-Xi Sun Shanghai Ji Ai Genetics and IVF Institute Obstetrics and Gynecology HospitalFudan UniversityShanghai 200011China NHC Key Lab of Reproduction Regulation(Shanghai Institute for Biomedical and Pharmaceutical Technologies) School of PharmacyFudan UniversityShanghai 200032China Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Shanghai 200011China Department of Endocrinology and Reproductive Medicine Obstetrics and Gynecology HospitalFudan UniversityShanghai 200011China

Obtaining high-quality embryos is one of the key factors to improve the clinical pregnancy rate of assisted reproductive technologies(ART).So far,the clinical evaluation of embryo quality depends on embryo ***,the clinical pregnancy rate is still ***,new indicators are needed to further improve the evaluation of embryo *** studies have shown that the decrease of sperm-specific protein actin-like 7A(ACTL7A)leaded to low fertilization rate,poor embryo development,and even *** aim of this study was to study whether the different expression levels of ACTL7A on sperm can be used as a biomarker for predicting embryo *** this study,excluding the factors of severe female infertility,a total of 281 sperm samples were collected to compare the ACTL7A expression levels of sperms with high and low effective embryo rates and analyze the correlation between protein levels and in-vitro fertilization(IVF)laboratory *** results indicated that the ACTL7A levels were significantly reduced in sperm samples presenting poor embryo ***,the protein levels showed a significant correlation with fertilization outcomes of ***7A has the potential to be a biomarker for predicting success rate of fertilization and effective embryo and the possibility of embryo *** conclusion,sperm-specific protein ACTL7A has a strong correlation with IVF laboratory outcomes and plays important roles in fertilization and embryo development.

关键词： actin-like 7A assisted reproductive technology biomarker embryo quality in‑vitro fertilization outcomes