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Novel noninvasive quantification of penile corpus caver no sum lesions in hyperlipidemia-induced erectile dysfunction in rabbits by two-dimensional shear-wave elastography

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Asian Journal of Andrology 2019年第2期21卷 143-149页

作者： Jian-Lin Hu hui-xing chen hui-Rong chen Yu Wu Xiao-Wen Sun Zheng Li Jin-Fang xing Department of Reproductive Medicine Shanghai General HospitalShanghai Jiao Tong UniversitySchool of MedicineShanghai 201620China Department of Andrology Center for Men's HealthUrologic Medical CenterShanghai General HospitalShanghai Jiao Tong UniversitySchool of MedicineShanghai 200080China Department of Urology Shanghai General HospitalShanghai Jiao Tong UniversitySchool of MedicineShanghai 201620China Department of Medical Ultrasound Shanghai General HospitalShanghai Jiao Tong UniversitySchool of MedicineShanghai 200080China

Structural alterations in fibroelastic compone nts of the penile corpus caver no usum(CC)may impair its compliance,resulting in venous leakage and erectile dysfunction(ED).Our study evaluated the effectiveness of noninvasive two-dimensional shear-wave elastography(2-D SWE)in quantifying penile CC lesions in rabbits with hyperlipidemia-induced ED.A total of 12 New Zealand white rabbits were randomly divided into two *** were fed a high-cholesterol diet containing 2%cholesterol and 8.5%lard for 10 weeks and the other six were fed normal diet as *** measured the shear-wave elastic quantitative(SWQ)value of penile CC by 2-D *** function was inv estigated by intracavernous injecti on of pa pa ver i ne,and immunohistochemical(IHC)stain inga nd the wester n blot analysis to determine the penile CC *** 10 weeks,the SWQ values obtained from penile CC were remarkably higher in the high-cholesterol-fed compared with the control group,and the AICP(ICP plateau minus ICP baseline)/MAP(ICP:intracavernous pressure,MAP:mean arterial pressure)was markedly *** IHC staining and western blot revealed extracellular matrix(ECM)accumulation in penile cavernous tissues,and the smooth muscle cell(SMC)phenotypic transition was affected,as indicated by reduced alpha-smooth muscle actin and calponinexpression and increased phospho-myosin light chain?。(p-MLC20)/MLC20 and osteopontin *** resulted in ECM accumulation accompanied with SMC phenotypic transition in penile CC and impaired the erectile function *** might,in turn,lead to variations in the SWQ *** suggests that 2-D SWE may be a novel,noninvasive and effective approach that distinguishes penile CC lesions secondary to hyperlipidemia from normal.

关键词： corpus cavernosum erectile dysfunction extracellular matrix accumulation phenotypic transition shear-wave elastography smooth muscle cells

Seminal plasma miR-192a： a biomarker predicting successful resolution of nonobstructive azoospermia following varicocele repair

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Asian Journal of Andrology 2018年第4期20卷 396-399页

作者： Er-Lei Zhi Guo-Qing Liang Peng Li hui-xing chen Ru-hui Tian Peng Xu Zheng Li Department of Andrology Center for Men's Health Department of ART Institute of Urologic Medical Center Shanghai General Hospital Shanghai Key Lab of Reproductive Medicine Shanghai Jiao Tong University 100 Haining Road Shanghai 200080 China Department of Andrology Seventh People's Hospital of Shangha University of TCM Shanghai 200000 China Oriental Jinghua Hospital of Shenyang Department of ART 156 Nanjing Road Shenyang 110005 China

This study was performed to investigate a potential marker for the presence of spermatozoa in the ejaculate following varicocelectomy in Chinese men with nonobstructive azoospermia and varicoceles. The micro-RNA （miR）-192a levels in seminal plasma and testicular tissue were evaluated by quantitative real-time polymerase chain reaction from 60 men with nonobstructive azoospermia and varicoceles （Group A： 27 men with spermatozoa found in the ejaculate after surgery; Group B： 33 men without spermatozoa found in the ejaculate after surgery） and 30 controls. The seminal plasma and testicular tissue miR-192a levels were higher in Group B than in Group A and the controls （P〈 0.001）, and there was no significant difference between Group A and the controls （P〉 0.05）. Apoptosis and proliferation assays with miR mimics and inhibitors showed that miR-192a induced GC-2 cell apoptosis through the activation of Caspase-3 protein. Thus, seminal plasma miR-192a appears to be a potential marker for successfully indicating spermatozoa in the ejaculate following microsurgical varicocelectomy in men with nonobstructive azoospermia and varicoceles. Seminal plasma miR-192a may be a useful clinical marker for prescreening to determine which patients with nonobstructive azoospermia and varicoceles would benefit from varicocelectomy.

关键词： miR-192a nonobstructive azoospermia seminal plasma varicocelectomy

Successful microsurgical vasoepididymostomy for a case of cryptozoospermia

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Asian Journal of Andrology 2022年第4期24卷 436-437页

作者： Ru-hui Tian Yu-Hua Huang hui-xing chen Peng Li Er-Lei Zhi chen-cheng Yao Chao Yang Zheng Li Department of Andrology The Center for Men's HealthUrologic Medical CenterShanghai Key Laboratory of Reproductive MedicineShanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China

Dear Editor,Cryptozoospermia is defined as the apparent absence of spermatozoa from fresh semen samples,but they *** found in centrifuged pellets.1 It is usually caused by a spermatogenic disorder,while cryptozoospermia associated with seminal duct obstruction is less common.2 Because of the extremely low sperm counts in semen samples,men with cryptozoospermia are often reliant on intracytoplasmic sperm injection(ICSI)for paternity.

关键词： injection crypt obstruction

Microsurgical subinguinal varicocelectomy with spermatic cord double traction and vein stripping

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Asian Journal of Andrology 2020年第2期22卷 208-212页

作者： Ru-hui Tian Liang-Yu Zhao hui-xing chen Chao Yang Peng Li Yu-Hua Huang Zhong Wan Er-Lei Zhi chen-cheng Yao Zheng Li Department of Andrology Center for Men’s HealthDepartment of ARTUrologic Medical CenterShanghai General HospitalShanghai Key Lab of Reproductive MedicineShanghai Jiao Tong UniversityShanghai 200080China

We retrospectively reviewed data for 286 patients with varicocele who underwent microsurgical subinguinal varicocelectomy from March 2015 to May 2017 in Shanghai General Hospital(Shanghai,China).In this surgical approach,the testis was delivered,and the gubernacular and external cremasteric veins were *** addition,the spermatic cord was delivered downward with continuous double traction away from the external *** remaining procedure was similar to the conventional *** followed patients for at least 3 months and evaluated postoperative semen parameters,pain symptoms,and *** excluded data for 32 men due to inadequate follow-up(<3 months).Of the remaining 254 patients,73 had oligoasthenospermia,121 had nonobstructive azoospermia,and 60 had symptomatic *** progressive sperm counts increased in the oligoasthenospermic patients from a median preoperative value of 9.15×10^6 ml^?1 to 25.33×10^6 ml^?1(n=34),and 35.6%(26/73)initially oligoasthenospermic men contributed to unassisted *** returned to the ejaculate in 12.4%(15/121)azoospermia *** patients with scrotal pain(n=60),43(71.7%)reported complete resolution of pain,16(26.7%)reported partial resolution,and 1(1.7%)reported no *** patients experienced varicocele *** doubletraction strategy avoids opening the external oblique aponeurosis,and results in less damage and faster *** addition,the stripping strategy eliminates potential damage to the testis caused by the varicose *** results showed that microsurgical subinguinal varicocelectomy using spermatic cord double traction in conjunction with testicular delivery for vein stripping is a safe and effective approach for varicocele repair.

关键词： azoospermia low sperm count sperm motility spermatic cord varicocele

BOD1 regulates the cerebellar IV/V lobe-fastigial nucleus circuit associated with motor coordination

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Signal Transduction and Targeted Therapy 2022年第7期7卷 2490-2503页

作者： Xiu-Xiu Liu xing-hui chen Zhi-Wei Zheng Qin Jiang chen Li Lin Yang Xiang chen xing-Feng Mao Hao-Yang Yuan Li-Li Feng Quan Jiang Wei-xing Shi Takuya Sasaki Kohji Fukunaga Zhong chen Feng Han Ying-Mei Lu Key Laboratory of Cardiovascular&Cerebrovascular Medicine Drug Target and Drug Discovery CenterSchool of PharmacyNanjing Medical University211166 NanjingChina Department of Physiology School of Basic Medical SciencesNanjing Medical University211166 NanjingChina Institute of Pharmacology and Toxicology College of Pharmaceutical SciencesZhejiang University310058 HangzhouChina Department of Pharmaceutical and Administrative Sciences Loma Linda University School of PharmacyLoma LindaCA 92350USA Department of Basic Sciences Loma Linda University School of MedicineLoma LindaCA 92350USA Department of Pharmacology Graduate School of Pharmaceutical SciencesTohoku UniversitySendai 980-8578Japan Institute of Brain Science the Affiliated Brain Hospital of Nanjing Medical University210029 NanjingChina Gusu School Nanjing Medical UniversitySuzhou Municipal HospitalThe Affiliated Suzhou Hospital of Nanjing Medical University215002 SuzhouChina

Cerebellar ataxias are characterized by a progressive decline in motor coordination,but the specific output circuits and underlying pathological mechanism remain poorly *** cell-type-specific manipulations,we discovered a novel GABAergic Purkinje cell(PC)circuit in the cerebellar IV/V lobe that projected to CaMKIIα+neurons in the fastigial nucleus(FN),which regulated sensorimotor ***,transcriptomics profiling analysis revealed various cerebellar neuronal identities,and we validated that biorientation defective 1(BOD1)played an important role in the circuit of IV/V lobe to ***1 deficit in PCs of IV/V lobe attenuated the excitability and spine density of PCs,accompany with ataxia ***,BOD1 enrichment in PCs of IV/V lobe reversed the hyperexcitability of CaMKIIα+neurons in the FN and ameliorated ataxia behaviors in L7-Cre;BOD1f/f ***,these findings further suggest that specific regulation of the cerebellar IV/V lobePCs→FNCaMKIIα+circuit might provide neuromodulatory targets for the treatment of ataxia behaviors.

关键词： fastigial reversed Together

Novel double-layer Silastic testicular prosthesis with controlled release of testosterone in vitro, and its effects on castrated rats

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Asian Journal of Andrology 2017年第4期19卷 433-438页

作者： hui-xing chen Shi Yang Ye Ning Hai-Hao Shao Meng Ma Ru-hui Tian Yu-Fei Liu Wei-Qiang Gao Zheng Li Wei-Liang Xia School of Biomedical Engineering & Med-X Research Institute Shanghai Jiao Tong University Shanghai 200030 China Department of Andrology Urologic Medical Center Shanghai General Hospital Shanghai Jiae Tong University Shanghai 200080 China Shanghai Human Sperm Bank Ren Ji Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200135 China Controlled Release Laboratory Shanghai Institute of Planned Parenthood Research Shanghai 200030 China

Testicular prostheses have been used to deal with anorchia for nearly 80 years. Here, we evaluated a novel testicular prosthesis that can controllably release hormones to maintain physiological levels of testosterone in vivo for a long time. Silastic testicular prostheses with controlled release of testosterone （STPT） with different dosages of testosterone undecanoate （TU） were prepared and implanted into castrated Sprague-Dawley rats. TU oil was applied by oral administration to a separate group of castrated rats. Castrated untreated and sham-operated groups were used as controls. Serum samples from every group were collected to measure the levels of testosterone （T）, follicle-stimulating hormone and luteinizing hormone （LH）. Maximum intracavernous penile pressure （ICPmax） was recorded. The prostates and seminal vesicles were weighed and subjected to histology, and a terminal dexynucleotidyl transferase-mediated UTP nick end labeling （TUNEL） assay was used to evaluate apoptosis. Our results revealed that the weights of these tissues and the levels of T and LH showed significant statistical differences in the oral administration and TU replacement groups compared with the castrated group （P 〈 0.05）. Compared with the sham-operated group, the ICPmax, histology and TUNEL staining for apoptosis, showed no significant differences in the hormone replacement groups implanted with medium and high doses of STPT. Our results suggested that this new STPT could release TU stably through its double semi-permeable membranes with excellent biocompatibility. The study provides a new approach for testosterone replacement therapy.

关键词： controlled-release preparations hormone replacement therapy hypogonadism prosthesis Silastic testosterone

Clinical features and microsurgical reconstruction of congenital unilateral absence of the vas deferens with obstructive azoospermia:a tertiary care center experience

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Asian Journal of Andrology 2023年第1期25卷 73-77页

作者： Yi-Hong Zhou Jian-Jun Dong Er-Lei Zhi chen-cheng Yao Yu-Hua Huang Ru-hui Tian hui-xing chen Ying-Bo Dai Yu-Xin Tang Na-Chuan Liu hui-Rong chen Fu-Jun Zhao Zheng Li Peng Li Department of Andrology The Center for Men's HealthUrologic Medical CenterShanghai General HospitalShanghai Jiaotong University School of MedicineShanghai 200080China Department of Urology The Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhai 519000China Department of Andrology Jiaozuo Maternity and Infant Health HospitalJiaozuo 454000China

Patients with congenital unilateral absence of the vas deferens(CUAVD)manifest diverse symptoms from normospermia to *** for CUAVD patients with obstructive azoospermia(OA)is complicated,and there is a lack of relevant *** this study,we describe the clinical features and evaluate the treatments and outcomes of CUAVD patients with *** December 2015 to December 2020,33 patients were diagnosed as CUAVD with OA in Shanghai General Hospital(Shanghai,China).Patient information,ultrasound findings,semen analysis,hormone profiles,and treatment information were collected,and the clinical outcomes were *** 33 patients,29 patients were retrospectively ***(VE)or cross VE was performed in 12 patients,the patency rate was 41.7%(5/12),and natural pregnancy was achieved in one of the *** other 17 patients underwent testicular sperm extraction as the distal vas deferens(contralateral side)was *** findings showed that VE or cross VE remains an alternative treatment for CUAVD patients with OA,even with a relatively low rate of patency and natural pregnancy.

关键词： congenital unilateral absence of the vas deferens natural pregnancy obstructive azoospermia patency vasoepididymostomy

In-situ Si particle-reinforced joints of hypereutectic Al−60Si alloys by ultrasonic-assisted soldering

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中国有色金属学报 2025年第1期35卷 77-90页

作者： Yuan-xing LI Xiang-bo ZHENG Chao-zheng ZHAO Zong-tao ZHU Yu-jie BAI hui chen Key Laboratory of Advanced Technologies of Materials Ministry of EducationSchool of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China

To improve the wettability of hypereutectic Al−60Si alloy and enhance the mechanical properties of the joints,Al−60Si alloy was joined by ultrasonic soldering with Sn-9Zn solder,and a sound joint with in-situ Si particle reinforcement was *** oxide film of Al−60Si alloy at the interface was identified by transmission electron microscopy(TEM)analysis as amorphous Al_(2)O_(3).The oxide of Si particles in the base metal was also *** oxide film of Al−60Si alloy was observed to be removed by ultrasonic vibration instead of holding *** particle-reinforced joints(35.7 vol.%)were obtained by increasing the ultrasonication *** maximum shear strength peaked at 99.5 MPa for soldering at 330℃with an ultrasonic vibration time of 50 s.A model of forming of Si particles reinforced joint under the ultrasound was proposed,and ultrasonic vibration was considered to promote the dissolution of Al and migration of Si particles.

关键词： hypereutectic Al−60Si alloy ultrasonic-assisted soldering Si particle reinforcement Sn−9Zn solder

Correlation between grain size,mechanical properties and deformed microstructure of Fe-20Mn-6Al-0.6C-0.15Si low-density steel

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Journal of Iron and Steel Research International 2025年第1期32卷 282-292页

作者： Qi Zhang Guang-hui chen Zheng-liang Xue Zheng-kun chen Guang Xu State Key Laboratory of Refractories and Metallurgy Wuhan University of Science and TechnologyWuhan430081HubeiChina Key Laboratory for Ferrous Metallurgy and Resources Utilization of Ministry of Education Wuhan University of Science and TechnologyWuhan430081HubeiChina Red Eagle Novel Refractory Materials Research Institute Zhejiang Red Eagle Group Co.Ltd.Huzhou313000ZhejiangChina

The effects of austenite grain size on the deformed microstructure and mechanical properties of an Fe-20Mn-6Al-0.6C-0.15Si(wt.%)low-density steel were *** microstructure of the experimental steel after solution treatment was single austenitic *** austenite grain size increased with solution temperature and time.A model was established to show the relationship between temperature,time and austenite grain size for the experimental *** addition,as the solution temperature increased,the strength decreased,while the elongation first increased and then *** decrease in elongation after solution treatment at 1100℃ for 90 min is contributed to the over-coarse austenite ***,after solution treatment at 900℃ for 90 min,the strength-elongation product reached the highest value of 44.4 GPa%.As the austenite grain size increased,the intensity of//tensile direction fiber *** was accompanied by a decrease in dislocation density,resulting in a lower fraction of low-angle grain boundaries and a lower work hardening ***,the austenite grain size has a critical influence on the mechanical properties of the low-density *** grains lead to a lower yield strength due to the Hall-Petch effect and a lower tensile strength because of lower dislocation strengthening.

关键词： Austenitic low-density steel Solution treatment Grain size Mechanical property Deformed microstructure