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Nitric Oxide Blocks Blue light-Induced K^+ Influx by Elevating the Cytosolic Ca^(2+) Concentration in Vicia faba L. Guard Cells

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Journal of Integrative Plant Biology 2013年第6期55卷 527-536页

作者： Xiang Zhao Yuan-Yuan li hui-li xiao Chang-Shui Xu xiao Zhang Institute of Plant Stress Biology State Key Laboratory of Cotton Biology College of Life Sciences Henan University

Ca2＋ plays a pivotal role in nitric oxide （NO）-promoted stomatal closure. However, the function of Ca2＋ in NO inhibition of blue light （BL）-induced stomatal opening remains largely unknown. Here, we analyzed the role of Ca2＋ in the crosstalk between BL and NO signaling in Vicia faba L. guard cells. Extracellular Ca2＋ modulated the BL-induced stomatal opening in a dose-dependent manner, and an application of 5 μM Ca2＋ in the pipette solution significantly inhibited BL-activated K＋ influx. Sodium nitroprusside （SNP）, a NO donor, showed little effect on BL-induced K＋ influx and stomatal opening response in the absence of extracellular Ca2＋ , but K＋ influx and stomatal opening were inhibited by SNP when Ca2＋ was added to the bath solution. Interestingly, although both SNP and BL could activate the plasma membrane Ca2＋ channels and induce the rise of cytosolic Ca2＋ , the change in levels of Ca2＋ channel activity and cytosolic Ca2＋ concentration were different between SNP and BL treatments. SNP at 100 μM obviously activated the plasma membrane Ca2＋ channels and induced cytosolic Ca2＋ rise by 102.4%. In contrast, a BL pulse （100 μmol/m 2 per s for 30 s） slightly activated the Ca2＋ channels and resulted in a Ca2＋ rise of only 20.8%. Consistently, cytosolic Ca2＋ promoted K＋ influx at 0.5 μM or below, and significantly inhibited K＋ influx at 5 μM or above. Taken together, our findings indicate that Ca2＋ plays dual and distinctive roles in the crosstalk between BL and NO signaling in guard cells, mediating both the BL-induced K＋ influx as an activator at a lower concentration and the NO-blocked K＋ influx as an inhibitor at a higher concentration.

关键词： Blue light cytosolic Ca2+ nitric oxide stomatal movement.

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Ionic liquids as efficient phase-transfer catalysts for the solid base-promoted monoalkylation of diethyl malonate

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Chinese Chemical Letters 2007年第3期18卷 279-282页

作者： hui Sun Juan li xiao Chen Cai Dong Jiang li Yi Dai Department of Chemistry East China Normal University Shanghai 200062 China

Ionic liquids （ILs） based on 1,3-dialkylimidazolium and tetraalkylammonium cations were employed as a series of efficient, environmentally benign phase-transfer catalysts （PTCs） for the base-promoted monoalkylation of diethyl malonate. The influence of various heterogeneous bases on yields was studied. Good yields and high selectivity were obtained. Solvent-free, mild reaction condition, short reaction time, and easy purification were the merits of this method. The catalytic system （IL-hase） could also be recycled after the extraction of products with ether.

关键词： Ionic liquids Phase-transfer catalysis Diethyl malonate Alkylation

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Numerical analysis of factors affecting the range of heat transfer in earth surrounding three subways

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Journal of China University of Mining and Technology 2008年第1期18卷 67-71页

作者： HU Zeng-hui li xiao-zhao ZHAO xiao-bao xiao lin WU Wei Department of Earth Sciences Nanjing University Nanjing Jiangsu 210093 China

In order to examine the factors which affect the range of heat transfer in earth surrounding subways, FLAC3D was adopted in this study to analyze these factors, under different conditions, in a systematic manner. When we compare these numerical tests, the results show that the main factors, affecting the heat transfer range are the thermal properties of the surrounding earth, the initial ground temperature and the temperature in the tunnel. The heat transfer coefficient between air and linings has little effect on the temperature distribution around the tunnel. The current results can provide a reference for improving the thermal environment in subways and optimizing the design of subwav ventilation and air conditioning.

关键词： earth surrounding subways range of heat transfer factors affecting heat transfer numerical analysis

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Effects of two novel nucleoside analogues on different hepatitis B virus promoters

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World Journal of Gastroenterology 2008年第12期14卷 1836-1841页

作者： Xing-Xing He Ju-Sheng lin Ying Chang Ying-hui Zhang Yan li xiao-Yan Wang Dong Xu xiao-Ming Cheng Instituteof liver diseases Tongji Hospital of Tongji Medical Co11~g~Huazhong University of Science and Technology Wuhan 430030Hubei Province China

AIM： To explore the effects of the nucleoside analogues β-L-D4A and β-LPA on hepatitis B virus （HBV） promoters. METHODS： Four HBV promoters were amplified by polymerase chain reaction （PCR） and subcloned into the expression vector pEGFP-1. The four recombinants controlled by HBV promoters were confirmed by restriction analysis and sequencing. Human hepatoma HepG2 cells transfected with the recombinant plasmids were treated with various concentrations of β-L-D4A and β-LPA. Then, enhanced green fluorescent protein （EGFP）-positive cells were detected by fluorescence microscopy and using a fluorescence activated cell sorter RESULTS： Four HBV promoters were separately obtained and successfully cloned into pEGFP-1, Expression of EGFP under the control of the surface promoter （Sp） and the X promoter （Xp） was inhibited by β-L-D4A in a dosedependent manner, while expression of EGFP under the control of the core promoter （Cp） and Xp was inhibited by β-LPA in a dose-dependent manner. CONCLUSION： The two novel nucleoside analogues investigated here can inhibit the activities of HBV promoters in a dose-dependent manner. These findings may explain the mechanisms of action by which these two novel compounds inhibit HBV DNA replication.

关键词： Hepatitis B virus Nucleoside analogue Hepatitis B virus promoter Enhanced green fluorescentprotein Fluorescence activated cell sorter

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RELATIONSHIP BETWEEN HEMOGLOBIN A1C AND BLOOD GLUCOSE THROUGHOUT THE DAY IN WELL-GLYCEMIC-CONTROLLED MEDICAL NUTRITION THERAPY ALONE TYPE 2 DIABETIC PATIENTS

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Chinese Medical Sciences Journal 2006年第2期21卷 90-94页

作者： Wen-hui li Xin-hua xiao Qi Sun Guo-hua Yang Heng Wang Department of Endocrinology Peking Union Medical College Hospital Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730

Objective To investigate the relationship between glycosylated hemoglobin A1 c （HbA1 c） and blood glucose levels of eight different points throughout the day in well-glycemic-controlled medical nutrition therapy （MNT） alone type 2 diabetic pafients. Methods Data were collected as＂ capillary blood glucose value of eight different sample points among sixteen observing days in thirty MNT alone type 2 diabetic patients. The correlation between HbAI c and capillary blood glucose value was evaluated by Pearson＇s correlation method. Results The r-values between HbA1c and capillary blood glucose of 3：00, 6：00, and bedtime （22：00-23：00） were 0. 81,0. 79, and 0. 78, respectively（P 〈0. 001 ）. The best correlation was found between the mean value of 8- point blood glucose value throughout the day and HbA1c （ r=0. 84, P 〈0. 001 ）. Conclustion Fasting blood glucose and postabsorptive blood glucose have better correlations with HbAlc compared with other points in this group of well-glycemic-controlled MNT alone type 2 diabetic patients.

关键词： type 2 diabetes medical nutrition therapy hemoglobin Alc blood glucose

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Hypoadiponectinemia predicts impaired endothelium-independent vasodilation in newly diagnosed type 2 diabetic patients： an 8-year prospective study

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Chinese Medical Journal 2011年第22期124卷 3607-3612页

作者： li hui xiao Yang liU hui CHEN xiao-yan li Xin-ying TANG Wei-li liU Shi-ping XU Ai-min ZHOU Zhi-guang Diabetes Center Second Xiangya Hospital and Institute of Metabolism and Endocrinology Key Laboratory of Diabetes Immunology Ministry of Education Central South University Changsha Hunan 410011 China

Background Adiponectin is an adipokine with insulin-sensitising and anti-atherogenic properties. The aim of this study was to investigate whether low adiponectin levels predict the impairment of endothelial function in newly diagnosed type 2 diabetic patients in an 8-year prospective study. Methods In the prospective study, we enrolled 133 newly diagnosed type 2 diabetic patients without subclinical atherosclerosis and gave them intensive therapy; the mean treatment period was 8 years. Intensive treatment was a stepwise implementation of behavior modification and pharmacological therapy targeting hyperglycaemia, hypertension, dyslipidaemia and obesity. We measured baseline circulating adiponectin with an enzyme-linked immunosorbent assay, endothelium-dependent and -independent vasodilation by high-resolution vascular ultrasound. At year 8, 102 patients were reexamined for endothelium-dependent and -independent vasodilation. Results Sex-adjusted adiponectin level was positively correlated with endothelium-independent vasodilation both at baseline （r=0.150, P=0.043） and at year 8 （r=0.339, P=0.001）, whereas no association was found between adiponectin and endothelium-dependent vasodilation. In a stepwise multivariate linear regression model, adiponectin was an independent predictor for impaired endothelium-independent vasodilation at year 8 （P=0.001）. Conclusions Plasma adiponectin concentration was associated with endothelium-independent vasodilation and hypoadiponectinemia predicted the impairment of endothelium-independent vasodilation in newly diagnosed type 2 diabetic patients under multifactorial intervention. These data support the causative link of impairment of endothelium-independent vasodilation with hypoadiponectinemia.

关键词： type 2 diabetes endothelium vasodilation adiponectin

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Treatment of 84 Cases of Shoulder Periarthritis by Tuina plus Acupuncture

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Journal of Acupuncture and Tuina Science 2003年第2期1卷 47-49页

作者： li hui-min xiao Yuan-chun Traumatological Department Seaman Hospital Shanghai 200080 China

Acupuncture, Tuina and combination of acupuncture and Tuina were used to treat 250 cases of shoulder periarthritis who were randomly divided into three groups. Bingfeng (Sl 12) and Ashi points were selected and cupping therapy was also given following acupuncture in acupuncture group; pressing, kneading and plucking manipulations were performed in Tuina group, acupuncture plus Tuina were employed in group of acupuncture plus Tuina. The therapeutic results showed acupuncture plus Tuina was better than simple Tuina, and statistical analyses demonstratedPxiao Yuan-chun

关键词： Shoulder Joint Inflamanation Acupuncture Therapy Tuina Massage

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Decoding the nexus:branched-chain amino acids and their connection with sleep,circadian rhythms,and cardiometabolic health

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Neural Regeneration Research 2025年第5期20卷 1350-1363页

作者： hui li Laurent Seugnet Department of Neurology Xijing HospitalXi’anShaanxi ProvinceChina Centre de Recherche en Neurosciences de Lyon Integrated Physiology of the Brain Arousal Systems(WAKING)UniversitéClaude Bernard Lyon 1INSERM U1028CNRS UMR 5292BronFrance

The sleep-wake cycle stands as an integrative process essential for sustaining optimal brain function and,either directly or indirectly,overall body health,encompassing metabolic and cardiovascular *** the heightened metabolic activity of the brain,there exists a considerable demand for nutrients in comparison to other *** these,the branched-chain amino acids,comprising leucine,isoleucine,and valine,display distinctive significance,from their contribution to protein structure to their involvement in overall metabolism,especially in cerebral *** the first amino acids that are released into circulation post-food intake,branched-chain amino acids assume a pivotal role in the regulation of protein synthesis,modulating insulin secretion and the amino acid sensing pathway of target of ***-chain amino acids are key players in influencing the brain's uptake of monoamine precursors,competing for a shared *** their involvement in protein synthesis,these amino acids contribute to the metabolic cycles ofγ-aminobutyric acid and glutamate,as well as energy ***,they impact GABAergic neurons and the excitation/inhibition *** rhythmicity of branchedchain amino acids in plasma concentrations,observed over a 24-hour cycle and conserved in rodent models,is under circadian clock *** mechanisms underlying those rhythms and the physiological consequences of their disruption are not fully *** sleep,obesity,diabetes,and cardiovascular diseases can elevate branched-chain amino acid concentrations or modify their oscillatory *** mechanisms driving these effects are currently the focal point of ongoing research efforts,since normalizing branched-chain amino acid levels has the ability to alleviate the severity of these *** this context,the Drosophila model,though underutilized,holds promise in shedding new light on these *** findings indicate its po

关键词： branched-chain amino acids cardiovascular health circadian clock drosophila insulin metabolism sleep γ-aminobutyric acid

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Chilling Tolerance of Cucumber During Germination is Related to Expression of Lysine Decarboxylase Gene

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Agricultural Sciences in China 2005年第12期4卷 898-902页

作者： LU Ming-hui li xiao-ming CHEN Jin-feng CHEN Long-zheng QIAN Chun-tao National Key Laboratory of Crop Genetics and Germplasm Enhancement College of Horticulture Nanjing Agricultural UniversityNanjing 210095 P.R.China

Using cDNA-AFLP technique, a specific fragment was isolated from cucumber cultivar Changchun mici possessing chilling tolerance induced at low temperature （15℃）. This fragment, named cctr 132, could not be induced in the chilling sensitive cucumber cultivar Beijing jietou. After recovering the fragment, sequencing and translating, the results of blastx and blastp in GenBank of NCBI indicated that CCTR132 had 88.37% identities and 100% positives with Oryza sativa putative lysine decarboxylase-like protein respectively, and PGGXGTXXE, the putative conserved domain of lysine decarboxylase family, was detected from CCTR132, suggesting the cucumber chilling tolerance during germination is related to the expression of the lysine decarboxylase gene.

关键词： Cucumber Chilling tolerance cDNA-AFLP Lysine decarboxylase Cadaverine

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Mechanisms of improvement of left ventricle remodeling by transplanting two kinds of autologous bone marrow stem cells in pigs

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Chinese Medical Journal 2008年第23期121卷 2403-2409页

作者： li Shu-ren QI xiao-yong HU Fu-li ZHANG Jian-qing WANG Tian-hong DANG Yi MENG Cun-liang liU hui-liang li Ying-xiao WU Di DONG Jie XUN li-ying GAO li-hui JIN Fu-chang Department of Cardiology People's Hospital of Hebei Province Shijiazhuang Hebei 050051 China

Background The necrosis of a large number of myocardial cells after acute myocardial infarction （AMI） results in a decrease of cardiac function and ventricle remodeling. Stem cell transplantation could improve cardiac function after AMI, but the involving mechanisms have not been completely understood. The present study aimed to investigate the effects of transplantation of autologous bone marrow mononuclear cells （BM-MNC） and mesenchymal stem cells （MSCs） via the coronary artery on the ventricle remodeling after AMI as well as the mechanisms of the effects of transplantation of different stem cells on ventricle remodeling. Methods A total of 36 male pigs were enrolled in this study, which were divided into 4 groups： control group, simple infarct model group, BM-MNC transplantation group, and MSCs transplantation group. At 90 minutes when a miniature porcine model with AMI was established, transplantation of autologous BM-MNC （（4.7±1.7）×10^7） and MSCs （（6.2±1.6）×10^5） was performed in the coronary artery via a catheter. Ultrasound, electron microscope, immunohistochemical examination and real time reverse transcriptase-pelymerase chain reaction were used respectively to observe cardiac functions, counts of blood vessels of cardiac muscle, cardiac muscle nuclear factor （NF）-κB, myocardial cell apoptosis, and the expression of the mRNA of vascular endothelial growth factor （VEGF） and basic fibroblast growth factor （bFGF） in cardiac muscles. Multivariate Logistic regression was used to analyze the correlation factors of left ventricular end-diastolic diameter （EDD）. Results The number of blood vessels in the infarct zone and around its border in the BM-MNC transplantation group was more than those in the infarct model group and MSCs group （P=0.0001） and there was less myocardial cell apoptosis in the stem cell transplantation group than that in the infarct model group （all P 〈0.01）. The positive rate of NF-κB in the stem cell transplantation group was lower than th

关键词： bone marrow mononuclear cell bone marrow mesenchymal stem cell cellular transplantation acute myocardial infarction ventricle remodeling

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