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Effects of sampling strategies and DNA extraction methods on eDNA metabarcoding: A case study of estuarine fish diversity monitoring

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Zoological Research 2022年第2期43卷 192-204页

作者： hui-ting ruan Rui-Li Wang Hong-ting Li Li Liu Tian-Xu Kuang Min Li Ke-Shu Zou University Joint Laboratory of Guangdong Province Hong Kong and Macao Region on Marine Bioresource Conservation and ExploitationCollege of Marine ScienceSouth China Agricultural UniversityGuangzhouGuangdong 510642China Key Laboratory for Sustainable Utilization of Open-sea Fishery Ministry of Agriculture and Rural AffairsSouth China Sea Fisheries Research InstituteChinese Academy of Fishery SciencesGuangzhouGuangdong 510300China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) GuangzhouGuangdong 511458China Guangzhou CAOMUFAN Ecological Research limited company GuangzhouGuangdong 510520China College of Marine Sciences Shanghai Ocean UniversityShanghai 201306China

Environmental DNA(eDNA)integrated with metabarcoding is a promising and powerful tool for species composition and biodiversity assessment in aquatic ecosystems and is increasingly applied to evaluate fish *** date,however,no standardized eDNA-based protocol has been established to monitor fish *** this study,we investigated and compared two filtration methods and three DNA extraction methods using three filtration water volumes to determine a suitable approach for eDNA-based fish diversity monitoring in the Pearl River Estuary(PRE),a highly anthropogenically disturbed estuarine *** to filtration-based precipitation,direct filtration was a more suitable method for eDNA metabarcoding in the *** combined use of DNeasy Blood and Tissue Kit(BT)and traditional phenol/chloroform(PC)extraction produced higher DNA yields,amplicon sequence variants(ASVs),and Shannon diversity indices,and generated more homogeneous and consistent community composition among *** to the other combined protocols,the PC and BT methods obtained better species detection,higher fish diversity,and greater consistency for the filtration water volumes of 1000 and 2000 mL,*** eDNA metabarcoding protocols were more sensitive than bottom trawling in the PRE fish surveys and combining two techniques yielded greater taxonomic ***,combining traditional methods with eDNA analysis enhanced *** results indicate that methodological decisions related to eDNA metabarcoding should be made with caution for fish community monitoring in estuarine ecosystems.

关键词： eDNA metabarcoding Fish diversity Sampling strategies DNA extraction Estuarine ecosystem

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Constructing a 3D multichannel structure to enhance performance of Ni–Co–Mn hydroxide electrodes for flexible supercapacitors

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Rare Metals 2024年第1期43卷 148-163页

作者： Zhi-hui Xu Shi-Shuai Sun Xue-Lei Li Zhi-hui Zhang Hai-Ying Li Shuang-ting ruan Shou-Gen Yin Key Laboratory of Display Materials and Photoelectric Devices(Ministry of Education)and Tianjin Key Laboratory for Photoelectric Materials and Devices School of Materials Science and EngineeringTianjin University of TechnologyTianjin 300384China College of Science Tianjin University of TechnologyTianjin 300384China School of Materials Science and Engineering Inner Mongolia University of TechnologyHohhot 010051China

The balancing of the electrochemical performance,mechanical stability,and processing technology for applying supercapacitors to flexible and wearable electronics continues to encounter severe ***,we prepare Ni-Co-Mn hydroxide electrodes with a threedimensional multichannel structure via a simple hydrothermal *** are constructed using vertically contiguous nano sheets with a uniform thickness and rough *** electrodes can provide numerous electroactive sites and accelerate the transmission of electrolyte *** relationship between the structure and electrochemical performances is verified by experiments and theoretical ***-dimensional(2D)planar and one-dimensional(1D)fiber electrodes are prepared using a flexible carbon cloth(CC)and carbon fiber(CF),respectively,as *** assembled quasi-solid-state flexible asymmetric supercapacitor(FASC)with a twodimensional sandwich structure using NiCoMn-OH/CC as the electrode achieves a remarkable energy density of73.8 Wh·kg^(-1)at a power density of 1.03 kW·kg^(-1).The quasi-solid-state FASC with a 1D linear structure using NiCoMn-OH/CF as the electrode also attains a high energy density(12.9 Wh·kg^(-1)at a power density of0.75 W·kg^(-1)).Moreover,the electrochemical performances of the NiCoMn/CC//AC/CC and NiCoMn/CF//AC/CF FASCs are not disturbed at different bending angles(0°,45°,90°,135°and 180°),This indicates the superior flexibility of the *** also assemble a self-powered energy-harvesting storage system by integrating FASCs and commercial solar cells to verify its *** displays sustainable development potential for energy storage.

关键词： Supercapacitors Flexible Ni–Co–Mn hydroxide 3D multichannel structure High performances

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Porous Organic Cage‑Based Quasi‑Solid‑State Electrolyte with Cavity‑Induced Anion‑Trapping Effect for Long‑Life Lithium Metal Batteries

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Nano-Micro Letters 2025年第2期17卷 376-386页

作者： Wei-Min Qin Zhongliang Li Wen‑Xia Su Jia‑Min Hu Hanqin Zou Zhixuan Wu Zhiqin ruan Yue‑Peng Cai Kang Li Qifeng Zheng School of Chemistry South China Normal UniversityGuangzhou 510006People’s Republic of China Key Laboratory of Functional Metal‑Organic Compounds of Hunan Province College of Chemistry and Material ScienceHengyang Normal UniversityHengyang 421008People’s Republic of China Guangzhou Key Laboratory of Energy Conversion and Energy Storage Materials Guangzhou 510006People’s Republic of China

Porous organic cages(POCs)with permanent porosity and excellent host–guest property hold great potentials in regulating ion transport behavior,yet their feasibility as solid-state electrolytes has never been testified in a practical ***,we design and fabricate a quasi-solid-state electrolyte(QSSE)based on a POC to enable the stable operation of Li-metal batteries(LMBs).Benefiting from the ordered channels and cavity-induced anion-trapping effect of POC,the resulting POC-based QSSE exhibits a high Li+transference number of 0.67 and a high ionic conductivity of 1.25×10^(−4) S cm^(−1) with a low activation energy of 0.17 *** allow for homogeneous Li deposition and highly reversible Li plating/stripping for over 2000 *** a proof of concept,the LMB assembled with POC-based QSSE demonstrates extremely stable cycling performance with 85%capacity retention after 1000 ***,our work demonstrates the practical applicability of POC as SSEs for LMBs and could be extended to other energy-storage systems,such as Na and K batteries.

关键词： Porous organic cage Cavity-induced anion-trapping Quasi-solid-state electrolyte Homogeneous Li+flux Lithium metal battery

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The Development Trend of Developed Countries to Invest in Education of Disadvantaged Groups and the Enlightenment to Our Country

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学术界 2013年第9期 288-297页

作者： ruan ting Song Zhanmei the College of Early Childhood Education and Special Education China Normal University Shanghai 200062 the Research Institute of Early Childhood Education Shangdong Yingcai University Jinan Shangdong 250104

Educational investment is the material basis for developing intelligence and educational cause and also an important factor for the development of economic and *** paper argues that educational investment is an important strategy to solve the problem of disadvantaged groups in our country,and hopes that through the analysis of the structure and the trend for educational investment in developed countries,and it can provide reference and valuable suggestions for our country.

关键词：教育投资弱势群体发达国家发展趋势物质基础社会发展

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Energy and Laplacian of fractal interpolation functions

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Applied Mathematics(A Journal of Chinese Universities) 2017年第2期32卷 201-210页

作者： LI Xiao-hui ruan Huo-jun School of Mathematical Science Zhejiang UniversityHangzhou 310027China.

Abstract. In this paper, we first characterize the finiteness of fractal interpolation functions （FIFs） on post critical finite self-similar sets. Then we study the Laplacian of FIFs with uniform vertical scaling factors on the Sierpinski gasket （SG）. As an application, we prove that the solution of the following Dirichlet problem on SG is a FIF with uniform vertical scaling factor 1/5 ：△=0 on SG ／ {q1, q2, q3}, and u（qi）=ai, i = 1, 2, 3, where qi, i=1, 2, 3, are boundary points of SG.

关键词： Dirichlet problem fractal interpolation function Sierpinski gasket energy Laplacian.

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Laser direct writing of flexible multifunctional airflow sensors on the Kevlar fabric

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Nano Research 2025年第1期18卷 443-454页

作者： Wei Wang Zi-Qing Chen Yong-Qi Li Yu-Long Wang Mei-Chen Liu Yun-Bo ruan Yang Zhang Shu-Juan Liu Qiang Zhao College of Electronic and Optical Engineering and College of Flexible Electronics(Future Technology) Nanjing University of Posts and TelecommunicationsNanjing 210023China State Key Laboratory of Organic Electronics and Information Displays and Jiangsu Key Laboratory for Biosensors Institute of Advanced Materials(IAM)Nanjing University of Posts and TelecommunicationsNanjing 210023China Zhe Jiang Topsun Logistic Control CO. LTD.Yuhuan 317600China Department of Rehabilitation Medicine School of Acupuncture-Moxibustion and Tuina and School of Health Preservation and RehabilitationNanjing University of Chinese MedicineNanjing 210023China

The growing interest in flexible devices has emerged as a global trend due to their advantages in flexibility,lightweight structure,and wearability,addressing the limitations of traditional *** wearable airflow sensors have been previously reported,the development of flexible fabric-based airflow sensors capable of functioning in environments with open flames—critical for fire rescue operations—has yet to be explored,largely due to the poor fire resistance of conventional *** this work,we first present a flexible,wearable,and multifunctional airflow sensor with excellent fire-resistant properties,fabricated through a simple direct laser writing *** sensor maintains airflow detection capabilities even in the presence of open ***,the fabrication of fabric-based sensors involves complex procedures such as carbon materials doping or vapor-phase deposition,leading to lengthy preparation cycles and high ***,fabric-based devices are inherently prone to *** address these challenges,we introduce twice-vertical laser-induced graphene(TVLIG)as a sensitive and reliable component for fire-resistant airflow *** resulting TVLIG/Kevlar fabric can be integrated into various garments,particularly protective suits,to form sensitive and fire-resistant airflow sensors capable of detecting airflow velocity and direction in both two-dimensional(2D)and three-dimensional(3D)spaces during fire ***,the TVLIG patterns can be expanded to multifunctional platforms,such as glucose detection for injured individuals,offering further applications in rescue *** functional expansion reduces the burden on rescue personnel and streamlines device *** its outstanding sensing capabilities,fire resistance,and expandability,the developed flexible airflow sensor shows great potential for various real-world rescue scenarios,promising advancements in wearable sensing technology for rescue enginee

关键词： flexible airflow sensor direct laser writing twice-vertical laser-induced graphene fire accident rescue Kevlar fabric

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Selective introduction of surface defects in anatase TiO2 nanosheets for highly efficient photocatalytic hydrogen generation

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Rare Metals 2022年第6期41卷 2074-2083页

作者： Jiang-Yuan Qiu Hua-Zhang Feng Zhao-hui Chen Shu-Hong ruan Yan-Ping Chen ting-ting Xu Jing-Yun Su En-Na Ha Lu-Yang Wang College of New Materials and New Energies Shenzhen Technology UniversityShenzhen 518118China College of Health Scienceand Environmental Engineering Shenzhen Technology UniversityShenzhen 518118China

Defect engineering greatly enhances the cat-alytic activity of transition metal semiconductor ***,localized surface defects engineering has been intensively researched,but it still remains challenges on how to tilt the balance to the controllable construction of surface defects rather than bulk ***,we report a facile room-temperature solution processing strategy on(001)facet exposed anatase TiO_(2) nanosheets(ATO),in which localized defects are generated on the surface selectivity with high *** achieve the aspect,lithium-ethylenediamine(Li-EDA)treatment is carried out on(001)facet exposed ATO under a mild *** optimized sample exhibits outstanding photocatalytic H_(2) production rates of 9.28 mmol·g^(-1)·h^(-1) with loading 0.5 wt%Pt as co-catalyst(AM 1.5),which is nearly 7.5 times higher than that of the pristine *** defect engi-neering strategy of ATO photocatalyst will spark the ideas for the defects engineering and semiconductor photocata-lyst,which is with important application prospect in solar energy conversion,including hydrogen generation and carbon dioxide reduction.

关键词： Defeet engineering Selective reaction Anatase titanium oxide Disorder structure Hydrogen generation

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Regularity of Global Attractor for Atmospheric Circulation Equations with Humidity Effect

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Acta Mathematicae Applicatae Sinica 2019年第4期35卷 820-829页

作者： Jiao-jiao PAN Qian JIANG ting-wei ruan Hong LUO School of Mathematical Sciences Sichuan Normal University

In this article, regularity of the global attractor for atmospheric circulation equations with humidity effect is considered. It is proved that atmospheric circulation equations with humidity effect possess a global attractor in H^k(?, R^4) for any k≥0, which attracts any bounded set of H^k(Ω, R^4) in the H^k-norm. The result is established by means of an iteration technique and regularity estimates for the linear semigroup of operator, together with a classical existence theorem of global attractor.

关键词： global attractor regularity atmospheric circulation equations humidity effect

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Visible region extraction from a sequence of rational Bézier surfaces

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2006年第7期7卷 1233-1240页

作者： ruan Xiao-yu ZHANG hui YONG Jun-hai Department of Computer Science and Technology Tsinghua University Beijing 100084 China School of Software Tsinghua University Beijing 100084 China

A method for computing the visible regions of free-form surfaces is proposed in this paper. Our work is focused on accurately calculating the visible regions of the sequenced rational Bézier surfaces forming a solid model and having coincident edges but no inner-intersection among them. The proposed method calculates the silhouettes of the surfaces without tessellating them into triangle meshes commonly used in previous methods so that arbitrary precision can be obtained. The computed sil- houettes of visible surfaces are projected onto a plane orthogonal to the parallel light. Then their spatial relationship is applied to calculate the boundaries of mutual-occlusion regions. As the connectivity of the surfaces on the solid model is taken into account, a surface clustering technique is also employed and the mutual-occlusion calculation is accelerated. Experimental results showed that our method is efficient and robust, and can also handle complex shapes with arbitrary precision.

关键词： Rational Bézier surface Visibility Self-occlusion Mutual-occlusion

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Tip-Nanoparticle Near-Field Coupling in Scanning Near-Field Microscopy by Coupled Dipole Method

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Chinese Physics Letters 2018年第4期35卷 42-45页

作者： Yi ruan Kan Li Qiang Lin ting Zhang Collaborative Innovation Center for Bio-Med Physics Information Technology College of Science Zhejiang University of Technology Hangzhou 310023 College of Information Science ＆ Electronic Engineering Zhejiang Provincial Key Laboratory of Information Processing Communication and Networking （IPCN） Zhejiang University Hangzhou 310000

We use the couple dipole method to investigate the scanning near-field optical microscopy metallic tip-nanoparticle near-field interaction. Dependences of the local field intensity inside the nanoparticle on the nanosized tip shape,the tip open angle and the illumination angle are revealed. In combination with the previous results, we establish a complete model to understand the tip-nanoparticle near-field coupling mechanism.

关键词： Tip-Nanoparticle Near-Field Coupling in Scanning Near-Field Microscopy by Coupled Dipole Method

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